Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,233,5040TA100.0% 105.2 / NA 34V470D (GTT→GAT) USA300HOU_1157non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/15);  total (19/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAA  >  CP000730/1233368‑1233634
                                                                                                                                        |                                                                                                                                  
ggTGAACGTGTTGAAGGTGGTGACAGTGTTGAGGTTGGTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACg                                                                                                                                <  1:441123/141‑1 (MQ=255)
 gTGAACGTGTTGAACGTGGTGACAGTTTTGATGTTGTTATATCAAAGGGCGCTGGAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTACAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGa                                                                                                                               <  2:335458/141‑1 (MQ=255)
   gAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt                                                                                                                             <  1:167140/141‑1 (MQ=255)
     aCGTGTTGAACGTGGTGACAGTGTTGATGTGGTTATATCAAAGGGCCCGGAAAAGGTTAAAGTGCCAAATGTCCTTGGTTTACCTAAGGATGAAGCGTTGCAGAAATTAAAGTCGTTAGGTCTTAAAGATGATACGGTTGa                                                                                                                           >  2:302539/1‑141 (MQ=255)
        tgtTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTAATTGGTTTACCTAAGGAGGAAGACTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGaaaa                                                                                                                        >  1:352335/1‑141 (MQ=255)
             aaCGAGGTGACAATGATTATGTTGTTATATCAAAAGGCGCTGAAAAGGTCAAAATGCCAAATGTCAGTGGTTTACCTAAGGAAGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGtat                                                                                                                   <  2:142327/141‑1 (MQ=255)
                     gACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGCGGAAGCCTTGCAGAAATTAAAATCGTTGGGTCTTAAAGATGATACGATTGACAAAGTATATcataat                                                                                                           >  1:49594/1‑141 (MQ=255)
                            ttGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAgcgc                                                                                                    >  2:257179/1‑141 (MQ=255)
                                            aaaGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGc                                                                                    <  1:401366/141‑1 (MQ=255)
                                             aaGGACCCTGAAAAGGTTAAAGTGCCAAATGGCATTGGTGTACCTAAGGTGGAAGCCTTGCAGAAATTAAAATCGTTAGTTCTTAAAGAAAAAAAAAAAAAAAAAGTATAGAATAATCAAGCGCCAAAAGGATACAATGCa                                                                                   <  2:280971/141‑1 (MQ=255)
                                              aGGGCCCTGAAAAGGTTAAAATGCCAACTGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAGAAGTATAGAATAGTAAAGACCCAGAAGAAAAAATTCCaa                                                                                  >  2:383380/1‑141 (MQ=255)
                                              aGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCaa                                                                                  >  1:110060/1‑141 (MQ=255)
                                                ggCGCTGAAAAGGTTAAAATGCCGAATGTTATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTTATATCGTTATGTCTTAAAGATGATACGATTGTAAGAGATTATACTAATCAAGAGACAGAAGGATACATTGCaaga                                                                                >  2:22900/1‑139 (MQ=255)
                                                  cccTGAAAATGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAACGGATACATTGCAAATCa                                                                              >  1:338742/1‑141 (MQ=255)
                                                    cTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaaa                                                                            >  2:145600/1‑141 (MQ=255)
                                                     ttaaaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGGTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAg                                                                           <  2:356752/139‑1 (MQ=255)
                                                     tGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCCAAAGAATACATTGCAAATCaaaa                                                                           >  2:72470/1‑140 (MQ=255)
                                                       aaaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgtg                                                                         <  1:18314/141‑1 (MQ=255)
                                                        aaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAACTTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgtgt                                                                        >  1:358882/1‑141 (MQ=255)
                                                              tAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAGAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGc                                                                  <  1:38417/141‑1 (MQ=255)
                                                                       aaaTGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATAAAGCGCTATTAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGa                                                         <  1:243143/141‑1 (MQ=255)
                                                                           gTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATc                                                     <  1:132903/141‑1 (MQ=255)
                                                                            tCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAACCGACAAATGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCg                                                    >  1:380348/1‑141 (MQ=255)
                                                                                      aCCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAGGTGTAACCGCAAATACTGAAATCGCTATTCATGa                                          >  1:91902/1‑141 (MQ=255)
                                                                                          aaGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCt                                      <  1:413897/141‑1 (MQ=255)
                                                                                              aggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTACAAATCAAAGTGTAACCGCAAATACTGAAAGCGCTATTCATGATTCTAata                                  <  2:91096/141‑1 (MQ=255)
                                                                                                 aaGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTa                               >  2:25322/1‑141 (MQ=255)
                                                                                                              aTTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAATCTAATATTAAACTATATGaacc                  >  2:126570/1‑139 (MQ=255)
                                                                                                               ttAAAATCGTTAGGTCTTAAAGATGATACGATTGAACCAGTACATAAGAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCGATTCATGATTCTAATATTAAACTATATGAATCt                 >  1:435768/1‑141 (MQ=255)
                                                                                                                         tAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTa       >  1:188077/1‑141 (MQ=255)
                                                                                                                          aGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTaa      >  2:162903/1‑141 (MQ=255)
                                                                                                                            gTCTTAAAGATCATACGATTGAAAAAGTATATAATAAGCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGc    <  2:352335/141‑1 (MQ=255)
                                                                                                                             tCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCa   <  1:270771/141‑1 (MQ=255)
                                                                                                                              cTTAAAGATGATACGATTGAAAAAGTATATAGTAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGACATTAAGCaa  >  1:113878/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                  
GGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAA  >  CP000730/1233368‑1233634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑