Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 163.4 / NA 50A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/22);  total (28/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646052‑1646324
                                                                                                                                         |                                                                                                                                       
ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGcctcct                                                                                                                                       <  1:305693/140‑1 (MQ=255)
ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTCGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGcctcct                                                                                                                                       <  2:649159/140‑1 (MQ=255)
  ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                     <  1:32454/140‑1 (MQ=255)
  ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTACAGTCATGCCTCCttt                                                                                                                                     <  1:451334/140‑1 (MQ=255)
   tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCCTATGTTCCAAACATTTTGGTCTTTCAAAATCCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                    <  1:178164/140‑1 (MQ=255)
    aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTTCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                   <  1:190779/140‑1 (MQ=255)
    aTCACTTCCAACACCATGTGGTAATGCACCTCTATTACCAGATGCTACAATCGTATCGAATTATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                   <  1:264325/140‑1 (MQ=255)
     gcACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCATTAATTCTTTTTCAGTCATGCTTCGTTTTac                                                                                                                                  <  1:558691/139‑1 (MQ=255)
         ttGCAACACCATGTGGTAATGCCTCCCTAGGACCAGATGATACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                              <  2:568240/140‑1 (MQ=255)
              acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                         >  1:103367/1‑140 (MQ=255)
                 ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAat                                                                                                                      >  1:572752/1‑140 (MQ=255)
                     gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAatatat                                                                                                                  >  1:282525/1‑140 (MQ=255)
                           aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCatat                                                                                                            >  1:70750/1‑140 (MQ=255)
                             gCACCCCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGTACCTAATTCGTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                                                                          <  1:632590/140‑1 (MQ=255)
                                   ctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                    >  2:543859/1‑140 (MQ=255)
                                      tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaaa                                                                                                 >  1:312624/1‑139 (MQ=255)
                                          cAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTATAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCCTATGTTTCATcaacacaa                                                                                             >  1:96157/1‑137 (MQ=255)
                                            gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAtt                                                                                           >  1:554809/1‑140 (MQ=255)
                                            gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAtt                                                                                           >  1:237424/1‑140 (MQ=255)
                                            gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCAGTTTGCTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAATTAAAGTAATATCATATGTTtcatcataattctt                                                                                           >  2:491171/1‑132 (MQ=255)
                                              tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                                                                         <  2:588007/140‑1 (MQ=255)
                                                cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                                                                       >  1:220673/1‑140 (MQ=255)
                                                cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                                                                       >  1:562903/1‑140 (MQ=255)
                                                  aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCCTGCCTCCTTTTACAACAGTCACAATACATTCATCTGTTTCTGCAACAGTTGTAgctgc                                                                                     <  2:636566/140‑1 (MQ=255)
                                                  aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                                                                     <  2:512297/140‑1 (MQ=255)
                                                  aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTATAGCATTTTGCTTTCTAATATCGCCTTTAATTCTTTTTCAGTCATGCATCCTTTTACAACCGTTAAAATATCTTACTCTGTTTCATCACAAATCTTAgccgc                                                                                     >  2:704036/1‑140 (MQ=255)
                                                      tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTTGCCTTTTGCTTTCTAATATTGTCTTTAATTCTTTTTCAGTCATGCCTCCTTATACAACAGTTAAAAGATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                 <  1:75339/140‑1 (MQ=255)
                                                            cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTGTCAGTCATGCCTCCTTTTCCAACCGTTAACATAAGTTCATATGTTTCATCAACAATATGNACTGCTTTTTGAAtt                                                                           >  1:730310/1‑140 (MQ=255)
                                                                tgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                       <  2:310684/140‑1 (MQ=255)
                                                                 gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGc                                                                      <  1:295825/140‑1 (MQ=255)
                                                                       ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                >  1:350121/1‑140 (MQ=255)
                                                                               tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                        >  2:742754/1‑140 (MQ=255)
                                                                                  tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                     >  2:85609/1‑140 (MQ=255)
                                                                                  tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                     >  2:230884/1‑140 (MQ=255)
                                                                                  tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt                                                     <  2:494474/140‑1 (MQ=255)
                                                                                   aaTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                    <  2:584579/140‑1 (MQ=255)
                                                                                        tAGCGTTTTGCCTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCTTCTTTGACGt                                               <  2:515163/140‑1 (MQ=255)
                                                                                        tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                               <  2:463659/140‑1 (MQ=255)
                                                                                        tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                               >  2:468567/1‑140 (MQ=255)
                                                                                          gCATTTTGCTCTCTGATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCTTCAGCATCTTTGACGtct                                             >  2:515062/1‑140 (MQ=255)
                                                                                              tttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCATCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTATTTGAATTAAATCAATTTCGTCAGAATCTTTTGCGACTCTaa                                         >  2:626199/1‑140 (MQ=255)
                                                                                                 gCTTTCTAATAGTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                                      <  2:426665/140‑1 (MQ=255)
                                                                                                       tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                >  1:90135/1‑140 (MQ=255)
                                                                                                       tAATATTGCCTTTAATTCCTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCATCATCTTTGACGTCTCTAATtttattta                                >  1:194162/1‑140 (MQ=255)
                                                                                                           atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTTAAGAAATTTCGTCAGCATCTTTACAGTTTGTAATTTTATCtcatgt                            >  2:542120/1‑135 (MQ=255)
                                                                                                           atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                            >  2:542106/1‑140 (MQ=255)
                                                                                                               ccgttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                        <  2:534278/137‑1 (MQ=255)
                                                                                                               ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                        <  1:543859/140‑1 (MQ=255)
                                                                                                               ccTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTa                        <  1:244742/140‑1 (MQ=255)
                                                                                                                           tttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt            >  1:84475/1‑140 (MQ=255)
                                                                                                                               aGTCATGCCTCCATTTACAACAGTTAAAATATATCCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTTGTCAGCATCTTTCACGTCTCCAATTTTCTCTACAGTATTAGGAATGCTTATTAATg        >  2:298303/1‑140 (MQ=255)
                                                                                                                                     gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATATACAGTATTAGAAATGCTTATTAATGATATAc  >  2:293123/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646052‑1646324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑