Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,827,998 T→C 5.0% S14P (TCA→CCA)  icaD → intercellular adhesion protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,827,9980TC 5.0% 347.9 / 4.2 124S14P (TCA→CCA) icaDintercellular adhesion protein D
Reads supporting (aligned to +/- strand):  ref base T (50/65);  new base C (3/4);  total (54/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TTTGTAAGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGTTGC  >  CP000730/2827858‑2828137
                                                                                                                                            |                                                                                                                                           
tttGTAAGTTGGTATCCGACAGTATACTGGATCATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAATGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAat                                                                                                                                             <  1:737655/141‑1 (MQ=255)
  tGTAAGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAatca                                                                                                                                           <  1:381816/141‑1 (MQ=255)
      aGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGc                                                                                                                                       >  2:135218/1‑141 (MQ=255)
      aGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGc                                                                                                                                       >  1:266123/1‑141 (MQ=255)
        ttGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTa                                                                                                                                     <  1:51413/141‑1 (MQ=255)
            tATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAata                                                                                                                                 >  2:1053025/1‑141 (MQ=255)
               ccGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTg                                                                                                                              >  1:335770/1‑141 (MQ=255)
               ccGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGAAACATGGTCCAGCCCAGACCGCGGGAATACCCAACGCTAAAATCATCGCTAAatatgg                                                                                                                              >  2:732823/1‑139 (MQ=255)
                  aCAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTaa                                                                                                                           >  1:148353/1‑141 (MQ=255)
                     gTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAagag                                                                                                                        >  1:439819/1‑141 (MQ=255)
                      tataCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAgaga                                                                                                                       <  2:1104752/141‑1 (MQ=255)
                       ataCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGaa                                                                                                                      >  2:1101748/1‑141 (MQ=255)
                       ataCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATAGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAATGATTGTAagcaaa                                                                                                                      >  2:664829/1‑137 (MQ=255)
                       ataCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAAAGTAAGCAAGGTGGTTACGCAACATGGCCCAGCCCAGACAGAGGGAATACCCCACGCTAAAATCATCGCTAACTACTGTAggagca                                                                                                                      >  1:771440/1‑139 (MQ=255)
                       ataCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCAAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGaa                                                                                                                      >  1:756288/1‑141 (MQ=255)
                        taCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGaaa                                                                                                                     <  1:856101/141‑1 (MQ=255)
                         aCTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAAc                                                                                                                    <  1:784314/141‑1 (MQ=255)
                          cTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACa                                                                                                                   <  1:609241/141‑1 (MQ=255)
                            ggATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGc                                                                                                                 <  2:1035131/141‑1 (MQ=255)
                             gATTATTGTCGCAGCAGTTGTTCTTGTCGCATTTCAACAAGGCTTCCCGGGTCAGTTGAATGCTTACTGAAAATGGTCAAGCCTGGATTTGGGTAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCa                                                                                                                <  2:502550/141‑1 (MQ=255)
                             gATTATTAACGCAGCAGTAGTTCTTGTCGCCTTTCCAAAAGCATTCACACAAAATGTATGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCa                                                                                                                <  1:306346/141‑1 (MQ=255)
                             gATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCa                                                                                                                <  1:716687/141‑1 (MQ=255)
                               ttattaACGCCGCAGTCGTTGTTGTCGCATGTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACt                                                                                                              <  2:758736/141‑1 (MQ=255)
                                tattaACGCAGCAGTAGTTCTTGTCGCATTTCAAAAAGCATTAAACCGTAAGAAAGGTGGATACGCAACATGGTCAAGCCCCGACAGAGGGAATACCCAACGCTAAAATCATCGATAAATATTGTAAGAGAAACACCACtt                                                                                                             >  1:315898/1‑141 (MQ=255)
                                  ttaACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTAt                                                                                                           >  2:827703/1‑141 (MQ=255)
                                    aaCGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCACAAACAGACGGAAAACACAACGCAAAAATAATCGCTAAAAATTATAAGAGAAACACAACTTACCg                                                                                                         >  1:498818/1‑141 (MQ=255)
                                       gcagcaGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCta                                                                                                      >  2:282998/1‑141 (MQ=255)
                                             gtagtTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCCAAAAACATCGCTAAATATAGTACAAGAAACAGCACATATCGCtaacactt                                                                                                >  1:498901/1‑135 (MQ=255)
                                               agtTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCCGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATGGTAATAGAAAAAGCACTTATCGGTATAACAtgt                                                                                              >  2:110660/1‑141 (MQ=255)
                                                gtTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTtgtg                                                                                             >  1:353680/1‑141 (MQ=255)
                                                  tCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCt                                                                                          <  1:739355/141‑1 (MQ=255)
                                                  gcTTGGCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGGGGTTACGCAACATGGTCAAGCTCAGACAGAGGGAATACCCAACGCTGAAATCATCGCTAAATATTGTAAGAGCAACAGCATTTATCGCTATATCTTGTGTc                                                                                           <  1:714070/140‑1 (MQ=255)
                                                   tttGGCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCt                                                                                          <  2:325932/140‑1 (MQ=255)
                                                         gCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATCTCTTGTGTCTTTTGGa                                                                                    >  2:395371/1‑141 (MQ=255)
                                                           aTTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGata                                                                                  <  2:154492/141‑1 (MQ=255)
                                                             ttCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGAAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGatata                                                                                <  1:613671/141‑1 (MQ=255)
                                                               ccAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTTAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATAtt                                                                              <  2:16709/141‑1 (MQ=255)
                                                                cAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTg                                                                             <  2:410398/141‑1 (MQ=255)
                                                                        ttAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttg                                                                     >  2:930758/1‑141 (MQ=255)
                                                                        atAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttg                                                                     <  2:616320/140‑1 (MQ=255)
                                                                         tAAAACGGAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttgt                                                                    >  1:694403/1‑141 (MQ=255)
                                                                          aaaaCGTAAGAAAGGTGGTTACGCCACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttgtt                                                                   <  1:252189/141‑1 (MQ=255)
                                                                          aaaaCGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttgtt                                                                   <  2:956169/141‑1 (MQ=255)
                                                                          aaaaCGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttgtt                                                                   <  1:377176/141‑1 (MQ=255)
                                                                          aaaaCGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAgttgtt                                                                   <  1:220148/141‑1 (MQ=255)
                                                                           aaaGGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACTCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTc                                                                  <  2:727141/141‑1 (MQ=255)
                                                                           aaaCGTAAGAAAGGTGGTCACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTc                                                                  <  1:354042/141‑1 (MQ=255)
                                                                                 aaaaaaGGTTGTTACGCAACAAGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCg                                                            <  2:223529/138‑1 (MQ=255)
                                                                                 aaGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCg                                                            >  2:544031/1‑141 (MQ=255)
                                                                                 aaGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCg                                                            >  2:544063/1‑141 (MQ=255)
                                                                                 aaGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCg                                                            >  2:235355/1‑141 (MQ=255)
                                                                                  aGAAAGGTGGTTACGCAACATGGTGAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGt                                                           >  1:706835/1‑141 (MQ=255)
                                                                                  aGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGt                                                           <  1:839750/141‑1 (MQ=255)
                                                                                  aGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGt                                                           <  2:475146/141‑1 (MQ=255)
                                                                                    aaaGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGttt                                                         <  1:370989/141‑1 (MQ=255)
                                                                                    aaaGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGATATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGttt                                                         <  2:709525/141‑1 (MQ=255)
                                                                                     aaGGTGGTTACGTAACTTGGTAGAGGCCAGACAGAGGGAATACCTCACGTTAAAATCATCAATTATTCTTGTATTTAAAACCGAACTTTCCAGTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTta                                                        <  1:1092333/141‑1 (MQ=255)
                                                                                     aaGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTta                                                        <  2:439819/141‑1 (MQ=255)
                                                                                      aGGTCGTTACGCCACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAATATCATCGCTAAATATTGTAAGAGAAACATCACTTATCGCTATAGCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTtat                                                       <  2:215576/141‑1 (MQ=255)
                                                                                        gtggtTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTatat                                                     >  1:674199/1‑141 (MQ=255)
                                                                                         tggtTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTATTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATAtt                                                    >  2:1002668/1‑141 (MQ=255)
                                                                                          ggtTACGCAACATGGTCAAGCCCCGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTg                                                   <  1:955256/141‑1 (MQ=255)
                                                                                          ggtTACGCAACATGGTCAAGCCCAGATAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTGATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTg                                                   <  1:675849/141‑1 (MQ=255)
                                                                                          ggtTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTg                                                   <  2:51976/141‑1 (MQ=255)
                                                                                          ggtTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTg                                                   <  1:102594/141‑1 (MQ=255)
                                                                                          gggtACGCAACATGGTCACGCCCAGAAATAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGACACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTg                                                   <  2:735238/138‑1 (MQ=255)
                                                                                           gtTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGCTTCCTTGTTCTACTCGTTAATAGTgt                                                  >  2:758457/1‑140 (MQ=255)
                                                                                                  aaCATGGTCAAGCCCAGACAGAGTGAAGACCCAACGCTAGAATCATCGCTAAATATTGTAAGAGAAACGGCACTTATCGCTATATCTCGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACtata                                           <  2:157918/141‑1 (MQ=255)
                                                                                                  aaCATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACtata                                           <  2:552605/141‑1 (MQ=255)
                                                                                                  aaCATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACtata                                           <  2:269802/141‑1 (MQ=255)
                                                                                                   aCATGGTCAAGCCCAGACCGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTCTATATTGGTACTatat                                          <  1:654865/141‑1 (MQ=255)
                                                                                                    cATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATAGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATAtt                                         <  1:144844/141‑1 (MQ=255)
                                                                                                     aTGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATAttt                                        >  2:131649/1‑141 (MQ=255)
                                                                                                     aTGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATAttt                                        >  2:602996/1‑141 (MQ=255)
                                                                                                       ggTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGa                                      >  1:687425/1‑141 (MQ=255)
                                                                                                        gTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTCaa                                     >  1:59560/1‑141 (MQ=255)
                                                                                                             gCCTAGACAGAGGAAATACCCAACCCTAAAACCATCGCTAAAAATTGTAAGAGAAACCGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCa                                <  1:105592/141‑1 (MQ=255)
                                                                                                             gCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCa                                <  1:105541/141‑1 (MQ=255)
                                                                                                               ccAGAGAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTTTCTCCGTACTATATTTGAAATTCATg                              <  2:508195/141‑1 (MQ=255)
                                                                                                                   acagaGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGa                          <  2:919478/141‑1 (MQ=255)
                                                                                                                    cagaGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGaa                         >  2:1023086/1‑141 (MQ=255)
                                                                                                                    cagaGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGaa                         <  1:1011130/141‑1 (MQ=255)
                                                                                                                    cagaGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGGTGTTCTACTCGTTTTTATCTGTACTATATTTGAAAGTCATGACGaa                         <  2:187055/141‑1 (MQ=255)
                                                                                                                    cagaGGGAATACCCAACGCTAAAATCATCGCTAAATATTGGAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGaa                         <  1:281859/141‑1 (MQ=255)
                                                                                                                        ggtaaTACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATACTGGTACGATATTTGAAATTCATGACGAAAGTa                     <  1:533710/138‑1 (MQ=255)
                                                                                                                              aCCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTTGTACTTTATTTGATAATCATGACGAAAGTGTcaata               >  2:1000373/1‑141 (MQ=255)
                                                                                                                              aCCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATcaata               >  2:1050356/1‑141 (MQ=255)
                                                                                                                                ccAACGCTAAAATCATCGCTAAATATAGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTGATATTGGTACTATATTTGAAATTCATGACGAAAGTATcaataca             <  1:672960/141‑1 (MQ=255)
                                                                                                                                  aaCGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATcaatacaat           >  2:564954/1‑141 (MQ=255)
                                                                                                                                   aCGCTAAATTCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATcaatacaata          <  1:394101/141‑1 (MQ=255)
                                                                                                                                   aCGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATcaatacaata          <  2:697690/141‑1 (MQ=255)
                                                                                                                                     gCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACg        <  1:544031/141‑1 (MQ=255)
                                                                                                                                     gCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACg        <  1:544063/141‑1 (MQ=255)
                                                                                                                                     gCTAAAACCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACg        <  1:235355/141‑1 (MQ=255)
                                                                                                                                       tAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACgtg      <  1:827703/141‑1 (MQ=255)
                                                                                                                                         aaaTCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGtt    >  1:217271/1‑141 (MQ=255)
                                                                                                                                         aaaTCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGtt    >  2:177597/1‑141 (MQ=255)
                                                                                                                                           atcatcGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTCTCAAATCAATCCGTTTTgt  >  2:558858/1‑140 (MQ=255)
                                                                                                                                           atcatcGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGTTGc  >  2:318062/1‑141 (MQ=255)
                                                                                                                                           atcatcGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTGGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGTTGc  <  1:25194/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTTGTAAGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAAAATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCTTGTGTCTTTTGGATATATTGTTTAGTTGTTCTACTCGTTTATATTGGTACTATATTTGAAATTCATGACGAAAGTATCAATACAATACGTGTTGC  >  CP000730/2827858‑2828137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: