Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 829,785 A→G 3.4% K89E (AAA→GAA)  USA300HOU_0778 → possible superfamily II DNA/RNA helicase ComFC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730829,7850AG 3.4% 351.5 / 2.5 117K89E (AAA→GAA) USA300HOU_0778possible superfamily II DNA/RNA helicase ComFC
Reads supporting (aligned to +/- strand):  ref base A (74/38);  new base G (2/2);  total (77/40)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 122 total aligned reads displayed.

ATATTAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATCTAGAACA  >  CP000730/829645‑829925
                                                                                                                                            |                                                                                                                                            
atatTAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTGAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTAGATTGTGAATTTCAATATGACGGTTTAATGa                                                                                                                                              >  2:749602/1‑141 (MQ=255)
 tatTAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGaa                                                                                                                                             <  1:240373/141‑1 (MQ=255)
   ttAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGCAag                                                                                                                                           >  2:1075486/1‑141 (MQ=255)
   ttAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATAACGGTTTAATGAAag                                                                                                                                           >  2:941342/1‑141 (MQ=255)
     agaGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAagag                                                                                                                                         <  1:967555/139‑1 (MQ=255)
      aaGCAAGGCGCTGTACAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAgaga                                                                                                                                        >  2:811485/1‑141 (MQ=255)
       aGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTAGCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAAGGAAAGAgat                                                                                                                                       <  2:45912/141‑1 (MQ=255)
        gCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAgatg                                                                                                                                      <  2:352430/141‑1 (MQ=255)
         cAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGGAAGAgatga                                                                                                                                     >  1:1173152/1‑141 (MQ=255)
         cAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGGAAGAgatga                                                                                                                                     >  1:914821/1‑141 (MQ=255)
          tttgtgATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAgatgat                                                                                                                                    <  2:1197331/136‑1 (MQ=255)
          aaGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATAAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAgatgat                                                                                                                                    <  1:336829/141‑1 (MQ=255)
           aGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGGTGATa                                                                                                                                   >  1:278557/1‑141 (MQ=255)
           aGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATa                                                                                                                                   >  2:25568/1‑141 (MQ=255)
           aGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATa                                                                                                                                   >  1:73297/1‑141 (MQ=255)
            ggCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATAc                                                                                                                                  >  1:582360/1‑141 (MQ=255)
             gCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTGTTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATCGAACAATTATATTGTCAATCTCAATATGACGGTTTAATGAAAGAGATGAAACa                                                                                                                                 >  2:655275/1‑141 (MQ=255)
              cGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTCATGAAAGAGATGATACAt                                                                                                                                >  2:1058440/1‑141 (MQ=255)
               gaagTTCAAGGTGCTTAAAACAATTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATc                                                                                                                               <  2:310778/138‑1 (MQ=255)
               gATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATc                                                                                                                               <  2:588605/141‑1 (MQ=255)
                 tGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATTTGACGGTTTAACGAAAGAGATGATACATCAg                                                                                                                             >  2:281677/1‑141 (MQ=255)
                   ttCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGta                                                                                                                           <  1:427846/141‑1 (MQ=255)
                       aagTGCTCAAAACAACTAAATCAAAATGCAGTGTTTTTTTTAGACGATAAGTTTCTATCGGTACAAATTAATATAGTATAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATaaa                                                                                                                       <  2:191292/139‑1 (MQ=255)
                         gTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAAtt                                                                                                                     >  1:289428/1‑141 (MQ=255)
                         gTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAAtt                                                                                                                     >  1:650088/1‑141 (MQ=255)
                                 aaCACTTAAATCAAGATTAAGCGTATTGTTTAGACTGCAAGTTTCTACCGTCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAg                                                                                                             <  1:105497/141‑1 (MQ=255)
                                 aaCACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAg                                                                                                             >  2:121067/1‑141 (MQ=255)
                                 aaCACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAg                                                                                                             <  1:352779/141‑1 (MQ=255)
                                  acacTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGa                                                                                                            >  1:712486/1‑141 (MQ=255)
                                    acTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACt                                                                                                          <  1:694437/141‑1 (MQ=255)
                                    acTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACt                                                                                                          <  1:1089884/141‑1 (MQ=255)
                                     cTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTACTGGAACAATTATATTGTCAAGTTCAATATGACTGTTTAATGAAAGAGATGGTACATCAGTATAAATTTTTGAAACACta                                                                                                         >  1:360863/1‑141 (MQ=255)
                                     cTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACta                                                                                                         >  1:148536/1‑141 (MQ=255)
                                     cTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACta                                                                                                         >  1:957912/1‑141 (MQ=255)
                                       tAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACtatt                                                                                                       >  1:906204/1‑141 (MQ=255)
                                        aaaTCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACtatta                                                                                                      >  1:681138/1‑141 (MQ=255)
                                              aGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTAtg                                                                                                <  2:1178467/141‑1 (MQ=255)
                                               gATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTAtgt                                                                                               >  2:200948/1‑141 (MQ=255)
                                               gATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTAtgt                                                                                               >  1:148036/1‑141 (MQ=255)
                                                    aGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAatt                                                                                          >  1:992904/1‑141 (MQ=255)
                                                      cGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATAGATCAGTATAAATTTTTGAAAGACTAATATTTATGTGAatttt                                                                                        >  2:242333/1‑139 (MQ=255)
                                                      cGTATTGTTTAGACTGCAAGGTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAattat                                                                                        <  1:918500/141‑1 (MQ=255)
                                                            gTTTAGACTGCAAGTTTCTATCGACACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAGAGACTATTATTTATGTGAATTATTAGcac                                                                                  >  1:602953/1‑141 (MQ=255)
                                                                  aCTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttga                                                                            <  1:140003/141‑1 (MQ=255)
                                                                   atGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCGCTGTCAATATGACGGTTTAATGAAAGTGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttgat                                                                           <  1:224977/140‑1 (MQ=255)
                                                                     gCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttgattg                                                                         >  2:1058569/1‑141 (MQ=255)
                                                                      cAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATGTTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttgattga                                                                        >  2:597102/1‑141 (MQ=255)
                                                                      cAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttgattga                                                                        >  2:359020/1‑141 (MQ=255)
                                                                      cAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATttgattga                                                                        >  2:346231/1‑141 (MQ=255)
                                                                        aGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATGTTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGaaa                                                                      >  2:597272/1‑141 (MQ=255)
                                                                        aGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGaaa                                                                      >  1:42253/1‑141 (MQ=255)
                                                                        aGTTTCTATCGGCACACTTGAATTTAATGGAACAATTATATTGTCAATTTCAATATGACTGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCAGATTTGATTGaaa                                                                      >  2:668354/1‑141 (MQ=255)
                                                                         gTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGaaaa                                                                     >  2:279902/1‑140 (MQ=255)
                                                                         gTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGaaaa                                                                     >  1:373535/1‑140 (MQ=255)
                                                                         gTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAAt                                                                     >  1:131000/1‑141 (MQ=255)
                                                                         gTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATCTGATTGAAAt                                                                     >  1:163254/1‑141 (MQ=255)
                                                                             cTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACca                                                                 >  2:499872/1‑141 (MQ=255)
                                                                               aTCGGCACACTTTAATTTAATGGAACACTTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACcaaa                                                               >  1:1077347/1‑139 (MQ=255)
                                                                                tCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTAAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACaa                                                              >  1:933047/1‑141 (MQ=255)
                                                                                   gCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACa                                                           >  1:1150341/1‑141 (MQ=255)
                                                                                   gCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAAAGATGATACATTAGTATGAATTTTTGAAAGACTATTATTTTTGTGAATTATTGGCACATTTGATTAAAATACaacaaaca                                                           >  2:443227/1‑141 (MQ=255)
                                                                                    cacaCTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACAt                                                          <  1:340753/141‑1 (MQ=255)
                                                                                        cTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTa                                                      >  1:1085724/1‑141 (MQ=255)
                                                                                         tttaatttaaTGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACAACAAACATCTTAt                                                     >  1:582864/1‑141 (MQ=255)
                                                                                           taattttatGGGACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGa                                                   <  2:801214/141‑1 (MQ=255)
                                                                                           taatttaatGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGa                                                   >  1:762803/1‑141 (MQ=255)
                                                                                           taatttaatGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTGTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAGCATCTTATGa                                                   >  2:896920/1‑141 (MQ=255)
                                                                                           taatttaatGGAACAATGATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACAGTTGATTGAAATACCACAAATATCTTaggg                                                   >  1:762939/1‑138 (MQ=255)
                                                                                              tttaatGGGACAATGGTAGTTTCAATTTCAGTATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGCAATATTTGATTGAAATACCACAAACATCTTATGACta                                                >  2:1146837/1‑141 (MQ=255)
                                                                                                 aatGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTatat                                             <  1:200948/141‑1 (MQ=255)
                                                                                                  atGGAAGAATTATATTGTCAATGTCAATATGACGGTTTAATGAAAGAGATTATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATAtt                                            <  2:8168/141‑1 (MQ=255)
                                                                                                  atGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACGTCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTGTAtt                                            >  1:228578/1‑141 (MQ=255)
                                                                                                  atGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTCTTGGCACATTTGATTGAAATACCACAAACATCTTCTGACTATCtt                                            >  1:239362/1‑141 (MQ=255)
                                                                                                  atGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATAtt                                            >  1:693821/1‑141 (MQ=255)
                                                                                                  atGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACCATTATTTATGTGACTTATTGGCACACTTGATTCAAATACCACAAACATCTTATGACTATAtt                                            <  2:777373/141‑1 (MQ=255)
                                                                                                      aaaaaTTTTATTGTCAATTTGAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGc                                        <  2:619956/138‑1 (MQ=255)
                                                                                                       aCAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGCTTGAAATACCACAATCATCTTATGACTATATTGTGcc                                       >  2:1097040/1‑141 (MQ=255)
                                                                                                       aCAATTATATTGTCAATTTCAATAGGACGGTTTAATGAACCGAAAGTCTGGGCACGATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGcc                                       <  1:1171799/141‑1 (MQ=255)
                                                                                                              tatTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCct                                >  1:487811/1‑141 (MQ=255)
                                                                                                              tatTGTCAATTTCAATATGACGGTTGAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCGCATTTGATTGAAATACCACAAACATCTTATGCTTATATTATGCCAATTCct                                >  1:504866/1‑141 (MQ=255)
                                                                                                               atTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCctt                               <  2:1042180/141‑1 (MQ=255)
                                                                                                                  gTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCcttctt                            <  2:10246/141‑1 (MQ=255)
                                                                                                                       tttCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCg                       >  1:361217/1‑141 (MQ=255)
                                                                                                                       tttCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCg                       >  1:469637/1‑141 (MQ=255)
                                                                                                                       tctCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAACATTTGAAACCATATACTAAATGTGAATTAGTTGCACATGTGAGTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCg                       >  1:236521/3‑141 (MQ=255)
                                                                                                                            atatGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGcaca                  >  2:72906/1‑141 (MQ=255)
                                                                                                                            atatGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGcaca                  <  1:200107/141‑1 (MQ=255)
                                                                                                                            atatGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGCcaca                  >  2:647773/1‑141 (MQ=255)
                                                                                                                                 aCGGTTTAATGGAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGAtt             <  2:914821/141‑1 (MQ=255)
                                                                                                                                 aCGGTTTAATGGAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGAtt             <  2:1173152/141‑1 (MQ=255)
                                                                                                                                 aCGGTTTAATGAAAGCGATGATACATCAGTATAAATTTTGGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCTCATGAtt             <  1:749602/141‑1 (MQ=255)
                                                                                                                                   tgTTTAATGAAAGAGATGATACATCAGTATAAATTTTNTGAAGTTTATTTTATATGTGAATAATTTGTACATTTGTTTGCAATACTACAATCATCTGTTCACTAAATTGTGCCAAATCCGACTTCGTCGGCACATGaagta           <  2:278557/140‑5 (MQ=255)
                                                                                                                                     tttAATGAACGAGATGATACATCAGTATAAATTTTTTAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACAAATAACATCTTCTCTCCGTTATAAGCCAATTCCTTCTTCGCCGGCACGTGATTTATc         <  1:876030/141‑1 (MQ=255)
                                                                                                                                     tttAATGAAAGAGATGATACATCAGTGTAAATTGTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATc         <  2:796597/141‑1 (MQ=255)
                                                                                                                                     tttAATGAAAGAGATGATACATCAGTATTAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATc         >  1:760548/1‑141 (MQ=255)
                                                                                                                                     tttAATGAAAGAGATGATACATCAATATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATc         >  1:760600/1‑141 (MQ=255)
                                                                                                                                      ttAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGACACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTATTCGCGGGCACGTGATTTctct        >  2:151319/1‑141 (MQ=255)
                                                                                                                                          tGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATCTAGaa    >  2:546278/1‑141 (MQ=255)
                                                                                                                                          tGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATCTAGaa    <  2:957912/141‑1 (MQ=255)
                                                                                                                                            aaaGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATCTAGAACa  <  2:289428/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATATTAAAGCAAGGCGATGTTCAAGGTGCTTAAAACACTTAAATCAAGATGAAGCGTATTGTTTAGACTGCAAGTTTCTATCGGCACACTTTAATTTAATGGAACAATTATATTGTCAATTTCAATATGACGGTTTAATGAAAGAGATGATACATCAGTATAAATTTTTGAAAGACTATTATTTATGTGAATTATTGGCACATTTGATTGAAATACCACAAACATCTTATGACTATATTGTGCCAATTCCTTCTTCGCCGGCACATGATTTATCTAGAACA  >  CP000730/829645‑829925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: