Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,994,054	A→T	6.8%	pseudogene (116/444 nt)	pbl →	pseudogene, peptidoglycan‑binding enzyme family

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,994,054	0	A→T	6.8%	3.2 0.0 ‑0.0	59	pseudogene (116/444 nt)	pbl	pseudogene, peptidoglycan‑binding enzyme family
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (24/29):  total (27/32)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.36e-01

ATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCA  >  NC_000913/2993758‑2994343                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                  aTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCATCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCa                                                                                                                                                                                                                                                                                               >  1:528311/1‑301 (MQ=255)                                     tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAACaaa                                                                                                                                                                                                                                                           >  2:817036/1‑300 (MQ=255)                                              gATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTATTTCGCCAGAAACTCTTACTCTAATGAAGCAGGAAAAATGTTCAGAATCGAACCAAATTTAATTAAAGCTAGCGATAGTGTTGAAAGCAATCTaaaaaaa                                                                                                                                                                                                                                                  >  1:519938/1‑300 (MQ=255)                                              aatatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCGTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAg                                                                                                                                                                                                                                                  <  2:907533/300‑1 (MQ=255)                                               ttatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAgg                                                                                                                                                                                                                                                 <  2:518033/300‑1 (MQ=255)                                               atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATGTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAgg                                                                                                                                                                                                                                                 <  2:699824/301‑1 (MQ=255)                                               ataaGTATTCAGAAGATAATCCGAGCTCAACTTACTATAAAATTGAGTAGTAGACGATTGAACAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTGTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTGTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACGCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAgg                                                                                                                                                                                                                                                 <  1:1102769/301‑1 (MQ=255)                                                          aaagaTAACCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACTAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAGATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGc                                                                                                                                                                                                                                      <  1:1042673/300‑1 (MQ=255)                                                            agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCaa                                                                                                                                                                                                                                    >  1:551679/1‑301 (MQ=255)                                                                aaacccaTCCCACCTTACTATAAAATTGAGTGGTATACGATTGACCAAATAAATCATAACGATATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCTCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCGTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAAc                                                                                                                                                                                                                                <  2:531999/295‑1 (MQ=255)                                                                aaaaatATCTCACCTTACTATAAAATTGATTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAAc                                                                                                                                                                                                                                <  2:418304/296‑1 (MQ=255)                                                                  tCCTATCTCACCTTACTATAAAATTGAGTTGTAGACCATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACag                                                                                                                                                                                                                              <  2:519931/301‑1 (MQ=255)                                                                     tATCTCACCTTACTATAAAATTGATTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTAGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATGGATCTGTTTGAAAGAAAGCTAAAAAAGGATAGAATAGGCAAGAAc                                                                                                                                                                                                                                >  1:418309/1‑296 (MQ=255)                                                                     tATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGAAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGAAAGCATAGGCAAGAACagaaa                                                                                                                                                                                                                           >  2:1024888/1‑299 (MQ=255)                                                                          aaCCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACTAAATACTTTTATAATAGTATCTCGTTCCGTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTGTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGa                                                                                                                                                                                                                      <  2:551668/300‑1 (MQ=255)                                                                                 cgataAAATTGAGTGGTAGAAGATGGAACAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGTACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAAc                                                                                                                                                                                                                                <  1:446052/282‑1 (MQ=255)                                                                                 cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGTACCAAATTTAATTAAAGCTATCGCTCTTGTTGACAGCAATCTAAAAAAGGATAGCATAGGCAAGAAcctgtctcttatacaaca                                                                                                                                                                                                               >  2:446046/1‑284 (MQ=255)                                                                                 cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTAACGTTTTGTATATTTATTACACAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGCACCACATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAAc                                                                                                                                                                                                                 >  1:511933/1‑299 (MQ=255)                                                                                 atataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCGTCACGTTTTGTATATTTAGGACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAAc                                                                                                                                                                                                                 <  2:511926/298‑1 (MQ=255)                                                                                      aaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAACTTTAATTAAAGCTATCGCTCTTGTTGAACGCAATCTACAAAAGGATCGCATAGGCAAGAAcctgtttcttttacccatacgaa                                                                                                                                                                                                          >  2:158345/1‑279 (MQ=255)                                                                                      aaaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCAGTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAAc                                                                                                                                                                                                                                <  1:158346/279‑1 (MQ=255)                                                                                           taggggTAGAAGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGt                                                                                                                                                                                                     <  2:528302/297‑1 (MQ=255)                                                                                             gTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAACAGGATTGCATAGGCAAGAACATAGATAAGACTAACAATATAAAGAGtta                                                                                                                                                                                                   >  1:312415/1‑300 (MQ=255)                                                                                                               aataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCa                                                                                                                                                                                                                                     >  1:676838/1‑249 (MQ=255)                                                                                                               aataaatCATAACGAGATACTGTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCa                                                                                                                                                                                                                                     <  2:676826/249‑1 (MQ=255)                                                                                                                    atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGATGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGctc                                                                                                                                                                                                                                                                         >  2:224789/1‑208 (MQ=255)                                                                                                                    atCATAACGAGATACGTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGctc                                                                                                                                                                                                                                                                         <  1:224791/208‑1 (MQ=255)                                                                                                                            gagaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCttgttg                                                                                                                                                                                                                                                                   <  2:410134/206‑1 (MQ=255)                                                                                                                                  cTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATAtt                                                                                                                                                              >  2:822726/1‑301 (MQ=255)                                                                                                                                   ttttATAAGAGTATATAGTTCCTTTCATGTAAACCGCACACATGAAAAAATATGCGCTCACAAAAAGTTAAATGCTTAACCAGCTGAACGTTTGGTATATTTATTAAAAAAATGTCTTGCGCCAGCAACTCTTGATCGAATGAAGCAGGAACAATGTTCAGAATCGAACCAAAGTTAAGTAAAGCTATCGCTCTTGTGGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTc                                                                                                                                                             <  2:996963/301‑1 (MQ=255)                                                                                                                                    tttAAAATAGGATATCGTGCATTTCATTAAAACACAAAAAAGGAAAAAATATTCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATAGTTAGTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAAGATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTcc                                                                                                                                                            <  2:1046655/301‑1 (MQ=255)                                                                                                                                          ataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTACAGCTATCGCTCTTGTTGACAGCAATCTAAAAACGGATAGCATAGGCAAGAACAGAGATAAGAATCACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGtt                                                                                                                                                      >  1:567434/1‑301 (MQ=255)                                                                                                                                          ataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAAc                                                                                                                                                                                                                                <  1:905059/227‑1 (MQ=255)                                                                                                                                          ataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTATTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGAAAAAAc                                                                                                                                                                                                                                >  2:905032/1‑227 (MQ=255)                                                                                                                                               aTCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAGTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGAAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCAt                                                                                                                                                                                                                                          <  2:120465/212‑1 (MQ=255)                                                                                                                                                             attcaATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAAGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGAAATCTAAGAAAGGATAGCATAGGCAAGCACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACAATATGCATATTCCCCTGTTCACAAAACGTGGaataaga                                                                                                                                   >  1:736542/1‑299 (MQ=255)                                                                                                                                                              tttcaaccTCACACATGAAAAAATATGCTCTCACCAAAAGGTAAATTCTTAACCATCTTCACGTTTTGTATATCTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCGCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCATGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAATTTATATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTTGAAtaataa                                                                                                                                  <  2:264847/295‑1 (MQ=255)                                                                                                                                                                           caTGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGACCAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTCAAAAAACGTGGAATAATAAAAGATGAACgaga                                                                                                                     >  2:686433/1‑301 (MQ=255)                                                                                                                                                                           caTGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGtt                                                                                                                                                                                       <  2:941678/235‑1 (MQ=255)                                                                                                                                                                                   aaTATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGACTCGTACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAg                                                                                                                                                                                                                                                  >  1:820562/1‑168 (MQ=255)                                                                                                                                                                                   aaTATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGTACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAg                                                                                                                                                                                                                                                  <  2:820536/168‑1 (MQ=255)                                                                                                                                                                                               aaaaaaGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTc                                                                                                 <  1:822752/299‑1 (MQ=255)                                                                                                                                                                                                          aTTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATCGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAACCCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCACAACATTaaa                                                                                      >  1:1060909/1‑301 (MQ=255)                                                                                                                                                                                                          aTTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTaaa                                                                                      >  2:354362/1‑301 (MQ=255)                                                                                                                                                                                                                    aTCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGtt                                                                                                               >  1:1068024/1‑266 (MQ=255)                                                                                                                                                                                                                    aTCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGtt                                                                                                               <  2:1067992/266‑1 (MQ=255)                                                                                                                                                                                                                             ttttGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTGGCTCTAATGAAGCAGGAACAATGTTAAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATACAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTATAATAGTAACCGAGCTTTtaa                                                                   >  1:68972/1‑300 (MQ=255)                                                                                                                                                                                                                                 gTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAAAATTAAAATAGGAACAGAGATTTTATATaa                                                               >  2:208325/1‑301 (MQ=255)                                                                                                                                                                                                                                                   tGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTgg                                             <  1:354367/301‑1 (MQ=255)                                                                                                                                                                                                                                                     ttgttcgacagaaagattttagctAAAGAAACAAGAAAAATTATCAAAATCGAACCAAATGTAAGTAAAGCTATAGCTCTGGATGAAAGAAATTTAAAAAAGGAGAGCATAGGCAAGAACAGAGATAAGAATAACAATAGAAATAGTTTAGAGTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAg                                           <  2:736516/279‑1 (MQ=255)                                                                                                                                                                                                                                                      tgttagCCAGCAACTCTTGTTCTAACTAAGCAGGAACAATGGTCAGAAACGAAAAAAATTTAATTAAAGATATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGATATAAGAATAACAATATAAAGAGTTTAGATTATTGGGTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGt                                          <  2:953566/296‑1 (MQ=255)                                                                                                                                                                                                                                                      ctgtCGACAGCAACTCTTGACATAATGAAGCAGGAACAATGTTCAGAATCGAAACAAATTTAATTAAAGCTATCGCTTTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGt                                          <  1:817062/298‑1 (MQ=255)                                                                                                                                                                                                                                                                        gCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACAATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTggg                        >  1:360513/1‑301 (MQ=255)                                                                                                                                                                                                                                                                          tctAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGa                      <  1:686445/301‑1 (MQ=255)                                                                                                                                                                                                                                                                           atAAAGAAGCAGGAACAAAGTTCATAATCGCACCAAATTTAATTAAAGCTATCGGTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACTAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGAc                     <  1:1092024/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTCTCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGCGCAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATACACCAGATGCATATTCCCCTGTAAAAAAAACGTGGAATAATAAAAGAGGAAAGAGATTTGTGAGATAACCCTTGTCTGAACATTAAAATAGGAACGGAGAATTTATATAACCATTTTACACGTGTTGGATTAACTTGGAAATGACTTGGGACTTATaaa              >  1:384656/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     aGGAACAAATGTCAGAATCTAACCAAAGTTAATTAAAGCTAGCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGTCAAGAAAAGATATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATg             <  2:312410/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aCAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAACGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAACCGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTCGTCTGAACATTAGAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTACCGTGGCAATGCCTGGGGCTTTATCATGCGGTtt       >  1:793891/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                              gTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTGGCa  >  1:329516/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                  ATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTTCAGAATCGAACCAAATTTAATTAAAGCTATCGCTCTTGTTGAAAGCAATCTAAAAAAGGATAGCATAGGCAAGAACAGAGATAAGAATAACAATATAAAGAGTTTAGATTATTGGTTAATGCAGATAAACCAGATGCATATTCCCCTGTTAAAAAAACGTGGAATAATAAAAGATGAACGAGATTTGTTAGATAACCCTTGTCTGAACATTAAAATAGGAACCGAGATTTTATATAACCATTTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCA  >  NC_000913/2993758‑2994343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.