BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	57.7%	E194E (GAA→GAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	57.7%	436.1 / inf	830	E194E (GAA→GAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (181/166); new base C (220/252); total (408/422)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

Only 100 of 832 total aligned reads displayed.

TAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAA  >  CP000730/35847‑36108

aaatcACTAATATATCATGGCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGt                                                                                                                           <  2:234206/137‑1 (MQ=1)

                      gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                     >  2:622575/1‑141 (MQ=255)

                         ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGa                                                                                                  >  1:148175/1‑141 (MQ=12)

                         ttCGTTGAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGTGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTgt                                                                                                  >  2:694589/1‑140 (MQ=11)

                          tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                 >  2:727684/1‑141 (MQ=32)

                          tCGCGTAACTTGCTTACATGATGTTAAAGTCGATACCTGGCGAAAATCCGTAGATCTGAACAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                 <  2:639188/141‑1 (MQ=11)

                            gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                               <  1:1074327/141‑1 (MQ=17)

                             tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                              <  2:131521/140‑3 (MQ=255)

                              ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                                             <  1:934534/141‑4 (MQ=255)

                               tAACTTGCTAGCATGATGCTGATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCTATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtg                                                                                            >  1:776448/1‑140 (MQ=11)

                               tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                            <  1:185703/141‑1 (MQ=32)

                                aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                           <  1:744578/141‑1 (MQ=32)

                                 acgtgccggCATGATGTTAAGTCCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                          <  2:1023077/133‑1 (MQ=11)

                                 aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAGATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                          >  1:468603/1‑141 (MQ=17)

                                 aCTTGCTAGCATGATGCTAATTGCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTATACTATGCTCTGCCGTATTGATACTTTGATATCTTGt                                                                                          <  1:533313/141‑1 (MQ=11)

                                 aCTTGCTAGCATGATGCAAAAACGGCGGCAGGGCGAAAATACGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACATTGt                                                                                          >  1:813886/1‑141 (MQ=11)

                                  cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAACTGAAATGACCTGCGGTTCTTTTTATATAGAGCGTACATACACGCAATACCTTTTAAAGGATTCGTTGCTGTATTGATACTTTGATGCCTTGtc                                                                                         >  1:640348/1‑141 (MQ=11)

                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                                                        <  1:318619/141‑1 (MQ=11)

                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                                                        <  2:960581/141‑1 (MQ=11)

                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                        <  2:933216/141‑1 (MQ=17)

                                    tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                                                       >  1:164299/1‑141 (MQ=255)

                                     gCTAGCATGATGCTCATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                      >  2:750550/1‑141 (MQ=11)

                                     gCTAGCATGATGCTAATTTCATGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                      <  1:96742/141‑1 (MQ=11)

                                      cTAGGATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                     <  2:474538/141‑1 (MQ=17)

                                      cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                     >  2:788461/1‑141 (MQ=17)

                                       tAGCATTATGCTCATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                    <  1:118127/141‑1 (MQ=11)

                                       tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                    >  1:845793/1‑141 (MQ=32)

                                       tAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAGTACCTTTTAAAGTATTCTTCGCTGTATTGATACTTTGATACCTTGtctttct                                                                                    >  2:21884/1‑141 (MQ=12)

                                         gCATGATGCTAATTTAGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                  >  1:553575/1‑141 (MQ=17)

                                             gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                              <  2:576082/141‑1 (MQ=25)

                                                gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                                                                           >  1:996715/1‑141 (MQ=255)

                                                  tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAGTTTGATACATTGTCTATCTTACTTTAatat                                                                         >  1:210564/1‑141 (MQ=11)

                                                  tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                         >  2:197428/1‑141 (MQ=25)

                                                   aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                        >  2:452046/1‑141 (MQ=25)

                                                   aaTTTCGTGCGATGGCGAAAACCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                        <  1:45334/141‑1 (MQ=255)

                                                       tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTGTTTCTTACTTTAATATGACgg                                                                    >  2:511151/1‑141 (MQ=11)

                                                       tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACATCAAGATGAAGTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGGCgg                                                                    >  1:254439/1‑141 (MQ=11)

                                                        cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACggg                                                                   >  1:511906/1‑140 (MQ=255)

                                                        cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                   <  1:433659/141‑1 (MQ=255)

                                                         gTGGCATGGCGAAACTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGtt                                                                  >  2:376450/1‑140 (MQ=255)

                                                          tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTgt                                                                 >  1:1009902/1‑140 (MQ=255)

                                                          ggggaTGGGGGAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATACAGAGCGTAAATACATTCAGTACATTTAAAAGTATTCTTTGCTGTATTGATACTTTGATGGCTAGTCTTTCTTACTTTACTATGACTGTGa                                                                 <  2:438877/137‑1 (MQ=11)

                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAAATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                >  1:343533/1‑141 (MQ=255)

                                                             cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTAAATATGACGGTGATCt                                                              <  1:568823/141‑1 (MQ=38)

                                                               tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                            <  2:773669/141‑1 (MQ=255)

                                                                ggggAAAATCCGTAGATCTGAAGAGACCTCCGGTTCTTTTTATACAGAGCGTAAATACATTCAATACCTTTTAAAGTATCCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACAGGGCTCTTGc                                                           >  2:1093745/4‑141 (MQ=11)

                                                                ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  1:138386/138‑1 (MQ=14)

                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  2:419980/141‑1 (MQ=255)

                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  1:900097/141‑1 (MQ=255)

                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  1:409312/141‑1 (MQ=255)

                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAATGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                           <  2:814788/141‑1 (MQ=255)

                                                                  cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                         <  2:608328/141‑1 (MQ=255)

                                                                    aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                       >  2:493157/1‑141 (MQ=37)

                                                                      aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                     <  1:829626/141‑1 (MQ=255)

                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                 <  1:806727/141‑1 (MQ=255)

                                                                           gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTGAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTCTCTTTCTTACTTTAATATGACGATGATCTTCCTCTATAAGGtt                                                >  2:832418/1‑141 (MQ=11)

                                                                              gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                             <  1:1025909/141‑1 (MQ=255)

                                                                               aTCTGAAGAGTCCTTCGGTTCTTTTTATATAGAGCGTAAATACATTCAGTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATTTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                            <  2:885823/141‑1 (MQ=11)

                                                                                 ttGAAGGGACCTGCGGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                          <  2:259684/140‑1 (MQ=255)

                                                                                 cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                          <  1:955431/141‑1 (MQ=255)

                                                                                   gAAGCGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                        <  1:681460/141‑1 (MQ=255)

                                                                                      gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                     >  1:707672/1‑141 (MQ=38)

                                                                                      gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                     <  1:878406/141‑1 (MQ=38)

                                                                                        gaCCTGCCGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                   <  2:328294/141‑1 (MQ=255)

                                                                                          cctacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                 >  1:397377/5‑141 (MQ=255)

                                                                                            tGCTGTTTTGTTTATATGGTGCGTCAATACATTCAATNCCTTTTAAAGTATTCTTGTCTGTATTGATACTTTCATACCTTGTCTTTCTTACTTTATGATGACGGTGATGTTGCTCAATGAGGTTATTCAGATATTTCGATg                               <  2:10511/141‑1 (MQ=11)

                                                                                             acGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                              >  2:1070583/2‑141 (MQ=255)

                                                                                              cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAGGTTATTCAGATGTTTCGATTTa                             >  1:362533/1‑141 (MQ=255)

                                                                                                gTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGATGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                           >  2:862602/1‑141 (MQ=255)

                                                                                                  tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                         >  1:420320/1‑141 (MQ=255)

                                                                                                  tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                         <  1:592088/141‑1 (MQ=255)

                                                                                                  tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                         >  2:118536/1‑141 (MQ=255)

                                                                                                   cTTTTTATATAGAGCGTCAATACACTCAATCCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGCTCTTGCTCACTGCGCTTCTTCCGCTACTTCgcagcacaacg                        >  1:764036/1‑131 (MQ=11)

                                                                                                   cTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                        <  1:1051591/141‑1 (MQ=255)

                                                                                                     ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                      <  1:217846/141‑1 (MQ=255)

                                                                                                      tttATATAGAGCGTAACTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATAGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCAATGTACACTggcg                     >  2:6574/1‑138 (MQ=11)

                                                                                                      tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAGGAGGTTATTCAGATATTTCGATGTACAATggca                     <  1:486081/141‑4 (MQ=255)

                                                                                                       ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAAATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATCCAGATATTTCGATGTACAATGCCAg                    >  2:310404/1‑141 (MQ=14)

                                                                                                        tatataGAGCGTAACTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGTTCAATGAAGTTATTCAGATATTTCGATGTACAATGTCAGt                   <  1:791011/141‑1 (MQ=14)

                                                                                                        tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                   <  2:173047/141‑1 (MQ=255)

                                                                                                        tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCatt                   <  2:290504/141‑3 (MQ=255)

                                                                                                         atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                  >  1:1001815/1‑141 (MQ=255)

                                                                                                           ataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATGCATATATTTCGATGTACAATGACAGTCAg                <  1:1095973/141‑1 (MQ=14)

                                                                                                            taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTTCATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg               <  1:27072/141‑1 (MQ=255)

                                                                                                              gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt             >  2:349254/1‑141 (MQ=255)

                                                                                                                gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCAGgttta           <  1:657843/141‑1 (MQ=255)

                                                                                                                  gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag         <  1:622575/141‑1 (MQ=255)

                                                                                                                  gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag         <  2:406257/141‑1 (MQ=255)

                                                                                                                    aaaTTCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt       <  2:681194/141‑1 (MQ=255)

                                                                                                                    aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATAGATCCTTTGATAACTTGCATTTGTTACTTTAACATGTTGCTGTTCTTGCTCAATGAGTATATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt       >  2:921568/1‑141 (MQ=14)

                                                                                                                     aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt      <  2:989545/141‑1 (MQ=255)

                                                                                                                      aTACACTCAATACCTTTTAAAGTCTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta     >  1:373396/1‑141 (MQ=255)

                                                                                                                      aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta     >  2:523255/1‑141 (MQ=255)

                                                                                                                       tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa    >  2:187648/1‑141 (MQ=255)

                                                                                                                       tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa    >  1:233903/1‑141 (MQ=255)

                                                                                                                       tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGAGATTTCGATGTACAATGGCAGTCAGgtttaagtttaa    >  1:612592/1‑141 (MQ=255)

                                                                                                                       tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTACTCAATGAGGTTATTCAGATATTTCGATGTACATTGGCAGTCAGgtttaagtttaa    >  1:725169/1‑141 (MQ=255)

                                                                                                                       tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATATTGCTAAATGAGGTTATTCAGATATTTAGATGTACAATGGCAGTCAGatttaaatttaa    >  2:910554/1‑141 (MQ=14)

                                                                                                                       tACACTCAATACCTTTTAAAGTATTCTTCGCTGTATTGATACTTTGATACCTTGCCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGggttaagtttaa    >  2:59210/1‑141 (MQ=14)

                                                                                                                         cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTACGTTTacaa  >  1:2941/1‑141 (MQ=255)

TAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAA  >  CP000730/35847‑36108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`