Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,427,383 A→C 100% noncoding (1380/1542 nt) rrsD ← 16S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,427,3830AC27.5% ‑6.7 / 2.5 4noncoding (1380/1542 nt)rrsD16S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (2/0);  total (2/2)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01
Rejected as polymorphism: Not supported by required number of reads on both strands.

ACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGC  >  NC_000913/3427092‑3427447
                                                                                                                                                                                                                                                                                                   |                                                                
acgtGAACCGCCGACCTCACCCTTATCAGTGCTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCGTCTGTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCAc                                                         <  2:82303/298‑1 (MQ=0)
 tttgacacaccGACCTCAGCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGGAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCAcc                                                        <  1:834445/294‑1 (MQ=1)
                                                 aGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGACTCACAACGTGGTACGCGCCCTCCCGAAGGTTAAGCTCCCTACTTCTTTTCCACCCAACTCCCATGTTTTCACGGCCGGTGTTTCCAAGGCCCGGGACCTTCTCCCCCGTGGCTTTCTGACCCCCGTTTCCtcacttttcctcattctgggagtca        >  1:1485332/1‑277 (MQ=1)
                                                        aGCCTGTCGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGCTCCCACCGCAGGTTCCCCTACCTTTCCCTTCTTCCGACTTCACCCCAGTCATGCCTACCAAAGTGGTAAGCGCACTACCAACGGTTCAGCTACCTACTTCTTTTGCAACCCCCTCACCTTGCTTGCCGCGAGGTCTGTACAAGGCCAGGGCCCGTATTCACCCGGGCTATAGGACCCACGactactgcagcttcaacactcttggatcatattgtc  >  1:1325252/1‑270 (MQ=2)
                                                                                                                                                                                                                                                                                                   |                                                                
ACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGC  >  NC_000913/3427092‑3427447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑