Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,621,022 C→A R39L (CGC→CTC)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,621,0220CA98.2% 174.6 / NA 57R39L (CGC→CTC) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (29/27);  minor base T (1/0);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACT  >  NC_000913/1620736‑1621318
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                        
aCTATATTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGc                                                                                                                                                                                                                                                                                            <  1:54149/301‑1 (MQ=255)
               cacCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCACCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAAATGATGGTTAATCCAAACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTTCGTCAAAAACGATAGCCGGAAAAGCGAACAGGAAAAACAAAAACCCGGCCAACAtatggctgggacac                                                                                                                                                                                                                                                                             >  2:96816/1‑288 (MQ=255)
                  cAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGACATGTTa                                                                                                                                                                                                                                                                          <  1:381362/301‑1 (MQ=255)
                        cccAAATATCACTAAGGCCGTCCCCGCCAATTGTGTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGGGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGc                                                                                                                                                                                                                                                                    <  1:478535/300‑1 (MQ=255)
                                                            gCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACACCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGAAAAAGCGACCAGAATAAAGAACAAACCGGCCAACATCAACAGTGGCAGGTTATAAAGTCCCCCTGTGATGACCACAAAATTCAGCCCCCAttcc                                                                                                                                                                                                                                >  2:442456/1‑298 (MQ=255)
                                                                              gAAAGTAAACGCGCCAATCAAGAAAGTAAAAAACGCCTGTGCCTGTAAAACCAGCAAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAAGTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACca                                                                                                                                                                                                                                                    <  2:532412/263‑1 (MQ=255)
                                                                                    aaaCGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGGTACTGGCAAAAGAAAGAACAGCACACTGCGCACAACTGATGGTTAATCCAAACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGAGACCAGCATAAAGAGCAAACCGGACAGCATACGCGGTGGCATGGTATGACACCCCACTATCATGAAAACAAAAGTTATCCACCATCCAACCAACACCAGTaacaccaacacc                                                                                                                                                                                                        >  2:296279/1‑301 (MQ=255)
                                                                                                        tAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGCGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATATAGCCCCCATACAACCCCTACCAGTCGCGACAACACCCCATCTTTTcgcccacttcc                                                                                                                                                                                    >  2:335228/1‑293 (MQ=255)
                                                                                                         aaaaaaCGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAACAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCCGCATCAGCGGTGGCATGTTCTGAAGCCCCACTATGATGACCACAAAATTTAGCCACATTACGACCACTACCAGTCGCGGCAAACCCACATCTTCTcgccaaatcccc                                                                                                                                                                                   >  2:547456/1‑292 (MQ=255)
                                                                                                                gcccggacctgtAAAACCAGCCAAGCCAGTCCAGCAGGAAAACCGAACTTAATGGCACCAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctc                                                                                                                                                                            <  1:96835/294‑1 (MQ=255)
                                                                                                                          gtAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACGGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt                                                                                                                                                                  <  1:255076/301‑1 (MQ=255)
                                                                                                                            aaCACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGACACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGACAGCATCAGCGGTGGCATGTTATGACGCCCCACTTTGATGACCACAAAATTTAGCACCCATACGACCACTACCAGTAGCGCCAACACACCATCTTTTAGCGACATTCTACCGCCTCTGAATTTCATCt                                                                                                                                                                >  1:37542/1‑301 (MQ=255)
                                                                                                                                      aaGCCAGTCCAGCAAGCATCCCGAAGTTAATGGCACAAAAAAGAAAAGCAAACCGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCa                                                                                                                                                      <  1:442564/301‑1 (MQ=255)
                                                                                                                                        gCNAGTCCNGNNNNNNNNNNNNAGTTAATGNANAAAAAAAGANNANCAAACGGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcccc                                                                                                                                                                                                      <  1:45382/251‑1 (MQ=255)
                                                                                                                                          cAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCCTCTTTTGTAACCAATc                                                                                                                                                   >  1:364369/1‑300 (MQ=255)
                                                                                                                                                          ccGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCCGCGGTGGCATGTTATGAAGACCCACTTTGATGACCACAAAATTTAGCTCACATACGACCACTAACAGTAGCGCAAACACCCAACCTTTTCGCGCCATTCTACAGACTCTCAATTTCATCTTTTATAACTAATCAACtgtatgtaatttat                                                                                                                                  >  2:495555/1‑288 (MQ=255)
                                                                                                                                                           cGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATa                                                                                                                                                                                                                                  <  1:311209/204‑1 (MQ=255)
                                                                                                                                                           cGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGATAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAACAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATa                                                                                                                                                                                                                                  >  2:311125/1‑204 (MQ=255)
                                                                                                                                                           cGAAGTTAATGGCAAAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCCGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGTAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt                                                                                                                                 <  1:547586/301‑1 (MQ=255)
                                                                                                                                                            gAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGAACACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTAc                                                                                                                                                                                  >  1:187527/1‑251 (MQ=255)
                                                                                                                                                                  aaTGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCAACTTTGATGACCACAACATGTAGCCCCCATAAGACCACAACCAGTAGCGCCAACACCCCCTCGTTTCGCAACATCCAACCGCCTCTGAATTCCATCCTTTTTACACAATCCAATTAGCTGATTTAACtttttttt                                                                                                                          >  2:449878/1‑301 (MQ=255)
                                                                                                                                                                   aTGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTAAGTGGTACTTTCGGTCGTGCGACAAAGAAGATAGCCGGAAAAGCGACCAGCATAAAGTGCCAACAGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACGCAAAAATTTAGCCCCCAGACGACGACTACCGTTAGCGCCAACACCAAGTCTTTTCGCGTCATGATACCGTCTCGAAAGTTCATTTTATTTAGCAAATCAACTATGCTCAAATTACTTGTCattt                                                                                                                         >  1:292903/1‑300 (MQ=255)
                                                                                                                                                                     ggCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCACCCATACCACCACTAACAGTAGCGCCAACAACCCCTCTTTTCGCGACATTCTACAGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaat                                                                                                                       >  1:16383/1‑300 (MQ=255)
                                                                                                                                                                      gCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGCTGACCACAAAATTTAGCCCCAATACCACCACTACCAGTAGCCCCAACACCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCCTTTGTAAGCCACCAACTTAGCTGAATTTCCTTTTCTTTCAc                                                                                                                       >  2:251418/1‑300 (MQ=255)
                                                                                                                                                                       caaacaaaagaaaagCAACCTGCGAAAAACGGATGGTTATCCCAAACCCAAACAGCAAAATAAGAGGTACGTGCGGTCGTGAGACAACAAAGATAGCCGGAAAAGCAACAAGCATAAAGAGCAAACCAGACAGCATCAGCGGTGGCATGTTATGCAGCCCCCCTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAg                                                                                                                     <  2:232278/296‑1 (MQ=255)
                                                                                                                                                                               agaaaagCAAACAGCGCAAAACTGAAGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCTGGGGCATGTTATGAAGCCCCACTTTGATGACCACAACATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCg                                                                                                             <  1:296362/301‑1 (MQ=255)
                                                                                                                                                                                  aaaaCAAACTGCGCAAAACTGATAGTTAGACCATACCCCAGCAGCAAATTAAGTGGTACTTTCTGTCGCGCAACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTa                                                                                                          <  2:37538/301‑1 (MQ=255)
                                                                                                                                                                                     gCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTc                                                                                                       >  1:132180/1‑301 (MQ=255)
                                                                                                                                                                                            gcgcAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAATTTTCTTTAACCGTTGATTCTTTAGTTGCCGGtt                                                                                                >  1:403650/1‑301 (MQ=255)
                                                                                                                                                                                               cAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACACAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACACCATCCTTTCCCGACATTCTACCGCCTATGAATTGCCTCTTTTTTAAGCAATCACCTTGGTTGAATTTACTTTTCTTTTAAAGTTTAttattatgtcgtcggtgtac                                                                                             >  2:421225/1‑283 (MQ=255)
                                                                                                                                                                                                 aaaCTGATGGGTAATCCAAACCCCATCAGCAAAGTAAGTTGAACTTTCGGGCGTGGGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCAGCAGCTGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacgac                                                                                           <  1:98568/301‑1 (MQ=255)
                                                                                                                                                                                                     cgggggctcttccgatctccAGAAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCa                                                                                       <  1:416926/283‑1 (MQ=255)
                                                                                                                                                                                                         ggTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACGTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAACGCCAACACCCCATCTTTTCGCGACATTCAACCGCCTCTGAATTTCATTCTTTGTACGCCATCAACTTAGCTGAATATCCTTTTCTTTAACAGTTTATTCATTAAATGCCGGTTACGCCGGCATTa                                                                                    >  2:118695/1‑300 (MQ=255)
                                                                                                                                                                                                          gTTAATCCATACCCCAGCAGCAAATTCAGTTGTACTTTCGAGCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCCAACCGGCCAGCATCAACGGTTGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAAt                                                                                  <  2:403557/301‑1 (MQ=255)
                                                                                                                                                                                                              aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCCTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAATAGTTGATTCGTTAGTCGCCGGTTAAGACGGCATTAATtcgc                                                                              >  2:58294/1‑301 (MQ=255)
                                                                                                                                                                                                              aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTAATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGTTTACGACGGCATTAATgcgc                                                                              >  1:39379/1‑301 (MQ=255)
                                                                                                                                                                                                                ccATACCCCAGCAGCAAATTCACGGGTACGTTCGGTGGGGCGAAAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcgc                                                                              <  2:364279/299‑1 (MQ=255)
                                                                                                                                                                                                                    aCCCCAGCAGCAAATTCCGTGATACTTTAGGGCGGGCTACAAAAAAGATAGCAGGAAAAGCGACCAGCAAAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACTCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAAt                                                                          <  1:454938/299‑1 (MQ=255)
                                                                                                                                                                                                                    aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa                                                                         >  1:114500/1‑300 (MQ=255)
                                                                                                                                                                                                                       ccAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGTTTCGTTAGTCGCCGGTTACTACGGccacatcggaagtgcgtagg                                                                     >  2:416829/1‑282 (MQ=255)
                                                                                                                                                                                                                         agcagcAAATTCAGTGGTACTTGCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGc                                                                   <  1:58302/301‑1 (MQ=255)
                                                                                                                                                                                                                            agcaAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCaa                                                                                                                                                     >  1:222499/1‑216 (MQ=255)
                                                                                                                                                                                                                             ccaAATGCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtata                                                               <  2:132149/300‑1 (MQ=255)
                                                                                                                                                                                                                                                          aCAAAAAAGAAAGCCCGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTa                                  <  1:335314/301‑1 (MQ=255)
                                                                                                                                                                                                                                                           cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAATCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGTTTACGCCGGCATTAATTCGCAAATAAGTAGCTATACTTCGGCTTTTTGCTATGCTTTTTCttttt                                 >  2:339908/1‑299 (MQ=255)
                                                                                                                                                                                                                                                           aaaaaaaGAAAGCCGGACAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTAc                                 <  2:39375/300‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaaaaaACTAACCGGAAAAGCGCCCAAAAAAAAGAGCCCACCGGCCAGCCTCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACa                                <  2:114477/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aTAGCCGGAAAAGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATAATTCGGATTTTTGCCATGCTATTTCTTTACATCTCTa                          >  1:203947/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       ccGGAAAAGCAACCAGCATAAATAGCAAACCTGCCAGCATCAGCTGTTGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCCTACGACCACTACCAGTATCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAATCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTaaaaca                     <  1:495676/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCgg                                                                                                  <  2:254290/217‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGACCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCgg                                                                                                  >  1:254361/1‑217 (MQ=255)
                                                                                                                                                                                                                                                                                 aaCCAGCATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACg           <  1:251488/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       cATAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACCGTTGATTTGTTAGTCGCCGGTTACGACGGCCTTAATGCGCAATGACGTCGCTATACTTCGGATTTTTGCTATTCTATTTCTTTAAATCTATAAAACAAAACATAACGAAAccc     >  2:213480/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGcc                                                >  1:554907/1‑256 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCATACGACCACTACCATTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAATCAATCAACTTAGCTGAATTTACTTTTCTTTAACATTTGTTCCGTTAGTCTCCGGTTTACACGGCATTAATTCGCAAATAAGTCGATATACTTCGGATTTTTTATATGCTTTTTTTTTTCATCTTTCAAAAAAAAAAAAAAGaaaaacaat  >  2:289966/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGAGCAAACCGGCCAGCACCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAACTTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGcc                                                <  2:554776/256‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           aaGAGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGATTTTACTTTTCTTTAACAGTTGATTCGTTAGTCGACgg                                                                                                  >  1:506725/1‑204 (MQ=255)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                        
ACTATCTTCGATTAACACCAGTACGCCAAAAATCGCTAAGGCGATCCCCGCCAATTGTTTGCCATGCAGTCGCTCCCCGAAAGTAAACGCGCCAAGCATGATAGTAAAAAACGCCTGTGCCTGTAACACCAGCGAAGCCAGTCCAGCAGGCATACCGAAGTTAATGGCACAAAAAAGAAAAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACT  >  NC_000913/1620736‑1621318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: