Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,519,798 T→A 3.5% S278S (TCA→TCT narG ← nitrate reductase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,519,7980TA 3.5% 533.8 / 5.2 170S278S (TCA→TCTnarGnitrate reductase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (74/90);  new base A (3/3);  total (77/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 173 total aligned reads displayed.

AAGCGTTCATTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCT  >  CP000730/2519658‑2519937
                                                                                                                                            |                                                                                                                                            
aaGCGTTCATTTGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACt                                                                                                                                              <  1:317653/141‑1 (MQ=255)
aaGCGTTCATTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACt                                                                                                                                              >  2:352866/1‑141 (MQ=255)
  gCGTTCATTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGa                                                                                                                                            >  1:894755/1‑141 (MQ=255)
     ttCATTAGGTTGATTAACATACTGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAAt                                                                                                                                         <  2:107822/141‑1 (MQ=255)
     ttCATTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCGTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAAt                                                                                                                                         <  2:154165/141‑1 (MQ=255)
       cATTAGGTTGATTAACATAATGTTCTTGTAACATAACATGCGTCATTGCTTGTGCACTTGCAGCATCTGAACCAGGATTCGGTGCTAGCAAGTTATCTGCAAATTTCACATTTCCTGCGTACTCTGGTGCTACTGAAATGa                                                                                                                                       <  2:924987/141‑1 (MQ=255)
        aTTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGGATCTGAACCAGGATTCGGTGCTAGCTAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACAGAAATGAc                                                                                                                                      >  1:793780/1‑141 (MQ=255)
        aTTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGAc                                                                                                                                      >  1:823885/1‑141 (MQ=255)
        aTTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACAGAAATGAc                                                                                                                                      >  1:123417/1‑141 (MQ=255)
        aTTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTAATGAAATGAc                                                                                                                                      <  1:810550/141‑1 (MQ=255)
        aTTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTAGAGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACAGAAATGAc                                                                                                                                      >  1:984162/1‑141 (MQ=255)
        aTTAGGTTGATCAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGAc                                                                                                                                      <  1:925941/141‑1 (MQ=255)
         ttAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACt                                                                                                                                     <  2:980278/141‑1 (MQ=255)
         ttAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACt                                                                                                                                     <  1:339646/141‑1 (MQ=255)
         ttAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACt                                                                                                                                     <  1:27048/141‑1 (MQ=255)
          tcggTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACtt                                                                                                                                    <  2:300770/139‑1 (MQ=255)
          tAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAGTGCCtt                                                                                                                                    >  1:343878/1‑141 (MQ=255)
            ggTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGCACAAGGTTGCTGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACtttt                                                                                                                                  <  1:529262/141‑1 (MQ=255)
            ggTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACtttt                                                                                                                                  <  1:705846/141‑1 (MQ=255)
                  ttAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCt                                                                                                                            >  2:855069/1‑141 (MQ=255)
                  ttAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTAGtccat                                                                                                                            >  2:158395/1‑137 (MQ=255)
                   tAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCtt                                                                                                                           >  1:430152/1‑141 (MQ=255)
                     aCATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGCAATGACTTTTGTACCTTta                                                                                                                         >  2:634629/1‑141 (MQ=255)
                     aCATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTACAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCtttt                                                                                                                         >  1:176369/1‑140 (MQ=255)
                      cATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTATTGAAATGACTTTTGTTCGtttgt                                                                                                                        >  2:218790/1‑139 (MQ=255)
                      cATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGCAATGACTTTTCTACATTtat                                                                                                                        >  2:710521/1‑141 (MQ=255)
                      cATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTtat                                                                                                                        >  2:379539/1‑141 (MQ=255)
                         actGTTCTTGTAAAATAACATAAGTCATTGCTTGTGCAATTGCAGCATCTGAACAAGGATTCGGTGCTAGCCAGTTCTCTGCAAATTTAACATTTTCTGCGTAATCTGGTGCTACTGAAATGAGTTTTGTACCttaagaga                                                                                                                     >  2:938863/3‑135 (MQ=255)
                           tGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCgg                                                                                                                   <  1:162874/141‑1 (MQ=255)
                             ttcctctAAAACAACAAGCCCCATTGCTTCTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATCTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGCAc                                                                                                                 <  2:896629/135‑1 (MQ=255)
                             ttCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCTTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGAc                                                                                                                 <  2:588012/141‑1 (MQ=255)
                               cTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCgggttt                                                                                                               <  2:557705/141‑5 (MQ=255)
                                 tGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCa                                                                                                             <  2:331847/141‑1 (MQ=255)
                                  gTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAg                                                                                                            <  1:958321/141‑1 (MQ=255)
                                  gTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAg                                                                                                            >  1:669233/1‑141 (MQ=255)
                                    taaaTAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTc                                                                                                          <  2:368078/140‑1 (MQ=255)
                                    aaaaTAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTc                                                                                                          <  2:1098900/141‑1 (MQ=255)
                                    aaaaTAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTc                                                                                                          <  2:781385/141‑1 (MQ=255)
                                     aaaTAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTCTAGCGGACTTCAGTCa                                                                                                         >  2:689325/1‑141 (MQ=255)
                                     aaaTAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCGACTGAAATGACTTTTGTACCTTTATGACGGCCTTCAggaa                                                                                                         >  1:287690/1‑138 (MQ=255)
                                           cATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAAt                                                                                                   <  2:669233/141‑1 (MQ=255)
                                            aTGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAAtg                                                                                                  <  2:904132/141‑1 (MQ=255)
                                             tGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGGCTTTTGTACCTTTATAGCGGACTTCAGTCATAAAAtgt                                                                                                 <  2:523938/141‑1 (MQ=255)
                                              gCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAAtgtg                                                                                                >  1:66882/1‑141 (MQ=255)
                                              gCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTAAtgatatttg                                                                                                >  2:224486/1‑133 (MQ=255)
                                                     tGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCgg                                                                                         <  1:988036/141‑1 (MQ=255)
                                                     tGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCgg                                                                                         <  2:327827/141‑1 (MQ=255)
                                                     tGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCgg                                                                                         >  1:96144/1‑141 (MQ=255)
                                                      gCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGa                                                                                        >  1:201764/1‑141 (MQ=255)
                                                            gCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGtcagg                                                                                  >  1:903724/1‑137 (MQ=255)
                                                             cAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACgtg                                                                                 <  2:96144/141‑1 (MQ=255)
                                                               aTTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTCCGTGtt                                                                               >  2:371968/1‑141 (MQ=255)
                                                                 tGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTaa                                                                             <  1:1008510/141‑1 (MQ=255)
                                                                 tGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTaa                                                                             >  2:472646/1‑141 (MQ=255)
                                                                 tGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTaa                                                                             >  1:950852/1‑141 (MQ=255)
                                                                   cagcaTCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGGGTTAAAg                                                                           >  2:26538/1‑141 (MQ=255)
                                                                    agcaTCTGAACCAGGATTAGGTGCTAGAAAGTTATTTGGAAATTTCACATTTTCTGTGGAATCTGGTGCTACTGAAATGACTTTTGTAACTTTATAGCGGAATTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAgg                                                                          >  2:397921/1‑141 (MQ=255)
                                                                    agcaTCTGAAACAGGCATCGGTACTAGCCGGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTATACCTTTATAGCGGACTTCGGTCATAAAATGTGCATCCGGAGTACGTGTTAAAga                                                                          >  2:952292/1‑140 (MQ=255)
                                                                     gcaTCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGCGTACGTGTTAAAGGt                                                                         <  1:552834/141‑1 (MQ=255)
                                                                        tCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGCCTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAACGGtccc                                                                      >  2:541424/1‑138 (MQ=255)
                                                                        tCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGGTAAAGGTACa                                                                      >  1:446022/1‑141 (MQ=255)
                                                                          tGAACCCGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACAtt                                                                    <  1:63110/141‑1 (MQ=255)
                                                                          tGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACAGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACAtt                                                                    <  2:123417/141‑1 (MQ=255)
                                                                          tGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGAGCTACAGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACAtt                                                                    <  2:793780/141‑1 (MQ=255)
                                                                          ggAACCAGGATGCGGTGCTAGTCAGTTAGCGGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACAtt                                                                    <  1:296517/140‑1 (MQ=255)
                                                                           gAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAGGGTACATTa                                                                   <  1:811948/141‑1 (MQ=255)
                                                                           gAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTa                                                                   <  2:658022/141‑1 (MQ=255)
                                                                            aaCCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTag                                                                  >  2:293874/1‑141 (MQ=255)
                                                                            aaCCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGCGTTAAAGGTACATTag                                                                  <  1:550665/141‑1 (MQ=255)
                                                                              ccAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGGTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTagag                                                                >  1:861487/1‑141 (MQ=255)
                                                                                  gATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGcccc                                                            <  2:430152/141‑1 (MQ=255)
                                                                                     tCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCcaca                                                         <  1:891984/141‑1 (MQ=255)
                                                                                     tCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCcaca                                                         <  1:372513/141‑1 (MQ=255)
                                                                                           cTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTTTTTTTTTATTGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGCGCCCCACAtaataa                                                   >  2:415335/1‑141 (MQ=255)
                                                                                            tAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATaataat                                                  <  2:18589/141‑1 (MQ=255)
                                                                                             aGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATg                                                 >  1:726519/1‑141 (MQ=255)
                                                                                             aGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATg                                                 >  1:257451/1‑141 (MQ=255)
                                                                                                cAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTat                                             >  2:103386/1‑141 (MQ=255)
                                                                                                  gTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTatga                                           <  2:1057171/141‑1 (MQ=255)
                                                                                                   ttATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTatgat                                          >  2:168547/1‑141 (MQ=255)
                                                                                                   ttATCTCCAAATTCCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTatgat                                          <  1:367252/141‑1 (MQ=255)
                                                                                                     aTCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAAGGTatgatgg                                        >  2:1089381/1‑140 (MQ=255)
                                                                                                       cTGCAAGTTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCAGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGt                                      >  2:694763/1‑141 (MQ=255)
                                                                                                       cTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGCGTACGGGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGt                                      >  1:748586/1‑141 (MQ=255)
                                                                                                            aaTTTCACCTTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATAc                                 <  1:233286/141‑1 (MQ=255)
                                                                                                            aaTTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATAc                                 <  1:740913/141‑1 (MQ=255)
                                                                                                            aaTTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATAc                                 <  2:513418/141‑1 (MQ=255)
                                                                                                             aTTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATAcc                                >  1:4869/1‑141 (MQ=255)
                                                                                                                   caTTTTCTGCGTAATCTGGTGCGACTGAAATGACTTTTGTACCTTTATCGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCAc                          <  1:224367/141‑1 (MQ=255)
                                                                                                                    cttgtcagcttcggcTGGTGCTGCTGAACTTACTTTTGTCGCTTTATAGCTGACTTCAGTCATAAAATGTGCATCCGGAGTACGTTTTACAGTTACATTAGAGCCCCACCCAAAATGTTTTGATGCGTTATACCAGTCACt                         <  2:783617/127‑1 (MQ=255)
                                                                                                                     ttttCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACtt                        <  1:168547/141‑1 (MQ=255)
                                                                                                                     ttatCTGCGTAACCGGGTGCTACTGAAATGACTTTTGTACCTTTAGAGCGGACTTCAGTCAGAAAATGTGCATCCGGAGTACGTGTTAAAGGTGCATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACtt                        <  2:716121/138‑1 (MQ=255)
                                                                                                                      tttCTGCTGAATCTTGTGTCACTGAAATGACTTTTGTCCCTTTATAGCGGACTTCAGTCATAAACTGTGCATTCGGAGGACGTCTTAAAGGTACATTAGAGCCCCACAGAATAATGTATGATGCGTTATACCAGTCACTTg                       <  2:147710/141‑1 (MQ=255)
                                                                                                                         cTGCGTAATCTGGTGCTACTGAAATGGCTTTTGTACCTTTATAGCGGACTTCAGTGATAAATTGTGCATCCGGAGCACGTGTTACTGGTACAGGAGAGCCCCACATAATAATGTATGATGCGTCATACCAGTCACTTGAtc                    >  1:1037610/1‑140 (MQ=255)
                                                                                                                         cTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATACGGTATGATGCGTTATCACAGTCACTTGCtt                    >  2:900453/1‑141 (MQ=255)
                                                                                                                         cTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGGCACTTTAtt                    >  2:585426/1‑141 (MQ=255)
                                                                                                                                     gttcTACTGAAATGACTTTTGTACCTTTATAGCGCACTTCATTCATAAAAGGTGCAGCCGGAGTACGTGTTAACGGTACCTTAACACCCACCCTTATCTTGGAGGATGCGTTATACCAGTCTCTTGATTCAGGCACATCTg        <  2:124660/138‑1 (MQ=255)
                                                                                                                                        tatatGAACTTACTTTTGCACCTTTATAGCGGACTTCAGTCATAAAATGTCCATTCGGAGTACGTGTTAAAGGTATATTAGCGCCCAGCCATCTAATCTAGCCAAAGTTATACCAGTCACTTGATTCAGGCACATCTGttt     <  2:671767/137‑1 (MQ=255)
                                                                                                                                          aCTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGc   >  1:75258/1‑141 (MQ=255)
                                                                                                                                           cTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGct  >  2:654099/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAGCGTTCATTAGGTTGATTAACATAATGTTCTTGTAAAATAACATGCGTCATTGCTTGTGCAATTGCAGCATCTGAACCAGGATTCGGTGCTAGCCAGTTATCTGCAAATTTCACATTTTCTGCGTAATCTGGTGCTACTGAAATGACTTTTGTACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCT  >  CP000730/2519658‑2519937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: