Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,809 Δ1 bp 79.3% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,815,8090G→.79.3% 52.7 0.0 ‑0.895intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  new base (42/33):  ref base (7/5):  total (54/41)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.76e-01

CCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGC  >  NC_000913/3815510‑3816103
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        
cgggcggggCCCCAGCGGTGGGGGTGGCAATCGGGATCCCTTTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAAGCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg                                                                                                                                                                                                                                                                                                          <  2:216751/292‑1 (MQ=255)
           aaagcgggttgggggggatacGGGAACCCTTTGTAAGCAGGGCCAAACAGAAGATCGAAGTCAATGCCGGAATCCACCAAGGCTTAAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGt                                                                                                                                                                                                                                                                                             <  2:2026/281‑1 (MQ=255)
                               cGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                                                                                                                                                                           >  1:103739/1‑300 (MQ=255)
                                     cccTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAAGGGCTTTTGCCCCCGGATTTCAGCGTAAACGCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATCAACTGGCGCTGATCTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAA‑TCGCCTCATCCATCACCTTAAAAATCATTTTACCaaca                                                                                                                                                                                                                                                                    >  2:540575/1‑297 (MQ=255)
                                        tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGCTTTCAGCGTAACCTCGCCAAACTTTGCCCCCGGCTGGCTAAGCGCAAATTCAATAAACGGGCGCTGATATGTCTTCATGCCTTCGCTCCTCCTCGTACTTTTCTACAGACCACAAACA‑GCTACCCCTCAGTCCCCTTACAACACAGttctcccaccccc                                                                                                                                                                                                                                                                 >  2:714446/1‑289 (MQ=255)
                                        tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGAGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGACAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTATTCTTACTTTTCTTCAGACAAACAAAA‑CCGCCTCATCAGCCGCCTTAAAAATCAGTTTCCCaccaccc                                                                                                                                                                                                                                                                 >  2:612369/1‑294 (MQ=255)
                                                  gggCCAAACAGCAGATAGAACTAAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCATAACAGGGCCAGATGGCGCCCGGTCTTAAACAGACCGGCGTTGAAGAAATAGAGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGACAAACTTAAACACATGCTTGCTAAGCGCGAATTCAATAAACTGGCGCTGATATGGTTTCATGACTTCGCGCCTCCTCTTATTATTC‑GCAAGGAAAAAAAAAACGAATCATCAGTCTCCATCACAATGAGttcagcagctcgtattttaagcga                                                                                                                                                                                                                                                      >  2:99710/1‑279 (MQ=255)
                                                     ccAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAACAAAA‑GCGACTCCTCAGTCCCCTTAAAAATCCTTTTGCCAACCCCCACTTCTCCGTCGc                                                                                                                                                                                                                                                    >  2:717635/1‑300 (MQ=255)
                                                      cAAACAGCAGAGCGAACTACATGCCGGAATCCACCAACGCGTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTa                                                                                                                                                                                                                                                  <  1:20316/301‑1 (MQ=255)
                                                       aaaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCCGTCGCCTTAAAAATCCGTTTGCCAGCGCCCCCTTCTTCGTAGCTa                                                                                                                                                                                                                                                  >  2:521103/1‑300 (MQ=255)
                                                                    aacaccaTGCCGGAACTGACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACGCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat                                                                                                                                                                                                                                     <  1:61091/294‑1 (MQ=255)
                                                                      cTCAATGCCGGAATCCACCAACGCTTCAGCGT‑AAAACGGCCTAACCGTGCCAGGACGCGCCCGGTATTTAACCGCCCCGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTTCACGTAAAATCGCCAAACTTTAACACCTTCTTGCTAAGCGCAAACTCCATAAACTGGCGCTGAGAAGGGTTCATGACTTCGCACCTCATCTTACCTTTCTCCCGCAAAAAAAAAGGCGGCTCAACAGTCGCCTTAAAACACCATTTGCCACCGCCCCCCTCTGctgccccaccactgattcgatac                                                                                                                                                                                                                                   >  2:569886/1‑278 (MQ=255)
                                                                            gCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTATGCGCAAATTCAATAAACTGGCGCTGATATGTTTTCCTGCCTTCGCTCCTCATCTTACTTTTCCACAGAC‑AAAAAAACGCGACTCATCAGTCCCCTTAAAAATCACTTTCCCAGCCCCCCCTTCCGCCTCCTCCCAATGTTTTCGCTTCCCCCCgg                                                                                                                                                                                                                            >  2:745133/1‑301 (MQ=255)
                                                                            gCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCACTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCCCTACAATGGATTCGATTCCCCCCgg                                                                                                                                                                                                                            >  2:111420/1‑301 (MQ=255)
                                                                             cccgaaaccaccacgccgtCAGCGTAAAACCGGCCTAACATGGCCCTGTCGCGCCCGGCAGTAAACATCCCGGCGTTGAAGAAATATGGGCTTTTGCTCCCGGATTTCACCGTAAAATGGCCAAACTTCAACACCTGCTGGCTAAGCGCAAATGCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTCCTTTTCTACAGACAAACAAA‑‑TCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                                                                                                                                                                                                                           <  2:707287/282‑1 (MQ=255)
                                                                                gAATCCACCAACGCGTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGcc                                                                                                                                                                                                                         <  2:480271/300‑1 (MQ=255)
                                                                                     caccaACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAGAATCAGTTTGCCAGCGCCGCCTTATGCGTCGCTCAAATGGATTCGATTACCCTCGGCCCAGAG‑c                                                                                                                                                                                                                   >  2:77344/1‑300 (MQ=255)
                                                                                            gCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCACGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAGCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTGCATGCCTTCGCCCCTCATCTTACTTTTCCACCGACAAAAAAAA‑GCGACCAATCAGTCGCCTTAAAAATCATTTTGCCACACCCGCCCACTGCGTGCCTACAATGGATCAGCTTCCCCTCGGGcacaccccacccaaa                                                                                                                                                                                                            >  2:277079/1‑287 (MQ=255)
                                                                                             ctgcAGCGGAAAAACGGCCGAACAGGGCCAAGTCGCGCCCGGTATGAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACGTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTGCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTGCTGCGTCGCTACCATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGa                                                                                                                                                                                                           <  1:730614/297‑1 (MQ=255)
                                                                                                 cgCGTAAACACGGACGAACACTGCCAGATCGCGCGCGGTATTAAACAGCCCGGCTTTGAAGAAGTAGTGGCTGTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACCCCTGCTTGCTAAGCTCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTCCTTTTCTACCGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAg                                                                                                                                                                                                       <  1:117445/300‑1 (MQ=255)
                                                                                                   cGTAAAACCGGCCTAACCGCGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAg                                                                                                                                                                                                    <  2:723869/301‑1 (MQ=255)
                                                                                                            ggCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCGCCGACTTCTCCGTCGCTACAATGGCTTCGATTCCCCTCTGGCCCGAG‑CCACAAAGATGAGTAGCTCTTCATg                                                                                                                                                                                           >  2:92894/1‑301 (MQ=255)
                                                                                                                 aaCAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCCAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGCTACAATGGATTCCATTCCCCTTGGGCCATAG‑CCAACAAGATGAGTAGCCCTACATGGGTg                                                                                                                                                                                       >  1:137949/1‑300 (MQ=255)
                                                                                                                   cAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTTCCAGCGCCCCCTTCTCCGTCGCTCCACTGGCTTCGATTCCCCCCGGGCCAGAC‑CCACCACGATGCGTCGCCCTTCATggctcaa                                                                                                                                                                                     >  2:429666/1‑295 (MQ=255)
                                                                                                                     gTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAC‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                                                                  >  2:140262/1‑301 (MQ=255)
                                                                                                                     gTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCG‑CCACCAAGCTGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                                                                  >  1:177050/1‑301 (MQ=255)
                                                                                                                     gTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCCCATCTTACTTTTCGTCAGACAACAAACA‑GCAACCCATCATTCGCCTTAACCATCAGTTTGCCAGCGACGCATACCGCATCACTACTATTGCACCGATTCCCCTCGTGCCAGAA‑CCACCAAGACGCCTTGCTATTCACGGGTACACgg                                                                                                                                                                                  >  2:233742/1‑301 (MQ=255)
                                                                                                                     gTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGACAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATAGGGTTTCATGCATTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTACAAATCAGTTTGCCAGCGACGCCTTCTGCGTCGCTACAATGGATTCGATTCCACTAGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                                                                  >  1:85666/1‑301 (MQ=255)
                                                                                                                      tGCCAGATCGCGCCCGGTATTCAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCCGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTACGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCCCCTAATCTTACTTTTTTACCGACAAAAAAAA‑GCGACTCATCAGTCGCCTTCACACTCAGTTTGCAAGCGCCGCCTTCCCCGTCGCCACAATGCTTTCGCTTACCCACCGGCCGGAGCCcaacaacttgacacctccgcctgggggaacgg                                                                                                                                                                                   >  2:212541/1‑274 (MQ=255)
                                                                                                                                 ccccGGTATTAAACAGCCCGGCTTTCAAGTAATAGGGCCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATCAACTGGCGATGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                                                                                                                                                                      <  1:540598/300‑1 (MQ=255)
                                                                                                                                  cccGGGAGTAAACTGCCCGGCGTTGCAGTAAGTGGGTGTGTTGCGCCCGGATATCAGCGTAAACGTGTCAAACTTGAACACCTGCTTGCTAATCTCAAATTCAATAACCTGGCGCTGATATGTTTTCAGGCCTTCGCTCCTCATCTTACTTTGCTACAGACAAAAAAAA‑GCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCGTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                                                                                                                                                                      <  2:233781/300‑1 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGGTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTACGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGA‑AAAAAAAAAGCGACTCATCAGTCGCATTAAAAATTAGTTTGCAAGCGGCGCCTTCTACGTCGCTAAAATGGATTGAATTCCCCTCGAGACAGAG‑CCAACAAAGTGAATAGCTCTAAATGGTTGAACAGCTCGCACTCTGCCGta                                                                                                                                                                  >  1:449387/1‑299 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCACATTCAATCAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGACAGGG‑CCAACAAGATGAGTAGTCCCTACTGGGTGACCGGCGCGCCTTCTGCCgcc                                                                                                                                                                  >  2:751832/1‑298 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAACAAAAA‑GCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCTCCGCCTTCTGCGTCCCTACAATGGATTCGATTCCCCTCCGGCCAGCG‑CCAACAAGATGAGTAGCCCTTCTTGGTTGCACgcgctcacttttcccctc                                                                                                                                                                  >  2:16138/1‑283 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGTCCAGAG‑CCAACAAGATGAGCAGCTCTTCATGGTTGCACGGCCCGCCTTCTGCCgc                                                                                                                                                                   >  2:40130/1‑299 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGCTGAGTAGCTCTTCATGGGTGCACGGCTCGCCTTCTGCCGt                                                                                                                                                                   >  1:636886/1‑300 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGACTcccc                                                                                                                                                                                                                                >  1:166412/1‑240 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAAGAACATGCGTAGCTCTTCATGGGTAAACGGCTCGCCTTCTGCCgcc                                                                                                                                                                  >  2:243918/1‑298 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTgg                                                                                                                                                                                                                                            >  2:736044/1‑227 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATgg                                                                                                                                                                                                                                            <  1:736071/227‑1 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGCTTCCCCTCGGGCCAGCG‑CCCACCAGCAGAGTAGCTCTTCCTGGGTAAACGGCTCCCCTTCCGCCgcc                                                                                                                                                                  >  2:523855/1‑298 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGACGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCCGAG‑CCAAACAAATGAGTAGCACTTCACGGGTAACGGGCCCGCCTTCTGACGTc                                                                                                                                                                  >  2:73432/1‑300 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTCAACACCTGTTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATTTTGTTTCATGCTCTCGCTCCTCATCTTACTTTTCTACAGATAAAAAAAA‑GCGATTCATCAGTCGCCCTAAAAATCAGTTTGCCAGCGTCGCCTTCTGCGTCGCTACAATGGATTCGATTTCCCTCGGGCCAGAC‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGta                                                                                                                                                                  >  1:130594/1‑299 (MQ=255)
                                                                                                                                      gTATTAAACAGCCCGGCGTTGAAGAAATAGGGAATTTTGCGACCGGATTTCAGCGTAAACTCGCCAAACTGTAACACATGCTTGCTGAGCGAAAATTCAATAAACTGGAACTGATATGGTTTCATGCCTTCGCTACTAATCTTACTTTTCTACAGACAAAAAAAA‑GCGACACATCAGTCGCCTTAAAAATAAGTTTGCGAGCCACGCCTTCTGCGTCGCTACAATGGGTTAGACTCCCCTAGGGCCAGAG‑CAAACACAATGCGCAGCTATTCATGGGTGAACGGCGAGCCTTCTGCCGTc                                                                                                                                                                  >  1:231632/1‑300 (MQ=255)
                                                                                                                                       taagaaaCCGCCCGGCGTTGAAGAAATGGGGGCTGTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAAGCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATTGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc                                                                                                                                                                 <  1:92898/296‑1 (MQ=255)
                                                                                                                                        tttacacgacccAGCTTTAAAGAAATGGGGGCTTGTGCGTCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAAATTAAATAAGCTGACGCTAATAAAGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTTCGAACAAAAAAGA‑GCGACTCGTCAGTAGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGGACGGCTCGCCTTCTGCCGTcccc                                                                                                                                                               <  2:130589/292‑1 (MQ=255)
                                                                                                                                        aTTAAACAGCCCGGCTGTGAAGAAATAGGGGCTTTTTCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                                                                                                               <  1:111425/301‑1 (MQ=255)
                                                                                                                                           aaaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑CCGACTCATCACTCACCTTAAAAATCAGTTTTCCAGCTCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCCGCCCCACC‑CCCACAAGCTCAGTAGCTCTTCATGGTTGACCGACCCGCCTCCCTCCGCCCCCtc                                                                                                                                                             >  2:709560/1‑299 (MQ=255)
                                                                                                                                                ccccGGCGGTGAAAAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACCCGCCAAACGTTAACACCTGCTTGCTAACCGCAAATTCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                                                                                                                       <  2:183294/300‑1 (MQ=255)
                                                                                                                                                 cccGGCTTTGAAGAAATAGGTGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTAATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                                                                                                                       <  1:569909/301‑1 (MQ=255)
                                                                                                                                                     ccggtGAAGAAAGCGGCGCTTTTGGCCCCCGACTTCAACGGAAACTCGCCAAACTTTTACACCTTCTTGCCAAGCGCAAATTCAATCAACGGCGGCTGATATGGGTTCATGCCTTCGCTCCTACTCTTACTTTGCTACAGACAAAAAAAA‑GCGACTCAGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAGTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga                                                                                                                                                  <  2:59172/297‑1 (MQ=255)
                                                                                                                                                       gTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGGCGCCTTAAAAATCAGTTTGCCAGCACCGCCTCCCTCGTCGCTACAATGGATTCAATTACCCTCGGCCCAGAC‑CCAACCAAACGAGTACCTCTTCTTGGGTGAACTGCCCGCCTTCTCCCGCCCCCCCCACTCCCatgc                                                                                                                                                  >  2:703922/1‑299 (MQ=255)
                                                                                                                                                        gtGAAGAAAATGGTGCTTCTGGCCCCGGATGACAGTCTAATCTCGCCAAACTTGAACACTTGCTTGCTAAGCGCAAATTCAATCAACGTGCGCTTATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCGACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAGATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGAGTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                                                                                                                <  1:212548/299‑1 (MQ=255)
                                                                                                                                                          gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTACAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACCATGGATTCGATCCCCCTCGGGACCGAG‑ACAACAAAATGAGTAGCTCTTCATGGGTGCACGCCTAGCCGTCTGCCGTCCCATGCACCTCAatgcttag                                                                                                                                              >  1:233301/1‑295 (MQ=255)
                                                                                                                                                             gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGAGCCGCCTTCTGCGTAGATACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTATCCCGCCCCCTGCACTTCAatgatgagacc                                                                                                                                           >  1:630533/1‑295 (MQ=255)
                                                                                                                                                             gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGCTTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGACAAAACAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTGGATTCGATTCCCCTCGGGCCAGGT‑CCAAAACGATGAGTAGATCTTCATGGGTGCACGGCTACCCCTCTGCCGTCCCCGGCAACACAAGGAGGCGcca                                                                                                                                           >  1:119179/1‑299 (MQ=255)
                                                                                                                                                                             cgcCCGGATTTCAGCGTAAACCCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                                                                                                                           <  1:521126/300‑1 (MQ=255)
                                                                                                                                                                                ccctaTTTCATCGTAACCTCCCGAATCTTTAACACCTCCCTGCTAAGCCCAAATTCAATAAACTGGCGCTTATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCTCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGCTTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGATGCGCCCGTCTTCGGTCATCACTACGt                                                                                                                       <  2:85662/297‑1 (MQ=255)
                                                                                                                                                                                     ttttaccttcaaCTCACCCAACTTTAACACTTGCTTTCTAAGGGCAACTTCACTAATCTGGCCCTGATATGGTGTCAGGCCTTATCTCGTCATCTTACTTTCCTACAGACACAAAAAA‑GCGACGCATCATCGGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAGGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCAGGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGGTCATg                                                                                                                  <  2:122621/291‑1 (MQ=255)
                                                                                                                                                                                          gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCTGGCCAGAG‑CCAACAAGCAGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGTCCTCACTACGTTCATGTCGGt                                                                                                             >  1:531798/1‑301 (MQ=255)
                                                                                                                                                                                                   cGCCAAACCTGAACCCCTGCTTGCTAAGCGCAAATTCAATATAATGGCGCTGATACGGTTTCAGTCCTTCCATCCTCATCTTACTGTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCATAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGc                                                                                                   <  1:185196/301‑1 (MQ=255)
                                                                                                                                                                                                          cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAg                                                                                                                                                                                                                                                                                              >  1:99201/1‑110 (MQ=255)
                                                                                                                                                                                                          cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTACGTTACTGTCGGT‑CTCTGCGGCCGAGt                                                                                              >  1:584998/1‑299 (MQ=255)
                                                                                                                                                                                                          cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCACAG‑CCAACAAGCTGAGTAGCTCTTCCTGGGTGAACGGCCCGCCTTCTGCCGCCCCCTGc                                                                                                                                                            >  2:365119/1‑238 (MQ=255)
                                                                                                                                                                                                          cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGACCgg                                                                                                                                                                                  >  2:502278/1‑216 (MQ=255)
                                                                                                                                                                                                          cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                                                                  <  1:502301/216‑1 (MQ=255)
                                                                                                                                                                                                          cTTGAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAg                                                                                                                                                                                                                                                                                              <  2:99197/110‑1 (MQ=255)
                                                                                                                                                                                                            ttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAATA‑GCGACTCATCAGTCGCCTTAAAAATCCGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTTGATTCGATTACCCGCGGGCCAGTG‑CCAACACGATCAGTAGCTCTTCATGGATGAACGGATCGCCGTCTGCCGTCCAATCGACTTCAATGATGCGCCCGTACTCGGTCATCACTCGGGACATCTCAGT‑CTCTGCGGCAGCGtagtt                                                                                          >  1:647629/1‑297 (MQ=255)
                                                                                                                                                                                                               acacCTGCTTTCTAAGCGCAAATTCAATACACTTGCGCTGATATGGTTTCATGCCTTCGCTCCTAATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGCTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAAc                                                                                       <  1:73436/301‑1 (MQ=255)
                                                                                                                                                                                                                caccgtgttgctAAGCTCAAATTCTATAAACTGGCTCTGAGATGGTATCATACCTTCGCTCCTCATCTTACTTTACCACACACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGCTACGATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAATAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACg                                                                                      <  2:137943/294‑1 (MQ=255)
                                                                                                                                                                                                                      cttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                <  2:177043/301‑1 (MQ=255)
                                                                                                                                                                                                                      cctgtcaatctcAAATTCAAGAAACTGTCGCGGATAGGGGTCCATGCCTTCGCTGCCCAACTTACGTTTCTCCAGACAAAAAAAA‑GCGACGCTTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGTCTGCGTGGCTACAATGGATTCGATGCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCGTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                <  2:61070/290‑1 (MQ=255)
                                                                                                                                                                                                                        tgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTGTCATGGCTTCGCTCCTCATCTTACTTTTCTACACACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAg                                                                              <  1:429677/301‑1 (MQ=255)
                                                                                                                                                                                                                         tctaacccaaaaTCCAAAAAACTGGCGCTGTATTGGTTTCATTCCTTCGTTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAg                                                                              <  2:103735/292‑1 (MQ=255)
                                                                                                                                                                                                                          ctAAGCGCAAATTTAATAAACTGGCGCTGATATGGTTGCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGAt                                                                            <  1:717661/301‑1 (MQ=255)
                                                                                                                                                                                                                             cgcccAAATTCAATCACCTTGAGCTGATATTGTTTCATTCCTTCCTTCCTCATCTTCCTTTTCTACCGACAACAAAAA‑TCTACTCATCAGTCTCCTTAACAATCAGTTTGCCATCGCCGCCTTCTGCTTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGTTGAGTAGCTCTTCATGGGTGAACGGCTCGCTTTCTGCAGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGc                                                                         <  1:99714/297‑1 (MQ=255)
                                                                                                                                                                                                                                       cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAACTCTTCATGGGTGAACGGCCCGCCTTCTGCCGTCCCCTGCACTTCAATGACACGCCCGCCCTCGGTCCTCCCCACGTAACTCTCGGA‑CCCTGCGCCAGAGTCTTCAGCGTATTCCAAACAGCAAACCCCtg                                                                >  2:652712/1‑299 (MQ=255)
                                                                                                                                                                                                                                           aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                                                                                                                  <  1:52741/231‑1 (MQ=255)
                                                                                                                                                                                                                                                ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCACCAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCCATACGTTCCTGTCGGTCCTCT‑CGGCAGAGTCTTCCACGTACTCCAGATAGCCACCCGCTTCCCCgctgg                                                       >  2:679140/1‑296 (MQ=255)
                                                                                                                                                                                                                                                ggCGCTGATATGGTTGCAGGCCGTCTCTCCGCTACTTACTGTCCTACAAACAATAAAAA‑GCGACTCATCTGGCGCCGTAAAACGCAGTTGGCCAGCGCCGCCTTTTGCGTCGCTACAATGGATGCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGGAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCg                                                           <  1:679166/296‑1 (MQ=255)
                                                                                                                                                                                                                                                      gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCCGATCCCCACCCCCTTCGCCGTTcccactcc                                                 >  1:550899/1‑296 (MQ=255)
                                                                                                                                                                                                                                                            gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGCCTCATCAGTCGCCTTAAACATCATTTTGCCAGCGCCCCCTTCTGCGTCTCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCATCCCCTGCACTTCAAGGATGCGCCCGCCTTCGGTCATCACTACGTTCCTGTCCGC‑CTCTGCGGCAGTGTCTTCACCGTTTTCCAGCTCGCAAACCGCTTCGCCGTTCACACTCCCgcccaa                                          >  1:466692/1‑296 (MQ=255)
                                                                                                                                                                                                                                                            gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGCGTCTTCAACGTATTCCAGATCGCCAACCGCTTCGCCGTTCCCAATTCCGACAGa                                          >  1:522372/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                    ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGGCTTCGGTCCTCCCTCCGTTCACGTCGGT‑CTCTGCGGCCGATTCTTCAACGTATTCCATATCCCCAACCGCTTCGCCGTTCACCATTCCCACAGACAccccgg                                  >  2:79404/1‑296 (MQ=255)
                                                                                                                                                                                                                                                                         cctccgcATCGTACTTTTCTACATACAAAATAAA‑GCGACTCATCAGTCGCCTTAAAAAGCAGTTTGCCAGCGCTGCCTTCTTCGTCGCTACAATGGATTCGCTTCCCCGCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGTCTCGCCTTCTGCCCTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCTGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTGCACAATTCCGACAGAAACTGCGGCTAcc                             <  1:751859/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ctcctcATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCCTGGGTGACCGGCTCACCTTCTGCCTTCCCCTGCACTTCAATGATTCGCCCGTCTTCGGTCTTCACTACGTTCATGTCGGT‑CTCTGCGGCACAGCCTTCAACGTATTCCAGCTCCCAAACCGCTTCCCCGTTCCCCATTCCGCCCGAAACTGCGGCTCCCa                            >  2:150300/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                          ctcctcATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                                                                                                            <  2:224973/206‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ctcATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGT‑ATCTGCGGCAGAGTCTTCAACGTATTCCACATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAAATGCGGCTACCacc                          >  1:409693/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                aTCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGCTCGCAAACCGCTTCGCCGTTCACAATTCCGACCGAAACTGCGGCTACCATCCCtt                      >  2:106658/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                 tCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGTTTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga                                                                                                                                                  <  2:475346/177‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGTTTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga                                                                                                                                                  >  1:475360/1‑177 (MQ=255)
                                                                                                                                                                                                                                                                                     tacattctgacagaacaaaaaaAGCAACTCATCAGTCACCTAAAAAACCGGTTTGCCAGCGCCGCCTTCTGCTTCGCTACAATGGATTCGATGCCCCTCGGGCCAGAG‑CCAACAAGATGCGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCAt                  <  2:630508/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   aaaaaaaaGG‑GACCCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAg   <  2:584974/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     aaacaaCGCGACTCATCCGGCGCCTTCAAAATCCGTTTGCCAGCGCCGCCTGCCGCGTCGCTACTATGGATTCGATTCCCCTCGGGCCAGGG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGGCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGc  <  1:277085/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     aaaaaaAGTGACTCATCAGTCGCCTTAAAAACCAGTGTGCCAGCGCCGCCTGCTGCCTCGCTACAATGGCTTCGAGTCCCCTCGGGCCAGAG‑CCAACAAGATGAGGAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGGCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTAGTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGc  <  1:652738/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                      aaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTATGTTCCTCTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCACACCGCTTCGCCGTTCACCCTTCCGCCAGAACCTGCGGCTCCCCTCCCTTTCATCGGTTCGGTTTTCat   >  2:490725/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        
CCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAG‑CCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGT‑CTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGC  >  NC_000913/3815510‑3816103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.