Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 207.9 / ‑6.5 70V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (33/32);  minor base T (1/3);  total (34/36)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCT  >  CP000730/1963633‑1963904
                                                                                                                                       |                                                                                                                                        
tttactgCAAAAGATTGACAATTTTAAATTCAGGATCCCCTTTAAACGTCATCATTTGACTTAAATCTCTACGTACAACCGATACTAACTCATCATCTGTATGATCATAAACCACAGTCTCACCTTGTTTACCTATATAc                                                                                                                                      <  2:146441/134‑1 (MQ=255)
    cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCAc                                                                                                                                  <  1:685949/140‑1 (MQ=255)
      gCACACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATCATCTGTCTGATCCTCAACCCCAGTATCACCTCGTTTAGCTATATACGCACGa                                                                                                                                >  2:333259/1‑140 (MQ=255)
        aaaCGATTGACAATTGTAACTTTAGGATCACCTTTAAATGGCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAt                                                                                                                              <  2:647099/140‑1 (MQ=255)
                     ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                                 <  2:90629/140‑1 (MQ=255)
                     ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                                 >  1:637767/1‑140 (MQ=255)
                     ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                                 <  1:476673/140‑1 (MQ=255)
                     ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                                 <  2:39510/140‑1 (MQ=255)
                     ttGTAAATTCAGGATCAAATTCGTGGATCTTCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttccct                                                                                                                 >  2:162955/1‑140 (MQ=255)
                       gTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc                                                                                                               <  2:384907/140‑1 (MQ=255)
                       gTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc                                                                                                               <  1:46016/140‑1 (MQ=255)
                       gTAAATTCAGAATAAGCTTCAAATGACACCATTTTATCTAAAGCTTTACGTAAATTTTATACTAATTCATTATCTGTATTATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc                                                                                                               <  1:156547/140‑1 (MQ=255)
                        tAAATCCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAACCCTTCCCTAcc                                                                                                              <  2:107631/140‑1 (MQ=255)
                          aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCCTCAACCACAGTATCACCTGGTTTAAATCGATACACTCGAATAAAAacccttgcctcggt                                                                                                            >  2:717364/1‑129 (MQ=255)
                          aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg                                                                                                            >  2:72027/1‑140 (MQ=255)
                             tCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                         <  1:630920/140‑1 (MQ=255)
                                 gATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTgtagta                                                                                                     <  2:553296/140‑1 (MQ=255)
                                  aTCACCTTTAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTaa                                                                                                    <  1:579042/140‑1 (MQ=255)
                                  aTCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTaa                                                                                                    <  1:128749/140‑1 (MQ=255)
                                   ctaCCTTTAAAGGTCATCTTTTGACTTAAGTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATGACCTGGTTTACCGATATGCGCACGAATCAAAACCTTACCTTCCGGTGTAGTaaa                                                                                                   <  2:378502/138‑1 (MQ=255)
                                   ccACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTaaa                                                                                                   <  2:407602/139‑1 (MQ=255)
                                      ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                                >  1:715445/1‑140 (MQ=255)
                                      ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                                >  1:498848/1‑140 (MQ=255)
                                         ttAAATGTCTTCATTTGACGTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACGGTATCACCTGGTTGACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGGAAATGGCCa                                                                                             <  2:489774/140‑1 (MQ=255)
                                          tAAATGTCATCATTTGACTTAAAGCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt                                                                                            >  1:368750/1‑140 (MQ=255)
                                             aTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATGATAAACCACAGTATCACCTGGTTTACCTAGATACGCACGAACCAAAACCTTCCCTTCCGGTGTTTACAATGCCCAtttt                                                                                         >  2:358105/1‑140 (MQ=255)
                                                ttatcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATTACCTGGTTTATCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                      <  1:390551/138‑1 (MQ=255)
                                                tcatcaTTTGACTTAAATCTCTTCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCTCTGTATCACCTGGTTTACCTAAATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                      <  1:750622/140‑1 (MQ=255)
                                                  atcatTTGACTTAAATCTCTTCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                    <  2:334497/140‑1 (MQ=255)
                                                         gACTTAAATCTCTAAGTAGAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa                                                                             >  2:541281/1‑140 (MQ=255)
                                                                  ctctACCTCCAATCGATCCTAAGTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg                                                                    <  1:454027/140‑1 (MQ=255)
                                                                          acaactgATACTAATTCATTATCGCTATGTTCATCGACCACTGTATCACCTGGTGTACCGAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAACTGGGCATTTTTCCGATGTCCAAGTACATGCGGTAATGTc                                                            <  2:423399/134‑1 (MQ=255)
                                                                          aCAATCGATACTAATTCATTATCTGTATGATTATCAACCACACGATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTc                                                            <  1:394387/140‑1 (MQ=255)
                                                                           catttgtagatAATTCATTATCTGATTGATCATCAACCACATTATCACCTGGTTTACCTAGATACGCACGAATCAAAAACTTACCTTACGGTGTAGTAAATGGCCATTTTTTCGATGTTCAAGTACATGCGGTAATGTCt                                                           <  2:743817/130‑1 (MQ=255)
                                                                              ttgATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAGCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                        <  2:710489/138‑1 (MQ=255)
                                                                                gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAGTGGCCATGTTTTCGATGTTCAAGTACATGCGGTAATGTCTGTATc                                                      >  2:45395/1‑140 (MQ=255)
                                                                                gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGGGTAGGAAATGGCCGTTTTTTCGATTCCCAACTACATCGGGTAATTCCTGttca                                                      >  2:10444/1‑137 (MQ=255)
                                                                                gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCAGGTTTACCTAGAGACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTACATGCGTTAATGTCTGTATc                                                      >  2:123902/1‑140 (MQ=255)
                                                                                 aTACTAATTCATTATCTGTATGTTCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCACGTAAATGCGGTAATGTCTGTATCa                                                     <  1:152140/140‑1 (MQ=255)
                                                                                 aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa                                                     <  1:562550/140‑1 (MQ=255)
                                                                                  tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                    >  1:72970/1‑140 (MQ=255)
                                                                                      aaTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTCCGGTTGTGGTAAATGGCCATTTTTTCGATGTCCAAGTACATCCGGTAATGTCTGTCACACTCGt                                                >  2:269551/1‑140 (MQ=255)
                                                                                            ttATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATTGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGc                                          <  1:10007/140‑1 (MQ=255)
                                                                                              aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCAGTATCACTCGTTCTCGCaa                                        >  1:185138/1‑140 (MQ=255)
                                                                                                cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCCCTCGTTCTCGCAAtt                                      >  1:51357/1‑140 (MQ=255)
                                                                                                 tGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCACGTACATGCGGTAAGGTCTGTATCACTCGTTCTCGCCAttt                                     >  2:443051/1‑139 (MQ=255)
                                                                                                           tcaACCTCAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                           >  2:560432/1‑140 (MQ=255)
                                                                                                           tcaACCACATTATTACCTGGTTTACTTATATACGCACGAATCAAAACCTTACTTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTATATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                           <  2:236659/140‑1 (MQ=255)
                                                                                                           tcaACCACAGTATCCCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                           <  1:554685/140‑1 (MQ=255)
                                                                                                            caACCCTAGTATCACCTGTTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATCACAAAGTACATGCGGTAATGTCTGTATCACCCGTTCTCGCAATTACGAATCCAGTa                          <  2:193999/140‑1 (MQ=255)
                                                                                                            caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTTTAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCCGTa                          >  2:726884/1‑140 (MQ=255)
                                                                                                            caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTAGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAGGCCAGTa                          >  2:619138/1‑140 (MQ=255)
                                                                                                            caACCACAGTATCACATGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTa                          >  2:195774/1‑140 (MQ=255)
                                                                                                             aacaacAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCGTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTTTATCACTCGTTTTCGCCATTACGAAGCCAGTAc                         <  2:49099/140‑1 (MQ=255)
                                                                                                             aaCCACAGTTTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc                         <  2:278101/140‑1 (MQ=255)
                                                                                                             aaCCACAGTATCACCTGGTTTACCTAGATACGCCCGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTCTCACTCGTTCTCGCAATTACGAAGCCAGTAc                         <  2:427135/140‑1 (MQ=255)
                                                                                                             aaCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTTGCAATTACGAAGGCAGTaa                         >  1:622477/1‑139 (MQ=255)
                                                                                                             aaCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGAGGTAATGTATGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc                         >  1:4393/1‑140 (MQ=255)
                                                                                                             aaCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCGTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc                         <  2:382546/140‑1 (MQ=255)
                                                                                                                    gTATCACCTGGTTTACCTAGATACGTACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTACCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg                  <  1:753761/140‑1 (MQ=255)
                                                                                                                     tATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTCAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATgg                 >  2:137472/1‑140 (MQ=255)
                                                                                                                      aTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAATCGTTCTAGCAATTACGAAGCCAGTACACTAATggg                >  2:563443/1‑140 (MQ=255)
                                                                                                                       tCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTACATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAACATCGTGGGt               >  1:53918/1‑140 (MQ=255)
                                                                                                                       tCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGt               >  1:442393/1‑140 (MQ=255)
                                                                                                                             ggTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc         >  2:722297/1‑140 (MQ=255)
                                                                                                                                ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTTTTTGTACATGCGGTAATGTCTGTATCACTAGTTTTCGCAATTGTGCAGCCAGTACCCTTATCGGTCTTTTCAAt      >  2:676074/1‑140 (MQ=255)
                                                                                                                                ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTaaac      >  2:602475/1‑139 (MQ=255)
                                                                                                                                  aCCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAGTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCCTGGGTATTTTCCATGt    >  2:256485/1‑140 (MQ=255)
                                                                                                                                  aCCTAGATACGCACGAATCAAAACCTTACCTTACGGTGTAGTAAATGGCCATTTTTTCGCAGTCCAAGTAAATGCGGTAATGTCAGAAACACTAGTTCACCCCATTAAGAGGCCAGTACCATCATGTGTaatctgaattt    >  2:210029/1‑130 (MQ=255)
                                                                                                                                    cTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAACCCAGTACCATAATGGGTATTTTCAATTTCt  >  1:312434/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCT  >  CP000730/1963633‑1963904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: