Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,330 C→A 6.5% Q134K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,3300CA 6.5% 481.9 / 5.4 162Q134K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (77/71);  new base A (2/12);  total (79/83)
Fisher's exact test for biased strand distribution p-value = 9.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

AAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAA  >  CP000730/111190‑111470
                                                                                                                                             |                                                                                                                                             
aaaTGGCAATTCAAAACAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAAATAGAAATACAAGAAGTGCAAAAGGATTTTACTAGACAAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATc                                                                                                                                               >  1:467244/1‑141 (MQ=255)
 aaTGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑a                                                                                                                                             >  2:368219/1‑141 (MQ=255)
  aTGGCAATTCAAAATTAAGGAGAAGCTTTCAAAATAAAAGGCATGCAATTGAAGTTAGATAGAAATAAAAGAAGTGCAAAAGGATCTTACTTTACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGATCAGAATAATC‑aa                                                                                                                                            >  2:424399/1‑141 (MQ=255)
    ggCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATaaaa‑aaaa                                                                                                                                          <  2:284257/141‑1 (MQ=255)
      cAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑aaaaaa                                                                                                                                        >  1:521534/1‑141 (MQ=255)
              aaTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAGTATCCa                                                                                                                                >  1:309213/1‑141 (MQ=255)
              aaTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCa                                                                                                                                >  2:506883/1‑141 (MQ=255)
               aTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAg                                                                                                                               <  1:385779/141‑1 (MQ=255)
                 aaaGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGCAATAAAT‑ACGGACAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGtt                                                                                                                             <  1:366228/141‑1 (MQ=255)
                 aaaGGAGAAGCTATAAAAAAAAAAGGCATGCTTTTGAAGAAAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGAAAAATC‑AAAAAAAATATCCAGtt                                                                                                                             <  2:761644/141‑1 (MQ=255)
                   aGGAGAAGCTCAAAAAAAAAAAGGAATTCGTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAAAGAAATAAAG‑ACGGAGAAATTCGATGTAGTTGAAAAAAAAA‑AAAAAAAATATCCAGTTaa                                                                                                                           <  2:855818/141‑1 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:928451/1‑141 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:554136/1‑141 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:176736/1‑141 (MQ=255)
                          gCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGAtt                                                                                                                    <  1:657874/141‑1 (MQ=255)
                           ctctAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATta                                                                                                                   >  2:300397/1‑141 (MQ=255)
                              tAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATtaata                                                                                                                >  1:53097/1‑141 (MQ=255)
                              tAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAAAATCCAATACAAATTATtaata                                                                                                                >  2:888703/1‑141 (MQ=255)
                                 aaaTAAAGGGCAGGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATtaataata                                                                                                             <  1:25990/141‑1 (MQ=255)
                                 aaaTAAAAGGCATGCTTTGGAAGATAGATAGAAATAAAAGAAGTGCAAAAGGATTCTACTATACTAATGAAATAAAG‑AAGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATtaataata                                                                                                             <  2:574289/141‑1 (MQ=255)
                                  aaTAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATtaataataa                                                                                                            <  1:592315/141‑1 (MQ=255)
                                  aaTAAAACGCAATATTTATAATATAGATATAAAAACAAGAAGTGCAAAAGCATTTTACCATACTAATAAAATAAAG‑ACGGAGAGATGCGAAGTAGCTAAAAAAAAAA‑AAAAAAAATATCCAGTTAAAATGATtaataataa                                                                                                            <  2:448844/141‑1 (MQ=255)
                                    tAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAAt                                                                                                          >  2:874780/1‑141 (MQ=255)
                                            aagattttGAAGATAGAGAGAAAGACAAGAAGTGCAAAAGGATTTTACTAAACTAATGAAATAAAG‑ACGGAGAAACACGTAGTAGCTAAAAAAAAAA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCt                                                                                                  <  2:342920/137‑1 (MQ=255)
                                            aTGCTTTGTAAGATAGATACAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCt                                                                                                  <  2:194378/141‑1 (MQ=255)
                                                ttttGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTg                                                                                              >  1:631192/1‑141 (MQ=255)
                                                    gAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAAGC‑AAAAAAAATATCCAGGTAAAATGATTAATAATAAATTCATTTCTACTGAGGa                                                                                          <  2:82514/141‑1 (MQ=255)
                                                     aaGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGGAAGAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGaa                                                                                         >  1:735397/1‑141 (MQ=255)
                                                     aaGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGaa                                                                                         >  1:138263/1‑141 (MQ=255)
                                                        aaagaaaaaaaaACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACAGAGGAAGtt                                                                                      <  2:606835/133‑1 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTa                                                                                     >  1:454258/1‑141 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAA‑ACGGAGAAATAAGAAGTAGCTCAGGATAATC‑AAAAAAAATCTCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGCGGCAGTTa                                                                                     >  1:25568/1‑141 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATGTTACTAGA‑AAAAAAGCTGATGAATTGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTa                                                                                     <  1:922947/141‑1 (MQ=255)
                                                          agatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaa                                                                                    <  1:708037/141‑1 (MQ=255)
                                                          agatacaACAACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaa                                                                                    <  1:169366/141‑1 (MQ=255)
                                                           acaaaaaaTAAAAGAAGGACAAAAGGATTTTAATATAATAAAGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaaa                                                                                   <  2:25568/136‑1 (MQ=255)
                                                            atagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaag                                                                                  <  1:92015/141‑1 (MQ=255)
                                                             aagaAAAACAAGAAGTGAAAAAGGATGTTACTATACAAAAAAAATAAAG‑ACGGAGAAATACGAAGTAGCTAAAAAAAAAA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaaga                                                                                 <  2:396771/140‑1 (MQ=255)
                                                              agaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaagaa                                                                                >  2:896797/1‑141 (MQ=255)
                                                               aaaaTACAAGAAGTGCAAAAGGATAGTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaagaag                                                                               <  2:543731/140‑1 (MQ=255)
                                                                aaaTACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAATAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAagaaga                                                                              >  2:15191/1‑141 (MQ=255)
                                                                    aCAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTAATTTCTACTGAGGAAGTTAAAGAAGaaaaa                                                                          >  2:505230/1‑140 (MQ=255)
                                                                    aCAAGAAGTGCAAAAGGATTTGACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAc                                                                          <  1:769950/141‑1 (MQ=255)
                                                                     cAAGAAGTGTAAAAGAATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTAAAAATAAAA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACa                                                                         <  2:777688/141‑1 (MQ=255)
                                                                     cAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCCTTTCTACTGAGGAAGTgaaaacagaaaaca                                                                         >  2:600152/1‑141 (MQ=255)
                                                                     aaagaagTGCAAAAGGATTATACTATACTAATGACATAAAG‑ACGGAGAAATACGAAGTAGCTAAAAAAAAAA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACa                                                                         <  2:821627/140‑1 (MQ=255)
                                                                           gTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaaaa                                                                   >  2:364500/1‑141 (MQ=255)
                                                                             gCAAAAGGATTTTACTATACTAATGAAATACAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AGAAAAAATATCCAGTTAAAATACTTAATAATAAATTCATATCTCCTGAGACAGTTACAGACACACACATaaatata                                                                 >  2:883703/1‑137 (MQ=255)
                                                                             gCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaaaaaa                                                                 >  2:385740/1‑141 (MQ=255)
                                                                                 aaGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGaaa                                                             >  1:616470/1‑141 (MQ=255)
                                                                                    gATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCg                                                          <  2:555500/141‑1 (MQ=255)
                                                                                           ctatactaATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTAATGGGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACtt                                                   <  1:648026/141‑1 (MQ=255)
                                                                                           atatactaATGAAATAAAG‑ACTGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAATTATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACtt                                                   <  2:467244/140‑1 (MQ=255)
                                                                                            tatactaATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACttt                                                  <  1:318658/141‑1 (MQ=255)
                                                                                            tatactaATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACttt                                                  <  2:20414/141‑1 (MQ=255)
                                                                                             atactaATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAACAAGAAAACATAAAAAAAGAAATCGAAAACTTTa                                                 >  1:734338/1‑141 (MQ=255)
                                                                                               accaataaaataaAA‑ACGGAGGAATGCGAAGTAGCTAAAAAAAAAC‑‑AAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGt                                              <  2:79902/138‑1 (MQ=37)
                                                                                               aataataaAAAAAAG‑ACGGAGAAACACGAATTAGCTAAAAAAAAAA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAg                                               <  2:22742/139‑1 (MQ=37)
                                                                                                gcaaTGAAATAAGA‑ACAGACAAATACTAATTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATTATTAATAATAAATTCATTTCTACTGAGGAAGTTCAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGt                                              <  2:221880/139‑1 (MQ=37)
                                                                                                ctaATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAGGAAAAAAATGACTACTCAGGAAGTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGt                                              >  1:448616/1‑141 (MQ=37)
                                                                                                  aaTGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGttt                                            <  1:313748/141‑1 (MQ=255)
                                                                                                       aaTAAAG‑ACGGAGACATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTACAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTga                                       >  1:494144/1‑140 (MQ=255)
                                                                                                       aaTAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTCAGTTATTTgc                                       >  2:403061/1‑141 (MQ=255)
                                                                                                       aaTAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgc                                       <  1:579906/141‑1 (MQ=255)
                                                                                                       aaTAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgc                                       <  1:43089/141‑1 (MQ=255)
                                                                                                        aTAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcg                                      <  1:506883/141‑1 (MQ=255)
                                                                                                         aaaaT‑ACAGAAAAATACGAAATAACACAGAAAAAAA‑AAAAAAAAAAACCAGTTAAAAAGATAAAAAATAAATTCATTTATACTGAGGAAGTTAAAGAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcgc                                     <  2:617145/140‑1 (MQ=19)
                                                                                                         aaaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcgc                                     <  1:543754/140‑1 (MQ=255)
                                                                                                          aaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTCAAACGAGTAATAATATATTCATTTCTACTGAGGAAGTTAGAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:383471/1‑141 (MQ=38)
                                                                                                          aaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCa                                    >  2:136711/1‑141 (MQ=255)
                                                                                                          aaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:849179/1‑141 (MQ=255)
                                                                                                          aaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  1:376381/1‑141 (MQ=255)
                                                                                                          aaaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:484207/1‑141 (MQ=255)
                                                                                                           aaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   <  1:132697/141‑1 (MQ=255)
                                                                                                           aaG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   >  1:491833/1‑141 (MQ=255)
                                                                                                           aaG‑ACGGAGAAATACGAAGTAGCTCAGAATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   <  2:660602/141‑1 (MQ=255)
                                                                                                            aG‑ACGGAGAAATGCGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAGATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAat                                  >  1:667357/1‑141 (MQ=255)
                                                                                                            aG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAat                                  >  1:136187/1‑141 (MQ=255)
                                                                                                               aCGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAataa                                >  2:197183/1‑140 (MQ=255)
                                                                                                                cGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAAtata                               <  1:31080/141‑1 (MQ=255)
                                                                                                                cGGAGAAATACGAAGTAGCTCAGGATAATA‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAAtata                               >  1:654569/1‑141 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                             <  1:334879/141‑1 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGATCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                             <  2:124929/141‑1 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATC‑AAAACCCATATCCAGATAAAACGATTAATATTCAATTAATATATACAGAGACAGTAGTAGTACCAGATATAATAAAATTAATCTATAATTTTAAGTTTTTTTCGCAATATAGCAAt                          >  2:81933/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          >  2:449599/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          >  1:849101/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          <  1:211849/141‑1 (MQ=255)
                                                                                                                       attcGAAGTGGTTAAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAttt                        <  2:120119/138‑1 (MQ=255)
                                                                                                                       atgtgaaggtgCTTAGGATAATCAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAtt                         <  2:410226/131‑1 (MQ=37)
                                                                                                                       aTACGAAGTAGCTCAGGATAATC‑AAAAAGAATATCCAGTTAAAATGATTAATAAGAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAttt                        >  1:608741/1‑141 (MQ=255)
                                                                                                                         aCGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTa                      <  1:887741/141‑1 (MQ=255)
                                                                                                                            aaGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAg                   <  2:738128/141‑1 (MQ=255)
                                                                                                                            aaGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAg                   <  1:561644/141‑1 (MQ=255)
                                                                                                                                   tCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAAt            <  1:364500/141‑1 (MQ=255)
                                                                                                                                      ggATAATC‑AAAAACAATATCCAGTTAAAATGAATAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGaa         <  2:680331/141‑1 (MQ=255)
                                                                                                                                         aaaTC‑AAAAAAAATATCTCGTTAAAATGATTAATAATATAATCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAATACTTTAAGTTTTTTGCGCAATATAGCAATCTTAAAGATTTAATGAATta      <  1:612610/140‑1 (MQ=37)
                                                                                                                                          aaTC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATtat     <  1:502476/141‑1 (MQ=255)
                                                                                                                                           aTC‑AAAAAAAATATCCCGTTAAAATAATTAATAATAAATTCATTTCTACTGCGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGGAATATAGCAATTTTACAGATTTAATGAATtata    >  2:321558/1‑141 (MQ=37)
                                                                                                                                            tC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                        <  1:824072/40‑1 (MQ=255)
                                                                                                                                            tC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATaa   >  1:222652/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAG‑ACGGAGAAATACGAAGTAGCTCAGGATAATC‑AAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAA  >  CP000730/111190‑111470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑