BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,797	G→A	56.7%	intergenic (‑880/‑288)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,797	0	G	A	56.7%	22.1 / 132.7	118	intergenic (‑880/‑288)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (29/22); new base A (26/41); total (55/63)
Fisher's exact test for biased strand distribution p-value = 6.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 119 total aligned reads displayed.

CTTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTA  >  CP000730/1929657‑1929934

ctTGTCATAATAAAATCGCCTACTTGCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTg                                                                                                                                                 <  2:562280/141‑1 (MQ=255)

          taaAATCGCCTACTTTCTTAAATTAACAATATCTATTCGCATAGAGTGTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCAGATAACTTATCCGAAGCAAGGGACTATGCGTGTATATTTGCCGATGatat                                                                                                                                       >  1:414113/1‑141 (MQ=25)

             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataat                                                                                                                                    >  1:394821/1‑141 (MQ=255)

             aaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataat                                                                                                                                    <  2:656158/141‑1 (MQ=255)

                cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                 <  1:147508/141‑1 (MQ=255)

                 gCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                <  2:832994/141‑1 (MQ=255)

                     tcTTTCTTAAATTAATAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGAGTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                            <  2:469758/140‑1 (MQ=255)

                     aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                            <  2:469942/141‑1 (MQ=255)

                         tCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGcc                                                                                                                        >  2:101620/1‑141 (MQ=255)

                            tAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAATGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                                     >  1:645611/1‑141 (MQ=255)

                                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctct                                                                                                                 >  2:781378/1‑141 (MQ=255)

                                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctct                                                                                                                 >  2:744222/1‑141 (MQ=255)

                                           tATTCTCATAGAATTTGTCCAATTAAGTGTAGACGACGCTATCTATCAGCTAAAATAAAAAAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTAGCCTATCTCTCAGGCGGCAAt                                                                                                      >  2:551843/1‑141 (MQ=38)

                                                tcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGa                                                                                                 >  2:733059/1‑141 (MQ=255)

                                                 cATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGAc                                                                                                >  2:441428/1‑141 (MQ=255)

                                                  aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACg                                                                                               >  1:22524/1‑141 (MQ=255)

                                                     gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATCTTTGCCGACGATATAATCATAGAGTCGCCTATCTCTCAGGCGGCACTTTCAACGccg                                                                                            >  2:550462/1‑139 (MQ=255)

                                                       aTTTGTGCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                          <  2:34544/141‑1 (MQ=255)

                                                       aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                          <  2:804382/141‑1 (MQ=255)

                                                           gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgagga                                                                                      >  2:829688/1‑141 (MQ=255)

                                                           gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgagga                                                                                      >  2:677448/1‑141 (MQ=255)

                                                                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                          >  1:747109/1‑141 (MQ=255)

                                                                       gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGGGTA‑TAA‑G‑G‑t                                                                      >  1:491417/1‑141 (MQ=255)

                                                                        tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTAACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑g                                                                    >  1:623881/1‑141 (MQ=255)

                                                                         aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATATCTCAGGCGTCAATTTAGTCGCAGTGAGGAGGTGTA‑TAA‑G‑G‑T‑Ga                                                                   <  1:516640/141‑1 (MQ=255)

                                                                             gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATgat                                                               >  2:836125/1‑139 (MQ=255)

                                                                                 cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTCCTTGTATATTTGCCGATGATATAATAGTAGCGTCGCCCATCTCTCCGGCGTCCATTTAGACGCAGAGAGGAGGTGTA‑TAG‑G‑G‑A‑GATGC‑TTAtt                                                          >  2:609540/1‑141 (MQ=255)

                                                                                    tCTATCAGCTAGAATCATATAACTTCACAGAAGAAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑G‑G‑T‑GCTGC‑TTCTTTTc                                                       >  2:839342/1‑141 (MQ=255)

                                                                                       aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGtt                                                    <  1:392400/141‑1 (MQ=255)

                                                                                        tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTc                                                   <  1:945882/141‑1 (MQ=255)

                                                                                          ggCTCGAAGCATATAACTTATCAAAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGGCGCCTATCTCTCTTGCGTCAATTTCGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTcac                                                 <  2:93428/140‑1 (MQ=255)

                                                                                               gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCAcatcat                                            >  1:136038/1‑141 (MQ=255)

                                                                                                             aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                              <  1:95442/141‑1 (MQ=255)

                                                                                                             aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                              >  1:500526/1‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGGACCAGTCATCAGTGGc                         >  1:470594/15‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  1:403493/15‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  1:18198/15‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  1:531183/15‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  2:233016/15‑141 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         <  1:385809/127‑1 (MQ=255)

                                                                                                                 atcggaagtagatatgCTTGGATGTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         <  1:588986/127‑1 (MQ=255)

                                                                                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                        >  1:800637/14‑141 (MQ=255)

                                                                                                                   tggaagtaggtatgCTTGTATATTCACAGATGGTATTATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCtg                       <  2:220925/129‑1 (MQ=255)

                                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                       >  1:809445/13‑141 (MQ=255)

                                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                       <  2:407656/129‑1 (MQ=255)

                                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                       >  2:552933/13‑141 (MQ=255)

                                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                       <  1:273132/129‑1 (MQ=255)

                                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                      <  1:698084/130‑1 (MQ=255)

                                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                      <  1:532799/130‑1 (MQ=255)

                                                                                                                     gaagtagatatgCTTGTATATTCACAGGTGGTACAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     <  1:229031/131‑1 (MQ=255)

                                                                                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     <  1:86919/131‑1 (MQ=255)

                                                                                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCtgtg                     <  1:615758/131‑1 (MQ=255)

                                                                                                                     gaagtagagtttcTTGGATATTCACAGTTGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     <  1:142484/129‑1 (MQ=255)

                                                                                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  2:111537/132‑1 (MQ=255)

                                                                                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  1:132841/132‑1 (MQ=255)

                                                                                                                      aagtagatatacTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  2:276159/130‑1 (MQ=255)

                                                                                                                      aaggagagatgCTTGTATATTCACAGATTGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  2:762431/132‑1 (MQ=255)

                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  1:441428/141‑1 (MQ=255)

                                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  2:406851/141‑1 (MQ=255)

                                                                                                                       aGTGAATATGCTTGTATATTCATAGATGGTATAATAATAGTGTCGTCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                   <  2:213878/141‑2 (MQ=255)

                                                                                                                        gtaggtatgCTTGTATATTCACAGATAGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑GCATTTTCGTTCACATCATAGCACTAGTCATCAGTGGCTGTGctg                  <  1:573061/134‑3 (MQ=255)

                                                                                                                        gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTA‑C‑T‑TGGCTAA‑TCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                  <  2:890004/131‑3 (MQ=255)

                                                                                                                        gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TGCGAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                  <  2:591319/134‑3 (MQ=255)

                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                  >  2:69433/8‑139 (MQ=255)

                                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGACTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAGTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTCGCcgttcgt                  >  2:278690/8‑137 (MQ=255)

                                                                                                                         tagatctgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                 <  2:23786/135‑4 (MQ=255)

                                                                                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                 <  2:743504/135‑4 (MQ=255)

                                                                                                                         tGACTGTGCTTGTATATTTGCGGATGATATAATAGGAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                  <  2:625450/141‑1 (MQ=255)

                                                                                                                          agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                >  1:745034/6‑137 (MQ=255)

                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg               <  1:650162/137‑6 (MQ=255)

                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg               >  1:430087/5‑136 (MQ=255)

                                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg               >  1:128562/5‑136 (MQ=255)

                                                                                                                           aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                <  2:529880/141‑1 (MQ=255)

                                                                                                                           aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                >  2:723604/1‑141 (MQ=255)

                                                                                                                           aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                >  1:185008/1‑141 (MQ=255)

                                                                                                                            atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc              <  1:510148/138‑7 (MQ=255)

                                                                                                                             tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc              <  2:198155/141‑1 (MQ=255)

                                                                                                                             tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc              <  1:755727/141‑1 (MQ=255)

                                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATCGAGTCGCCTACCTCTCAGGCGTCAATT‑‑GACGACGAGAGGAGTTGCA‑TTACT‑T‑T‑GCTAT‑GCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGctgttgcg             <  1:689404/139‑8 (MQ=255)

                                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg             <  2:716259/139‑8 (MQ=255)

                                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg             >  1:481777/3‑141 (MQ=255)

                                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg             >  1:568540/3‑141 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑T‑GCTAA‑TCATTTTGGTTCGCATCATAGAAACCACCATCAGTGGCTGTGctggtgcgt            >  2:704367/2‑133 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt            >  2:623953/2‑141 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt            >  2:936563/2‑141 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt            >  2:469908/2‑141 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt            >  1:829767/2‑141 (MQ=255)

                                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt            >  1:567940/2‑141 (MQ=255)

                                                                                                                                gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat          <  1:97220/141‑3 (MQ=255)

                                                                                                                                gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat          <  1:271449/141‑3 (MQ=255)

                                                                                                                                gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat          <  2:589049/141‑3 (MQ=255)

                                                                                                                                 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt         >  2:887091/1‑141 (MQ=255)

                                                                                                                                 cTTGGATATTCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt         <  1:803171/141‑1 (MQ=255)

                                                                                                                                    gTATATTCACAGATGGTATGATAATAGAGTAGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatc      >  1:501455/1‑138 (MQ=255)

                                                                                                                                    gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata      <  2:144288/141‑7 (MQ=255)

                                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac     <  1:758781/141‑8 (MQ=255)

                                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac     <  1:439643/141‑8 (MQ=255)

                                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac     <  2:436408/141‑8 (MQ=255)

                                                                                                                                     tataTTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac     <  1:391189/141‑8 (MQ=255)

                                                                                                                                         tttGCCGATGATATAATACTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTa  <  2:922020/141‑1 (MQ=255)

CTTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTA  >  CP000730/1929657‑1929934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`