Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 6.8% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT 6.8% 200.0 / 5.3 76D170D (GAC→GATUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (26/43);  new base T (3/2);  total (31/45)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTC  >  CP000730/110511‑110791
                                                                                                                                            |                                                                                                                                            
aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                              >  2:243887/1‑141 (MQ=255)
 aaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTACAGATGCAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACg                                                                                                                                             <  1:30067/141‑1 (MQ=255)
   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                           <  1:358146/141‑1 (MQ=255)
     aaatCCGTTTAAAATAGTAGATAATAAAAACATTCTAACAAAAGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgaga                                                                                                                                         <  2:379713/139‑1 (MQ=18)
      tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAAATTGAAAAATTATAAAGACGgagaa                                                                                                                                        >  1:367233/1‑140 (MQ=255)
         ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGCCGGAGataag                                                                                                                                     >  2:534857/1‑139 (MQ=255)
         ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAGGGAGATAtt                                                                                                                                     >  1:289468/1‑141 (MQ=255)
          cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                    <  1:5727/141‑1 (MQ=255)
          cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                    <  1:30260/141‑1 (MQ=255)
          cGGTTAAAATGGTCGATAATAAAATCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTATAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACAGAGATAttt                                                                                                                                    >  2:7468/1‑141 (MQ=255)
                   tGGTCGATAATAAAATAATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAAGCGAAAACTTTAAGTTATTTGTTCAATCTGGGGCATTTAAAGATTTGCAAAATTATAAGGTAGGAGATATTTAatcaagtt                                                                                                                           >  1:4852/1‑135 (MQ=14)
                    ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATca                                                                                                                          >  2:173950/1‑140 (MQ=255)
                      tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTATGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                        <  2:655460/141‑1 (MQ=255)
                      gcGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGACAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                        <  2:273923/140‑1 (MQ=255)
                       cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                       >  2:681973/1‑141 (MQ=255)
                        gATAATAAAATCATTACAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                      <  2:346056/141‑1 (MQ=255)
                             taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGAGTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                 >  2:446031/1‑141 (MQ=255)
                             taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                 <  1:364283/141‑1 (MQ=255)
                              aaaaCCATTTCAACAAAAGAAATTAAAAATTCAAATATAAAAAAAGAAATCGAAAACTTTAAGTTCTATGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                <  1:210373/141‑1 (MQ=17)
                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAATCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                               <  2:244062/141‑1 (MQ=255)
                                aaTGATTCCAACAGAAGAAATTAATGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                              <  2:174000/141‑1 (MQ=255)
                                 aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                             >  2:440788/1‑141 (MQ=255)
                                   cATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTa                                                                                                           <  1:446031/141‑1 (MQ=255)
                                       ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                       >  2:588755/1‑141 (MQ=255)
                                         aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                     <  1:487328/141‑1 (MQ=255)
                                         aacaaAAGAAATGAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                     <  1:705225/141‑1 (MQ=255)
                                               aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                               <  1:743079/141‑1 (MQ=255)
                                                  aaTTAAAGATGAAAAAATAAAAAAAGAGATGGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                            >  1:757315/1‑141 (MQ=255)
                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                            >  1:95294/1‑141 (MQ=255)
                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                         <  1:576312/141‑1 (MQ=255)
                                                      aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGTTGCCGAGGTATACGGATAAATATCAAtga                                                                                        >  1:58480/1‑139 (MQ=17)
                                                        aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                      <  1:243887/141‑1 (MQ=255)
                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAAca                                                                                     >  2:188898/1‑140 (MQ=255)
                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAAAGGTGCCGAATTATTCGGCTAAATATCAATTAACt                                                                                     >  2:660524/1‑141 (MQ=255)
                                                               aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                               <  1:693279/141‑1 (MQ=255)
                                                                    aaaaaaaGAAATCGAANACTTTATCGCATTTGTTCAAAATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGATAGATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                          <  1:609917/141‑1 (MQ=14)
                                                                    aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                          >  1:48648/1‑141 (MQ=255)
                                                                    aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                          <  1:22848/141‑1 (MQ=255)
                                                                       aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                       <  1:322096/141‑1 (MQ=255)
                                                                       aaaaGAAATAGAACACTGTAAATCCTTTGTTCAATATGGCGACTTGAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGTTGCCGATTTATTCGGCTAAATATCAATTAACTACTCACGCTTATaa                                                                       <  2:286603/141‑1 (MQ=17)
                                                                             aaTCTAAAATTTTAAGTTCTTTATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGCCTAAATATCAGTTAACTAATGATGATTATAATGTaaa                                                                 <  2:640541/141‑1 (MQ=17)
                                                                             aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                 <  2:349136/141‑1 (MQ=255)
                                                                                cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                              >  1:236833/1‑141 (MQ=255)
                                                                                  aaaaCTTTAAGTGCTTTTTTCAATATGGCGATTTTAAAAATTTGAAAAATTATAAATACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                            <  2:101115/141‑1 (MQ=255)
                                                                                   aaaCTTTAAGTTCTTTGTTCAATTTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGATTTATTCGGCTAAATATCAATTGACTGATGATGATTATAATGTAAAACAAtt                                                           <  2:319114/141‑1 (MQ=255)
                                                                                    actcttAAGTTGTTTGTTCAATGTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                          <  2:719858/137‑1 (MQ=255)
                                                                                     aCTTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAGATTATAAAGACGGCGATATTTCATATAATCCAGAGGTCCCTAGTTAGACTGCAAAAGATCATGTAACTAATGATGATTATAATGTAAAAAAAGTaa                                                         >  2:540045/1‑140 (MQ=17)
                                                                                     aCTTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                         <  2:131767/141‑1 (MQ=255)
                                                                                     aCTTTAATTTCTTTGTTCAATATGGCGACTGTAAAAATTTGAAAAATTATAAAGTCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTTTTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAACACAATTAc                                                         <  2:564997/141‑1 (MQ=17)
                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                       <  1:656237/141‑1 (MQ=255)
                                                                                          aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                    <  1:588755/141‑1 (MQ=255)
                                                                                           aGTTCTTTGTTCAATATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAAAAATACGGaaaa                                                   >  2:217234/1‑141 (MQ=255)
                                                                                                tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGAGATTTCATATAATCCAGAGGTGCCGAGTTGTTCGGCTAAATATCAAGTAACTAATGGTGATAATAATGTAGAACAATTACGCAAAAgatat                                              >  1:295439/1‑141 (MQ=18)
                                                                                                     tCAATAAGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                         <  2:54954/141‑1 (MQ=255)
                                                                                                      cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                        <  1:390977/141‑1 (MQ=255)
                                                                                                            ggCGACATCAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                  <  2:577793/141‑1 (MQ=255)
                                                                                                                 cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                             <  1:566117/141‑1 (MQ=255)
                                                                                                                    tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGCGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCGAAAGATATGAGATACCGACGAGGAAAgg                          >  1:445652/1‑140 (MQ=255)
                                                                                                                    tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGCTATACCGACGAGTAAAGc                          >  2:310038/1‑141 (MQ=255)
                                                                                                                    tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                          >  1:94850/1‑141 (MQ=255)
                                                                                                                    tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACAGACGGGTAAAgt                          >  2:99781/1‑140 (MQ=255)
                                                                                                                     aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                         <  1:559759/129‑1 (MQ=11)
                                                                                                                      aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                        >  1:239402/12‑141 (MQ=11)
                                                                                                                       agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       <  1:665315/131‑1 (MQ=14)
                                                                                                                       agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       >  2:346286/11‑141 (MQ=11)
                                                                                                                       aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGACAGGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       <  1:213404/141‑1 (MQ=255)
                                                                                                                       aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGCGTAGCTTGATGCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       <  1:213415/141‑1 (MQ=21)
                                                                                                                          tttGAAAAATTATCAAGACGGATATATGTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTATTGATGATTATAATGTAAAACAATTACGCAAAAGATATTATATACCGACGAGTAAAGCTCCaaa                    <  2:135044/141‑1 (MQ=18)
                                                                                                                          tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                    <  2:607597/141‑1 (MQ=255)
                                                                                                                          ttggAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                    <  1:227012/138‑1 (MQ=255)
                                                                                                                           ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                   >  1:706356/1‑141 (MQ=255)
                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt               <  1:129626/141‑1 (MQ=255)
                                                                                                                                aaaGTATAAAGACGGGGATATTTCATATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATGAATTAACTAATGATGATTATAATGTAAGAAAATTACGCAAAGGATATGAATTACCGGCGAATAAAGCTCCAAAGtggtt              >  2:70813/1‑137 (MQ=14)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTAGAATGTAAAACAATTACGCAAAAGATATGTTATACCGACGAGTAAAGCTCCAAAGTTATTGt            <  2:579368/141‑1 (MQ=255)
                                                                                                                                      taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa        >  1:350676/1‑141 (MQ=255)
                                                                                                                                        aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGATAAAAg      >  2:328355/1‑141 (MQ=255)
                                                                                                                                         aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTCAAAAGATATGCTATACGTACACGTAAAGCTCCAAAGTTATAGTTAAAAgg     >  2:191006/1‑141 (MQ=17)
                                                                                                                                            cGGAGCTATTTCACATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTc  <  2:393545/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTC  >  CP000730/110511‑110791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: