Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,427,3230A→C100.0% 1.6 3noncoding (1440/1542 nt)rrsD16S ribosomal RNA of rrnD operon
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (0/0):  total (3/0)

AATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCA‑ACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTAT‑TCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATC  >  NC_000913/3427072‑3427458
                                                                                                                                                                                                                                                           |                                                                                                                                         
aaTTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTTCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCAAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCAGACGTTTAGGCACCCTAATTCTTTTGCA‑ACCACCTCCACTGGGTTCCCGGGCGttgtgataa                                                                                         >  1:365534/1‑297 (MQ=11)
                                       cccTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTAATCAGACAATCTGTGTGAGCACTTTAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTACCCTACGGTTACCTTGTTACGAATTCACCCCAGTCATGACTCCCACAGTGGTAAGCTCCCTCCCGACGGTTACGCCCACTACTGCTTATGCACACCCA‑TCCCCTGGTTGGAACGGCGATCTGTACCAGGTCCGTGCACCTATGTCCCCGT‑GCATTcgtgcccaca                                                 >  1:766579/1‑292 (MQ=11)
                                                                                       gTTTTACTGCACATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGATTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTACCCTACGGTTACCTTGTTACGACTTCACACCAGTCATGCATCTCAAAGTGGTGAGCGCCCTCCAGCAGGGTCAGCTGCGTACTCCTTTTGCA‑AACCACCACCACGGCGTGAAGAGAGGAGTGTCCTAGGCCCGGGCACGTAT‑CCACGTTCACATTCTGATCCCCGATTACTATCTATTCTGCCCTCCTGGatgagacttgcacaccaccatc  >  1:767058/1‑279 (MQ=0)
                                                                                                                                                                                                                                                           |                                                                                                                                         
AATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTTCATCAGACAATCTGTGTGAGCACTGCAAAGTACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCA‑ACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTAT‑TCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATC  >  NC_000913/3427072‑3427458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.