Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,571,957 A→G 4.1% intergenic (‑109/+117) panE1 ← / ← USA300HOU_2433 2‑dehydropantoate 2‑reductase/MFS family major facilitator transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,571,9570AG 4.1% 328.5 / 2.8 112intergenic (‑109/+117)panE1/USA300HOU_24332‑dehydropantoate 2‑reductase/MFS family major facilitator transporter
Reads supporting (aligned to +/- strand):  ref base A (56/50);  new base G (3/2);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 117 total aligned reads displayed.

AATGCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGTTTGATTTTTA  >  CP000730/2571817‑2572095
                                                                                                                                            |                                                                                                                                          
aaTGCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCta                                                                                                                                            >  1:379881/1‑141 (MQ=255)
 aTGCACCCGATCCATCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCtat                                                                                                                                           <  1:783910/141‑1 (MQ=255)
 aTGCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCCTCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCtat                                                                                                                                           >  2:404636/1‑141 (MQ=255)
 aTGCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCtat                                                                                                                                           <  1:61213/141‑1 (MQ=255)
   gCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATaa                                                                                                                                         <  2:1191632/141‑1 (MQ=255)
      cccGATCCAGCAATAGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATGTACACACAGCTTTTATCGTCTATAAAAg                                                                                                                                      >  1:661208/1‑141 (MQ=255)
         gATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAac                                                                                                                                   <  1:916039/141‑1 (MQ=255)
         gATCCAGCAATTGCAATTTTCACACTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAac                                                                                                                                   <  1:287363/141‑1 (MQ=255)
          aTCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCCATTTTAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAACGAacc                                                                                                                                  >  2:277500/1‑140 (MQ=255)
          aTCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGATTAATATGCACATAGAATGTATGTTCTGTAAAAGaaaa                                                                                                                                  >  1:321360/1‑139 (MQ=255)
           tCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAacac                                                                                                                                 <  2:1087238/141‑1 (MQ=255)
             cAGCAATTGCAATTTTCATCCTCTACACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAacacac                                                                                                                               >  1:285673/1‑141 (MQ=255)
             cAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATATAAGAacacac                                                                                                                               >  1:362273/1‑141 (MQ=255)
             cAGCAATTGCAATTTTCATCCTATAAACACCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAGTATGCACACAGCTTTTATCCTCGATAAAAGAacacac                                                                                                                               >  1:698147/1‑141 (MQ=255)
              aGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAATAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATACAAGCACACAcc                                                                                                                              >  2:599072/1‑141 (MQ=255)
              aGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATCAAAGAacaaccc                                                                                                                              >  1:446587/1‑137 (MQ=255)
              aGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACAcc                                                                                                                              >  2:478857/1‑141 (MQ=255)
                caattgcaattTTCATCCTATAAACACCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTa                                                                                                                            <  2:281897/141‑1 (MQ=255)
                   ttgtaattTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACa                                                                                                                         <  2:724010/141‑1 (MQ=255)
                    tgcaattTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACAt                                                                                                                        <  1:248281/141‑1 (MQ=255)
                     tctattTTTATCCTATAACCCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACAtt                                                                                                                       <  2:463821/138‑1 (MQ=255)
                      caattTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTa                                                                                                                      <  1:1136581/141‑1 (MQ=255)
                       aattTTCATCCTATAAACCGCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTaa                                                                                                                     >  2:906077/1‑141 (MQ=255)
                          tttCATCCTATAAACCCCTCTTCATCGTCGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACg                                                                                                                  <  1:922145/141‑1 (MQ=255)
                              aTCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCaa                                                                                                              >  1:913822/1‑141 (MQ=255)
                              aTCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTGTCGCAGATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCaa                                                                                                              >  1:1065840/1‑141 (MQ=255)
                                ccTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATa                                                                                                            >  1:505319/1‑141 (MQ=255)
                                ccTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATa                                                                                                            >  1:889311/1‑141 (MQ=255)
                                ccTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAAAACACCTAACATTAAACGTCAATa                                                                                                            >  1:838538/1‑141 (MQ=255)
                                   ataAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATCCga                                                                                                         >  2:224998/1‑140 (MQ=255)
                                     aaaCCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCaa                                                                                                       <  1:858181/141‑1 (MQ=255)
                                       aCCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCAGACAGCTTTTATCGTCTGTAAAAGAACACACCTAACATTAAACGTCAATACGCAACa                                                                                                     >  2:695930/1‑141 (MQ=255)
                                       aCCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACa                                                                                                     >  2:104888/1‑141 (MQ=255)
                                          ccTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTa                                                                                                  <  2:60863/141‑1 (MQ=255)
                                           ctctTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTaa                                                                                                 >  1:1019952/1‑141 (MQ=255)
                                                cATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAgtgtg                                                                                            >  1:1058831/1‑141 (MQ=255)
                                                  tCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTACCATTAAACGCCAATACGCAACATTAAGTGTCTa                                                                                          >  2:457242/1‑141 (MQ=255)
                                                  tCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATCCGCAACATTAAGTGTGTa                                                                                          >  2:607260/1‑141 (MQ=255)
                                                  tCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATAATTCGCTTAATATGCACACGGCTTTTATCGTCTATAAAAGAGCACACCTACCATTAAACGTCAATACGCAACATTAAGTGTGTa                                                                                          >  2:527682/1‑141 (MQ=255)
                                                       gttgttCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATa                                                                                     >  1:553791/1‑141 (MQ=255)
                                                       gttgttCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATa                                                                                     <  2:797401/141‑1 (MQ=255)
                                                        ttgttCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATaa                                                                                    <  2:462815/141‑1 (MQ=255)
                                                          gttCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAac                                                                                  >  1:235928/1‑141 (MQ=255)
                                                          gttCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATCCGCTTAATATGAAAACAGCATTTCACGTCTATAAAAGCACACACCTCACATTAACCGTCAATACGCCACATTAAGTGAGTATGCTacac                                                                                  >  2:128410/1‑141 (MQ=255)
                                                               ttttAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAacttact                                                                             <  1:832724/141‑1 (MQ=255)
                                                               ttttAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAacttact                                                                             >  2:800601/1‑141 (MQ=255)
                                                                   aaaaaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTca                                                                         <  1:650939/141‑1 (MQ=255)
                                                                    aataTACTATTTTCCCATATTAGCGTCAATTTGAAAATCATTCGCTTAATCTGCACACAGCCTTTATCGTCTATAAAAGAACACACCTAACATTATACGTCAATACGCAACATTAAGTGTGTCTGATATACTTACTTTccc                                                                        <  2:923947/138‑3 (MQ=255)
                                                                    aaaaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTCTAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATACACTTACTTTcac                                                                        >  2:7148/1‑141 (MQ=255)
                                                                    aaaaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTcac                                                                        <  1:682624/141‑1 (MQ=255)
                                                                    aaaaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTcaa                                                                        >  1:1094278/1‑140 (MQ=255)
                                                                     aaaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTcaca                                                                       >  1:647231/1‑141 (MQ=255)
                                                                      aaTACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACATAACATTAAACGTCAATACCAAACATTAAGAGTGTAAGATAAACTTACTTTCACaa                                                                      >  2:911725/1‑141 (MQ=255)
                                                                        tactacTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACaaaa                                                                    >  2:1066483/1‑141 (MQ=255)
                                                                         cctactTTCGCAATGTAGCGTCAATTTGAAAAGCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACAGTAAACGTCAATACGCAACATTAAGTGTGTATAATAAACTTACTTTCACAAAAt                                                                   <  2:679025/140‑1 (MQ=255)
                                                                            actTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTc                                                                <  2:87946/141‑1 (MQ=255)
                                                                            actTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTc                                                                <  2:838538/141‑1 (MQ=255)
                                                                             ctTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTcc                                                               <  1:733785/141‑1 (MQ=255)
                                                                              ttacGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGAAATTAGGACTTACTTTCACAAAATTTCCt                                                              <  2:848892/138‑1 (MQ=255)
                                                                               ttCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCtt                                                             >  1:369589/1‑141 (MQ=255)
                                                                               ttCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCtt                                                             <  1:877695/141‑1 (MQ=255)
                                                                                 cGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTTAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTa                                                           <  1:1041595/141‑1 (MQ=255)
                                                                                 cGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTa                                                           <  1:404636/141‑1 (MQ=255)
                                                                                  gCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTaa                                                          <  2:487549/141‑1 (MQ=255)
                                                                                    atatTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTaaaa                                                        >  2:338250/1‑141 (MQ=255)
                                                                                    atatTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTaaaa                                                        >  2:84506/1‑141 (MQ=255)
                                                                                    atatTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTaaaa                                                        <  2:1179993/141‑1 (MQ=255)
                                                                                     tatTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTGTAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAAt                                                       <  1:695930/141‑1 (MQ=255)
                                                                                           cGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTAc                                                 >  2:532287/1‑141 (MQ=255)
                                                                                                aTTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACataat                                            >  2:81606/1‑141 (MQ=255)
                                                                                                       aaTCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGc                                     <  1:791057/141‑1 (MQ=255)
                                                                                                        aTCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCt                                    <  1:478857/141‑1 (MQ=255)
                                                                                                         tCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtt                                   <  2:194532/141‑1 (MQ=255)
                                                                                                          cATTCGCTTAATATGCAGACAGCTTTTGTCGTCTATAAAAGAACACACCTAACCTCAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCttt                                  >  1:625351/1‑141 (MQ=255)
                                                                                                             tCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtttcat                               <  1:589475/141‑1 (MQ=255)
                                                                                                              cGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtttcatt                              >  1:301404/1‑141 (MQ=255)
                                                                                                               gCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCACTACATAATAACTTGCtttcattt                             >  2:725174/1‑141 (MQ=255)
                                                                                                                cTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtttcatttc                            >  1:59160/1‑141 (MQ=255)
                                                                                                                cTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtttcatttc                            >  2:57614/1‑141 (MQ=255)
                                                                                                                 ttAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCtttcatttca                           <  2:1081802/141‑1 (MQ=255)
                                                                                                                     tttGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGttt                       <  2:572538/139‑1 (MQ=255)
                                                                                                                        tcacacaGCTTTTATCTACTATAAATGAACACACCTATCATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACCTAATAACTTGCTTTCATTTCAGttttcc                    <  2:1188974/140‑3 (MQ=255)
                                                                                                                        gCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTTCTTTCATTTCAGTTTTAc                    <  2:423757/141‑1 (MQ=255)
                                                                                                                        gCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTAc                    <  2:1175648/141‑1 (MQ=255)
                                                                                                                        gCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACATACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTAc                    <  2:886298/141‑1 (MQ=255)
                                                                                                                         cacacaGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACa                   <  1:906077/141‑1 (MQ=255)
                                                                                                                         cacacaGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACa                   <  1:687507/141‑1 (MQ=255)
                                                                                                                            ccaGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAAt                <  2:286570/140‑1 (MQ=255)
                                                                                                                             caGCTTTTATCGTCTATAAAAGCACCGCGTTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATACCTTGCTTTAATTTCAGTTTTACAAAtt               >  2:548174/1‑141 (MQ=255)
                                                                                                                               gCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCg             <  1:104888/141‑1 (MQ=255)
                                                                                                                               gCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCg             >  1:1190715/1‑141 (MQ=255)
                                                                                                                               gCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCg             >  1:687426/1‑141 (MQ=255)
                                                                                                                                 ttttATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGtt           >  2:689506/1‑141 (MQ=255)
                                                                                                                                 ttttATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGtt           >  2:109550/1‑141 (MQ=255)
                                                                                                                                   ttATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGTTTg         >  2:475571/1‑141 (MQ=255)
                                                                                                                                   ttATCGTCTATAAAAGAACACACCTAACATTAAACGACAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTACTTTAACATCCACACCTACAGCGAATCTTTCATTTCGCTTTTACAACTTCGTTTg         >  2:539869/1‑141 (MQ=255)
                                                                                                                                    tATCGACTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTGTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGTTTGa        <  2:532381/141‑1 (MQ=255)
                                                                                                                                     aTCGTCTATAAAAGAACACACCTAACATTAACCGGCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGTTTGAt       <  1:316635/141‑1 (MQ=255)
                                                                                                                                         tCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTCCAAATTCGTTTGAttttt   >  2:721633/1‑141 (MQ=255)
                                                                                                                                          cTATAAAAGAACACACCTAACATTAAACGTCAATACGCCACATTAAGTGTGTATGATAAACTTACTTTCACAGAATTTCCTTTAAAATCCCTACGTAATAACTTGCTGTCGTTTGGGTTTTTCAAATTCGTTTGgttgttt  >  1:437445/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AATGCACCCGATCCAGCAATTGCAATTTTCATCCTATAAACCCCTCTTCATCGTTGTTGTTCATTTTAAAAATACTACTTTCGCATATTAGCGTCAATTTGAAAATCATTCGCTTAATATGCACACAGCTTTTATCGTCTATAAAAGAACACACCTAACATTAAACGTCAATACGCAACATTAAGTGTGTATGATAAACTTACTTTCACAAAATTTCCTTTAAAATCCCTACATAATAACTTGCTTTCATTTCAGTTTTACAAATTCGTTTGATTTTTA  >  CP000730/2571817‑2572095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: