Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 828,298 T→A 8.2% intergenic (+213/‑151) USA300HOU_0776 → / → USA300HOU_0777 hypothetical protein/possible superfamily II DNA/RNA helicase ComFA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730828,2980TA 8.2% 321.8 / 3.0 117intergenic (+213/‑151)USA300HOU_0776/USA300HOU_0777hypothetical protein/possible superfamily II DNA/RNA helicase ComFA
Reads supporting (aligned to +/- strand):  ref base T (57/46);  new base A (2/9);  total (61/56)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

ATCATTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAATTGTATATC  >  CP000730/828158‑828438
                                                                                                                                            |                                                                                                                                            
atcatTTGAAAGCCAAAATGCTAAGGATATGAGAAATGCCTTATATGACTGGTCTCTCTCTTAAGCTGACATGCGCTTCGATGCAGGATGCATTTCGCGGAATTAATTCATCCTCCTACTTCACTTACTAATGGAACAAtt                                                                                                                                              <  2:71886/141‑1 (MQ=255)
atcatTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAAtt                                                                                                                                              <  2:1136911/141‑1 (MQ=255)
  catTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTACTGAAAAAATTaa                                                                                                                                            >  1:58334/1‑141 (MQ=255)
  catTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTaa                                                                                                                                            <  1:92172/141‑1 (MQ=255)
   atTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTaaa                                                                                                                                           >  2:768286/1‑141 (MQ=255)
       taaaGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAaag                                                                                                                                       <  1:674254/140‑1 (MQ=255)
       gAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACAGTACGGGAAATTAATTCATCCTCATACTTGACTTTCTAACAAAAAAATCaaaaaca                                                                                                                                       >  2:181609/1‑139 (MQ=255)
         aaGCCAAACTGTTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTCCTAATGCAAAAATTAAAAaagaa                                                                                                                                     <  2:530392/141‑1 (MQ=255)
            ccAAAATGCTAAAGAAATAAGAAAATCATGATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTa                                                                                                                                  <  1:156956/141‑1 (MQ=255)
             cAAAATGCTAAAGATATAAGAAACGGTTGACAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTaa                                                                                                                                 >  1:880479/1‑141 (MQ=255)
             cAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACCTTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAACAATTAAAAAAGAAGTaa                                                                                                                                 >  1:465372/1‑141 (MQ=255)
             cAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTaa                                                                                                                                 >  2:291448/1‑141 (MQ=255)
              aaaaTGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAAc                                                                                                                                <  2:926840/141‑1 (MQ=255)
                 aTGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGGAGTAACAgg                                                                                                                             >  1:998159/1‑141 (MQ=255)
                 aTGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAgg                                                                                                                             >  1:533225/1‑141 (MQ=255)
                   tgtAAAGAAAAAAAAAAAAAAATATAATATTAGGCTCTTTTATACGTTGAAATGAGGCTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGgtg                                                                                                                           <  2:683366/139‑1 (MQ=255)
                   tcTAAATATATAAGAAAATCATTATAATATTATTCTTTTTTTTACGTTTAAATGATTTTTTAATCATTAAACATTACTGGAAATTAATTCATCCTCATACTTCACTTACTAATAAAAAAATTAAAAAAGAAGTAACatttg                                                                                                                           <  2:1084915/140‑5 (MQ=255)
                      aaaGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGtca                                                                                                                        >  2:783472/1‑141 (MQ=255)
                       aaGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTGGAAATGAAGTTCTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGtcat                                                                                                                       <  2:696289/141‑1 (MQ=255)
                            ataAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGGTGTCATcaaac                                                                                                                  <  2:889435/141‑1 (MQ=255)
                             taAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATcaaaca                                                                                                                 <  2:508665/141‑1 (MQ=255)
                             taAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATcaaaca                                                                                                                 <  1:828261/141‑1 (MQ=255)
                             aaaGAAATTCATTATAATATTAGGCTCTGCTTTATGTTGAAATGCGGTTTAAAGCATTACTCATTACGTGACATTAATTCATCCGCATACTTCACTTACTAATGAGAATAGTACAAAAGAAGTAACAGGTGTCatcaatca                                                                                                                 <  2:109610/140‑1 (MQ=255)
                             aaaGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGGTGTCATcaaaca                                                                                                                 <  2:179129/140‑1 (MQ=255)
                             aaaGAAAATCATTAAAATATTAGGATCTTTTTAACGTTGAAATGAGCTTTTAAGCATTAAACATTACGAGAAATTAATTCATCCTCATACTTCACTTACTAAAGAAAAAAAAAAAAAAGAAGTAACAGGTGTCATcaaaca                                                                                                                 <  2:1112284/140‑1 (MQ=255)
                              aaGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATcaaacaa                                                                                                                >  1:176638/1‑141 (MQ=255)
                              aaGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAAAAGGTGTGATcaaacaa                                                                                                                >  1:1169983/1‑141 (MQ=255)
                              aaGAAAAGCATTATAATATTAGGCCCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATCCTTCTAACACAGATGAAAAAAAGAAAAAAGAAGTAACAGGTGTCATcaaacaa                                                                                                                >  2:540097/1‑141 (MQ=255)
                               aGAAAATCATTATAATATTAGGCTCTTTTTTTTTTTTTTTTGAGGTGTTAAGCATTAAACATTACGGGAAATTAATTCATCATCATACTTCACTGACTTATGAAAAAATTAAAAAAGAAGTAACAGGTGTAATcaaacaaa                                                                                                               >  2:627027/1‑141 (MQ=255)
                               aGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATcaaacaaa                                                                                                               >  2:1191553/1‑141 (MQ=255)
                               aGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATcaaacaaa                                                                                                               >  1:26820/1‑141 (MQ=255)
                                  aaaTCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAAtt                                                                                                            <  1:673251/141‑1 (MQ=255)
                                    aTCATTATAATATTAGGCTCTTTTTTTTTTTTTTTTTAAGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATCAAAAAATTAAAAAAAAAGTAACAGGTGTCATCAACCAAATTTCa                                                                                                          >  2:798609/1‑141 (MQ=255)
                                     tCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaa                                                                                                         <  2:396097/141‑1 (MQ=255)
                                      taTTATAATATTAGGCTTTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATGCTTCACTTACTAATAAAAAAAATAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaaa                                                                                                        <  2:444072/140‑1 (MQ=255)
                                      cATTTTAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTATAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATGAAACAAAATTCaaa                                                                                                        >  1:855911/1‑141 (MQ=255)
                                      cATTATAATATTAGTCTCTATTTTACGTTGAAATGAGGTATTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaaa                                                                                                        <  2:1179400/141‑1 (MQ=255)
                                      cATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaaa                                                                                                        <  2:423083/141‑1 (MQ=255)
                                      cATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaaa                                                                                                        <  1:65341/141‑1 (MQ=255)
                                      cATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCaaa                                                                                                        >  1:416423/1‑141 (MQ=255)
                                       aTTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAAc                                                                                                       <  1:1160960/141‑1 (MQ=255)
                                            aataTTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACtatta                                                                                                  >  1:744834/1‑141 (MQ=255)
                                              tatTAGGCTTTTTTTTACGTTAAAATGAGGTATTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTAATAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTattatt                                                                                                <  2:1068614/141‑1 (MQ=255)
                                              tatTAGGCTCTTTTTTACGTGGAAATGAGGTGTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTattatt                                                                                                <  2:369158/141‑1 (MQ=255)
                                                   ggCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGtt                                                                                           <  1:150924/141‑1 (MQ=255)
                                                       ctTTATTACGTTGAAATGAGGTTTTAAGCAATAAACATTACGGGACATGACGTCATCATCATACGGTCCTAACTAATGAAAAAATAAAACAGGAAATACAAGGTGTCATCAAACACACTGCAAACTAGTAACgtgtgcgcg                                                                                       >  2:727258/1‑136 (MQ=255)
                                                           tttACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTTATTGTGa                                                                                   >  2:123122/1‑141 (MQ=255)
                                                           tttACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTAATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTAAAACACTTATTATGTTCAATGTGa                                                                                   >  2:1109142/1‑141 (MQ=255)
                                                             tACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAACAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTCTTAATCTGTTCAATGTGaaa                                                                                 >  2:842127/1‑141 (MQ=255)
                                                             tACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGaaa                                                                                 >  1:590098/1‑141 (MQ=255)
                                                             tACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACGTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGaaa                                                                                 >  1:721348/1‑141 (MQ=255)
                                                              aCGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAg                                                                                <  1:1196467/141‑1 (MQ=255)
                                                                gTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTa                                                                              <  1:324556/141‑1 (MQ=255)
                                                                 ttGAAATGAGGTTTTAAGCATTAACCATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTGTAATGTGAAAGTaa                                                                             >  2:663042/1‑140 (MQ=255)
                                                                 ttGAAATGAGGTTTTAAGCATTAAACATTACGAGAAATTAATTAATCCTCATACTTCAATTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAGAAGTCAAACTATTATTGTGTTCAATGTGAAAGTAc                                                                             <  2:939937/141‑1 (MQ=255)
                                                                    aattGAGGTTTTAAGCATTACACATTACGGGAAATGAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACaaa                                                                          <  2:896422/138‑1 (MQ=255)
                                                                       tGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATcc                                                                       >  1:342016/1‑141 (MQ=255)
                                                                          ggTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCaca                                                                    >  2:175702/1‑139 (MQ=255)
                                                                           gctttAAGCATTAAACATTACGGGAAATTAATTTATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCGAAAg                                                                   <  1:813003/139‑1 (MQ=255)
                                                                           gTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTTTTGTTTTCCATGTGAAAGTACAAATCCAAAg                                                                   <  2:496384/141‑1 (MQ=255)
                                                                           gTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAATAAAAGGTGTCATCAAACAAAATTAAAAATATAATTTTGTTCAATGTGAAAGTACAAAACCaaaa                                                                   >  2:509776/1‑140 (MQ=255)
                                                                            ttttAAGCATTAAACATTACGGGAAAATAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGc                                                                  >  1:390606/1‑141 (MQ=255)
                                                                                 aGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCAtttt                                                             >  2:1117145/1‑141 (MQ=255)
                                                                                   cATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTa                                                           <  1:383468/141‑1 (MQ=255)
                                                                                   aataaaccactACGGAAAATTAATTCATCCTCAGACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTa                                                           <  2:551590/131‑1 (MQ=255)
                                                                                    aTTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTAt                                                          >  2:509806/1‑141 (MQ=255)
                                                                                    aTTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAACATTTTTAt                                                          >  2:352473/1‑141 (MQ=255)
                                                                                    aTTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGGTCAATGTGAAAGTACAAATCCAAAGCATTTTTAt                                                          >  2:1117739/1‑141 (MQ=255)
                                                                                     ttAAACATTACGAGAAATTAATTAATCCTCATACTTCACTTACTAAAAAAAAAAAAAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATc                                                         <  2:212702/141‑1 (MQ=255)
                                                                                      tAAACATTACGGGAAATTTATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAACCATTTTTGTcg                                                        >  1:1134429/1‑140 (MQ=255)
                                                                                        aaCATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGt                                                      <  2:737796/141‑1 (MQ=255)
                                                                                        aaCATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGt                                                      <  2:287080/141‑1 (MQ=255)
                                                                                            ttACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGa                                                  >  1:189181/1‑141 (MQ=255)
                                                                                            ttACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTGAAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGa                                                  >  1:55223/1‑141 (MQ=255)
                                                                                             tACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGCTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGAt                                                 >  2:721431/1‑141 (MQ=255)
                                                                                                gggAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTAATATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTcc                                              <  1:777419/141‑1 (MQ=255)
                                                                                                  gAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCGTTATGGTTCCTc                                            <  2:1161929/141‑1 (MQ=255)
                                                                                                  gAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTc                                            <  1:852173/141‑1 (MQ=255)
                                                                                                      ttaattCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTa                                        <  1:435104/141‑1 (MQ=255)
                                                                                                         attCATCCTCATACTTCACTTACTAAGGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACAt                                     <  2:474673/141‑1 (MQ=255)
                                                                                                           tCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTAAAAATCAAAAGCATTTTTATCAGTATGATTCATAAGTACATTc                                   >  2:775450/1‑141 (MQ=255)
                                                                                                              tCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCaa                                >  1:1036316/1‑141 (MQ=255)
                                                                                                                    tACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAAt                          >  2:112220/1‑141 (MQ=255)
                                                                                                                    tACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAAt                          <  1:786464/141‑1 (MQ=255)
                                                                                                                      cTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCAAAGAAAAttt                        >  1:328677/1‑140 (MQ=255)
                                                                                                                      attCACTGACTAATGAAAAGATTAAAAAAGAAGTAACAGGTGTCATCGAACAAAATTCAACCTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTg                        <  2:495416/140‑1 (MQ=255)
                                                                                                                          aCTTACTAATGAAAAAATTCAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTCTTATTGTGTTCAATGTGAAAGCACAAATCCAAAACATTTTTATCAATGTGATTCCTCAGTCCATTCCAAGAAAATAGtaga                    >  2:666999/1‑139 (MQ=255)
                                                                                                                          aCTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAATAAAATTGtata                    >  2:313516/1‑141 (MQ=255)
                                                                                                                          aCTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGtata                    >  1:475191/1‑141 (MQ=255)
                                                                                                                            ttACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATAtt                  <  1:783472/141‑1 (MQ=255)
                                                                                                                             tACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTg                 <  2:304286/141‑1 (MQ=255)
                                                                                                                                tAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGAAg              >  2:932479/1‑141 (MQ=255)
                                                                                                                                tAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCAAAGAAAATTGTATATTGCAg              >  2:326400/1‑141 (MQ=255)
                                                                                                                                tAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATAAGTATGATTCCTCAGTAAATTCCAAGAAAATTGTATATTGCAg              >  2:10021/1‑141 (MQ=255)
                                                                                                                                   tGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAACATTTTTATCAGTATGAATCCTCAGTACATTCCAAGAGAATTGGATATAGCAGaaa           >  2:41096/1‑141 (MQ=255)
                                                                                                                                    aaaaaaaTGAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAACGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAAt          <  2:494548/140‑1 (MQ=255)
                                                                                                                                     aaaaaaTTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTTTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAAtt         >  1:775157/1‑141 (MQ=255)
                                                                                                                                     aaaaaaTTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATTAGTATGATTCCTCAGTAAATTCCAAGAAAATAGTATATTGCAGAAttt         >  2:234216/1‑141 (MQ=255)
                                                                                                                                        aaaTTAAAAAAGAAATAACAGGTTTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAATTGta      <  1:335218/141‑1 (MQ=255)
                                                                                                                                            tAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAATGGTATATc  >  1:670332/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATCATTTGAAAGCCAAAATGCTAAAGATATAAGAAAATCATTATAATATTAGGCTCTTTTTTACGTTGAAATGAGGTTTTAAGCATTAAACATTACGGGAAATTAATTCATCCTCATACTTCACTTACTAATGAAAAAATTAAAAAAGAAGTAACAGGTGTCATCAAACAAAATTCAAACTATTATTGTGTTCAATGTGAAAGTACAAATCCAAAGCATTTTTATCAGTATGATTCCTCAGTACATTCCAAGAAAATTGTATATTGCAGAAATTGTATATC  >  CP000730/828158‑828438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: