Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,672 C→A 29.3% V488F (GTT→TTT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,6720CA29.3% 153.6 / 79.2 113V488F (GTT→TTT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (43/36);  new base A (18/15);  total (62/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 117 total aligned reads displayed.

TTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTG  >  CP000730/39534‑39811
                                                                                                                                          |                                                                                                                                            
ttGTAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTAAGCATTATAAAATGGATAATCACTTGGTATATCTTCATCAacac                                                                                                                                            <  2:198396/141‑1 (MQ=255)
ttGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAaaac                                                                                                                                            >  2:277890/1‑141 (MQ=255)
 tGAAAGGATCTGTACTGGGTTACTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACAcc                                                                                                                                           <  1:1050867/141‑1 (MQ=255)
 tGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACAcc                                                                                                                                           <  1:1102079/141‑1 (MQ=255)
  gAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCt                                                                                                                                          <  2:562624/141‑1 (MQ=255)
    aaGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAg                                                                                                                                        >  1:517084/1‑141 (MQ=255)
      ggATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGtt                                                                                                                                      >  1:942657/1‑141 (MQ=255)
        aTCTGTACTGGGTTAATCAGTATTTCACCTTGCCCGTAACGTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCAGCAAAACCTAGtttt                                                                                                                                    >  1:856773/1‑141 (MQ=255)
         tCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTCACCTGAATCAGCTAATAATATTTGATTATCTAAATTTTTGTTTGGAATTTGAGCATTATAAAATGGATAAGTACTTGGTATATCTTCCCTAACATATAtttttt                                                                                                                                   >  1:189511/1‑141 (MQ=255)
           tGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTACTAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTc                                                                                                                                 <  1:815376/141‑1 (MQ=255)
           tGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTACTAATATTTCAACCTATAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAATACCTAGTTTTTTc                                                                                                                                 >  2:490728/1‑141 (MQ=255)
           tGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTc                                                                                                                                 >  1:846326/1‑141 (MQ=255)
            tgaCTGGGTTAATCATTATTTCTCCTTGTCCGTAACCTGAATCAGCTAATAATCTTTCATTATCTAAATTTTTGTTTGAAATTTTAGCATTATAAAATGTATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                                                <  2:22050/139‑1 (MQ=255)
            gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGACTCAGCTAATAATCTTTCATTATCTAAATTTTTGTTTGAAATTTAAGCATTATAACATGGATAATCACTTGGTATATCTTCACCACAACCAAGttttctat                                                                                                                                >  2:1014248/1‑137 (MQ=255)
            gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                                                <  1:708764/141‑1 (MQ=255)
            gTACTGGGTTAATCAGTATTTCACCCTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                                                <  1:103725/141‑1 (MQ=255)
             tACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAGATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCAt                                                                                                                               >  2:1004552/1‑141 (MQ=255)
              aCTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATg                                                                                                                              <  1:1047308/141‑1 (MQ=255)
                tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGcc                                                                                                                            >  1:826670/1‑141 (MQ=255)
                tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGcc                                                                                                                            <  1:859468/141‑1 (MQ=255)
                tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATCTTCGTTTGAAATTTGAGCATAATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTCGTTTTTTCATGcc                                                                                                                            >  2:373250/1‑141 (MQ=255)
                   gTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttt                                                                                                                         <  1:868062/141‑1 (MQ=255)
                   gTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTCCATTATCTTTAGTGTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCttt                                                                                                                         <  1:566511/141‑1 (MQ=255)
                     tAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttttt                                                                                                                       <  1:1050724/141‑1 (MQ=255)
                      aaTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTc                                                                                                                      >  1:337294/1‑141 (MQ=255)
                       aTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCTTTTTAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCa                                                                                                                     <  1:164806/141‑1 (MQ=255)
                          aGTATTTCACCTTCTCCGTAACCTTAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAAt                                                                                                                  <  2:348442/141‑1 (MQ=255)
                            tATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAAttt                                                                                                                >  2:986455/1‑141 (MQ=255)
                               ttCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATAACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCtt                                                                                                             >  2:158469/1‑141 (MQ=255)
                                tCACCTTGTCCGTATCCTGAATCAGCTAATGATATTTCATTATTTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTa                                                                                                            <  2:582026/141‑1 (MQ=255)
                                tCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAATTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTa                                                                                                            >  2:1148691/1‑141 (MQ=255)
                                 cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTAc                                                                                                           >  1:609479/1‑141 (MQ=255)
                                   ccTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTg                                                                                                         >  1:772170/1‑141 (MQ=255)
                                    cTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGc                                                                                                        <  1:229288/141‑1 (MQ=255)
                                     ttGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGcc                                                                                                       >  2:944375/1‑141 (MQ=255)
                                      tGTTCGTAACCTGAATTATCTAATAATTTTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCt                                                                                                      <  2:253482/141‑1 (MQ=255)
                                      tGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCt                                                                                                      <  1:696773/141‑1 (MQ=255)
                                       gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGACTTTTTCAAATTTCTTACTGCCTa                                                                                                     >  2:975715/1‑141 (MQ=255)
                                        tCCCTAACCTGAAACAGCTAATAATATTACATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTaa                                                                                                    <  2:328228/141‑1 (MQ=255)
                                             aaCCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGa                                                                                               <  2:903238/141‑1 (MQ=255)
                                             aaCCTGAATCAGATAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGa                                                                                               <  1:1078693/141‑1 (MQ=255)
                                              aCCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGCAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTATTTCAAATTTCTTACTGCCTAATTCGac                                                                                              >  1:675155/1‑140 (MQ=255)
                                               ccTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTAGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGt                                                                                             <  2:1152669/141‑1 (MQ=255)
                                                cTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGtt                                                                                            >  1:420790/1‑140 (MQ=255)
                                                cTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACAAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTg                                                                                            >  2:333709/1‑141 (MQ=255)
                                                 tGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGc                                                                                           <  2:1087442/141‑1 (MQ=255)
                                                   aaTCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTAACTGCCTAATTGGAGTGCtc                                                                                         >  1:217539/1‑140 (MQ=255)
                                                         cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAg                                                                                   >  1:208808/1‑141 (MQ=255)
                                                         cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAg                                                                                   >  1:308178/1‑141 (MQ=255)
                                                            ataataTTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTGTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTTGCaa                                                                                >  1:902595/1‑141 (MQ=255)
                                                              aataTTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCTTAATTCGAGTGCTACTCTAGCAAAg                                                                              >  2:953286/1‑141 (MQ=255)
                                                                  tttCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCaaaaaatt                                                                          <  2:1100153/141‑3 (MQ=255)
                                                                   ttaaTTATCTAAATTGTTGTTTGAAATTTGAGCATGATAAAATGGATAATACATTGGTATATCTTCACCAACACCTAGTTTTTTGATGCCTTTTTCCAATTTCTTACTGCCTAATTCGGGTGCTACTCTCGCCATGaaagt                                                                         >  2:265091/4‑139 (MQ=255)
                                                                    tCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTTTTTTTTTTTTCAAATTAATTACTCCCTAATTCAAGTGCATCTTTAGCAAAGAAGAtt                                                                        >  2:14622/1‑140 (MQ=255)
                                                                    tCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATg                                                                       <  2:517084/141‑1 (MQ=255)
                                                                      aTTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGtt                                                                     <  1:986687/141‑1 (MQ=255)
                                                                       ttATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTa                                                                    >  1:977295/1‑141 (MQ=255)
                                                                        tATCTAAATTTTTGTTTGAGATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGTTACTCTAGAAAAGAAAATGtttt                                                                   >  1:1004796/1‑139 (MQ=255)
                                                                            tAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtga                                                               <  2:460232/141‑1 (MQ=255)
                                                                            tAAATTTTTGTTTGAAATGTTAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtga                                                               <  2:212635/141‑1 (MQ=255)
                                                                                  tttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAACTGTTATCTGTTGAttt                                                         >  1:341881/1‑140 (MQ=255)
                                                                                   ttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGGGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCt                                                        >  1:1135196/1‑141 (MQ=255)
                                                                                    tgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTCTTCAAATTTCTTACTGCCTAATTCGAGTGCTTCTCTAGCAAAGAAAATGTTATCTGATGATTCTa                                                       <  2:638520/141‑1 (MQ=255)
                                                                                      tttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAtt                                                     >  1:74805/1‑141 (MQ=255)
                                                                                       ttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTTTTTTTTTTTTCTGCCTAATTCGAGTACTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAttc                                                    >  2:483943/1‑140 (MQ=255)
                                                                                        tgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGc                                                   <  2:35789/141‑1 (MQ=255)
                                                                                             tttGATCATTATAAAATTGATAATCACTTGCTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGtt                                              <  2:212397/141‑1 (MQ=255)
                                                                                               tGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTATCGCCAAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGtttt                                            >  1:1177610/1‑141 (MQ=255)
                                                                                                      tataCAATGGACAATCACCTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTTCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGa                                     <  2:16412/141‑1 (MQ=255)
                                                                                                          aaaTGGATAATCACTTGGTATATCTTCACCATCACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATAtt                                 <  2:442603/141‑1 (MQ=255)
                                                                                                            aTGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGGTATTAc                               >  1:127709/1‑141 (MQ=255)
                                                                                                             tGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTAcc                              >  2:116497/1‑141 (MQ=255)
                                                                                                               gATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAt                            <  1:595919/141‑1 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAtt                           >  2:468244/1‑141 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTCCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAtt                           >  1:313816/1‑141 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTTTTTTTTTTTACTGCCTAATTCGAGTGCTACTCTAGCAACGAATATGTTATCTGATGATTCTATTGCTTGTTTTATGTCGATATTACCAtt                           >  2:185464/1‑141 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGCTATTACCAtt                           >  1:1153490/1‑141 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAtt                           <  1:70951/141‑1 (MQ=255)
                                                                                                                aTAATCACTTGGTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAtt                           >  1:469063/1‑141 (MQ=255)
                                                                                                                 tAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAttt                          <  1:1148691/141‑1 (MQ=255)
                                                                                                                  aaTCTCTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTa                         <  2:204694/141‑1 (MQ=255)
                                                                                                                  aaTCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTa                         <  2:113328/141‑1 (MQ=255)
                                                                                                                   aTCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCATTCATAATAGATACAAAGGGTTCTATTCATTGCCTTAATTCGATATTACCATTTAc                        >  1:1171517/1‑141 (MQ=255)
                                                                                                                     cACTTGGTCTATCTTCACCAACACCTAGTTTGTTTATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCa                      <  2:625496/141‑1 (MQ=255)
                                                                                                                     cACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTTTTTTTTTTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGAATCTATTGCTTGTTTTAAGTAGATATTACCCTTTAccc                      >  2:733853/1‑140 (MQ=255)
                                                                                                                        ttGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACtt                   >  1:845933/1‑141 (MQ=255)
                                                                                                                        ttGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACtt                   >  1:659051/1‑141 (MQ=255)
                                                                                                                          ggTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCa                 >  1:738537/1‑141 (MQ=255)
                                                                                                                          ggTATATCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAAGTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTTAGTCGATATTACCATTTACCACTTCa                 >  1:724918/1‑141 (MQ=255)
                                                                                                                           gTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTTATTTTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTCCat                >  2:984291/1‑141 (MQ=255)
                                                                                                                           gTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCat                >  1:1078521/1‑141 (MQ=255)
                                                                                                                            tataTCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCata               <  1:116497/141‑1 (MQ=255)
                                                                                                                             atatCTTCACCAAAACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCatat              >  1:433144/1‑141 (MQ=255)
                                                                                                                                tCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCCAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCCCTCCATATCtt           >  2:39059/1‑141 (MQ=255)
                                                                                                                                tCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCCTTTACCACTTCATATCtt           >  1:783324/1‑141 (MQ=255)
                                                                                                                                    tcccAAAACCTAGTTTTTTCATGCCTTTTTCAAATCTCTTACTGCCTCATTCGAGTGTTAATCTAGCATCGAAAATGTTTTCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTaa       <  2:1026333/139‑1 (MQ=255)
                                                                                                                                       cAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGt    <  1:1063917/141‑1 (MQ=255)
                                                                                                                                        aaCACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAGGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTCACGtt   >  1:98317/1‑141 (MQ=255)
                                                                                                                                         acacCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTg  <  1:58367/141‑1 (MQ=255)
                                                                                                                                         acacCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTg  >  1:493796/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACTTCATATCTTGTAACGTTG  >  CP000730/39534‑39811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: