Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 30.9% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT30.9% 548.4 / inf 496intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (177/164);  new base T (77/76);  total (254/242)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 501 total aligned reads displayed.

AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405
                                                                                                                                            |                                                                                                                                              
                           taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTg                                                                                                                   <  1:332793/135‑1 (MQ=255)
                            taaggaGGTTCTGTTGCAAAGTTGAATTTATAATAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGa                                                                                                                  <  2:706117/136‑1 (MQ=11)
                              atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGt                                                                                                                <  2:532222/138‑1 (MQ=9)
                              atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGt                                                                                                                <  2:354534/138‑1 (MQ=2)
                               ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGtt                                                                                                               <  2:510236/141‑1 (MQ=38)
                                  ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATc                                                                                                            <  1:494547/141‑1 (MQ=35)
                                            cAAAGTTGAATATATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATc                                                                                                  <  2:660882/141‑1 (MQ=12)
                                               agctgcatttagaGTATAATTTGAACAAAAAGGAGCATGCTGTATGAACTATTTCAGATATAAACAATATAACAAGGACGCTATCAATGTAGCTGTTG‑‑GCTACTATCTAAGATATGCAGTGAGTTATCGTGATATATCTGa                                                                                               <  1:193114/129‑1 (MQ=1)
                                                  tGAATCTATAATATAACCTCAGCTAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAGGCTATGCATTCAGCTACCGTGATATAACTGTAat                                                                                            >  2:545202/1‑141 (MQ=11)
                                                     atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                         <  1:605172/138‑1 (MQ=2)
                                                     atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                         >  2:764928/4‑141 (MQ=2)
                                                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  1:760682/140‑1 (MQ=12)
                                                       ttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                       <  2:585809/140‑1 (MQ=17)
                                                        tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg                                                                                      >  1:315741/1‑141 (MQ=17)
                                                         ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                     <  2:206103/141‑1 (MQ=17)
                                                          taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                    >  1:88318/1‑141 (MQ=17)
                                                           aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                   <  1:127680/139‑4 (MQ=12)
                                                              ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                >  2:517665/1‑141 (MQ=17)
                                                                aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtg                                                                              <  2:697321/141‑1 (MQ=32)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                           >  2:643491/1‑141 (MQ=17)
                                                                    ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                          <  1:351245/141‑1 (MQ=17)
                                                                    ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                          <  2:87964/141‑1 (MQ=12)
                                                                    ttAACAAAAAGGAGTCTTCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                          <  2:101093/141‑1 (MQ=255)
                                                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAGAGAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                         <  1:101508/141‑1 (MQ=255)
                                                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                       >  2:494868/1‑141 (MQ=12)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCTGATAGAAACAATTTAACCAGGATTTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGt                                                                    <  2:312002/141‑1 (MQ=255)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                    >  2:276152/1‑141 (MQ=17)
                                                                             aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                                 >  1:658585/1‑141 (MQ=17)
                                                                             aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                                 <  1:11599/141‑1 (MQ=17)
                                                                             aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTCGGGTAAACGAtca                                                                 >  2:717457/1‑141 (MQ=11)
                                                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                             >  1:371353/1‑141 (MQ=12)
                                                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCAtt                                                            >  2:442203/1‑141 (MQ=17)
                                                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCAtt                                                            >  2:120470/1‑141 (MQ=12)
                                                                                    ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCa                                                          >  2:566527/1‑141 (MQ=17)
                                                                                    ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCa                                                          <  2:321617/141‑1 (MQ=17)
                                                                                     tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa                                                         <  1:528089/141‑1 (MQ=17)
                                                                                      ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTACACGTTCATCATTCAAc                                                        <  1:457015/141‑1 (MQ=255)
                                                                                      ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAc                                                        <  2:749250/141‑1 (MQ=12)
                                                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACg                                                       >  2:18146/1‑141 (MQ=17)
                                                                                         tATGAACTATTTCAGATATATACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                     <  1:158851/141‑3 (MQ=255)
                                                                                          aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                    <  2:685402/141‑1 (MQ=17)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTTATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtag                                                   <  1:511527/141‑3 (MQ=12)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                   <  1:641023/141‑1 (MQ=17)
                                                                                            gtaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTCCTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                  >  2:299144/3‑141 (MQ=11)
                                                                                            gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCATTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTACGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                  <  2:194102/141‑1 (MQ=11)
                                                                                             aaCTATTTCAGATATTNCCAATTTAACGAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                 <  1:630962/141‑1 (MQ=255)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                 >  1:252477/1‑141 (MQ=17)
                                                                                              aCTATTTCAGAGATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                <  2:633571/141‑1 (MQ=12)
                                                                                               cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCg                                               >  1:2164/1‑141 (MQ=12)
                                                                                                tATTTCTGATATAAACAATTTAACAAGGATGTTATCACGCTAGCTGTTG‑‑GATACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                              <  1:286652/141‑1 (MQ=11)
                                                                                                 aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTACACGGTcaccggtt                                             >  2:474548/1‑134 (MQ=11)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                            >  1:745384/1‑141 (MQ=255)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                           >  2:383313/1‑141 (MQ=17)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                           <  1:228107/141‑1 (MQ=255)
                                                                                                      ggagataGACAATTTAACAAGGATGTTATTCCTGTTGCCGTTG‑‑GCTATTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                        <  2:133799/137‑1 (MQ=11)
                                                                                                        ctataAACAATTCAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTAATCGTGCTATATATGAAAAATGAAAGGAACGTGGTGTAACGATTCATCATTCAACGCACTACCGTTCGGGTCa                                      >  2:149809/2‑141 (MQ=11)
                                                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTCGGTTCAAg                                    >  1:389113/1‑141 (MQ=255)
                                                                                                          ataAACAATTGAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                    <  1:7777/141‑1 (MQ=255)
                                                                                                            aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                  <  1:648118/141‑1 (MQ=17)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTAAAGAat                                 >  1:295543/1‑141 (MQ=12)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                 <  1:236200/141‑1 (MQ=255)
                                                                                                               cAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                               <  2:363762/141‑1 (MQ=255)
                                                                                                               cAATTACACCAGGATGTTATCACTGTAGCCGTTGCCGATACTGCTGAGTGTGTGCTGTGCTATCGTCATATATCCTAAATAGTTAGAGAGCGTGGTGTAAGCGTTCATCGCTCAACGATCGCCCGTTGGGTTCAAGGattt                               <  2:161026/141‑3 (MQ=2)
                                                                                                                aaTTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                              <  1:482696/141‑1 (MQ=255)
                                                                                                                 aTTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                             <  1:406755/141‑1 (MQ=255)
                                                                                                                 aTTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                             >  2:523920/1‑141 (MQ=255)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                            <  1:719090/141‑1 (MQ=17)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                            >  1:654330/1‑141 (MQ=255)
                                                                                                                  attAAAAAGGATGTTATCACTGTAGCCGTTG‑‑GATACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATACTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                            <  1:360065/140‑1 (MQ=11)
                                                                                                                   ttAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAATCGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                           >  2:395037/1‑141 (MQ=255)
                                                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                          <  1:170279/141‑1 (MQ=255)
                                                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                          >  1:273178/1‑141 (MQ=255)
                                                                                                                     aaCAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                         >  1:571726/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCGCTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATACAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:426118/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATCTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:676443/1‑141 (MQ=17)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:205073/1‑141 (MQ=255)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:585142/1‑141 (MQ=255)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCATTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:54545/1‑141 (MQ=11)
                                                                                                                       cAAGGATGTTATCACTGTAGCGGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                       <  2:110566/141‑1 (MQ=255)
                                                                                                                        aaGGATGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGACTATGCCCCAGtt                      >  2:227438/1‑141 (MQ=12)
                                                                                                                        aaGGATGTTATCACTGTAGCCGGTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                      <  1:752930/141‑1 (MQ=255)
                                                                                                                         aGGTTGGTATGACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATGGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                     <  2:432062/141‑1 (MQ=11)
                                                                                                                          ggATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                    >  1:474922/1‑141 (MQ=255)
                                                                                                                            aTGTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                  <  1:623349/141‑1 (MQ=18)
                                                                                                                              gTTATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                <  2:248641/141‑1 (MQ=25)
                                                                                                                               ttATCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAACGTAAGGTTCATGAATATGCCCCAATTTTATATc               >  2:731247/1‑141 (MQ=11)
                                                                                                                               ttATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc               >  2:745248/1‑141 (MQ=255)
                                                                                                                                tagcACTGTAGCCGTTG‑‑GCTACTATCTAAGAAATGCAGTGGGCCATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACGGTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa              <  1:141198/138‑1 (MQ=11)
                                                                                                                                tATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa              <  1:692221/141‑1 (MQ=35)
                                                                                                                                 atgaCTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATCTTAAGGGGAAGTGGTGTAAACGTTGATCATTCAACGGCGTAACGGTGGGTGCAAGAATAGGCCACAAttctctttaca             >  2:679347/4‑132 (MQ=11)
                                                                                                                                 aTCACTGTAGCTGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGGGTAAACGTTCATAATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  2:618194/1‑141 (MQ=255)
                                                                                                                                 aTCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  2:330414/1‑141 (MQ=255)
                                                                                                                                  tCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa            >  2:467878/1‑141 (MQ=255)
                                                                                                                                  tCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCGGGCGTGATATATCTGAAAGATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa            >  1:726177/1‑141 (MQ=11)
                                                                                                                                   cACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTCTATCAAAt           >  1:322047/1‑141 (MQ=255)
                                                                                                                                     cTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTTTATATCAAAttt         >  2:23217/1‑141 (MQ=25)
                                                                                                                                     cTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt         <  1:379810/141‑1 (MQ=255)
                                                                                                                                        tAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  1:187050/141‑1 (MQ=255)
                                                                                                                                        tAGCCGTTA‑‑GCTACTATCTAAGGTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  2:671440/141‑1 (MQ=11)
                                                                                                                                          gCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag    >  1:44613/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTG‑‑GCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: