Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,560 T→C 9.8% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5600TC 9.8% 288.2 / 20.2 116R210R (CGT→CGCUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (58/44);  new base C (2/9);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 2.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 116 total aligned reads displayed.

TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  CP000730/111422‑111699
                                                                                                                                          |                                                                                                                                           
tAAGTTTTTTGCGCAATATTGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                           <  1:426726/141‑1 (MQ=255)
tAAGTTTTTTGAGCTTTATAGTGTTGTTGGAGATTTAGTGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGTGTTTTTTAGATAATTATCGATTAAGTAATGTTGATTTTAATGTAACACGTTTAAGTaa                                                                                                                                           >  2:523232/1‑141 (MQ=255)
 aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                                                                                                          <  1:656757/141‑1 (MQ=255)
     tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                                      <  2:245610/141‑1 (MQ=255)
      ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGata                                                                                                                                     <  1:39596/141‑1 (MQ=255)
           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                                                                                <  2:521534/141‑3 (MQ=255)
           cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGCagaagagaagaaa                                                                                                                                >  1:265382/1‑134 (MQ=17)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGatat                                                                                                                               <  2:654569/141‑4 (MQ=14)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                               >  1:617898/1‑141 (MQ=255)
            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAGTGCGTAAAAGATATGACAt                                                                                                                               >  1:716860/1‑141 (MQ=255)
                tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                                                           <  2:799780/141‑1 (MQ=255)
                 ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                                                          <  2:136651/141‑3 (MQ=14)
                 ataGCAAGTTAAAAGATTTAATGAATTATAAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                                                          <  2:213889/141‑3 (MQ=11)
                  taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                                                                         <  2:734338/141‑1 (MQ=255)
                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacga                                                                                                                       >  1:859404/1‑136 (MQ=11)
                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGGCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                                                      <  2:222652/141‑7 (MQ=11)
                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAACAACaaa                                                                                                                      >  2:925609/1‑141 (MQ=255)
                     cAATTTTAAAGATTTAATGAACTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                                                      <  2:53097/141‑7 (MQ=11)
                         tttAAAGATTTAATGAATTACAAAGATGGAGATTTATCATATAATCCAGAGGTTCCGTGTTATTCAGCTCAATCTCAATTAACTAATGATTATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                  <  2:365894/141‑1 (MQ=17)
                         tttAAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAAGTATTCAGCTCAAAATCAATTAACAAATGATGATTGTAATGGAAAACAATTACGTCAAAGATGTGACATACAAACAaaaaaa                                                                                                                  >  2:226084/1‑141 (MQ=255)
                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  2:385343/1‑141 (MQ=255)
                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:38893/1‑141 (MQ=255)
                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:500599/1‑141 (MQ=255)
                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:256927/1‑141 (MQ=255)
                            aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                                                                               >  1:279448/1‑141 (MQ=255)
                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                             <  1:15191/141‑1 (MQ=255)
                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                             <  1:896797/141‑1 (MQ=255)
                                   taataaATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATAACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaaac                                                                                                        >  2:652223/1‑139 (MQ=255)
                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                      <  2:353805/141‑1 (MQ=255)
                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAACATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                                                                      <  1:794888/141‑1 (MQ=255)
                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                                                                     <  1:882051/141‑1 (MQ=255)
                                               aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                            >  2:349237/1‑141 (MQ=255)
                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                           >  2:587255/1‑141 (MQ=255)
                                                       gaTATATCATATAATCACGGGGTGACGCATCTTTAAGCTCAATCACACTTAAATAATGACGATTACAAGGCAAAACAATTACAAAAAATAGATTACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  2:315593/141‑1 (MQ=255)
                                                       aatatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                    <  1:385740/140‑1 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                                                                                   >  1:606642/1‑141 (MQ=255)
                                                        atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                                                                                   >  1:607557/1‑141 (MQ=255)
                                                           tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                                >  1:404150/1‑141 (MQ=255)
                                                               atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTaaa                                                                            >  2:61924/1‑141 (MQ=255)
                                                                tataATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaaa                                                                           <  1:867373/141‑1 (MQ=255)
                                                                   aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                        <  1:449599/141‑1 (MQ=255)
                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:784135/141‑1 (MQ=255)
                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:329889/141‑1 (MQ=255)
                                                                    aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                       <  2:449899/141‑1 (MQ=255)
                                                                      acAGAGAGGCCGCGTTAATTAGCTAAATATGACTGAAATAATTATGATGATAACGTAAAAAAATTACGTTAAAGTTATTTAAAACCAACAAATAAAGAAGCGAAGCAGTTGTTGAAAGGTACAGGGAATATaaacgcctac                                                                     >  2:409693/2‑134 (MQ=255)
                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGCTATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                                                                   >  2:568478/1‑141 (MQ=255)
                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGGGAATTTAAAAGGTtcatc                                                                   >  1:296759/1‑141 (MQ=255)
                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATCATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATACCAACAAATAAACAGCCGGAGCGATTGTTGAAAGGTACAGGGAATTTAAAAgatacaca                                                                   >  2:81327/1‑134 (MQ=17)
                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                                                                >  1:355534/1‑141 (MQ=255)
                                                                            gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                               >  1:630576/1‑141 (MQ=255)
                                                                             tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  2:907260/1‑141 (MQ=255)
                                                                             tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  1:697651/1‑141 (MQ=255)
                                                                             tGCCGAGTTATTCAGCACAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg                                                              >  1:524529/1‑141 (MQ=255)
                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAGAGGGAATTTAAAAGGTTCATCAGTTGGat                                                           >  1:171556/1‑141 (MQ=255)
                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGaa                                                           >  2:352391/1‑140 (MQ=255)
                                                                                   gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCAGAAGTTGGCtata                                                        >  1:398262/1‑141 (MQ=255)
                                                                                         cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                                  >  1:798485/1‑141 (MQ=255)
                                                                                          aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAATAAATAAAGCGCCGAAGAAGTTGTTGGAAGGTAGAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 >  2:704851/1‑141 (MQ=17)
                                                                                          aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                 <  2:279448/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAGAGTCATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCATTTGGATATAAAAAAATTGAAt                                            <  2:648610/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:136711/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  2:875176/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:535795/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            >  1:663116/1‑141 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  1:849179/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCACTTGGGTATAAAAAAATTGAAt                                            <  1:484207/141‑1 (MQ=255)
                                                                                               aaTATCAATTAACTAATGATGATTATAATGAAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGTTACAGGGAATTTAAAAGGTTCATCAGAAAAATAAAAAAAAATTGAAt                                            <  2:608460/141‑1 (MQ=14)
                                                                                               aaTATAAATTAACTAATGATGAGTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAt                                            <  2:444493/141‑1 (MQ=255)
                                                                                                atatCAATTAACTAATGATGATTATAATGTAAAACAATTATGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATGTaaatataggtttat                                           >  2:658042/1‑130 (MQ=14)
                                                                                                atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTAAAAGATTCATCAGTTGGATATAAAAAAATTGaaat                                           >  2:94576/1‑139 (MQ=255)
                                                                                                 tatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAttt                                          >  1:347194/1‑141 (MQ=255)
                                                                                                   tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGACGAAGCTGTTGTTGAAAGGTACAGTGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                        <  2:789041/141‑1 (MQ=255)
                                                                                                    cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACt                                       >  1:895372/1‑141 (MQ=255)
                                                                                                      aTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGGTATAAAAAAATTGAATTTACttt                                     >  1:8204/1‑141 (MQ=255)
                                                                                                       ttAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCACCCAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttt                                    >  1:220616/1‑141 (MQ=255)
                                                                                                            tAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCCCGAAACAGAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAGGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTaga                               <  1:925609/141‑1 (MQ=17)
                                                                                                            aaataatGAATAAAAAGAAAAACAAATACGTAAAAGATATAACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAAGTACAGAGAATTTAAAAGGTTCATCAGTTGAATATAAAAAAATTGAATTTACTTTTTTaga                               <  2:405531/140‑1 (MQ=17)
                                                                                                             aaTGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                              <  1:587255/141‑1 (MQ=255)
                                                                                                               tgatgaTTATAATGTAAAAAAATTACGAAGAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            >  1:935743/1‑141 (MQ=255)
                                                                                                               taatgatTATAATGTAAAACAATTACGTAAAATATATGACATACCAACAAATAAAGCGGCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:736950/139‑1 (MQ=255)
                                                                                                               aaatgatTATAATGTAAAACAATTACGTAAAAAATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTAAAATATAAAAAAATTGAATTTACTTTTTTAGAGaa                            <  2:268209/139‑1 (MQ=255)
                                                                                                                  tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                         <  1:937896/141‑1 (MQ=255)
                                                                                                                  tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                         >  2:672137/1‑141 (MQ=255)
                                                                                                                   aatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATAGAATTAACTTTTTTAGAGAATaaa                        <  1:803099/140‑1 (MQ=255)
                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  <  2:221281/141‑1 (MQ=255)
                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  >  2:398752/1‑141 (MQ=255)
                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  >  1:304094/1‑141 (MQ=255)
                                                                                                                         aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa                  <  2:404150/141‑1 (MQ=255)
                                                                                                                          aTGTAAAACCATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaa                 >  1:692197/1‑141 (MQ=255)
                                                                                                                          aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGTATAAAAATGaaa                 >  2:297533/1‑141 (MQ=255)
                                                                                                                             tAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata              <  1:349237/141‑1 (MQ=255)
                                                                                                                                aaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttt           >  2:556835/1‑141 (MQ=255)
                                                                                                                                 aCAATTACGTAAAAGATATGACATACCAACAAATAAATCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta          <  1:827900/141‑1 (MQ=255)
                                                                                                                                 aCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta          >  1:928835/1‑141 (MQ=255)
                                                                                                                                  cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttac         >  1:396527/1‑141 (MQ=255)
                                                                                                                                    aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactt       >  1:219575/1‑141 (MQ=255)
                                                                                                                                      tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  2:12508/1‑141 (MQ=255)
                                                                                                                                      tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttta     >  2:487889/1‑141 (MQ=255)
                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  2:278752/1‑141 (MQ=255)
                                                                                                                                         gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg  >  2:278744/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTG  >  CP000730/111422‑111699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: