Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,494,473	A→G	7.0%	intergenic (‑67/‑150)	idnD ← / → idnK	L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,494,473	0	A→G	7.0%	3.3 0.0 ‑0.2	57	intergenic (‑67/‑150)	idnD/idnK	L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (23/30):  total (25/32)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.95e-01

ATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAAAAATATAGATAAAATG  >  NC_000913/4494178‑4494760                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                atgaGTTGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAg                                                                                                                                                                                                                                                                                            <  1:805661/301‑1 (MQ=255)              aaTAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGaaa                                                                                                                                                                                                                                                                               <  2:948782/301‑1 (MQ=255)                  aCTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTg                                                                                                                                                                                                                                                                           >  2:106478/1‑301 (MQ=255)                              ccTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTAGGGTTATTTGTACTAATGTTCCATTAGTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTAt                                                                                                                                                                                                                                                               <  1:401423/300‑1 (MQ=255)                               cTTCATGACCTAACATCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGTTAATAATGTAAATCGGAACCGCAAATTCCACCTCGTGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTGTTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATg                                                                                                                                                                                                                                                              <  2:733928/301‑1 (MQ=255)                               cTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTAtt                                                                                                                                                                                                                                                              >  1:601522/1‑300 (MQ=255)                                ttCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGt                                                                                                                                                                                                                                                             <  2:455227/301‑1 (MQ=255)                                  cATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTa                                                                                                                                                                                                                                                           <  2:931251/301‑1 (MQ=255)                                  cATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTa                                                                                                                                                                                                                                                           <  2:739233/301‑1 (MQ=255)                                      aCCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGt                                                                                                                                                                                                                                                             <  2:713147/295‑1 (MQ=255)                                      aCCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCa                                                                                                                                                                                                                                                       >  1:954999/1‑301 (MQ=255)                                      aCCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGAATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACTTCAGAAAATTGTTACATTACTATGtctgtat                                                                                                                                                                                                                                                       >  1:713132/1‑295 (MQ=255)                                      aCCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCAC‑TGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCAt                                                                                                                                                                                                                                                      >  1:242800/1‑301 (MQ=255)                                       ccTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCAt                                                                                                                                                                                                                                                      <  2:601528/301‑1 (MQ=255)                                       ccTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGAGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTa                                                                                                                                                                                                                                                           <  2:54873/296‑1 (MQ=255)                                       ccTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGAGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTActgtt                                                                                                                                                                                                                                                      >  1:54872/1‑297 (MQ=255)                                          aaCACCACCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTTCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTCTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTAGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCTTAATTTGTGATGTAGAACACGTACGAAAATTGTTACATTACTATGTTACGAATaaa                                                                                                                                                                                                                                                   >  1:955729/1‑300 (MQ=255)                                                     tGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCAC‑TGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCt                                                                                                                                                                                                                                        <  2:242802/300‑1 (MQ=255)                                                          ttATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTaa                                                                                                                                                                                                                                   <  1:387792/301‑1 (MQ=255)                                                          ttATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTaa                                                                                                                                                                                                                                   <  1:910541/301‑1 (MQ=255)                                                          ttATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTaa                                                                                                                                                                                                                                   <  1:142706/301‑1 (MQ=255)                                                          ttATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCACGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTaa                                                                                                                                                                                                                                   <  2:113773/301‑1 (MQ=255)                                                                 gAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGGTGAAGATCACGTCAGAAAATTGTTACATTACTATGTTAc                                                                                                                                                                                                                                                          <  2:275865/271‑1 (MQ=255)                                                                 gAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGGTGAAGATCACGTCAGAAAATTGTTACATTACTATGTTAc                                                                                                                                                                                                                                                          >  1:275862/1‑271 (MQ=255)                                                                    aTTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTAGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAAGTGTTACATTACTATGTTAAGCATAACGTGATTTGCCTTGTATTTGTTATCaa                                                                                                                                                                                                                         >  1:721004/1‑300 (MQ=255)                                                                         cTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGc                                                                                                                                                                                                                                                        >  2:149918/1‑265 (MQ=255)                                                                         cTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGc                                                                                                                                                                                                                                                        <  1:149916/265‑1 (MQ=255)                                                                                                           cccGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATAATTATGCCAATCCATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCg                                                                                                                                                                                  <  1:106477/300‑1 (MQ=255)                                                                                                             cGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc                                                                                                                                                                                           >  1:230018/1‑290 (MQ=255)                                                                                                             cGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc                                                                                                                                                                                           <  2:230020/290‑1 (MQ=255)                                                                                                                            gggTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGATGCAGTTa                                                                                                                                                                 >  1:664599/1‑301 (MQ=255)                                                                                                                               ttATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAg                                                                                                                                                               <  2:664612/300‑1 (MQ=255)                                                                                                                                tATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGc                                                                                                                                                                                                                                                        <  2:713829/210‑1 (MQ=255)                                                                                                                                tATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGc                                                                                                                                                                                                                                                        >  1:713814/1‑210 (MQ=255)                                                                                                                                     gTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGAtt                                                                                                                                                         >  2:332335/1‑300 (MQ=255)                                                                                                                                                   ttattattCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACg                                                                                                                                                                                                                                                  >  1:366542/1‑197 (MQ=255)                                                                                                                                                   ttattattCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACg                                                                                                                                                                                                                                                  <  2:366546/197‑1 (MQ=255)                                                                                                                                                   ttattattCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCg                                                                                                                                                                                  <  2:559008/261‑1 (MQ=255)                                                                                                                                                                ttATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACGAGGCAGGACTGTGTTTTCACTTGCATAAATTCCTCAATGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGc                                                                                                                             <  2:955770/300‑1 (MQ=255)                                                                                                                                                                                                            acGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCATGGAGTGGTAACACATTAAGTgg                                                                                 >  1:285073/1‑301 (MQ=255)                                                                                                                                                                                                                        gtTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGt                                                                                                                                                                                                                                                             <  1:345042/117‑1 (MQ=255)                                                                                                                                                                                                                        gtTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGt                                                                                                                                                                                                                                                             >  2:345045/1‑117 (MQ=255)                                                                                                                                                                                                                                           ccTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGACGATCACGTCAGACAATTGTTACATTACTATGTTACGCATAACGTGCTGTGCCTTGTAATTCTTATCAGTATCAATAAAAAAACGTGAAATTATTATGCCGCGAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTGATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTACTAGCTGCTaaa                                                  >  1:99005/1‑301 (MQ=255)                                                                                                                                                                                                                                           ccTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTaaa                                                  >  2:177908/1‑301 (MQ=255)                                                                                                                                                                                                                                              aaCTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAAttt                                               <  1:332332/300‑1 (MQ=255)                                                                                                                                                                                                                                                  ggTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGGTGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGTTTGCCGCGTTATTATCTGCTAAAt                                                 >  1:219026/1‑295 (MQ=255)                                                                                                                                                                                                                                                  ggTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGGTGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAAt                                                 <  2:219028/295‑1 (MQ=255)                                                                                                                                                                                                                                                               ccTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTCTCTGCTAAATTTATTGATGGTGACGAt                                >  2:414152/1‑299 (MQ=255)                                                                                                                                                                                                                                                                     aaGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATcc                        <  2:721019/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTTAACTGTCATTATTTGTGATGATGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTc                                                                                                                                                                                                                                >  1:180003/1‑88 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTc                                                                                                                                                                                                                                <  2:180005/88‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAAt                                                                                    >  2:194496/1‑228 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAAt                                                                                    <  1:194494/228‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAAAAatat            <  2:955040/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTAtt                                                                                                                       >  2:926443/1‑186 (MQ=255)                                                                                                                                                                                                                                                                                         cTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTAtt                                                                                                                       <  1:926402/186‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAAAAATATAGATAAAAta  >  1:920692/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                ATGAGTCGCTATGAATAACTTTACCGATAACTTCATGACCTAACACCATCGGTGCCTTTATCATGAAATTACCTACTTTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCAAAAATATAGATAAAATG  >  NC_000913/4494178‑4494760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.