Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 667,735 A→C 5.4% intergenic (+767/‑281) USA300HOU_0609 → / → adh phosphohydrolase/alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730667,7350AC 5.4% 209.2 / 4.4 74intergenic (+767/‑281)USA300HOU_0609/adhphosphohydrolase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (24/46);  new base C (2/2);  total (26/48)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

AAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATCTAACTA  >  CP000730/667596‑667875
                                                                                                                                           |                                                                                                                                            
aagtaatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  2:352541/138‑1 (MQ=255)
aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  2:237769/141‑1 (MQ=255)
aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  1:440870/141‑1 (MQ=255)
aaaTAATGTCTTAGATTGAGTGGAAGTTTTTTTAATTTTTTTTAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag                                                                                                                                             <  1:368195/141‑1 (MQ=255)
      tGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGaa                                                                                                                                       <  1:686622/141‑1 (MQ=255)
      tGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGaa                                                                                                                                       <  1:509881/141‑1 (MQ=255)
            aGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAag                                                                                                                                 >  1:231663/1‑141 (MQ=255)
            aGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACAATATAATAAGAGTGAAAATaaa                                                                                                                                 >  2:117653/1‑140 (MQ=255)
               tttattgGGAGTTTTTTCAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGt                                                                                                                              <  2:140532/138‑1 (MQ=255)
                   ttgGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATATGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATAATTTGTGGTTTAATTCACATTATAATAAGAGTAAAGATAAGAGtatta                                                                                                                          >  2:226860/1‑141 (MQ=255)
                     gggAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATtata                                                                                                                        >  1:27281/1‑141 (MQ=255)
                          tttttttAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTa                                                                                                                   >  2:402868/1‑141 (MQ=255)
                             ttttAATTTTTTANAAAATAAATTAATCTCNAATTAATAAAAAATCTGAATAACTGACAAATTTTTTTGATCATCGCTATATACTTTGTGAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCt                                                                                                                <  1:55763/141‑1 (MQ=255)
                              tttAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCtt                                                                                                               <  2:674606/141‑1 (MQ=255)
                                   tttttttGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTtaaat                                                                                                          >  1:706721/1‑141 (MQ=255)
                                   tttttttGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTtaaat                                                                                                          >  1:86907/1‑141 (MQ=255)
                                                aaattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTTATTTTTGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGaa                                                                                             >  2:29355/1‑141 (MQ=255)
                                                aaattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGaa                                                                                             >  1:770838/1‑141 (MQ=255)
                                                 aattaattTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  2:489143/141‑1 (MQ=255)
                                                 aattaaTCTGTATTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATATTAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  1:543440/141‑1 (MQ=255)
                                                 aattaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  1:320556/141‑1 (MQ=255)
                                                 aattaaACTGTAATTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGCAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAg                                                                                            <  2:433025/141‑1 (MQ=255)
                                                   ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          <  1:463688/141‑1 (MQ=255)
                                                   ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          <  1:270159/141‑1 (MQ=255)
                                                   ttaaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTa                                                                                          >  1:785709/1‑141 (MQ=255)
                                                    taaTCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTaa                                                                                         >  1:470407/1‑141 (MQ=255)
                                                       tCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTaaaaa                                                                                      >  2:178997/1‑141 (MQ=255)
                                                        cTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAAt                                                                                     <  2:756818/141‑1 (MQ=255)
                                                                  taaAGAATTTGAATAACTGACACATGTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTCTAATAAGAGTGAAGATACGAGTATTATCAATTATCTTTAATTAAATATATGTGACGGAGAAGTTACACGtttg                                                                           >  2:430083/1‑139 (MQ=255)
                                                                  taaAAAATTTGAATAACTGACACATTTTCTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAg                                                                           >  2:91639/1‑141 (MQ=255)
                                                                     aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:706721/141‑1 (MQ=255)
                                                                     aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATACGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:86907/141‑1 (MQ=255)
                                                                     aaaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  2:669374/141‑1 (MQ=255)
                                                                     aaaaGTGGAATAACTGACACATTTTTTCTATCATATCTATATACTTGGGGAATTTATGCACATGATAAGAAGAGTGAAGATAAGATTATTAGAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCat                                                                        <  1:364574/141‑1 (MQ=255)
                                                                      aaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCata                                                                       >  2:208040/1‑141 (MQ=255)
                                                                      aaaTTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCata                                                                       >  1:774066/1‑141 (MQ=255)
                                                                               tAACTGACACATTTTTTTGATTATAGCTATATACGTTGGTAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATg                                                              <  2:631546/141‑1 (MQ=255)
                                                                               tAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATg                                                              >  2:231123/1‑141 (MQ=255)
                                                                                aaCTGACATATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGt                                                             <  2:697320/141‑1 (MQ=255)
                                                                                aaCTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGt                                                             <  2:119257/141‑1 (MQ=255)
                                                                                aaCTAACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAACATTACACGTTAGCATATCGATTATGt                                                             <  2:502642/141‑1 (MQ=255)
                                                                                 aCTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATATTCAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATATATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTc                                                            <  2:431423/141‑1 (MQ=255)
                                                                                   tGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCTATTCTTTCAt                                                          >  2:130912/1‑141 (MQ=255)
                                                                                   tGACACATTTTTTTGATCATAGATATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  2:750880/141‑1 (MQ=255)
                                                                                   tGACAAATTTTTTTGATCATAGCTATAAACTTTTTGAATTAATTCAAATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  1:171372/141‑1 (MQ=255)
                                                                                   tGAAACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCAt                                                          <  2:200654/141‑1 (MQ=255)
                                                                                    gACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATACATTATCTTTTAATAAATCTATGTGCAGTAAACATTACACGTTAGCATATCGATTATGTCAtt                                                         <  2:799299/141‑1 (MQ=255)
                                                                                       ataTTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCt                                                      <  2:79708/139‑1 (MQ=255)
                                                                                        caTTTTTTTGATCAGAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGCAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCtt                                                     <  2:491455/141‑1 (MQ=255)
                                                                                        caTTTTTTTGAGCATAGCTATATATTTTGTTAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCtt                                                     <  2:36476/141‑1 (MQ=255)
                                                                                            tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATCTTCAAACTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 <  1:383059/141‑1 (MQ=255)
                                                                                            tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 >  2:227364/1‑141 (MQ=255)
                                                                                            tttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTaa                                                 <  2:351213/141‑1 (MQ=255)
                                                                                             ttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAAc                                                <  1:259425/141‑1 (MQ=255)
                                                                                             ttttGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAAc                                                <  1:92752/141‑1 (MQ=255)
                                                                                                tGATCATAGCTATATACTTAGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATCTGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACaat                                             >  1:454159/1‑139 (MQ=255)
                                                                                                       aGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTa                                      <  1:82050/141‑1 (MQ=255)
                                                                                                          tatataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAgga                                   >  1:341422/1‑140 (MQ=255)
                                                                                                           atataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGa                                  >  1:153963/1‑141 (MQ=255)
                                                                                                            tataCTTTGTGTATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAACTTATCTTTAAATAAATATATGTGAAGTAAAAACTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGaa                                 <  1:218584/141‑1 (MQ=255)
                                                                                                            tataCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGaa                                 <  2:241094/141‑1 (MQ=255)
                                                                                                              taCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAAc                               >  2:542174/1‑141 (MQ=255)
                                                                                                              taCTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATCATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAAc                               <  1:402868/141‑1 (MQ=255)
                                                                                                                cTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTGTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAAGGAAACGt                             >  1:253265/1‑141 (MQ=255)
                                                                                                                 tttGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGtt                            <  1:31428/141‑1 (MQ=255)
                                                                                                                  ttGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTa                           <  2:143950/141‑1 (MQ=255)
                                                                                                                     tgAATTAATTCAGATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTaaaa                        <  2:455504/141‑1 (MQ=255)
                                                                                                                     tgAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTaaaa                        <  2:27281/141‑1 (MQ=255)
                                                                                                                      gAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAg                       <  1:347050/141‑1 (MQ=255)
                                                                                                                        attaattCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACAGGGTAGCATATCGATTATGTCATTTCTTTTAAAATATTAACTAGGGGAACGTGAAAAGtg                     >  1:145988/1‑140 (MQ=255)
                                                                                                                        attaattCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGtt                     >  1:404150/1‑141 (MQ=255)
                                                                                                                             ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  1:231123/141‑1 (MQ=255)
                                                                                                                             ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  2:231663/141‑1 (MQ=255)
                                                                                                                             ttCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACgg                <  1:117653/141‑1 (MQ=255)
                                                                                                                               cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATCTCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  2:799254/141‑1 (MQ=255)
                                                                                                                               cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  1:661073/141‑1 (MQ=255)
                                                                                                                               cacaTTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGtt              <  1:29355/141‑1 (MQ=255)
                                                                                                                                    tataATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGatat         <  1:705675/141‑1 (MQ=255)
                                                                                                                                      taataaGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATct       <  1:91639/141‑1 (MQ=255)
                                                                                                                                      aaataaGAGTAAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATct       <  1:404062/140‑1 (MQ=255)
                                                                                                                                       aataaGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATcta      >  2:788173/1‑141 (MQ=255)
                                                                                                                                        ataaGAGTGAAGATAAGAGTATTGTAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaa     >  2:372738/1‑141 (MQ=255)
                                                                                                                                          aaGAGTGAAGAGAAGAGTATTATAAATTATCTTTAAATAAATAGATGTGAAGTAAAAAGTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaact   <  1:769558/141‑1 (MQ=255)
                                                                                                                                           agagGGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATctaacta  <  1:399471/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAGATAAGAGTATTATAAATTATCTTTAAATAAATATATGTGAAGTAAAAATTACACGTTAGCATATCGATTATGTCATTTCTTTTAACATATTAACTAGGGAAACGTTAAAAGTTAACGGTTGATATCTAACTA  >  CP000730/667596‑667875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑