Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 290.6 / NA 86V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (44/42);  total (44/42)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTT  >  CP000730/1963630‑1963905
                                                                                                                                          |                                                                                                                                          
tACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCGTTAAATGCCATCATTTGACTTAAATCTCTACTTACTATCGATACTAACTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGAt                                                                                                                                          <  2:263878/141‑1 (MQ=255)
 aCTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATa                                                                                                                                         >  2:311815/1‑141 (MQ=255)
       cgtaaaaCGCTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAACCGATACTAATTCATTATCTGTATGCTCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  2:96586/137‑1 (MQ=255)
       cGGTAAACGATTGACAATTTTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  2:98247/141‑1 (MQ=255)
       cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCCCg                                                                                                                                   >  1:687573/1‑141 (MQ=255)
       cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  1:699453/141‑1 (MQ=255)
       cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  1:730063/141‑1 (MQ=255)
       cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  2:740234/141‑1 (MQ=255)
       cGGCAAACGATTGACAATTGTAAATTCAGGAGCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCCATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                   <  1:328897/141‑1 (MQ=255)
             aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaa                                                                                                                             >  1:668444/1‑141 (MQ=255)
               gATTGACAATTGTAAATTCAGGATCACCCTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaaaa                                                                                                                           <  1:480670/141‑1 (MQ=255)
                  tGACAATTGTGAATTCAGGAGCACCGTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTCGATACGCACGAATCAAAacct                                                                                                                        >  2:393124/1‑141 (MQ=255)
                  tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                        >  1:711295/1‑141 (MQ=255)
                  tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATAATCTCTATGATCATCAACCACAGAATCACCTGGTTTACCTAGATACGCACGAATCAAAccct                                                                                                                        >  2:287529/1‑141 (MQ=255)
                    gtgaTTGTATATTCAGGAGCACCGTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacctta                                                                                                                      <  2:601381/138‑1 (MQ=255)
                    aCAATTTTAAATTCATGATCACCTTTAAATGTCATTATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCGAGCGCAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacctta                                                                                                                      <  2:694534/141‑1 (MQ=255)
                    aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacctta                                                                                                                      <  2:456774/141‑1 (MQ=255)
                        ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacctt                                                                                                                  <  1:509845/141‑1 (MQ=255)
                             aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATGCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAAACTTACCTTCCGgt                                                                                                             >  1:782677/1‑141 (MQ=255)
                                 cAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACTAAACTACCCATAGGTTTACCTCGCTACGAGAAAATCAAAAACTTACATTGCGGTgtag                                                                                                         >  1:183749/1‑141 (MQ=255)
                                      ttagcTTTAAATGGCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGCATCTAAACCTTACCTTCCGGTGTAGTAAAt                                                                                                    <  2:173428/137‑1 (MQ=255)
                                      tCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAAt                                                                                                    <  1:475667/141‑1 (MQ=255)
                                             tAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTAGGATCATCAACCACAGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGGGTAGAAAAGGGACAtt                                                                                             >  1:256405/1‑141 (MQ=255)
                                                aTGTCGTCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttt                                                                                          <  1:702845/141‑1 (MQ=255)
                                                aTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttt                                                                                          <  1:266779/141‑1 (MQ=255)
                                                  ttcatcaTTTGACTGAAATCTCTACGTAAAATCGATACTAATTCATTATCTGTATGACCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                        <  2:417433/140‑1 (MQ=255)
                                                  gTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                        <  1:445412/141‑1 (MQ=255)
                                                  gTCAGCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                        <  1:225591/141‑1 (MQ=255)
                                                               ttAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCCACCAAAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTGTCGATGTCCAAGTAc                                                                           >  1:68723/1‑141 (MQ=255)
                                                                 aaaTCTCTTCGTACAATCGATTCTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAt                                                                         <  1:335543/141‑1 (MQ=255)
                                                                 aaaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAt                                                                         >  2:722113/1‑141 (MQ=255)
                                                                  aaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATg                                                                        >  1:444562/1‑141 (MQ=255)
                                                                  aaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTAGATGTCCAAGTACATg                                                                        <  2:800292/141‑1 (MQ=255)
                                                                        caCGTACAATCGATACTAATTCATTATCTGCATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa                                                                  <  1:495432/140‑1 (MQ=255)
                                                                           gTACCATCGATACTAATTCATTATCTGTATGAGCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGCACATGCGGTAATGt                                                               <  2:549296/141‑1 (MQ=255)
                                                                           gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt                                                               >  1:169055/1‑141 (MQ=255)
                                                                           gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt                                                               <  2:187880/141‑1 (MQ=255)
                                                                           gTACAATCGATACTAATTCAGTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAGCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt                                                               <  2:417382/141‑1 (MQ=255)
                                                                             acacTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCt                                                             >  2:496370/1‑141 (MQ=255)
                                                                             aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTGCATGCGGTAATGTCt                                                             >  1:324822/1‑141 (MQ=255)
                                                                             aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAACTACATGCGGTAATGTCt                                                             >  2:786116/1‑141 (MQ=255)
                                                                                aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                          >  1:144455/1‑141 (MQ=255)
                                                                                 tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTATACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCTTTTTTTAATTACATGCGGTAATGTCTGTAt                                                         >  2:595408/1‑141 (MQ=255)
                                                                                   gctACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATTCTCACGAATCAAAACCTTACCTTCCGGTATATTAACTCGCCATTTTTTTGATTTCCAAGTACATTCGGTAGTGTCTGTATCa                                                       >  2:316345/3‑141 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       >  2:629524/1‑141 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       >  2:682048/1‑141 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       <  2:407233/141‑1 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       >  1:687626/1‑141 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       >  2:502463/1‑141 (MQ=255)
                                                                                   gATACTAATTCATTATCTGTATGATCATCAACCACAGTAGCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATcc                                                       >  2:68687/1‑140 (MQ=255)
                                                                                   caTACTAATTCACTATCTGTATGATCATCAACCACATTATCACCTGGTTTACCTAGATACGGACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                       <  2:44565/140‑1 (MQ=255)
                                                                                       ttcattcattcTCTGTATGATCATCAACCACAGTATCACCTGGTTTCCCTAGATCCGCACGACTCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCg                                                  <  1:397714/138‑1 (MQ=255)
                                                                                       gtAATTCATTCTCTGTATTATCATCAACCACAGTATCCCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCg                                                  <  1:397762/140‑1 (MQ=255)
                                                                                       cTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCg                                                  >  1:282758/1‑141 (MQ=255)
                                                                                        tAATTCCTTATCTGTATGACCAGCAACCACAGTATCACCTTTTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCTATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGt                                                 <  2:21944/141‑1 (MQ=255)
                                                                                        aaaatCATTATCTGTATGATCATCAACCACAGAATCAACTCGTTTACATAGATCCACACGAATAAAAACCTTACCTTCGTGTGTAGTATATTGCCAGTTTTCCGGTCGTTTAGTCCATGCGGTAATGTCTGAATCACTCGt                                                 <  2:775533/137‑1 (MQ=255)
                                                                                              aTTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGc                                           <  2:24782/141‑1 (MQ=255)
                                                                                               ttATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGACACGCACGAATCGAAACCTTACCTTCCGATGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATTCGGTAATGTCTGTATCACTCGTTCTCGCa                                          >  1:321127/1‑141 (MQ=255)
                                                                                                tATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa                                         <  1:478170/141‑1 (MQ=255)
                                                                                                tATCTGGATGATCATCAACCACAGTATCACCTGGTTTACCTAGACACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATTGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa                                         <  1:38272/141‑1 (MQ=255)
                                                                                                 aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCCCTCGTTCTCGCAAt                                        >  2:417997/1‑141 (MQ=255)
                                                                                                    tGTATGATCACCAACCACGGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGGACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc                                     <  2:1022/141‑1 (MQ=255)
                                                                                                       aTGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCCCTCGTTCTCGCAATTACGaa                                  >  2:7038/1‑141 (MQ=255)
                                                                                                       aTGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGaa                                  <  1:740909/141‑1 (MQ=255)
                                                                                                       aTGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGaa                                  >  2:688441/1‑141 (MQ=255)
                                                                                                            catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCCCCCGTTCTCGCAATTACGAAGCCAg                             >  2:12789/1‑141 (MQ=255)
                                                                                                            catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                             >  2:761790/1‑141 (MQ=255)
                                                                                                            catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTAGCACTCGTTCTCGCAATTACGAGGCCAg                             >  1:339918/1‑141 (MQ=255)
                                                                                                            cagcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTTCATATCTTGCTCGCAATTACGAAGCCAg                             <  1:595408/138‑1 (MQ=255)
                                                                                                             atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                            >  2:720488/1‑141 (MQ=255)
                                                                                                              tcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTa                           >  1:636978/1‑141 (MQ=255)
                                                                                                                aaCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAcc                         >  2:794186/1‑141 (MQ=255)
                                                                                                                  ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCTTTGTTTATTTACATGCGGAAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat                       >  2:539103/1‑141 (MQ=255)
                                                                                                                  ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat                       >  2:679287/1‑141 (MQ=255)
                                                                                                                   cacaGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatc                      <  1:776807/141‑1 (MQ=255)
                                                                                                                      aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg                   <  2:441693/141‑1 (MQ=255)
                                                                                                                           cACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCATTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt              <  1:429109/141‑1 (MQ=255)
                                                                                                                             ccTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatccagggaaata            >  1:277930/1‑131 (MQ=255)
                                                                                                                             ccTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACCCGTTCTCGCAATTACGAAGCCAGTACCACCATGGGCAttt            >  2:21779/1‑141 (MQ=255)
                                                                                                                             ccTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTATGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTGttt            >  2:93789/1‑141 (MQ=255)
                                                                                                                                    tACCTAGATACTCACGAAGCAAAACCTTACCTTCCGTTATTGTAAATTGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATGAATCGTTGCCGGAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGt     <  2:333065/141‑1 (MQ=255)
                                                                                                                                    tACCTAGATACGCCCGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGt     <  2:68246/141‑1 (MQ=255)
                                                                                                                                      ccTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTTCAATTACATGCGGTAAGGTCTGTATCACTCGTTCTCGCAATTACAAAGCCAGTACAATCATTGGTATTTTCAAttgct   >  2:618920/1‑137 (MQ=255)
                                                                                                                                      ccTAGATACGCACGAATCAAAACCTTACCTGCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCt   >  1:239888/1‑141 (MQ=255)
                                                                                                                                      ccTAGATACGCACGAATCAAAACATTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGCAGCCAGTACCATCATGGGTATTTTCAATGTCt   >  2:319656/1‑141 (MQ=255)
                                                                                                                                       cTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCtt  >  2:85829/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTT  >  CP000730/1963630‑1963905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: