Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,504,989 T→C 8.8% intergenic (+293/+111) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,504,9890TC 8.8% 296.1 / 7.0 142intergenic (+293/+111)USA300HOU_2365/gltThypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base T (59/63);  new base C (3/15);  total (63/79)
Fisher's exact test for biased strand distribution p-value = 1.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

TTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504850‑2505129
                                                                                                                                           |                                                                                                                                             
ttGCGCATGCATAAACGCCCCTTCCAACCTGAGGTCACTGCGCTTCCGTTCGGCTGGGCTTTAGCGCATGCATAAAAGCCCCTAACACCCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTc                                                                                                                                             <  2:36124/141‑1 (MQ=14)
  gcgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTccc                                                                                                                                           >  2:755482/1‑141 (MQ=255)
   cgctcgcatTAACGCCCCTATCATCCTGAGGCCACCCCGCGCCGGTTCGCCTGCGCTTTAGCGCATGCATAAACGCCCCTAACACCCGGAGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCt                                                                                                                                          <  1:104779/136‑1 (MQ=14)
   cgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCt                                                                                                                                          <  1:562407/141‑1 (MQ=255)
    gcatgcatCAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTa                                                                                                                                         <  1:141176/141‑1 (MQ=255)
     catgcatAAGAGCCCCTATCCACCTGGGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaa                                                                                                                                        <  2:571479/141‑1 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCGCTACGCGCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaa                                                                                                                                        >  1:770940/1‑141 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaa                                                                                                                                        >  1:396236/1‑141 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaa                                                                                                                                        >  1:276673/1‑141 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCATTAGCACATGCATAAAAGCCCCTAACAACCGGAGGTCACTACGCGTCGGGTCGCCTCCGCATTACCGCACACATAAAAACCCCTaa                                                                                                                                        >  2:242204/1‑141 (MQ=11)
      aagcatTAAAGCCCCTAACAACCTGAGTGCACGTCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaac                                                                                                                                       >  2:935948/3‑141 (MQ=14)
        gcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaacaa                                                                                                                                     >  1:887676/1‑141 (MQ=255)
               aGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAgg                                                                                                                              >  1:284060/1‑141 (MQ=255)
                     tAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTa                                                                                                                        >  1:68076/1‑141 (MQ=255)
                             tGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTTCGCTTcct                                                                                                                >  2:295491/1‑140 (MQ=255)
                              gcggTCACCCCCCTCCGGTTCGCCCGCGCGTTGCCGCATGCATAAACGCCCCTACCAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGt                                                                                                               <  2:565273/139‑1 (MQ=14)
                               gggTCCCTGCGCCCGCGTTCGCCTGCGCTTCAGCGCATGCATAAAAGCCCCTAACAACCGTAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAACAGCCCCAATCAACCTGAGGTCACTACGCTTCCGtt                                                                                                              <  1:224855/140‑1 (MQ=14)
                               aGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGCGGTCACTAAGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGTTCACTGCGCTTCCGtt                                                                                                              >  1:102218/1‑141 (MQ=255)
                               aGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGtt                                                                                                              <  1:446184/141‑1 (MQ=255)
                               aGGTCACTGCGCTCCAGTTCGCCTGGGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGtt                                                                                                              >  1:472849/1‑141 (MQ=255)
                                ggTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTc                                                                                                             >  1:899205/1‑141 (MQ=255)
                                ggTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTAACTACGCTTCCGTTc                                                                                                             >  1:804462/1‑141 (MQ=255)
                                      tGCGCCCCGGTTCGCCTGCGCTTTAGCCCACGCATAAAAGCCCCTAACCTCCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgc                                                                                                       <  2:465593/141‑1 (MQ=17)
                                       ccgcTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcg                                                                                                      <  1:672279/140‑1 (MQ=255)
                                          cTCCGGTTCGCCTGCGCTTTCGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGATTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt                                                                                                   <  2:702620/141‑1 (MQ=255)
                                          cTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCGCTAACAACCTGAGGTAACTACGCTTCCGTTCGCCTGCGCtt                                                                                                   >  1:530784/1‑141 (MQ=255)
                                          cTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACGACGCTTCCATTCGCCCgcgcat                                                                                                   >  2:27778/1‑139 (MQ=255)
                                           cccgttccGCCTCCGCTTCGCGCATGCATACAAGCCCCTAACAACCCGAGGTCACTACGCTTCGGTTCGCCCGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTa                                                                                                 <  2:294339/134‑1 (MQ=11)
                                               gTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAgcg                                                                                              >  1:461025/1‑141 (MQ=255)
                                                 tCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCCCCTGCGCTTTAGCgca                                                                                            <  1:485205/141‑1 (MQ=255)
                                                    ccTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTCCCGTTCGCCTGCGCTTTAGGgcatga                                                                                         >  2:736909/1‑140 (MQ=255)
                                                    ccTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCGTCAGTTCGCCTGCGCTATAGCgcatgc                                                                                         >  2:738029/1‑141 (MQ=255)
                                                      tttgcTTTAGCGCATGCATAAAAGCACCCACCAATCTNAAATCCCTACGCTACGGTTCCCCTGCTCTTTAGCGCATGCATAAACGTCCCTAACAACCAGAGGTCACTACGCTACCGTTCGCCTGCGCTTTAGCgcatgtct                                                                                       <  2:161221/138‑4 (MQ=14)
                                                      tGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                       <  1:451068/141‑1 (MQ=255)
                                                      tGCCCTTTAGCGCATTCATAAATGCCCCTAACAACCGGGGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgcat                                                                                       <  2:900730/141‑1 (MQ=14)
                                                        cgcTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTTCGCATTAGCGCTTGCATaa                                                                                     >  2:260159/1‑141 (MQ=255)
                                                          cTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATaaaa                                                                                   <  1:395973/141‑1 (MQ=255)
                                                               gcgcATTCATAAAAGCCCCTTACAACCTGAGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGcccc                                                                              <  2:873555/141‑1 (MQ=255)
                                                                cgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTGTAGCGCATGCATAAAAGCCCCt                                                                             >  1:652299/1‑141 (MQ=255)
                                                                 gcatgcatAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTa                                                                            >  1:499983/1‑141 (MQ=255)
                                                                     gcatAAAAGCCCCTAACAACCTGAGGGCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                        <  1:97084/141‑1 (MQ=255)
                                                                     gcatAAAAGCCCCTAACAACCGGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                        <  1:359880/141‑1 (MQ=255)
                                                                       atAACAGCCCCTTAGAATCTGAGGGCACTACGCTTCGGCTCCACTGCGCTTTAGCGCATGCATAAATGTCCCTAACAGCCTTAGTTCACGACGCTTCCGTTCGCCTGCGCTTTATCGCATGCATAAAAGCCCCTAACAAcc                                                                      <  2:564971/141‑1 (MQ=14)
                                                                        tAAAAGCCCCTAACAACCTGTGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCa                                                                     >  1:569868/1‑141 (MQ=255)
                                                                          aaaGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCaaa                                                                   >  1:356132/1‑141 (MQ=255)
                                                                           atcccccTACAACCTGAGGTCACACCGCTTCGGATCCCCTTCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAgg                                                                 <  2:532249/138‑1 (MQ=14)
                                                                           aaGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAACCCCCTAACAACCAAAg                                                                  <  1:469751/141‑1 (MQ=255)
                                                                                ccTAACAGTCTGAGGTCACTACGCTTCGGTTCGCCTTCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                             <  2:258158/141‑1 (MQ=255)
                                                                                ccTAACAACCTGAGGTCACTACGCTTCGGTTCTCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCGCTGCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAGATGCTCCTGACCATCGACGGTTGt                                                             >  2:201790/1‑141 (MQ=14)
                                                                                ccTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                             >  2:131457/1‑141 (MQ=255)
                                                                                acTAACAACCGGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTACCGTTCGCCTGCGCTTTACCGCTTACATAAAAGCCCCTAACTACCAACGATTgg                                                             >  2:817402/2‑140 (MQ=14)
                                                                                 cTAACCACCTGTGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTa                                                            <  2:774622/141‑1 (MQ=255)
                                                                                 cTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTa                                                            <  2:164849/141‑1 (MQ=255)
                                                                                  caacaccCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTaa                                                           <  2:502361/140‑1 (MQ=255)
                                                                                  caacaacCTGAGGTCCCTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTaa                                                           <  2:818320/140‑1 (MQ=255)
                                                                                   aacaacCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTAGGCATTAGCGCATGCATCAAAGCCCCTAACAACCCAAGGTTGTAAg                                                          >  2:326801/1‑141 (MQ=17)
                                                                                    acaacCTGACGTCCATACGCTTCGGTTCGCCTGAGCTCTAGCGCATGCATAAAAGACCCTAACAACCTGAGGTCACTACGCGTCCGAACGCCTACCCATTAGCGCATGCATCAAACCCACAGACAACTAAACGTTGtcgtt                                                         >  2:672671/1‑137 (MQ=9)
                                                                                     caacCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAggg                                                        <  1:727929/141‑1 (MQ=255)
                                                                                       acCTGGGGTCACTCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGGCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGc                                                      <  2:704852/141‑1 (MQ=255)
                                                                                        ccTGGGGTCACGCCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCt                                                     <  2:499452/141‑1 (MQ=255)
                                                                                         cTGTGGTCACTACGCTTCGGTTCGCCTGCGGCTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCtt                                                    <  2:597447/141‑1 (MQ=255)
                                                                                         cTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAAACTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTACCAACCAAAGGTTGTAAAGGGCtt                                                    >  1:945109/1‑141 (MQ=255)
                                                                                             ggTCCCTACGCTTCGGTTCGGCCGCGCTTTAGCGAATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCGTCCGTTCGCCTGCGCTGTAGCGCAGGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTaaa                                                <  1:246779/141‑1 (MQ=17)
                                                                                             ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTATCGCATGCATAGAGGCACCAACCAACCACAGGTGGTAAGGGGCTTTaaa                                                >  1:357963/1‑141 (MQ=17)
                                                                                             ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTaaa                                                >  1:454025/1‑141 (MQ=255)
                                                                                             ggTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTaaa                                                >  1:808779/1‑141 (MQ=255)
                                                                                              gtcatcccGCTGCCGTTCGTCTGCGCTTTTGCGCATGCATCAGAGTCCCTGTCATCCTGAGGTCACTACGCTGCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAAt                                               <  2:643364/134‑1 (MQ=17)
                                                                                                cACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGt                                             <  1:285884/141‑1 (MQ=255)
                                                                                                cACTACGCTCCGGTCGCCCTTCGCTTTAGCGCATGCATAAAAGTCCCTAACACCCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGt                                             <  1:876938/141‑1 (MQ=18)
                                                                                                 aCTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTc                                            >  1:793291/1‑141 (MQ=255)
                                                                                                  cTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCa                                           >  1:104063/1‑141 (MQ=255)
                                                                                                          cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTTCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAt                                   <  1:603345/141‑1 (MQ=255)
                                                                                                          cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAt                                   <  1:272364/141‑1 (MQ=255)
                                                                                                            gTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAttt                                 >  1:741287/1‑141 (MQ=255)
                                                                                                            gTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAttt                                 >  1:107073/1‑141 (MQ=255)
                                                                                                            gTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAttt                                 >  1:107095/1‑141 (MQ=255)
                                                                                                               ccccTGCGCTTTAGCTCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtgtttt                              <  2:575945/139‑1 (MQ=255)
                                                                                                               cGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              <  1:755482/141‑1 (MQ=255)
                                                                                                                gCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTa                             <  1:131457/141‑1 (MQ=255)
                                                                                                                  cTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaaa                           <  2:45188/141‑1 (MQ=255)
                                                                                                                    gcgcTAAAGCGCAGACATAGAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATCAAAGCCCCTAACAACCAAAGGTCGTAAGGGGCTTTAAATGTCCGATAGTATTTTTTAAGtt                         >  1:614274/1‑141 (MQ=21)
                                                                                                                     cgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTg                        >  2:787590/1‑141 (MQ=255)
                                                                                                                         ttAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTCAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGAttt                    >  2:515186/1‑141 (MQ=255)
                                                                                                                           tgcgcATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  <  2:770940/140‑1 (MQ=255)
                                                                                                                           aGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  <  2:396236/141‑1 (MQ=255)
                                                                                                                           aGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  <  1:242204/141‑1 (MQ=255)
                                                                                                                           aGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  <  2:276673/141‑1 (MQ=255)
                                                                                                                            gcgcATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTaa                 >  2:689238/1‑141 (MQ=255)
                                                                                                                             cgcATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCGTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTaaa                >  2:707634/1‑141 (MQ=255)
                                                                                                                                 tgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATAtt            >  2:774552/1‑141 (MQ=255)
                                                                                                                                    atAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCtt         >  2:33727/1‑141 (MQ=255)
                                                                                                                                    atAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCtt         >  2:933948/1‑141 (MQ=255)
                                                                                                                                    atAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGCTGTGTTTTATTTTAAAAGACGGGTAGTGTTTTTTAAATTGATTTTAAATATTCtt         >  1:443676/1‑141 (MQ=17)
                                                                                                                                    atAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCtt         <  2:215745/141‑1 (MQ=255)
                                                                                                                                      aaaaGTGCCCTAACATCCTGAGGGCACGACGCTTCCGGTCGGCTTCGCTTTAGCGCATGCATAAAAGCCCCGAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg        <  1:902306/141‑1 (MQ=37)
                                                                                                                                      aaaaGTCCCCAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  1:647008/141‑1 (MQ=255)
                                                                                                                                      aaaaGCCCCTAACAACCTGCGGTCACTACGCGTCCGTTCGCCTGAGATTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  2:614274/141‑1 (MQ=37)
                                                                                                                                      aaaaGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  2:447421/141‑1 (MQ=255)
                                                                                                                                        aaGCCCCTAACCACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGAcc     <  2:42697/141‑1 (MQ=255)
                                                                                                                                           tCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCACATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCttt  <  1:672379/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
TTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504850‑2505129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: