Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 33.6% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT33.6% 218.1 / 138.4 165intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (50/59);  new base T (21/34);  total (71/94)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01

Only 100 of 165 total aligned reads displayed.

CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36443‑36720
                                                                                                                                          |                                                                                                                                           
cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtt                                                                                                                                           >  1:810710/1‑140 (MQ=17)
 ccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTCTATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                                                                                                                                          >  2:617021/1‑141 (MQ=17)
 ccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                                                                                                                                          >  1:588038/1‑141 (MQ=32)
   ttAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAGGACTCCTTTTTGTTAACATTATACTCTaa                                                                                                                                        >  1:74860/1‑141 (MQ=12)
      atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                                                                                                                                     <  1:754541/141‑1 (MQ=34)
      atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                                                                                                                                     <  2:367354/141‑1 (MQ=35)
       aatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATACTATAAATTc                                                                                                                                    <  2:641276/140‑1 (MQ=25)
           actgATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGTAGACTCCTGTTTGTTAAAATTATACTATAAATTCAACt                                                                                                                                <  1:187929/138‑1 (MQ=255)
              gATATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAATTTCAACTTTg                                                                                                                             <  1:683885/141‑1 (MQ=255)
               atatatCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGCGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGc                                                                                                                            <  1:587874/141‑1 (MQ=255)
               atatatCACGATAGCTCAATGGATCTCATGGAGAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGc                                                                                                                            >  1:456209/1‑141 (MQ=11)
                 atatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATATCATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCaa                                                                                                                          <  1:538986/141‑1 (MQ=25)
                  tatCACGATAGCTCAATGTATATATTAGTTAGTAGCCAACGGCTACGGTGATAACATCCTTGTTAAATTGTTGATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCGACTTTGCAAc                                                                                                                         <  2:184198/141‑1 (MQ=11)
                    tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg                                                                                                                       <  1:215630/141‑1 (MQ=35)
                     cACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCATTTTTGTTAAAATTATACTATAAATTCAACTTTCCacccga                                                                                                                      >  1:116292/1‑136 (MQ=255)
                          ttgcttaaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  2:582/135‑1 (MQ=255)
                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:868682/138‑1 (MQ=9)
                          aagcctaaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  2:324286/135‑1 (MQ=255)
                           agcTCAATTCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                                                                                                                <  2:598123/139‑1 (MQ=255)
                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  2:828061/1‑141 (MQ=38)
                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  2:271117/1‑141 (MQ=38)
                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               <  1:817193/141‑1 (MQ=38)
                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTAATTTTATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              >  2:882925/1‑141 (MQ=11)
                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              >  1:788610/1‑141 (MQ=12)
                              tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                             >  1:378210/1‑141 (MQ=40)
                              tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTATTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                             <  2:502011/141‑1 (MQ=255)
                              tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                             >  1:77696/1‑140 (MQ=16)
                                aattgatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATg                                                                                                           <  2:589180/136‑1 (MQ=255)
                                aaaccatatCTTAAATATTAGCCAACGACTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTTCATACAGAATACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaact                                                                                                           <  2:857539/136‑9 (MQ=11)
                                aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttt                                                                                                           >  2:469023/1‑138 (MQ=255)
                                 atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAACGtattttat                                                                                                          >  1:372918/5‑137 (MQ=255)
                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  2:895401/139‑7 (MQ=255)
                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                       <  1:483229/140‑8 (MQ=255)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      <  1:124551/141‑1 (MQ=255)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTGTATCTGAAATAGTTCATACAGAAGACTCCTTTATGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagt                                                                                                      >  2:519331/1‑133 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTAAATTATATTATAAATTCAACTTTGCAACAGAAcatcctaactgtatt                                                                                                      >  2:586003/1‑127 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                                      <  2:309778/141‑14 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                                                                                                      <  2:650150/141‑14 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      <  2:795137/141‑1 (MQ=255)
                                     atatCTTAGATAGTAGCCAACGGCCACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      <  1:280175/141‑1 (MQ=255)
                                      tatGTTAGATAGTAGACAACGGCTACAGTGATAACATCGTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                     <  2:726337/141‑10 (MQ=11)
                                      tatCTTAGCTGTTATCCAACAGCTACAGTGATTATTCCCGTGTTCAATTGTTTCTTGCACAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     >  2:429497/1‑127 (MQ=2)
                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                     <  1:235621/141‑10 (MQ=255)
                                      tatCTTAGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATAGCTGAAATAGTTCATACAGAAGACTCCTTTTGGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaatggtatta                                                                                                     >  2:604355/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     >  1:299330/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     <  2:128552/141‑15 (MQ=11)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     <  1:774077/141‑15 (MQ=11)
                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATCGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACAGAATTATGGagtcgg                                                                                                    >  1:88905/1‑136 (MQ=255)
                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATATTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                                    <  2:191263/141‑11 (MQ=11)
                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                                    >  2:166759/1‑141 (MQ=255)
                                        tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca                                                                                                   >  1:929303/1‑130 (MQ=11)
                                        tCTTACATAGTTGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACATAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca                                                                                                   <  2:771740/141‑12 (MQ=11)
                                          ttAGATAGTAGCCAACGGCTAGAGTGATAACATCCTTGTTAATTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                                 <  2:4924/141‑14 (MQ=11)
                                          ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                                 >  1:300190/1‑128 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaccctc                                                                                                >  2:764358/1‑127 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:124090/141‑15 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:584003/141‑15 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  1:351240/141‑15 (MQ=11)
                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:924246/141‑15 (MQ=11)
                                            aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACCGAACCGAATTATGGAATAGAGATGt                                                                                               >  1:301825/1‑141 (MQ=255)
                                             gatatgcgCCAACGGTCACAGTGATAACATCCTGGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTTTGTTAACCTTATACACCAAATCCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGtt                                                                                              <  2:387371/134‑1 (MQ=17)
                                                  tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                         >  2:761238/1‑141 (MQ=255)
                                                  tagCCAACCGCTACAGTTATAACATCTTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                         <  1:417290/141‑1 (MQ=255)
                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                        >  2:753232/1‑141 (MQ=255)
                                                     ccAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAt                                                                                      >  1:46708/1‑141 (MQ=255)
                                                      cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                     >  2:328005/1‑141 (MQ=255)
                                                      cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                     <  1:563336/141‑1 (MQ=255)
                                                              aCAGTGATAACATCCTTGTTAAATAGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAgg                                                                             >  1:688080/1‑141 (MQ=255)
                                                               cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCCACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGa                                                                            <  1:844713/141‑1 (MQ=255)
                                                               cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGa                                                                            <  1:503502/141‑1 (MQ=255)
                                                                   gATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCAt                                                                        >  1:799195/1‑141 (MQ=255)
                                                                     tAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATta                                                                      >  2:805616/1‑141 (MQ=255)
                                                                            cTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTaa                                                               <  1:256505/141‑1 (MQ=255)
                                                                                   aaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAt                                                        <  1:104841/141‑1 (MQ=255)
                                                                                    aTTGTTTATATCTGAAATAGTTCATACAGGAGACTCCTTTTTGTTAAAGATTTAATGCGGATTAAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                       <  1:643137/141‑1 (MQ=25)
                                                                                    aTTGTTTATAGTGGAAAGAGTTCAGACAGAAGGCTACTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                       <  1:894903/141‑1 (MQ=25)
                                                                                     ttGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAttt                                                      <  1:291693/141‑1 (MQ=255)
                                                                                              tCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTAc                                             >  1:791969/1‑141 (MQ=255)
                                                                                               cTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACa                                            >  1:701813/1‑141 (MQ=255)
                                                                                                tGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAg                                           >  2:381389/1‑141 (MQ=255)
                                                                                                tGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATATACTTAAGTTTGATTTCGTTATTACAGa                                          <  2:812188/141‑1 (MQ=255)
                                                                                                 gAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGa                                          >  1:808055/1‑141 (MQ=255)
                                                                                                  aaaTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAACTCAACTTTGCACCAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGaa                                         <  2:505724/141‑1 (MQ=255)
                                                                                                       gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                                    >  2:835899/1‑141 (MQ=255)
                                                                                                          cATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACCCAt                                 >  2:810896/1‑141 (MQ=255)
                                                                                                           aTACAGAAGACTCCTTTTTGTTTAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACAtt                                >  2:688112/1‑141 (MQ=255)
                                                                                                            tACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacaccgtc                               <  2:18119/141‑5 (MQ=255)
                                                                                                                gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAGTTCTACCGGATCATTATACTTAAGTTTACTTTGGTTATTACAGAATCCCACCTTCAAAc                           >  1:320428/1‑141 (MQ=37)
                                                                                                                   gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                        >  1:142518/1‑141 (MQ=255)
                                                                                                                    cctctTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAACTTTATATTCCTTATTACAGAACCACACATTCCAACCaga                       <  1:224637/140‑1 (MQ=38)
                                                                                                                    aCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                       <  2:799195/141‑1 (MQ=255)
                                                                                                                    aCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                       <  1:753232/141‑1 (MQ=255)
                                                                                                                     cTCCTTTGTGGTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTATAATTTCGTTATTACAGAACCACACATTCCAACCagaa                      <  1:134463/141‑1 (MQ=255)
                                                                                                                         tttttGTTAAAATTATTCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                  <  1:100273/141‑1 (MQ=255)
                                                                                                                         tttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCCTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTGCAGAACCACACATTCCAACCAGAAgaga                  >  1:534939/1‑141 (MQ=255)
                                                                                                                            ttGTTAAAATTACACTATAAATTCAACCTGGCAACAGACCCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAg               <  2:868803/141‑1 (MQ=255)
                                                                                                                               ttAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATATAGGATCATTATACTTAAGTTTAATTTCGTTATTGCAGAACCACACATTCCAACCAGATGGGGAAGTAt            >  1:344030/1‑141 (MQ=39)
                                                                                                                                     ttATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAt      <  1:48257/141‑1 (MQ=255)
                                                                                                                                      tataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAtt     <  1:437981/141‑1 (MQ=255)
                                                                                                                                         aCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAg  >  1:840825/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36443‑36720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: