Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,749,331 C→A 35.6% G40C (GGT→TGT)  secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,749,3310CA35.6% 121.6 / 94.7 108G40C (GGT→TGT) secDFRND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (38/31);  new base A (17/21);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 112 total aligned reads displayed.

TTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCATTATTTACGTA  >  CP000730/1749191‑1749471
                                                                                                                                            |                                                                                                                                            
ttcAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCAcc                                                                                                                                              <  1:921139/141‑1 (MQ=255)
   aaCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTg                                                                                                                                           <  2:154077/141‑1 (MQ=255)
    aCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGc                                                                                                                                          <  1:603420/141‑1 (MQ=255)
     cTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCa                                                                                                                                         >  1:177044/1‑141 (MQ=255)
      ttGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTACTGTTTGCGCTGTTTATTGTAAGGCTTTTTTATCTCTTTTGTCGCCTTTTTTTAAAGGATCGACTTTGTAAAGCACCTCAAATCCACCTTGCaa                                                                                                                                        <  2:209920/141‑1 (MQ=255)
       tGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCaaa                                                                                                                                       >  1:441326/1‑141 (MQ=255)
       tGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCaaa                                                                                                                                       <  1:252474/141‑1 (MQ=255)
          aTTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCa                                                                                                                                    <  2:872687/141‑1 (MQ=255)
           ttttAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCaa                                                                                                                                   >  2:747517/1‑141 (MQ=255)
               aGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTcc                                                                                                                               >  1:1133139/1‑141 (MQ=255)
               aGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTcc                                                                                                                               >  1:708533/1‑141 (MQ=255)
                 gTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTa                                                                                                                             >  2:583833/1‑141 (MQ=255)
                  ttCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTaa                                                                                                                            <  2:141790/141‑1 (MQ=255)
                     cGAATCACCTAATACGTTTACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAAtt                                                                                                                         <  2:19038/141‑1 (MQ=255)
                     cGAAACTCCTAAGCCGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTGTTTATCTATTTTGTCGCCTTTATTTAAAGTATCGACTTGATAAAGCACCTTAAATTCACCCTGCAAATCAAGTCCTAAAtt                                                                                                                         <  2:850663/141‑1 (MQ=255)
                       aaaCACCTAAAACGTTTACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGa                                                                                                                       <  2:609217/141‑1 (MQ=255)
                          cacCTAAAACGTTAACACGATTTTCTAATGTTTTCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAGGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGCCAt                                                                                                                    >  2:789881/1‑141 (MQ=255)
                           acCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACAtt                                                                                                                   >  1:733913/1‑141 (MQ=255)
                            ccTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAACGCACCTCAAATCCACCTTGCAAATCTAGTCCTAAATTGACATTc                                                                                                                  >  2:706448/1‑141 (MQ=255)
                              tAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATCTCTAATCAAGTCCTAAATTGACATTCtt                                                                                                                <  2:848544/141‑1 (MQ=255)
                              tAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGGCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCtt                                                                                                                <  2:358546/141‑1 (MQ=255)
                                aaaCGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTta                                                                                                              >  2:290242/1‑141 (MQ=255)
                                    gTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGAAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTACTAAATTGACATTCTTTATAac                                                                                                          >  1:829630/1‑141 (MQ=255)
                                     ttcacacCATTTTCTGATGGTTTCGCGGTTGATTGTAAGGCGTCTCTCTCTCTTGTGTCGCCTTTATTTAAAGGATCTCCTTGATAAAGCCTCTCAAATCCCCCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAaca                                                                                                         <  2:69630/138‑1 (MQ=255)
                                     ctAACCCTATTTTCTTATGTTTTGGTTGTTGATTGTAATGCTTTTTTATCTATTTTTTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAAGCAAGTCCTAAATTGACATTCTTTATAaca                                                                                                         <  1:466621/140‑1 (MQ=255)
                                      tAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAacac                                                                                                        >  1:483201/1‑141 (MQ=255)
                                       aaCACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACt                                                                                                       <  1:66628/141‑1 (MQ=255)
                                        acacGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACtt                                                                                                      <  1:311281/141‑1 (MQ=255)
                                            gATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTtat                                                                                                  <  2:153793/141‑1 (MQ=255)
                                                ttCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGAGAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAACTTGACATTCTTTATACCACTTTTATaact                                                                                              >  2:582358/1‑139 (MQ=255)
                                                ttCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTCGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGt                                                                                              >  2:134536/1‑141 (MQ=255)
                                                 tCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATGGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGtt                                                                                             >  2:600943/1‑141 (MQ=255)
                                                    aaTGTTTGCGCTGTTGATTGTAAGGCTTTTTTAGCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCa                                                                                          <  1:814548/141‑1 (MQ=255)
                                                     aTGTTTGCGCTTTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                                                                         <  2:409967/141‑1 (MQ=255)
                                                     aTGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATACTTTATAAACCTTATATAATTTGCAg                                                                                         >  1:455287/1‑141 (MQ=255)
                                                     aTGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                                                                         <  1:1127257/141‑1 (MQ=255)
                                                     aTGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                                                                         >  2:23256/1‑141 (MQ=255)
                                                     aTGTTTGCGCTGTTGATTGTAAGGCTTTGTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                                                                         >  1:773703/1‑141 (MQ=255)
                                                      tGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGc                                                                                        <  1:233903/141‑1 (MQ=255)
                                                            cgcTTTTTATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCTAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTcc                                                                                  <  2:883431/141‑1 (MQ=255)
                                                                 ttGATTGTAAGGCTTTTTTAACTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAACACACCGCAAATCCACCTTCCAAACCACGTCCTAACTTAACATTCTTTATGACACTTTTATATGTTGCAGCCATTCAGCCaa                                                                             >  2:603867/1‑141 (MQ=255)
                                                                   tagtGTTAGGCTTTTTTGTCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAaac                                                                           <  2:888587/138‑1 (MQ=255)
                                                                    attggtatgCTTTGTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAaaca                                                                          <  2:857959/133‑1 (MQ=255)
                                                                    aTTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAaaca                                                                          <  2:602586/141‑1 (MQ=255)
                                                                      tGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAaacaac                                                                        <  2:777381/141‑1 (MQ=255)
                                                                        tAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAAcaacaa                                                                      >  2:973002/1‑141 (MQ=255)
                                                                        tAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAAcaacaa                                                                      >  1:335733/1‑141 (MQ=255)
                                                                           ggCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATAc                                                                   <  2:177044/141‑1 (MQ=255)
                                                                           ggCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATAc                                                                   <  2:343572/141‑1 (MQ=255)
                                                                             cTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGa                                                                 >  1:853579/1‑141 (MQ=255)
                                                                              ttttttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGAc                                                                <  2:708533/141‑1 (MQ=255)
                                                                              ttttttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGAc                                                                >  1:95751/1‑141 (MQ=255)
                                                                                ttttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCATCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCTTTCCGGCCAACAACAATACGACTa                                                              >  1:320288/1‑141 (MQ=255)
                                                                                ttttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTa                                                              >  1:534860/1‑141 (MQ=255)
                                                                                 tttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCCGCCATTCCGGCAAACAACAATACGACTaa                                                             >  1:209159/1‑141 (MQ=255)
                                                                                   tATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTaaaa                                                           >  1:329175/1‑141 (MQ=255)
                                                                                     tcgaTTTTGGTGCCTTTACTTAAAGGATCGACTCGATAAAGCACCTCAAATCCACTTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTCTATAAGTTGCAGCCATTCCGGCATACAACAATACGACTAAAAGc                                                         <  2:654802/138‑1 (MQ=255)
                                                                                              tCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCa                                                <  1:6028/141‑1 (MQ=255)
                                                                                              tCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCa                                                <  2:335733/141‑1 (MQ=255)
                                                                                               cGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCaa                                               >  2:877369/1‑141 (MQ=255)
                                                                                                   tttatttaAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGTCAAACAACAATACGACTAAAAGCAAGAACGCAattat                                           <  1:525126/141‑1 (MQ=255)
                                                                                                      atttaAAGGATCGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTct                                        <  2:1042346/141‑1 (MQ=255)
                                                                                                       tttaAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTcta                                       >  2:960585/1‑141 (MQ=255)
                                                                                                        ttaAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTctac                                      <  2:893086/141‑1 (MQ=255)
                                                                                                         tagaGGCTCGAGTGGACAGAGCACCGCACAGCCACATGGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTCTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAGTGATTctact                                     <  2:499257/138‑1 (MQ=255)
                                                                                                         taAAGGATCGACTGGATAAAGCACCTCAAATCCACATTGCTAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTctacg                                     <  2:608195/141‑2 (MQ=255)
                                                                                                          aaaGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTctacta                                    >  1:186936/1‑141 (MQ=255)
                                                                                                           aaGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCtactac                                   >  1:1132713/1‑141 (MQ=255)
                                                                                                           aaGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATACTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCtactac                                   >  1:164688/1‑141 (MQ=255)
                                                                                                            aGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTactact                                  >  1:660470/1‑141 (MQ=255)
                                                                                                                 cGACTTGATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTttct                             <  1:290242/141‑1 (MQ=255)
                                                                                                                  gACTTTATAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTttctt                            >  2:1038320/1‑141 (MQ=255)
                                                                                                                  gACTTGATAAAGCACCTCAAGTCCACATTGCAAATCAAGTCCTAGATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTttctt                            >  2:652639/1‑141 (MQ=255)
                                                                                                                    cTTTATAAAGCACCTCAAAACCACCTTGCAAATCAAGTTCAAAATTGACATTCTTTATAACACTTGTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACGTTTATTca                          <  2:315494/141‑1 (MQ=255)
                                                                                                                       gATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAGTTATTCTGCTACTTTTCTCAACAt                       >  1:995758/1‑141 (MQ=255)
                                                                                                                       gATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACAt                       >  2:867606/1‑141 (MQ=255)
                                                                                                                       gATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACAt                       >  1:1193813/1‑141 (MQ=255)
                                                                                                                       gATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTAACAt                       >  1:995875/1‑141 (MQ=255)
                                                                                                                        aTAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATg                      >  2:67083/1‑141 (MQ=255)
                                                                                                                         tAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATCCGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGa                     >  1:454469/1‑141 (MQ=255)
                                                                                                                         tAAAGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGa                     >  1:631843/1‑141 (MQ=255)
                                                                                                                          aaaGCACCTCAAATGCACCTTGCAAATCTAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGaa                    >  1:781439/1‑141 (MQ=255)
                                                                                                                          aaaGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGaa                    >  1:1103589/1‑141 (MQ=255)
                                                                                                                            aGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCtactac                                   <  1:665655/124‑1 (MQ=255)
                                                                                                                            aGCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCtactac                                   >  2:665655/1‑124 (MQ=255)
                                                                                                                             gCACCTCAAATCCACCTTGCGAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTCTTCTACTACTTTTCTTCACCTGAgcac                 >  2:905805/1‑141 (MQ=255)
                                                                                                                             gCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAacac                 <  2:401550/141‑1 (MQ=255)
                                                                                                                             gCACCTCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAacac                 >  1:441674/1‑141 (MQ=255)
                                                                                                                             gCACCTCAAAGCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAacac                 <  1:23256/141‑1 (MQ=255)
                                                                                                                                 cTCAAATCCACCTTGCAAATCAAGTACTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCa             <  1:1172909/141‑1 (MQ=255)
                                                                                                                                  tCAAATCCACATTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCat            <  1:538075/141‑1 (MQ=255)
                                                                                                                                   cAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCatt           >  2:833163/1‑141 (MQ=255)
                                                                                                                                   cAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCatt           >  2:833100/1‑141 (MQ=255)
                                                                                                                                    aaaTCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCattt          >  2:1049017/1‑140 (MQ=255)
                                                                                                                                    aaaTCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCAAATGAACACTTCattt          >  2:152484/1‑140 (MQ=255)
                                                                                                                                      aTCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCattatt        >  2:626406/1‑141 (MQ=255)
                                                                                                                                      aTCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCattatt        <  1:233452/141‑1 (MQ=255)
                                                                                                                                           ccTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACACTACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCATTATTTACgt   >  2:966129/1‑141 (MQ=255)
                                                                                                                                           ccTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCATTATTTACgt   >  1:745954/1‑141 (MQ=255)
                                                                                                                                            cTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCATTATTTACgta  >  2:307072/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGAACGCAATTATTCTACTACTTTTCTTCACATGAACACCTCATTATTTACGTA  >  CP000730/1749191‑1749471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: