Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 27.3% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT27.3% 167.3 / 60.6 99intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/33);  new base T (13/14);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

TTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36441‑36718
                                                                                                                                            |                                                                                                                                         
ttCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGGTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAc                                                                                                                                           >  1:724255/1‑141 (MQ=17)
       aaTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                                                                                                                                    <  1:650688/141‑1 (MQ=35)
        atgtgtCAGATATATCACGATAACTACATGAATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                                                                                                                                   <  2:485960/136‑1 (MQ=11)
              cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                                                                                                                             >  1:93647/1‑141 (MQ=35)
               aGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACttt                                                                                                                            <  1:578819/141‑1 (MQ=35)
                aatatatCACGATAACTCAATGCATATCTTAGATGGTAGCCAGCAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                                                                                                           <  1:471335/140‑1 (MQ=255)
                    tatCCCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       <  1:207680/141‑1 (MQ=25)
                    tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCaaa                                                                                                                       >  2:402712/1‑140 (MQ=21)
                    tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       >  1:484238/1‑141 (MQ=35)
                    tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       >  1:745558/1‑141 (MQ=35)
                    taaaagcgtctcTCAATGGATACCATCGTTAATAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       <  1:361769/130‑1 (MQ=11)
                       cACGATAACTCAATTTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGa                                                                                                                    <  1:336527/141‑1 (MQ=25)
                       cACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGATGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGa                                                                                                                    <  2:492918/141‑1 (MQ=25)
                        aCGCTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa                                                                                                                   >  1:552389/1‑141 (MQ=25)
                           aTAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg                                                                                                                <  2:445236/141‑1 (MQ=9)
                            tagctgaaTGCATTTCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               <  2:113210/135‑1 (MQ=11)
                            tagcTCAATGCATATTTTAGATAGTAGCCAACGGCTAAAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCCTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               <  2:197692/138‑1 (MQ=11)
                            tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               >  2:8100/1‑141 (MQ=37)
                            caaCGCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTACAACAGAACCGt                                                                                                               >  2:702215/2‑141 (MQ=255)
                             agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCCGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAAATTTGCAACAGAACCGta                                                                                                              >  2:696215/3‑141 (MQ=11)
                             agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCATGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCATTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                                                                                                              >  2:160272/3‑141 (MQ=11)
                             aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                                                                                                              >  1:221879/1‑141 (MQ=37)
                              gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTATTTATACCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTCTGCAACAGAACCGtat                                                                                                             <  2:654891/140‑1 (MQ=11)
                              aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  1:635992/1‑141 (MQ=39)
                              aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             <  1:17649/141‑1 (MQ=38)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                            <  1:686373/141‑1 (MQ=21)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTGTTGGTAAAAATATATTATAAATTAAACTTTGCCACAGAACCGtatt                                                                                                            >  1:630094/1‑141 (MQ=11)
                                tCAATGTATATCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTTTTAAATTTTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                           <  2:184189/141‑1 (MQ=255)
                                 cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                          >  1:491910/1‑139 (MQ=255)
                                  aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta                                                                                                         >  1:756034/1‑138 (MQ=255)
                                   atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                                                                                                        >  1:18648/5‑137 (MQ=255)
                                   aTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                                                                                                        <  2:549090/141‑5 (MQ=255)
                                    tgcatatTTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  2:33424/138‑6 (MQ=255)
                                     gcatatCTTAGATAGTAGCCCACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGGCTCCTTTTTGTTAATATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                      <  2:409006/139‑7 (MQ=11)
                                      catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                     >  1:135794/2‑129 (MQ=11)
                                      catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                     >  1:704966/2‑129 (MQ=11)
                                      catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTGTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                     <  1:511280/140‑8 (MQ=11)
                                       atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATCGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCACATTTGCAACAGAACCGtattttattagt                                                                                                    >  2:225588/1‑133 (MQ=11)
                                       atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagt                                                                                                    >  1:612166/1‑133 (MQ=255)
                                        tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTATTATAAATTCAACTTTGCAACAGAAACTtatttttttagaa                                                                                                   >  2:568520/1‑132 (MQ=11)
                                        tatCTTAGATAGTAGCCAACGGCTAAAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAACATTATACTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                                   <  1:149622/141‑15 (MQ=11)
                                         atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                                  >  1:307173/1‑141 (MQ=255)
                                          tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTCTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATagag                                                                                                 <  1:510075/141‑1 (MQ=255)
                                           cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCTTATTATGGAATAgaga                                                                                                <  1:455341/141‑1 (MQ=255)
                                           cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAgaga                                                                                                <  1:12830/141‑1 (MQ=255)
                                           cTTAGATAGTAGCCAACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAgaga                                                                                                <  2:568563/141‑1 (MQ=255)
                                            ttAGATTGTAGCCAACGGCTACAGTGATAAGATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                               <  2:148026/141‑14 (MQ=11)
                                            ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                               <  2:508713/141‑14 (MQ=11)
                                            ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                               <  2:236328/141‑14 (MQ=11)
                                            ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATAATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                               >  1:401756/1‑128 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACTGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              >  1:400897/1‑127 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              <  1:51245/141‑15 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGCTGATAACTGAACTAGTTCAAGCAGACTACTCGTTTATGTTAAACGTATATGATAAATTCAACTTTGCAACAGAACCGtattttatcactacactc                                                                                              <  2:281129/141‑15 (MQ=2)
                                                    gagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                       <  1:626945/140‑1 (MQ=255)
                                                      gccgttggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAATATACTATAAATTCAACTTTGCAACAGAACAGGATTATGGAATAGAGATGTTGAGAACa                                                                                     >  1:379308/7‑141 (MQ=14)
                                                       ccAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTAATTTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAt                                                                                    >  2:382882/1‑141 (MQ=255)
                                                        cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                   >  1:341412/1‑141 (MQ=255)
                                                         aaCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAttt                                                                                  <  1:46287/141‑1 (MQ=255)
                                                            ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                               >  1:573712/1‑141 (MQ=255)
                                                               tACTGTGATAACATCCTTGTTAAATTGTTTATATCTGAATTAGTGCATACAGAAGAATCCTTTTTGTTAAAATTATACTATAAATTCAACATTGCAACGAAACCTAATTATGGAATAGAGATGTTGGTACCCTTTATACAg                                                                            >  2:302707/1‑141 (MQ=17)
                                                                aCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTTCTCTAAATACAACTTTCCAACATAACCGTATTATGTATTAGAGCTGTTGGTAACATTCATAccgc                                                                           >  2:265868/1‑138 (MQ=17)
                                                                aCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAgg                                                                           <  2:676196/141‑1 (MQ=255)
                                                                aCAGTGATAACAGCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAGTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTTACATTGATACatg                                                                           >  1:529183/1‑139 (MQ=255)
                                                                aCAGTGATAACACTCTTGTTAAATTGTCTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAgg                                                                           <  1:445787/141‑1 (MQ=255)
                                                                  aGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGAt                                                                         >  2:412696/1‑141 (MQ=255)
                                                                   gTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATc                                                                        <  2:484238/141‑1 (MQ=255)
                                                                     gATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTCGTTAAAATTATACTATAAATTCAACTGTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCAt                                                                      <  1:380129/141‑1 (MQ=255)
                                                                      aTAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCAtt                                                                     <  1:35258/141‑1 (MQ=255)
                                                                        aaCATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTTTTTTTACTATAAATTCAACTTCACAACAGAACCGTACTATGGAATAGAGATGATGGTAACATTTTTACGCGATCTTtat                                                                   >  2:325700/1‑141 (MQ=17)
                                                                        aaCATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATtat                                                                   >  1:632482/1‑141 (MQ=255)
                                                                          cATCCTTGTTAAATTGTTTATATTTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCCACAGTACCGGATGATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATAc                                                                 <  2:350903/141‑1 (MQ=21)
                                                                             ccTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGGTCATTATACTTa                                                              >  1:426622/1‑141 (MQ=255)
                                                                             ccTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTa                                                              >  1:294866/1‑141 (MQ=255)
                                                                                    aaaTTGTTTTTATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                       >  1:563004/1‑141 (MQ=255)
                                                                                    aaaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                       >  2:582409/1‑141 (MQ=255)
                                                                                        tGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTGTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTc                                                   <  1:474778/141‑1 (MQ=255)
                                                                                        tGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATCCTTAAGTTTAATTTc                                                   >  1:68552/1‑141 (MQ=255)
                                                                                          tttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTATTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATATTTAAGTTTAATTTGGt                                                 >  2:199292/1‑141 (MQ=25)
                                                                                           ttATATCTGAAATAGTTCATACAGAAGACCCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtt                                                <  1:209641/141‑1 (MQ=255)
                                                                                             atatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttat                                              <  2:135965/141‑1 (MQ=255)
                                                                                                        aGTTCATACAGAAGACTCCTTTCTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                   <  2:483533/141‑1 (MQ=255)
                                                                                                         gTTCATACAGAAGACTCCTTTTTGTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGATTAGAGATGTTGGTAGCCTTTATACCGGCTCATTATACTTATGTTTAATTTCGttattccttcctttct                                  >  2:304994/1‑130 (MQ=21)
                                                                                                           tCATACAGAAGACTCCTTTTTGTTTATTTTTTTCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGCTAACATTTATACAGGATCATTATACTTAAGTTTAATATCGTTATTACAGAACcacaca                                >  1:300366/1‑141 (MQ=38)
                                                                                                            cATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGaacaaccaat                               >  2:178604/1‑137 (MQ=255)
                                                                                                              tACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTCCAAAACCACCCATTc                             >  2:217158/1‑141 (MQ=255)
                                                                                                              aaCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTc                             <  1:236000/140‑1 (MQ=255)
                                                                                                               aCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTCTGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                            >  2:191739/1‑141 (MQ=255)
                                                                                                                   aagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAAcc                        >  2:238426/1‑141 (MQ=255)
                                                                                                                   aagaATCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTGAATTTCGTTATTACAGAACCACACATTCCAAcc                        >  1:522435/1‑141 (MQ=255)
                                                                                                                    ataCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCa                       <  1:703715/139‑1 (MQ=255)
                                                                                                                    agaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACATTATCATTATACTTAAGTTTAATTTCGTTATTACAGAACAACACATTCCAACCa                       <  1:402712/141‑1 (MQ=255)
                                                                                                                     gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                      <  1:71592/141‑1 (MQ=255)
                                                                                                                          cTTTTTGTTAAAATTATACTATAAATCCACGGCGGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACAACTTCCAACCAGAtgag                 >  2:507925/1‑141 (MQ=255)
                                                                                                                           tttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                <  2:267058/141‑1 (MQ=255)
                                                                                                                             ttggTTAAAATTATAATTTAAGTGTAACTTTGCAACCGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACACGATCATTATACTAAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaaa              <  2:647411/138‑1 (MQ=255)
                                                                                                                                   aaaaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGt        >  2:366451/1‑141 (MQ=255)
                                                                                                                                    aaaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTc       >  1:548875/1‑141 (MQ=255)
                                                                                                                                     aaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCt      <  1:412696/141‑1 (MQ=255)
                                                                                                                                         ataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAttt  >  1:743664/1‑141 (MQ=255)
                                                                                                                                         ataCTATAAATTAAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTACTTTCGTTATTCAAGAACCACACATTACACGCAGAAGCAAAAGGATGGCGAttt  >  2:148789/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36441‑36718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: