Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,605,313 T→G 4.0% intergenic (‑107/+345) USA300HOU_2465 ← / ← USA300HOU_2466 possible decarboxylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,605,3130TG 4.0% 302.7 / 3.4 99intergenic (‑107/+345)USA300HOU_2465/USA300HOU_2466possible decarboxylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (50/45);  new base G (2/2);  total (52/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 102 total aligned reads displayed.

ACTCTCCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTCAATAAATC  >  CP000730/2605173‑2605449
                                                                                                                                            |                                                                                                                                        
aCTCTCCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCtt                                                                                                                                          <  2:699578/141‑1 (MQ=255)
 ctctCCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTGCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCttt                                                                                                                                         >  2:502040/1‑141 (MQ=255)
 ctctCCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCttt                                                                                                                                         >  1:39842/1‑141 (MQ=255)
   ctcCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAg                                                                                                                                       <  2:196601/141‑1 (MQ=255)
   ctcCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAg                                                                                                                                       >  1:649173/1‑141 (MQ=255)
    tcCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGt                                                                                                                                      <  1:819158/141‑1 (MQ=255)
    tcCATTTTGTTACTTGTAATTAGACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGt                                                                                                                                      <  2:147084/141‑1 (MQ=255)
     tcATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCCACTTCACATAGTTAAATACATATCCAGTCATACTACACGTCGAACAATATTGGATACAATTTTAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGtt                                                                                                                                     <  2:378706/140‑1 (MQ=255)
       aTTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTaa                                                                                                                                   <  2:357019/141‑1 (MQ=255)
            gTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACAt                                                                                                                              >  2:387952/1‑141 (MQ=255)
            gTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACAt                                                                                                                              >  2:348889/1‑141 (MQ=255)
             tttCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACAtt                                                                                                                             >  1:663778/1‑141 (MQ=255)
               tCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTa                                                                                                                           >  2:70860/1‑141 (MQ=255)
               tCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTa                                                                                                                           >  1:541429/1‑141 (MQ=255)
                      aTTATACGTCATTTGCAATCTCTCCTGTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGCATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTta                                                                                                                    <  2:679943/141‑1 (MQ=255)
                        tataCGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTtata                                                                                                                  <  1:252455/141‑1 (MQ=255)
                         ataCGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTatat                                                                                                                 <  1:387071/141‑1 (MQ=255)
                          taCGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATAtt                                                                                                                >  1:817868/1‑141 (MQ=255)
                            cGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAACCATTAACCACTTATATTTa                                                                                                              >  2:657797/1‑141 (MQ=255)
                             ggCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTaa                                                                                                             <  1:864886/141‑1 (MQ=255)
                               cATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAATAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTaaaa                                                                                                           >  1:568820/1‑141 (MQ=255)
                                             ccTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAatat                                                                                             <  1:576082/141‑1 (MQ=255)
                                              cTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATg                                                                                            >  1:237181/1‑141 (MQ=255)
                                              cTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATg                                                                                            >  1:67231/1‑141 (MQ=255)
                                              cTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATg                                                                                            >  1:98101/1‑141 (MQ=255)
                                               tttACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGa                                                                                           >  1:786344/1‑141 (MQ=255)
                                                 tACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGaaa                                                                                         >  2:683960/1‑141 (MQ=255)
                                                 tACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGaaa                                                                                         >  2:822533/1‑141 (MQ=255)
                                                   cATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAAc                                                                                       <  1:458346/141‑1 (MQ=255)
                                                     ttGTTTAATGCCAATCCTGTGATACTACACTTCGAACAATATTGGATACAATTTGAAATTCTGTATAACATCTTGTCAATCTATTCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAAGATGAAAACAg                                                                                     <  2:406540/141‑1 (MQ=255)
                                                     ttGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAg                                                                                     >  1:744017/1‑141 (MQ=255)
                                                     ttGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAg                                                                                     >  1:903480/1‑141 (MQ=255)
                                                      tGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAAACTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGa                                                                                    <  2:181667/141‑1 (MQ=255)
                                                       gTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGAt                                                                                   >  2:709957/1‑141 (MQ=255)
                                                       gTTTAATACCAAGCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGAt                                                                                   <  1:403066/141‑1 (MQ=255)
                                                        tttAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGGATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGAtt                                                                                  >  1:181837/1‑141 (MQ=255)
                                                        tttAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGGtt                                                                                  >  1:74027/1‑141 (MQ=255)
                                                        tttAATACCAATCCAGTGAGTCTACACTTCGAACAATATTGGATAGAATTTGAACTTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCGCTTATATTTAAAAAATATATAAAATATGAAAACAGAtt                                                                                  >  1:612577/1‑141 (MQ=255)
                                                        tttAATACCAATCAAGTGATACTACACTTCGAACAATATTGGATAGAAGTTTAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGAtt                                                                                  >  2:643654/1‑141 (MQ=255)
                                                         ttAATACCTATCCAGTGATACTCCACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTg                                                                                 <  1:537311/141‑1 (MQ=255)
                                                         ttAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAGAATATATAAAATATGAAAACAGATTg                                                                                 <  2:585590/141‑1 (MQ=255)
                                                             tACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATGTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAg                                                                             >  2:325202/1‑141 (MQ=255)
                                                              aCCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAACATATATAAAATCTGAAAACAGATTgcacgg                                                                            >  1:660735/1‑136 (MQ=255)
                                                              aCCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAgg                                                                            <  1:110668/141‑1 (MQ=255)
                                                                cAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGat                                                                          >  2:908819/1‑141 (MQ=255)
                                                                 aaTCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGata                                                                         >  1:494218/1‑141 (MQ=255)
                                                                  aTCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGatat                                                                        >  1:525838/1‑141 (MQ=255)
                                                                       gggATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACAAATTGTCAATCTATTCTTTAGTTAAAACATTTAGCACTTATAGTTAAAAAATATATAAAATATGAAAACTGATTGAAAGGATATTATAc                                                                   <  2:49435/139‑1 (MQ=255)
                                                                       gTGATACTACACTTCGAACTATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAGAAAATATATAAAATATGAAACATGATTCAAAGGAtattattc                                                                   >  1:191782/1‑139 (MQ=255)
                                                                       gTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAc                                                                   >  1:779428/1‑141 (MQ=255)
                                                                       gTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAc                                                                   >  1:245575/1‑141 (MQ=255)
                                                                       gTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAc                                                                   <  2:31256/141‑1 (MQ=255)
                                                                       gTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATGTAGCACTTATATTTAAAAAATATATAAAATATGCACACCAATCGAAAGCatactcgac                                                                   >  2:838967/1‑135 (MQ=255)
                                                                        tGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAcc                                                                  >  2:619092/1‑141 (MQ=255)
                                                                        tGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAcc                                                                  <  2:560122/141‑1 (MQ=255)
                                                                        ggATACTACACTGCGAACAATATTAGATAGAATTTGAAATGCTGCATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATAcc                                                                  <  2:481862/140‑1 (MQ=255)
                                                                         gATACTACTCTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCt                                                                 <  2:544667/141‑1 (MQ=255)
                                                                         gATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCt                                                                 <  1:846085/141‑1 (MQ=255)
                                                                               acacTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTGTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGctct                                                           >  2:558471/1‑141 (MQ=255)
                                                                               acacTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTGTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGctct                                                           >  2:375884/1‑141 (MQ=255)
                                                                                cacTTCAGACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTa                                                          <  1:609717/141‑1 (MQ=255)
                                                                                 acTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAc                                                         <  1:865277/141‑1 (MQ=255)
                                                                                 acTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAc                                                         <  2:578468/141‑1 (MQ=255)
                                                                                 acTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAc                                                         <  1:655725/141‑1 (MQ=255)
                                                                                 acTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAc                                                         <  2:649173/141‑1 (MQ=255)
                                                                                 acTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAc                                                         <  1:727774/141‑1 (MQ=255)
                                                                                      gAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCttt                                                    <  1:470421/141‑1 (MQ=255)
                                                                                      caaCAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCttt                                                    <  1:251902/140‑1 (MQ=255)
                                                                                       aaCAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCtttt                                                   <  1:790137/141‑1 (MQ=255)
                                                                                       aaCAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCtttt                                                   <  2:148553/141‑1 (MQ=255)
                                                                                        aCAATATTGGATAGAATTTGAAATTCTGTATAACAGCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCttttt                                                  <  2:34439/141‑1 (MQ=255)
                                                                                           atatTGGAGAGAATTTGAAATTCTGTATAACATCTTGACAATCTATGCTTTAGTTAAAACATTTAGCAAAAATAAAAAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTtat                                               <  2:308695/141‑1 (MQ=255)
                                                                                             atTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATaa                                             >  1:401772/1‑141 (MQ=255)
                                                                                             atTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATaa                                             <  2:698995/141‑1 (MQ=255)
                                                                                                   tAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACAGTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATc                                       >  1:646737/1‑141 (MQ=255)
                                                                                                    aGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATca                                      <  2:744017/141‑1 (MQ=255)
                                                                                                    aGAATTTGAAATTCTGTATAACATCTTGTCAATCTAAGCTTTAATCAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATca                                      <  2:903480/141‑1 (MQ=255)
                                                                                                       aTTTCAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATcacac                                   >  2:384947/1‑141 (MQ=255)
                                                                                                         ttGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTTTAAAAAATtaaacaa                                 >  2:86206/1‑140 (MQ=255)
                                                                                                         ttGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTTTAAAAAATcacaccc                                 >  2:805198/1‑139 (MQ=255)
                                                                                                         ttGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATcacacac                                 >  2:50810/1‑141 (MQ=255)
                                                                                                         ttGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATcacacac                                 >  2:322888/1‑141 (MQ=255)
                                                                                                         ttGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATcacacac                                 >  1:415140/1‑141 (MQ=255)
                                                                                                           gAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACAt                               <  1:302515/141‑1 (MQ=255)
                                                                                                             aaTTCTTAATAATATCTTGTCAATTTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAATTATGAAAACTGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCTCACACAttt                             <  2:749212/141‑1 (MQ=255)
                                                                                                              actCTGCAAAACAACTAGTCAATCTAAGCTTTAGTTAAAACATTTAGCACGTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTAAAAAAAAAAAAAAAAAATCACACACATTTg                            <  2:646737/139‑1 (MQ=255)
                                                                                                              aTTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTg                            <  1:792447/141‑1 (MQ=255)
                                                                                                                 cTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTgaga                         <  2:786344/141‑1 (MQ=255)
                                                                                                                     ataACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGTACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATTAAAAATCATACACATTTGAGAAAAt                     >  2:613425/1‑141 (MQ=255)
                                                                                                                        aCATCTTGTCAATCTATGCTTTAGTTCAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTg                  >  2:584825/1‑141 (MQ=255)
                                                                                                                        aCATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTg                  >  2:462398/1‑141 (MQ=255)
                                                                                                                          aTCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGAt                <  2:3880/141‑1 (MQ=255)
                                                                                                                            cTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATaa              >  1:432512/1‑141 (MQ=255)
                                                                                                                               gTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTCATAAtat           >  2:410448/1‑139 (MQ=255)
                                                                                                                                tCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTc          >  1:669007/1‑141 (MQ=255)
                                                                                                                                tCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTc          >  1:669201/1‑141 (MQ=255)
                                                                                                                                   aTCTATGCTGTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTCAAt       <  1:375884/141‑1 (MQ=255)
                                                                                                                                   aTCTATGCTGTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTCAAt       <  1:558471/141‑1 (MQ=255)
                                                                                                                                        tGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAGTGTCAATAAATc  >  2:485761/1‑141 (MQ=255)
                                                                                                                                        tGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTCAATaaacc  >  2:630758/1‑139 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ACTCTCCATTTTGTTTCTTGTAATTATACGGCATTTGCAATCTCTCCTTTACATTGTTTAATACCAATCCAGTGATACTACACTTCGAACAATATTGGATAGAATTTGAAATTCTGTATAACATCTTGTCAATCTATGCTTTAGTTAAAACATTTAGCACTTATATTTAAAAAATATATAAAATATGAAAACAGATTGAAAGGATATTATACCTTGCTCTACCCTTTTTTATAAAAAATCACACACATTTGAGAAAATTTGATAATGTCAATAAATC  >  CP000730/2605173‑2605449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: