Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,761,8890CA100.0% 180.1 / ‑6.5 55D388Y (GAT→TAT) folCtetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (24/29);  total (24/31)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761754‑1762026
                                                                                                                                       |                                                                                                                                         
cttcATGGCTATTTTAGTTTTAGTTTTCTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACGACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                      <  1:274655/141‑1 (MQ=255)
 ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATACTTTTTTATAAATTCAAACTAGTCATATACTaa                                                                                                                                     >  2:487858/1‑141 (MQ=255)
    aTGGCTTCTTTAGTTTTTGTTTTATAATGCTTCAAAGTCTTATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTg                                                                                                                                  <  2:378643/141‑1 (MQ=255)
         tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                             <  1:628289/141‑1 (MQ=255)
           ttttagttttagTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtca                                                                                                                           <  1:102387/141‑1 (MQ=255)
            tttagttttagtTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcat                                                                                                                          <  1:487341/141‑1 (MQ=255)
              tatttttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatca                                                                                                                        <  2:689426/138‑1 (MQ=255)
              tagttttagtttTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatca                                                                                                                        <  2:324063/141‑1 (MQ=255)
                 ttttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAttt                                                                                                                     <  1:75115/141‑1 (MQ=255)
                 ttttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAttt                                                                                                                     <  1:437944/141‑1 (MQ=255)
                 tttcatttttaTAATGATTCAAAATCAAATTGTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAttt                                                                                                                     <  1:154652/135‑1 (MQ=255)
                  tttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTCTTTCATCAtttt                                                                                                                    >  1:408395/1‑141 (MQ=255)
                           ataATGCTTCAAAGTTTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTCCAAtactac                                                                                                           <  2:540896/141‑1 (MQ=255)
                              aTGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActaccta                                                                                                        >  1:359596/1‑141 (MQ=255)
                                   tCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAtt                                                                                                   <  1:837552/141‑1 (MQ=255)
                                   tCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAtt                                                                                                   <  2:118116/141‑1 (MQ=255)
                                        gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttcttc                                                                                              >  2:261133/1‑141 (MQ=255)
                                           tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTTTTATTTGTAAGTAATGATAGGCTAATAGTATTTCATCATTTTCAATACTCCGTACACttcttttttt                                                                                           >  2:263615/1‑133 (MQ=255)
                                           tAATGTTGGTTTGACTTCGCTTATGCAATACGGACTACCGGTAAGTACTAATATATCATCTTGATACTTTTTTATAAATTCAACTTGGTCATATACTTATTGTTTTTCATCATTTTCAATACTAACTATAATTTCTTCttt                                                                                           >  2:364634/1‑141 (MQ=255)
                                                      tAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCtt                                                                                >  2:487468/1‑141 (MQ=255)
                                                      tAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCtt                                                                                <  1:331293/141‑1 (MQ=255)
                                                        actttattttttaaaTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTc                                                                              >  2:453870/13‑141 (MQ=255)
                                                        aCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTc                                                                              >  1:58868/1‑141 (MQ=255)
                                                        aCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGATTTc                                                                              >  2:453875/1‑141 (MQ=255)
                                                              cTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                        <  1:849609/141‑1 (MQ=255)
                                                                tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATc                                                                      >  1:692623/1‑141 (MQ=255)
                                                                         cAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                             <  1:821908/141‑1 (MQ=255)
                                                                          aGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGtt                                                            <  1:788731/141‑1 (MQ=255)
                                                                          aGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGtt                                                            <  1:214518/141‑1 (MQ=255)
                                                                            aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                          <  2:222698/141‑1 (MQ=255)
                                                                             cTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCa                                                         >  2:23354/1‑141 (MQ=255)
                                                                               aCCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATa                                                       >  1:154149/1‑141 (MQ=255)
                                                                                 cGGTAATTTCTTATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                     <  1:803395/141‑1 (MQ=255)
                                                                                  ggTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGTAAAATCAAATTCAGTTGCATaaaa                                                    <  1:632236/141‑1 (MQ=255)
                                                                                  ggTAATTACTAATGTATCACCTTGATAATTTTTAATAAATTCAACGTAGTCATCTGCTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCCTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaaa                                                    <  2:474188/141‑1 (MQ=255)
                                                                                       ttAATAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATATCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaataagatt                                               >  1:621833/1‑134 (MQ=255)
                                                                                        tACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATg                                              <  1:759547/141‑1 (MQ=255)
                                                                                           tAATGTATCACCTTTATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACTATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcgc                                           <  2:562594/141‑1 (MQ=255)
                                                                                           tAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcgc                                           <  2:619005/141‑1 (MQ=255)
                                                                                           gaaTGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcgc                                           <  1:250282/140‑1 (MQ=255)
                                                                                             aTGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAACCGTATGCGCaa                                         >  2:46528/1‑141 (MQ=255)
                                                                                               gTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAAtt                                       >  1:750540/1‑141 (MQ=255)
                                                                                                tATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTa                                      <  2:154149/141‑1 (MQ=255)
                                                                                                   cACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAAc                                   <  1:261133/141‑1 (MQ=255)
                                                                                                    aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACt                                  >  1:796431/1‑141 (MQ=255)
                                                                                                        tGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGAGCAATTCTACTTaaa                              >  2:603575/1‑141 (MQ=255)
                                                                                                           tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCCGTTGCATAAAACGTATGCGCAATTAAACTTCACTGt                           >  1:351688/1‑141 (MQ=255)
                                                                                                            aaTTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGtt                          <  1:257354/141‑1 (MQ=255)
                                                                                                                 tttATAAATTCAACGTAGTCATTTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGAcc                     <  2:768115/141‑1 (MQ=255)
                                                                                                                        aTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGt              <  1:487468/141‑1 (MQ=255)
                                                                                                                             atgTAGTCATATACTAATTGTATTTCATCATTTTGAATACTACCTACAATTTATAAATTTCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAAGTATGCGCAATTAAACTTGAATGTTTGACCATCACGTTaaac         >  1:163111/3‑139 (MQ=255)
                                                                                                                             aCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATc         >  1:788911/1‑141 (MQ=255)
                                                                                                                              cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATTAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        >  1:196395/1‑141 (MQ=255)
                                                                                                                              cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg        >  2:520724/1‑141 (MQ=255)
                                                                                                                                tAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTGTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt      >  1:575010/1‑141 (MQ=255)
                                                                                                                                    cATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTc  <  2:428184/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
CTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761754‑1762026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑