Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,560,113	A→C	12.1%	pseudogene (341/606 nt)	glpR ←	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,560,113	0	A→C	12.1%	5.5 ‑0.3 0.0	57	pseudogene (341/606 nt)	glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (26/21):  total (33/24)
Fisher's exact test strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

GTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGT  >  NC_000913/3559830‑3560404                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    gTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATAAAGGCGGCACTGGGAGATa                                                                                                                                                                                                                                                                                    >  2:363699/1‑301 (MQ=255)                ggATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCATTTGGTGAGAACTTCGTGGTAATCGAACTCCATCAGCCAGCAGTCGCTATAGATGCCCCTTATCCCCAGAAGGCCGAAATCCAGGCGGAACTTGAGGTTGAAATCGatgcgtg                                                                                                                                                                                                                                                                       >  2:433336/1‑293 (MQ=255)                 gATCAGCACAGCGCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGGTTACTGGCGGCAGGGCGTCGGTGTAGAAGGCATCTACCATGCTGATGCTGCCAATATTGACCATAGCGTTACGGCCGAATTTCGAGTGATCGACAACCAGCATAACCTGGCCCGAGTTCGCAATCATGGCGAGATTGGTGCGAACTGAGGGATAATGTAACCCCAGCCACGAGGCGGCGCTGTCGCGTCCGCTTCTCACCAGAAGACCGAAATCAACGCGGCACTTGTGGATAAACTCTAGCGGCGCtt                                                                                                                                                                                                                                                                    >  2:433737/1‑301 (MQ=255)                      gCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGCGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCACCACACTGCCGAACTCACGGAGGAACTGGGAGATCAAATCAACCGTAGCTTCGCCa                                                                                                                                                                                                                                                               >  2:241803/1‑301 (MQ=255)                        acggCTCCGTTTGAAGAGGGTGGTCCGTCAGCACCGGCATCACGCTTACGGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGCTGCTGCCCATATTGACC‑TCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGTGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATg                                                                                                                                                                                                                                                            <  1:302723/298‑1 (MQ=255)                            ccccAGTTGAAGATGGTGGTCCGTCAGCACCTGCAGCACTCTTACTGGCGTCGGGGCGGCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATc                                                                                                                                                                                                                                                         <  2:378698/299‑1 (MQ=255)                                aGTTGAATATGGTGGTCCGTCAGAACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGTGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGCACTTCGTGGTAATCGAACTCCATCAGCGAGCCGTCTCTATCGATGACGCTTATCTCCACAATGACGAAATCAGGGCGGAATTGTGAGATACATTCTACCGTCGGTCCGCCTATGacgccg                                                                                                                                                                                                                                                      >  2:200301/1‑295 (MQ=255)                                                  gTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCCTAACGTGGCGCGCGTTCTCAATCATGGCGCGTTTGGTGCGACCTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCAACCATGCCTAAATCACGGCGGAACTGGGAGATCACATCGAGCGCCGCCTCGCCAATTATCCCCCCCTCGCGCCtcgctaatt                                                                                                                                                                                                                                   >  1:351625/1‑293 (MQ=255)                                                      gCACCTGCATCACGCTTACTGGCGGCGGAGCGTCGGTGTCGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTTGTAATCCAACTCCATCCGCCAGCCTTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGCACTGGGAGATAAAATCGAGCGTCGCTTCGCCATTGTTCCCCCCCTCCCGGCtcacaagttccc                                                                                                                                                                                                                                >  1:317884/1‑289 (MQ=255)                                                            gCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAAGTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCATGGTCATCGAACTCCAGCAGCGAGACGTCGCTATCGAAGCTGCTTATACCCAGACTGCCGAACTCAAGGCGGAACTACGCGAAAAAATAGACAGCCGCTTCGACACGAATAAAGCAATCGCGGCTGAGTAATACGCCACCgg                                                                                                                                                                                                                          >  2:386200/1‑300 (MQ=255)                                                             cATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCCATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCCCCATCGCGGCTGCGTAATTCGCCCCCTGCg                                                                                                                                                                                                                        >  1:342420/1‑301 (MQ=255)                                                                  cGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGACCTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCCTCTCCCCCGACTGCCGAAATCACGGCGGAACTGGGAGATCAAATCGAGCGTCGCTTCACCAATGCTCCCGCCCTCGCGGCTCCCTCATTCCCCACCGCCCAGaaa                                                                                                                                                                                                                   >  1:326380/1‑300 (MQ=255)                                                                                         gtgtAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGCGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGCCTGCGTAATTCGCCACCGGCGAGAATGATGCGCAACTCCTCTTTTCcc                                                                                                                                                                                             >  1:142914/1‑300 (MQ=255)                                                                                         gtgtAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCTTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCCCCGCCGCGAATGATGCGAAAATCCTCTTTTAcc                                                                                                                                                                                             >  1:206586/1‑300 (MQ=255)                                                                                         gtgtAGACGGCATCTACCATGCTGATGCTGCCCATATTGAACATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTACTCGAACTCCAGCCGCGAGCCGTAGCTATCGATGCCGCTTATCCCCAGCATTCCGAACTCAAGGCGGAACTTTGAGATCAAACCGTGGTTCGCTTCGCCAATGATCACGCCCACGCGGCTTACTAATTATACCCCGGAGAGAACGACGCgctaacactctttttca                                                                                                                                                                                             >  2:112903/1‑284 (MQ=255)                                                                                                  gCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCAGCTTATCCACAGAATGACGAACTCAAGGCGGAACTGGGAGGTAAAATAGAGCGGCGCTTCGCCACTGATCCCACCATCGCGGCTGCGTAATTGGCCACCGGCGAGAATGATGCGAAACTCTTCTTTTACCAtaacagtgt                                                                                                                                                                                   >  2:162959/1‑293 (MQ=255)                                                                                                     ccgaCCAGGGTGATGCGGCACAGATTGACCATGGGGTTACGGCCAAATTTCGAGTGACCGACAACCAGCAAAACGTGGGGCGAGTTCTCAATAATGGCGGGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAg                                                                                                                                                                                <  2:342429/298‑1 (MQ=255)                                                                                                      cTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATCTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATTCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGCTCAAATCGACCAGCGCTCCGCCAATTATCCCGCCATCCCCGCTGCGTCATGTCCCACCGGCCACAATCATCCGCAATTCTCCTTTTACCAtacaacttttcga                                                                                                                                                                               >  1:130521/1‑289 (MQ=255)                                                                                                        aCCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCGTAACGTGGCGCGAGGACTCGATAATGGCGCGGTTGGTGCGAACGTAGTGGTGATCGAACGCCAGCAGAGAACCGGCGCTATCGATGCCCGTTATCCCCAGAATGCCGAAATCAAGGCTGAACTGGGAGCTAAAATCGAGCGTCGCTTCGCCAATCGTCCCGACATACAGGCTGGGTAATTCGCCACCTGCGATGATGATGGGCAAATCTGCTTTTACCATCAACGTGTTAGCaa                                                                                                                                                                             >  2:71775/1‑301 (MQ=255)                                                                                                        aCCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGGGCGAACTTAGTGGTAAT‑‑‑‑‑TCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATACCCAGAATGCCGAAATCCAGGCGGAACGGGGAGATAAAATCGAGCGGCGCTTCGCCAATGATCCCGCACTCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCACCGTGTTCGCAACGTTg                                                                                                                                                                        >  2:142920/1‑301 (MQ=255)                                                                                                         ccATGCTGATGCGGCTCATATTGAGCAGTGAGTTAACGCCAAAGTGCGAGGTGTCGACAACGATCAGAACGTGGAGCGAGTGCTCAATAATGGCGAGTTTGGTGCGAACTTCGTGGTAATCGAACTACAGCAGCGAGCCGTCGCGATCGATGCCGCTTATCCCCAGAATGCCGAAATCACGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAAc                                                                                                                                                                            <  2:326388/301‑1 (MQ=255)                                                                                                           atgctgatgctgCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTACTTCGCCACCGGCGAGGATGATGCGAAAATCTTCTTTTACCATCAACGTTTTAGCAAAGt                                                                                                                                                                          >  2:322532/1‑301 (MQ=255)                                                                                                            tgctgatgctgCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAAGCGAACTCCAGCAGCGAG‑‑GTCGCTATCGATGCCGCTTATCCCCAGACTGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGATCGTCGCTTCGCCAATGCTCCCCCCCTCGCGGCAGCGTAATTCGCCACAGGCGCACATGAAGCAACAATCTTCTTTTACCATACACGTGTTAGCACCGTTgg                                                                                                                                                                       >  2:414352/1‑300 (MQ=255)                                                                                                                tgtgacacacagactGACCAACGCTTTGCTGCTAAATTTCGAGTGATCGATAATGAGCATAACGTCGCGAGAGTTCTCAATAAGCGCGCGTTTGGTGCGAATTTAGTAGTAATCGAACACGAGCAGAGGGGCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGACCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGACAATCTTCTTTTACCATCAACGTGTTAGCAACGTTgaga                                                                                                                                                                     <  1:71775/287‑1 (MQ=255)                                                                                                                 atgctgCCCATATTGACCATCGCGTTACGGCCAAATTTATAGTGATCGACAACCAGCATAACTTGGCGCGAGTTCTCAATAATGGCGCGTTTGTGGCGAACTTCGTGGTAATCGAACTACAGCAGCGAGCCGTCGCTATCTATGCCGCGTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGAt                                                                                                                                                                    <  2:206589/301‑1 (MQ=255)                                                                                                                         cATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTCTCCCCAGAATGCCGAAATCAACGCGGAACTGGGAGATACAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCATAATTCGCCACCCGCCACACTGGTTCGCCAACCGTCTTTTCCCCTCCACCTGTTACCCACGTTTACTTTGTTGGTg                                                                                                                                                            >  1:390331/1‑301 (MQ=255)                                                                                                                          atatTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGACCTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCACAGAATGCCGAAATCACGGCGGAACTGGGAGATAAAAGCGAGCGTCGCTTCTCCAATCATCCCGACATAGCGGCTTCGTAATTCGCCACCGGCGAGACTGCTGCGAAAATCTTCTTTTACCATCCACGTGTTAGCAACGTTgacagctgtctttt                                                                                                                                                           >  2:357863/1‑289 (MQ=255)                                                                                                                          atatTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTTGCTATCGATGCCGCTTATCCCCAGACTGCCGAAATCACGGCGGAACTGGGAGCTAAAATCGAGCGTCGCTCCGCCAATTATCCCGCCATCGCCGCTTCTGAATTCCCCCCGGGCGAGAATGCTCCGAAAATCTTCTTTTACCCTCAACGTGTTAGGAACGTGgagcgtgtggggga                                                                                                                                                           >  1:223974/1‑290 (MQ=255)                                                                                                                          atatTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCACGGCGGAACTGGGAGATACAATCGAGCGTCGCTTCCCCAATTATCCCGCCACCGCGGCTGCGTAATTCGCCACCGGCGAGAATGCTGCGAAAATCTCCTTTTTCCCTCAACGTGTTCGCAACGTTTAGCTTGTTGGtta                                                                                                                                                           >  2:20542/1‑299 (MQ=255)                                                                                                                          atatTGAACATCGCGTTACGGCCAAATGTGGAGTGATCGATAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCACGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGAt                                                                                                                                                                    <  1:357854/292‑1 (MQ=255)                                                                                                                                   ctCGCGTGACAGCCAAATTTCGAGTGAACGACGACCAGCAGAACGAGGCGCGAGTGCACAATAATGGCGTGTTTGGTGCTAACTTCTCGGTAATCGAACGCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATTCCCAGACTGCCGAAATCAAGGCGGAACTGGGATATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCa                                                                                                                                                  <  2:317892/300‑1 (MQ=255)                                                                                                                                              tccAAATTGCGAGTGAGCGACAACCAGCATAACGTGGAGCGAGTTCTCAATAATGGCGCGGTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCATCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACgtg                                                                                                                                                                                    <  2:411137/255‑1 (MQ=255)                                                                                                                                              gCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATCCGAAAATCTTCTTTTACCATCAACgtg                                                                                                                                                                                    >  1:411128/1‑256 (MQ=255)                                                                                                                                                cAAAATTGGAGTGATCGACAACCAGCATAACGTGGGGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCTGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCAAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGAtt                                                                                                                                     <  1:386191/301‑1 (MQ=255)                                                                                                                                                  aaTTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCCAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTTTTGGTGACAATGCGCAAATTGCTGTGATTGa                                                                                                                                   >  1:408364/1‑301 (MQ=255)                                                                                                                                                    tttCGAGGTACCGACAACCCGACAAACGGGGCGCGAGTTCTCAAGAATGGGGCGGTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCCGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGc                                                                                                                                 <  1:200299/301‑1 (MQ=255)                                                                                                                                                    ttgcggggtatctagaagcAGCATAAAGTGGCGGGAGTTGTCAATACGGGCGGGTTGGGGGCGAACTGTGTGGGAATCGACCTCCAGAAGCGCGCCGGCGCTATCGAGGACGCTTATCCGCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTGGCTGCGCCAATGATCCCGCCATCGCGGCGGCGTAATTCGCCACTGGCGAGAATGATGCGAAAATCTTCTTCTACCATCAACGTGTTAGCAACGTTGAGATTGGTGGTGACAATGCGCAAATTGCTGTGATTGAGc                                                                                                                                 <  1:235021/283‑1 (MQ=255)                                                                                                                                                      ttgAGTGATCAACAACCAGAATGAAGGGGCGCGAGTTCTCAATAATGGCGTGTGTGGTGCGAACTTCGTGGTAATCGAACTCCAGAAGCGAGCCGTTGCTATCGATGACGATTAACCCCAGAATGCCGAAAGAGAGGCGGAACTGGGAGATAAAAGCGAGCGTCTCGTCGCAAATGAGCGCGCCATGGCGGCTGAGTAATTCGCCAACGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGGGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAg                                                                                                                               <  2:351634/299‑1 (MQ=255)                                                                                                                                                         aGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATACAATCGAGCGTCGCTTCGCCAATGCTCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCCACGATGGCTCGACAAGCTTCTTTTACCCTCCACGTGTTAGCAACGATGAGCGTGTTGTTGACAATGCGCAAATTGACGTAATTgtggattg                                                                                                                             >  2:399711/1‑293 (MQ=255)                                                                                                                                                                  cAACCAGCGTAACGGTGCGCGCGTTTTGAATAATGGTGCGTTTGGTGCGAAGTTCGTGGTAATCGAACTCCAGCAACGAGCCGTCGCTATCGATGCCGCGTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGACAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAc                                                                                                                   <  1:112903/301‑1 (MQ=255)                                                                                                                                                                   aaCCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGGTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGAGCtttg                                                                                                                  >  2:349583/1‑298 (MQ=255)                                                                                                                                                                   aaCCAGCATAACGGGGCTCGAGTTCGCAAAAGTGGCGCGGTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGtgcgtgcg                                                                                                                       <  1:349574/296‑1 (MQ=255)                                                                                                                                                                                        gTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTCGCAACGTTGCGATTGTTGGTTGAAATGCGCAAATTGCTGTGATTGAGCCGTCCGTGCGCTACCGCTTCCGGCGTGGGTCCGata                                                                                             >  2:198952/1‑301 (MQ=255)                                                                                                                                                                                         tgctcAATAATGAGGCGTTTGTTGCCATCTTCGTGGTCACCGAACTCCAGCAGCGAGCCTGCGATATCGATTCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGTACATACAATCGAGCGTCGCTTCCCCAATGCTTCCGCCATCGCGGCTGCGTGATTCGCCAACGGCGAGAATGATGCGAACATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGATAATGCGCAAATTGCTGTGATTGATCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGata                                                                                             <  2:142915/298‑1 (MQ=255)                                                                                                                                                                                             cAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGCTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGAGATCGAt                                                                                        >  2:344202/1‑301 (MQ=255)                                                                                                                                                                                                 aatGGGTCGTGTGGCGCGAACTTCGTGGTAATCGAACTCCCGCAGCTAGCCGTAGCTATCGATGCCGCTTATCCCCAGAATGACGAAATCAAGGCGGAACTGTGAGATAAAAGCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGAGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCAGATATCGATAAAc                                                                                    <  2:300394/301‑1 (MQ=255)                                                                                                                                                                                                 aagggCGCGGTTGGTGCGAACTGCGTGGTAATCGAACTCCAGCAGCGAGCGGGCGCTATCGATGCCGCTTAGCCCCAGAATGACGAAATCAAGGCGGAACGGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAAc                                                                                    <  1:322524/298‑1 (MQ=255)                                                                                                                                                                                                 aaagggggggttGGGGCGAACTTCGTTGTAACCGAACGCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAAc                                                                                    <  1:363690/291‑1 (MQ=255)                                                                                                                                                                                                          tttGGTGCGAACTTCGTGGGAATCGAAGCCCAGAAGGGAG‑‑GTAGCGATGGATGCCTCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGAGAAAATCGAGCGGCGCTTCGCCAATGAGCCCGCCATCGCGGCGTCGGAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGc                                                                          <  1:414343/300‑1 (MQ=255)                                                                                                                                                                                                             ggTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAG‑‑GTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAAGTCGCAAATTGCTGTGGTTGAGCAGTGCGTGCGCTACCGCTTACGGCGGGCTGCCGCTATCGAGAAACAGCGTAGCACCAtt                                                                      >  2:217357/1‑301 (MQ=255)                                                                                                                                                                                                                          tgttaaccacactacggcatcGAGCCGTCTCTATCGATGCAGCTTATCCGCAGAATGCCGAAATCACGGCGGAACTGGGAGATAAAAGCGAGCGTCGCTGCGCAAATGCGCCCGTCATCGCGGCTGCGTAATTCGCCACCGGCGAGAAGGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTTTTGGTGACAATGCGCAAAGTGCTGTTATTGAGCAGTGCGTGCGATACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGc                                                            <  2:327467/284‑1 (MQ=255)                                                                                                                                                                                                                          tGGCAAACGAACACCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGc                                                            <  1:379027/300‑1 (MQ=255)                                                                                                                                                                                                                                   aaCTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTTCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACtt                                                  >  2:261851/1‑301 (MQ=255)                                                                                                                                                                                                                                     atcaagctgcGAGCCGTCGCTATCGATGCCGCCAAGCCCCAGAAGGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGATGGTCGCTTCGCAAATGATCCCGCCATCGCGGATGCGTAATTCGCCACCGGCGAGAATGATGCGACAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCTAGCCATTGGGGATTTGCTCCGCCACTTTg                                                <  2:408373/297‑1 (MQ=255)                                                                                                                                                                                                                                                           acGAAGCCGCTGAGCGCCAGAAGGCCGAAAGAAAGGCGGAACTGGGGGATAAAATCGGGCGTTGCATCGCTGATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGGATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCTAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTttctt                          <  2:130522/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTTACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGCTAAACAGCGTCTTGCCATTGCGGATTTGCTCCGCCACTTTGCGTGCGATGCGCTCTTTTTCTTCGGTCTGGGTgg              >  2:73068/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ccGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAACGCTTCCGGCGTGGTGCCGATATCGCTCAACAGCGTCTCGCCCTTGGGGATTTGCTCCGCCACTTTGCGGTCGATGCGATCTTTTTCTTCGGTCTGGGTGGCCTTGTGATCGt  >  2:66576/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    GTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGT  >  NC_000913/3559830‑3560404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.