Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 209 | C→T | T7I (ACC→ATC) | thrL → | thr operon leader peptide |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 209 | 0 | C | T | 99.1% | 386.7 / NA | 115 | T7I (ACC→ATC) | thrL | thr operon leader peptide |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (61/51); total (61/54) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 116 total aligned reads displayed.
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAG > NC_000913/1‑503 | tgtccctacacgacgctcttcctatcttgcatgtaattgaaaaaaatatcaccaaaaaaaaaacgccttagtaagtatttttcAGCTTTACATTCTGACTGCAACGGTCAATATGTCTCTGTGTGGAGTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAGCTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACATACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTaccac > 2:886704‑M1/84‑301 (MQ=255) cggcaatgttgcaccgtttgctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACcaccat < 1:774050‑M1/224‑1 (MQ=255) cggcaatgttgcaccgtttgctgcatgatattgaaaaaaataccaccaaataaaacacgccttagtaagtatttttcAGCTTTTCCTTCTGACTGCAACTGGCAATATGTATCTGTGTGGTTTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGCGTAAATTTCAATTTTATTGACTTATGTCACTACATACTTTAACCACTATAGGCATAGCGCACAGCCAGATACAACTTCCAGAGTA‑CCCAACATCCATGACACGCAATAG‑CACTACCATTtacaccaccaa < 2:667625‑M1/224‑2 (MQ=255) aaatgttgcaccgtttgctgaatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGAAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACcaccatcac > 2:400079‑M1/75‑301 (MQ=255) tgcaccgtttgctgcatgatatttaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTTACTTAGGTCACTAAATACTTTAACCACTATAGGCATAGCGCACAGACAGAAAAAAATTACAGCGTA‑CACCACATCCATTAAACGCATTAG‑CACCATCATTACCACCCACATCACCATTa < 2:323846‑M1/232‑1 (MQ=255) tgcaccgtttgctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTAc > 1:901698‑M1/69‑301 (MQ=255) caccgtttgctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGGGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACACTCACCATTACca < 1:347730‑M1/235‑1 (MQ=255) ccgtttgctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGGCTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACcaca > 1:608515‑M1/65‑301 (MQ=255) ccgtttgctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACcaca > 1:324210‑M1/65‑301 (MQ=255) tgaggcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTAATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTaa > 2:322999‑M1/60‑301 (MQ=255) gctgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTaa < 1:392578‑M1/242‑1 (MQ=255) tgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAACTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATccatgaaacgcattag‑caccatcattaccacagatcggaagagcacacgtctgaact < 1:887810‑M1/244‑27 (MQ=255) tgcatgatattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACgg < 1:246469‑M1/245‑1 (MQ=255) aattgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGc > 2:773161‑M1/50‑301 (MQ=255) aattgaaaaaaatatcaacaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGc > 1:13065‑M1/50‑301 (MQ=255) tgaaaaaaatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCCTTACCACAGGTAACGGTGCGGGCTg < 2:14485‑M1/254‑1 (MQ=255) aaaaaaaataaccaaaaaaaaaaggccttagtaagtagtgttcAGCTTTACATTCGGACTGCAACGGGCAATATGTCTCGGTGTGGATTAAAAAAAGAGTGTTTGAGAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACACATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcg‑c > 2:260442‑M1/44‑301 (MQ=255) aatatcaccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTAc > 2:246219‑M1/40‑301 (MQ=255) cgatctaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAgg > 2:648289‑M1/37‑301 (MQ=255) atcaccacataaaaaacgccttagtaagtattgttcAGCATTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAgg > 2:923414‑M1/37‑301 (MQ=255) acaacaaataaaaaacgccttagtaagtaattgtcAGCTTTTCATTCTGATGGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAACAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGa > 2:895186‑M1/36‑301 (MQ=255) caccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGCCAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCACCCCAATTACCACAGGTAACGGTGCTGGCTGACG‑CGTACAGGaa < 2:715766‑M1/267‑1 (MQ=255) accaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGaaa < 2:898786‑M1/268‑1 (MQ=255) ccaaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAac > 1:955535‑M1/33‑301 (MQ=255) caaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTCCCACCACCCTCCCCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGCAaca < 2:1150137‑M1/270‑1 (MQ=255) caaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACG‑CGTACAGGAAaca < 2:788322‑M1/270‑1 (MQ=255) aaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCG‑CGTacaggagatcg < 1:649000‑M1/271‑6 (MQ=255) aaataaaaaacgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAacac > 1:462652‑M1/31‑301 (MQ=255) aaaaaaacgccttagtaagtatttttcAGCGTTTCAGTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGa > 2:429228‑M1/28‑301 (MQ=255) acgccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAg > 2:392147‑M1/22‑301 (MQ=255) ccttagtaagtatttttcAGCTTTTCATTCTGACTGCAACGGGAAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGccc > 2:90704‑M1/19‑301 (MQ=255) ccctacacgacgctactccgatcgcTGACTGCAACGGGCAATATGTCTCTGTGTTGATTAAAAAAAGAGTGTATGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACCGAGTA‑CACATCATCCATGCAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCAcc < 2:76642/277‑1 (MQ=255) taagtatttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCAcc > 1:789211‑M1/13‑300 (MQ=255) ttttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGAGTAAAAAAAGAGGGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑g > 2:545085‑M1/8‑301 (MQ=255) tttttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa‑ga < 1:545696‑M1/295‑2 (MQ=255) ttcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTAAAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACAATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCg < 1:923966‑M1/298‑1 (MQ=255) tcAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg > 1:489722‑M1/3‑301 (MQ=255) cAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGATAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCggg < 1:833486‑M1/300‑1 (MQ=255) aagtgtcaggcggACTGAAAAGGGCAAGATATGTCTATGTGGATGAAAAAGAGAGTGTCTGATAGCAGCTTCTGAAATGGTTACCGGCAGTGAGTAAATTAAAATTTTATTGAGTTAGGTGACTAAATACTGTAACGAATAAAGGCTGAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCAACACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGACAAAAGCTCGCA‑CTGTCAGGTGCGGGC‑t < 2:1137773/289‑1 (MQ=255) ttacttCTGACTGCAACGGGCAATATGTATCAGTGTGGATTAAAAAAATAGTGTCTGATAGCAGATACGGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTTACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACGTCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttttt < 2:596342/297‑1 (MQ=255) ttCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttt < 1:1088980/301‑1 (MQ=255) ttCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttttt < 1:330118/301‑1 (MQ=255) aTTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tttttttt < 2:958293/301‑1 (MQ=255) cTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCa > 1:131769/1‑301 (MQ=255) tGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACCGGAAACACAGAAAAAAGCCCGCACCTGACC‑GTCCGGGC‑TTTTTTTTTCGCCCaa > 2:627291/1‑301 (MQ=255) gCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAAAAAAAAGCCCGCACCTGACC‑GTGCGGGC‑TTTTTTTTTCGGCCaca > 2:1164366/1‑299 (MQ=255) tGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAACAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaa > 1:809242/1‑301 (MQ=255) tGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATAATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTCCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAATGGTAACGAGGTaa > 2:981810/1‑301 (MQ=255) tgtgtgGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTTGACCAAAGGTTACGAGGTaaaaacaa > 2:143483/1‑299 (MQ=255) gtgAATAAAAAAAAGGGTGTTTATTAGCAGCTTTTGAACTGTTTACTGGCCGTAATTAAATAAAAATTTTATTAATTTAGGTAACAAAATATTTTAACCAAAATAGGCATAGCGAACAAAAAAATAAAAATAAC‑GAGTACCACACCA‑CCAAGGAACCCATTTGCCACAATCTTACCCCCCCCCACCCCCATTCCCCCAGG‑AACCGGGCCGGCCTACGCCGAACAGAAACCACAGAAAAAAGCCCGAACTTAAAG‑GTGCGGGT‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg < 1:143617/300‑1 (MQ=255) tgGATTAAAAAAAGGTTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACATAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCGTTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCg < 1:716563/301‑1 (MQ=255) ttAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTTGACCAAAGGTAACGAGGTAACAAACATTCGAgtt > 1:730972/1‑300 (MQ=255) aaaaaaAGTGTCTGATAGCAGATTTTGCACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa < 2:832502/301‑1 (MQ=255) aaaGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg < 1:1056372/301‑1 (MQ=255) gcagcTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACCTCa > 1:1123147/1‑301 (MQ=255) tCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa > 1:162237/1‑301 (MQ=255) gAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATTCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAAt > 2:922604/1‑301 (MQ=255) ttcTGGTTACCTGCCGTAAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg < 2:972698/299‑1 (MQ=255) cttccgatcTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGAACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 2:934376/293‑1 (MQ=255) ggTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATgagat > 1:973897/1‑297 (MQ=255) tttaactacattaaatAAAGTAAAATTTTATTGACTTAGGGCACAATATACTTTAACCAATGTTGGCAAAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACAGCCATGAAAACCATTAG‑CACCATCATTACCCCCACCTTGCCCATTCCCACAGGGACCGGTGCTGGCTGACG‑CGTACAGGAAAAACAGAAAAAAGCCCGCACCTGACA‑GTGAGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa < 2:1165333/286‑1 (MQ=255) gTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCAGGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGAC‑TTTTTTTTTCGACCAAAGGTACCGAGGTAACAACAACGCGAGTGTTGAAGTTCGGCGGTACCTCAGCGGCAAATGCAGaa > 1:1166794/1‑301 (MQ=255) gCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCaagatcggaaa > 1:935529/1‑291 (MQ=255) tGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGc < 1:830197/300‑1 (MQ=255) tAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 2:131635/301‑1 (MQ=255) aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGAGGTAAATCCGTGGCAAATGCAGAACGTTTTCTGCGTGTTTCCGaa > 2:295696/1‑300 (MQ=255) ttttATTGACTTAGGACCCTAAATACTTTAACCAATATAGGCAAAGCGCACAGACAGATAAAAATTAAAGAGTA‑CACAACATCCATGAAGCGCATTAG‑CACCATCATTACCCCCACCTTCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAACCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCTGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc < 1:922909/301‑1 (MQ=255) tATTGAATTAGGTAAATAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAACTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 2:893796/301‑1 (MQ=255) tctGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa < 1:690008/299‑1 (MQ=255) gACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCCAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaga > 2:689259/1‑299 (MQ=255) tAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATg > 1:371345/1‑301 (MQ=255) caacgctcttccgatctAACCAATATAGGCATAGCACACAGACAGATAAAAATTACAGAGTA‑AACAACATCCATGAAACGCAATAG‑CACCATCATTACCACCACCATCACCAGTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATg < 2:987279/285‑1 (MQ=255) tCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCCGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAACGCAATGcc > 2:655786/1‑300 (MQ=255) cACTAAATACTTTAACCAATATAGGCAAAGCGCACAGACAGATAAAAATTACCGAGGA‑CACAACATCCACGAAACGCATTAG‑CACCCTCATTACCACCCCCATCACCATTACCACAGGTAACGGCGAGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcag < 2:719309/301‑1 (MQ=255) aaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGAAATTCTGGAAAGCAATGCcaggcagg > 1:708893/1‑301 (MQ=255) aaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACATCAGTGGCCAATTCAGAACGTTTTTTGCGTGTTGCCGGTATTCTGGAAAGACAGGCcagggagg > 2:410588/1‑301 (MQ=255) aCTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCTACCAAAGGTAACGAGCTAACAACCATGCGATTGTTGAAGTTCGGCGGTCCATCAGTAGCAAATGCAGGACGTTTTTTTCATGTTGCCGATATTCTGGACAGCAATGCcaggccggtg > 2:745387/1‑298 (MQ=255) ttAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAATA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg < 1:1001143/301‑1 (MQ=255) aaCCAATATAGGCATAGCACACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAAGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGAAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATgagttcggaagagcaca > 1:988531/1‑284 (MQ=255) ccAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTTCCGATCTTCTTGAAAGCACTGCCATGCATGGGCAGGttg > 2:811097/1‑298 (MQ=255) ccAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGATAACAACCATGCGAGTGTTTAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGACAGGAAGGGGAAGGTggg > 1:472197/1‑300 (MQ=255) ccAATATAGGCATAGCACACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGc < 2:646945/301‑1 (MQ=255) aaTATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCa > 1:1062244/1‑301 (MQ=255) aaGCGAACAGACAGATAAAAATTCAAGAGTA‑CACAAGAGCCATGAAAGGCAATAG‑CACCATCATTACCTCCACCATCACCATTACCACAGGTAACGGTGCGGGCGGACG‑CGTACAGGAAACTCAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCTTGCGAGTGTTGAAGTTCGGCGGTACATCAGTTGCAAATGCAGAACGTTTTCTTCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCctctc < 1:668374/300‑1 (MQ=255) cgaacagacaaAAAAAAATAACAGAGTA‑AACAACAACCATGAAACGCACTAG‑AACCAGCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGGCG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTg < 2:18357/297‑1 (MQ=255) aagaTAAAAATTACAGATTA‑CACATAATCCATGAAACGCATTAG‑CACCATCATTACCACCACCACACCCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCa < 1:746232/300‑1 (MQ=255) aTAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCg < 1:863614/295‑1 (MQ=255) aTAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCg < 1:14486/295‑1 (MQ=255) aTAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCaaaa > 1:823848/1‑301 (MQ=255) tAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGCGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAATGCAATGCCAGGCAGTGGCCGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAAt > 2:1013202/1‑301 (MQ=255) aaaTTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAACGGGAACGAGGTAACAACCATTCGCATGTTGAAGCTCGGACGTCCATCAGTGGCAACTGCAGAACGTTTTCTGCGTCTTGCCGATATTCTGGAAAGCAACACCCGGCAGCGGCAGGCGGCCTCCCGTCGCTCAGcccccccacaaaaca > 2:427856/1‑290 (MQ=255) tACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCaccaac < 1:899892/301‑1 (MQ=255) tA‑CACAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCCCCAACATCCCCCACCACCtgg > 2:45320/1‑301 (MQ=255) a‑cacAACATCCATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGTGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCtggt > 2:483448/1‑301 (MQ=255) aaccTCCATGAAACGCATTAG‑AACCATCATTACCACCACCAGCACCATTAACACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCg < 2:553901/301‑1 (MQ=255) cATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCGGAACGTTCTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCgatgatc < 1:1108378/301‑2 (MQ=255) cATGAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGAtt < 1:627964/301‑1 (MQ=255) gAAACGCATTAG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCTAGTATTGAAGTTCGGCGGTACATCCGTGGAACATGCAGAAAGTTTTCTGCGTGTTTCCGTTATTATGGGAAGAAATGCCAGGCAGGGGCAGGTGGCCACAGTCCTTCCTGCCCCCCCAAAAATCACCAACCACCTGGTGGCGttgattga > 2:478164/1‑301 (MQ=255) aG‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGCTGATTGACAAAACCATTa > 1:896351/1‑301 (MQ=255) g‑CACCATCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACAAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTaa > 1:104868/1‑300 (MQ=255) | ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAGTGTCTGATAGCAGCTTCTGAACTGGTTACCTGCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTA‑CACAACATCCATGAAACGCATTAG‑CACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACG‑CGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAG > NC_000913/1‑503 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |