Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,407	C→A	M272I (ATG→ATT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,407	0	C	A	100.0%	132.0 / NA	38	M272I (ATG→ATT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/20);  total (18/20)

CGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATT  >  NC_000913/4116114‑4116704                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                          cGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAagc                                                                                                                                                                                                                                                                                                    <  2:50686/301‑1 (MQ=255)         aCCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTg                                                                                                                                                                                                                                                                                            >  2:383792/1‑301 (MQ=255)            gAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCGCGCCAGTGTTCATCAGAATAAATCAGCCAGTGcc                                                                                                                                                                                                                                                                                          >  1:158905/1‑300 (MQ=255)                   cccccGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGgtgt                                                                                                                                                                                                                                                                                  <  2:159071/300‑1 (MQ=255)                           cgcATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGc                                                                                                                                                                                                                                                                           <  1:423058/300‑1 (MQ=255)                                 cGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATcc                                                                                                                                                                                                                                                                      >  2:299012/1‑299 (MQ=255)                                                    cccAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCTCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGa                                                                                                                                                                                                                                                  >  2:425147/1‑300 (MQ=255)                                                    cccAGACCGTGAAAAGCCGGAACCACTTACACACCAGGGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATGGTAGGCGACGTTAATCAACTTCATTGCATCGCGCAGCCACTGAATGGATGCGCCTTCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGAc                                                                                                                                                                                                                                                 <  1:298668/301‑1 (MQ=255)                                                             gTAAGTGCCGGAACCACATACACGCTCTTGGGGTTTTGCCTTGTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCACATTCATCTCATCGCGCAGCCACTGAAGGGATGCGCCTGCCATAAACCCCGCACCTTCCAACGCATGGGTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGGTTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGttct                                                                                                                                                                                                                                                                               <  1:118363/262‑1 (MQ=255)                                                                   gCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGcc                                                                                                                                                                                                                                   >  2:377609/1‑300 (MQ=255)                                                                    ccGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCct                                                                                                                                                                                                                                  <  2:61464/300‑1 (MQ=255)                                                                       gAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAAGATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCctgct                                                                                                                                                                                                                               <  1:406288/300‑1 (MQ=255)                                                                          ccACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTc                                                                                                                                                                                                                           <  2:8833/301‑1 (MQ=255)                                                                                           gtgtTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTACTCACCTTCAGTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGgag                                                                                                                                                                                                           <  1:377162/300‑1 (MQ=255)                                                                                           gtgtTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCCCCGGCGATCCCGgaga                                                                                                                                                                                                          >  2:257565/1‑301 (MQ=255)                                                                                                                  ttgctcttccgatctggCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTg                                                                                                                                                                                   <  2:262225/286‑1 (MQ=255)                                                                                                                                 ggCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTgcgttcggaagagggg                                                                                                                                                                    >  1:261930/1‑287 (MQ=255)                                                                                                                                                    atttcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGAACCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaag                                                                                                                                                 >  2:129343/1‑301 (MQ=255)                                                                                                                                                       tcatCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAac                                                                                                                                              <  2:149993/301‑1 (MQ=255)                                                                                                                                                                              tGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGTAAGAACGACGCACTTCTGGCAGCAtctc                                                                                                                        >  1:193704/1‑300 (MQ=255)                                                                                                                                                                                cgcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgcg                                                                                                                     >  2:102629/1‑301 (MQ=255)                                                                                                                                                                                                    cacgtcgctctaccgagctcTTCGCCAGTCGGGTCGAAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTAACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCa                                                                                                 <  1:183224/282‑1 (MQ=255)                                                                                                                                                                                                         cgtggtctcttccgatctgCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                                                                            <  2:47041/283‑1 (MQ=255)                                                                                                                                                                                                                      cggcgctcttccggtctgCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGt                                                                               <  1:351747/284‑1 (MQ=255)                                                                                                                                                                                                                       cTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCCAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAACACTTCCaagatcggaagatcacacgt                                                                              >  2:183419/1‑282 (MQ=255)                                                                                                                                                                                                                           gCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGCATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCTTCTCGCGCGGAATATCCCGCACTTCCAGCAttcgatcggaagagcgtcgg                                                                          >  1:46989/1‑283 (MQ=255)                                                                                                                                                                                                                                 cGGGCCGCAGGGGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATg                                                                     <  1:383330/300‑1 (MQ=255)                                                                                                                                                                                                                                       gCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGtagatcggaagagcaca                                                               >  2:352161/1‑284 (MQ=255)                                                                                                                                                                                                                                          ggCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGACTACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGACCGACGCACTTCTGGCAGCATCTCGCGCGGAATCTCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa                                                            >  1:364292/1‑300 (MQ=255)                                                                                                                                                                                                                                          agggATGGTGGTCAGCAGTCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGa                                                             <  1:257287/296‑1 (MQ=255)                                                                                                                                                                                                                                                          tccacgccgctcttccgatctgCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAgg                                           <  1:267851/280‑1 (MQ=255)                                                                                                                                                                                                                                                            gCCGTTGTCTGATGTCGCCGATTTCGCGCCAGTGTTCATCATAATAAAGCGGCCAGTGCCATAGGTGTCCTTCGCCAGCCCTTCTTTCACTCACAACTGACCGACCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCAAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGAATCTCGCGCTGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAgg                                           <  1:424627/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  ttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCCATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGGATTGATGTTGAACAACATGTTACGAGAGGCGTTGgtg                                   >  1:422278/1‑301 (MQ=255)                                                                                                                                                                                                                                                                      gATTTCACCGCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTTACCGTCTACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGTCACGAGAGCCGTTGgttaaat                               >  1:418805/1‑296 (MQ=255)                                                                                                                                                                                                                                                                               gCTTTCTCGCCAGTGTTCATCAGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAggagatcggaagagcacacgt                        >  2:268146/1‑280 (MQ=255)                                                                                                                                                                                                                                                                                     ttgCCAGTGTTCAGCAGAATAAAGCAGCCAGTGCCATATGTGTTCTTGGCCATCCCTCATTTCACGCACACCTGAACAAACAGCGCGGCGTTCTGGTCACCGGCGATCCCTGAGATTTGAATACGCGTGCCGCCTTTTCCGCAAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGacac                <  1:129212/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tcatcaGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGt      <  2:216827/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   caGAATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCCATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGACGAACTACGCACTTCTGGCAGCATCCCGCGCGGAATATACAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGTTGTTGAACAACATGGTACGAGAGGAGTTGGTGTAGTCGGTCACATGGACAGGGCTCTGAGGCAtt  >  1:397791/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                          CGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATT  >  NC_000913/4116114‑4116704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑