Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,464,772 A→T 4.6% L344M (TTG→ATG)  USA300HOU_2327 ← isopentenyl‑diphosphate delta‑isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,464,7720AT 4.6% 379.5 / 7.5 132L344M (TTG→ATG) USA300HOU_2327isopentenyl‑diphosphate delta‑isomerase
Reads supporting (aligned to +/- strand):  ref base A (68/57);  new base T (3/3);  total (72/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 135 total aligned reads displayed.

TATTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTCTACATAAGCTACTGTAT  >  CP000730/2464632‑2464912
                                                                                                                                            |                                                                                                                                            
tATTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCaa                                                                                                                                              >  1:952593/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGGGGGCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAg                                                                                                                                             >  1:990288/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAg                                                                                                                                             >  1:623290/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAg                                                                                                                                             >  1:756298/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAg                                                                                                                                             >  1:606476/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAg                                                                                                                                             >  1:410531/1‑141 (MQ=255)
 aTTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCAATGTATTTTCAAg                                                                                                                                             >  2:15128/1‑141 (MQ=255)
   ttttgGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGtt                                                                                                                                           <  2:288300/139‑1 (MQ=255)
   tgttgGTGCATTCTTAATCGATTTAATGGGATTTATAGTCATTATGGGATTCATACAAGGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGtt                                                                                                                                           <  2:1004559/141‑1 (MQ=255)
    gttgGTGCATTCTTAATCGATTTAATTGGTGTTATTGTCATTATGGGATTCATACAATGGTTTATTTAAACACCAAACTCATAAATAAAAGAGCAGGCCTTCGCCTCCTCTTTCATTTATCCTCGATGTATATTCAAGTTa                                                                                                                                          <  2:847180/141‑1 (MQ=255)
      tgGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACg                                                                                                                                        <  2:90542/141‑1 (MQ=255)
        gtgcacctttccccGATTTACCTCGTGTTACACTCCTTATGGGATTCCTACAACCGTTTAGTCAACCACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACgtt                                                                                                                                      <  1:228754/128‑1 (MQ=255)
            aTTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTc                                                                                                                                  <  1:887763/141‑1 (MQ=255)
             ttCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCt                                                                                                                                 <  2:943814/141‑1 (MQ=255)
               cTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTAGGTTGTTCTAt                                                                                                                               >  1:1045949/1‑141 (MQ=255)
               cTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTAt                                                                                                                               >  2:189864/1‑141 (MQ=255)
               cTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTAt                                                                                                                               >  1:125915/1‑141 (MQ=255)
               cTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTAt                                                                                                                               >  1:384080/1‑141 (MQ=255)
               cTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCATCGCCCCCACTTTCATCTACACCAGATGGAGATTCAAGTTACGTTGTTCTAt                                                                                                                               >  1:145761/1‑141 (MQ=255)
                   aTCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATACTCGATGTATATTCAAGTTACGTTGTTCTATccgt                                                                                                                           >  1:238109/1‑139 (MQ=255)
                    tCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTGTTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATACAtt                                                                                                                          >  2:205849/1‑140 (MQ=255)
                         ttAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAat                                                                                                                     <  2:803061/141‑1 (MQ=255)
                          ttatttatgtactaGTAAATATGGGATTCATACAATGGTTTAGTTAAACTCCAAACTCATAAATAAAAGAGGAGGCCCTCGCCTCCTCAGTTATTTATCCTCGATGTATATTCAAGTTACGCTGTTCTATCCATGataata                                                                                                                    <  2:189978/129‑1 (MQ=255)
                          tAATTGGTGTTATAGTCATTATGGGTTTCATACAAGGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAata                                                                                                                    <  1:334796/141‑1 (MQ=255)
                           aaTTGGTGTTATAGTCATTATGGGACTCATATAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTACCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAatat                                                                                                                   <  2:644133/141‑1 (MQ=255)
                                gagtcataGTCGTTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTGTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCg                                                                                                              <  1:240972/136‑1 (MQ=255)
                                 tgtTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATATATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCgg                                                                                                             >  1:717002/1‑141 (MQ=255)
                                 tgtTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTTTCCATGACAATTTTTCCgg                                                                                                             >  2:540363/1‑141 (MQ=255)
                                        gTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATCAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAAt                                                                                                      >  2:705029/1‑141 (MQ=255)
                                        gTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAAt                                                                                                      >  1:624239/1‑141 (MQ=255)
                                        gTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCACCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTaaaa                                                                                                      >  2:446436/1‑140 (MQ=255)
                                         tCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAAtc                                                                                                     >  2:174123/1‑140 (MQ=255)
                                         tCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATa                                                                                                     >  1:1049531/1‑141 (MQ=255)
                                            ttATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAGAAGAGGCGCCCTTCGCCTCCTCTTTTATTTATACTCGATGTATATTCAAGTTCCGTTGTTCTATCCATGACAATATTTCTGGACTAAATAcca                                                                                                  >  1:74125/1‑139 (MQ=255)
                                              atggacttCATACAATGGTTTACTTAAACATCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTTTTCTATCCATGACAATATTTCCGGACTAAATACGAtt                                                                                                <  2:207257/135‑1 (MQ=255)
                                                gggATTCATACAATGGTTTAGTTACACACCAAACTCATAAATAAAAGAGGAGGGCTTCGCCTCGTCTTTTATTGATCCTCGATGTATATTCAAGTTACGTTGTTCTATCTATGACAATATTTCCGGACTAAATACGATTTg                                                                                              >  1:808430/1‑141 (MQ=255)
                                                gggATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTg                                                                                              >  1:945324/1‑141 (MQ=255)
                                                gggATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTg                                                                                              >  2:882031/1‑141 (MQ=255)
                                                 ggATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCCTGACAATATTTCCGGACTAAATACGATTAGt                                                                                             >  2:428878/1‑141 (MQ=255)
                                                 ggATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTAGATGTATATTCAGGTTACGTTGTCCTATCCATGACAATATTTCCGGACTAAATACGATTTGt                                                                                             >  2:324694/1‑141 (MQ=255)
                                                   aTTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGttt                                                                                           <  1:685487/141‑1 (MQ=255)
                                                   aTTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCGCGATGTATATTCAATTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGttt                                                                                           <  1:528874/141‑1 (MQ=255)
                                                    ttCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGtttt                                                                                          >  2:794373/1‑141 (MQ=255)
                                                    ttCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGtttt                                                                                          >  2:796101/1‑141 (MQ=255)
                                                    ttCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGtttt                                                                                          >  2:665152/1‑141 (MQ=255)
                                                    ttCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGtttt                                                                                          >  2:811000/1‑141 (MQ=255)
                                                        tACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTtgtg                                                                                      >  1:561176/1‑141 (MQ=255)
                                                         acacTGGTTTAGTTAAACACCAAACTCATAAATCAAAGACGAGGCCTTCGCTTCCTCTTTTATTCATTCTCGATGTATCTTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGCTTTGTTTTTgggt                                                                                     >  2:867843/1‑141 (MQ=255)
                                                         aCAATGTTTTACTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTgtgt                                                                                     <  2:1076589/141‑1 (MQ=255)
                                                         aCAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTgtgt                                                                                     >  2:396665/1‑141 (MQ=255)
                                                         aCAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTgtgt                                                                                     <  2:240911/141‑1 (MQ=255)
                                                                  tAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAgtcgtc                                                                            >  1:709963/1‑141 (MQ=255)
                                                                    gTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCaa                                                                          <  1:688002/141‑1 (MQ=255)
                                                                    gTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCaa                                                                          >  1:59935/1‑141 (MQ=255)
                                                                    gTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCaa                                                                          <  1:352494/141‑1 (MQ=255)
                                                                    gTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTATTCTATCCATGACAATATTTCCAGACTAAATACGATTTGTATTTGTGTTACATCGTCaa                                                                          >  1:146871/1‑141 (MQ=255)
                                                                        accacCAAACGCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTGTATTTATACTCGATGTATATTCCATTTACGTTGTGCTATCCCTGACAATATTTCCGGACTACATACGATTTGTTTTTGTGTCAAGTCGTCAATAtt                                                                      >  2:525630/3‑141 (MQ=255)
                                                                        aaCAGCAAACTTATTAATAAAAGAGGAGGCCTTCGCCTAATCTTTTATTTATCCTACATGTATATTCAATTTCCGTTATTCCATCCATGAAAATATTTGCGGACTAAATACGTATTGTTTGTGTATTAAGTCGTCAACAtt                                                                      <  2:403286/141‑1 (MQ=255)
                                                                        aaCACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAATTACGATTTGTTTTTCTGTTTGTTCGTCAATAtt                                                                      >  1:941033/1‑141 (MQ=255)
                                                                        aaCACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATAtt                                                                      <  1:930404/141‑1 (MQ=255)
                                                                        aaCACCAAACTCATAAATAAAAGAGGAGGCATTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATAGGATTTGTTTTTGTGTTAAGGCGTCAATAtt                                                                      >  1:335312/1‑141 (MQ=255)
                                                                                        ataaaAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGGTTTGTTTTTGTGTTAGGTCGTCAATATTTTTAGCATCTacgagc                                                      >  2:29091/1‑136 (MQ=255)
                                                                                          aaaaGAGGAGTCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGt                                                    <  2:663717/141‑1 (MQ=255)
                                                                                          aaaaGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAAGACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGt                                                    <  1:437440/141‑1 (MQ=255)
                                                                                          aaaaGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCCATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAACATTTTTCGCATCTAACATCGt                                                    <  2:757726/141‑1 (MQ=255)
                                                                                          aaaaGAGGAAGCCCTCGCCTCCCTTTTTATTTATCCTCGATGTATATTCAAGTGACGTTGTTCTATCCATGACAACATTTCCGGACGAAATACGATTTGTTGTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGt                                                    <  2:1044816/141‑1 (MQ=255)
                                                                                              gaggagGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTGACATCGTCatt                                                >  2:724420/1‑141 (MQ=255)
                                                                                                ggaggcgaaggCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCattat                                              >  1:320689/11‑141 (MQ=255)
                                                                                                ggaggcgaaggCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGCCATTGAGTTGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCattat                                              >  1:1086998/11‑141 (MQ=255)
                                                                                                  aggcgaaggCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTg                                            >  1:16545/9‑141 (MQ=255)
                                                                                                  aggCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTg                                            <  1:882031/141‑1 (MQ=255)
                                                                                                   ggCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGa                                           <  1:89100/141‑1 (MQ=255)
                                                                                                    gcgaaggCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGAt                                          >  2:842420/7‑141 (MQ=255)
                                                                                                      cTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATCTATGCCGTCTAACATCGTCATTATTGAttt                                        <  2:236552/141‑1 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGttttt                                       >  1:52076/1‑140 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTc                                       >  1:184200/1‑141 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTc                                       <  1:1020323/141‑1 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTc                                       >  1:851578/1‑141 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTc                                       >  1:864468/1‑141 (MQ=255)
                                                                                                       ttCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTc                                       <  1:1080535/141‑1 (MQ=255)
                                                                                                        tCGCCTTCTCTTGTATTTATCCTCGATGTCTATTCAAGTTCCGTGGCTCTATCCATGACAATATTTCCGGACTAAATACGAGTTGTTTTTGTGTTAAGTCGTCAATATGTTTAGCATCTAACATCGTCATTATTGATTTCa                                      <  2:8906/141‑1 (MQ=255)
                                                                                                         cgtctcctcTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCAt                                     <  2:127308/138‑1 (MQ=255)
                                                                                                           cctcctCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCAtgt                                   <  1:542381/141‑1 (MQ=255)
                                                                                                           cctcctCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTAGTGTTAAGTCGTCAATATTTTTAGCCTCTAACATCGTCATTATTGATTTCAtgt                                   <  1:713788/141‑1 (MQ=255)
                                                                                                            ctcctcTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCAtgtg                                  <  2:72827/141‑1 (MQ=255)
                                                                                                               ctctTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTc                               >  1:647356/1‑141 (MQ=255)
                                                                                                                 ctTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCaa                             <  2:303965/141‑1 (MQ=255)
                                                                                                                   tttatttaTCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCTTCAATCTTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATa                           >  2:1092113/1‑141 (MQ=255)
                                                                                                                          atCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGAtt                    <  1:842420/141‑1 (MQ=255)
                                                                                                                           tacTCGATGTATATTCAAGTTACGTTTTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCCTCAACATTTTTAGCATCTAACATCGTCATTAATGATTTAATGAGTCCAAGAACTCATTc                   <  2:813311/139‑1 (MQ=255)
                                                                                                                           tCCTCGATGTATATTCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGTTTc                   <  1:794373/141‑1 (MQ=255)
                                                                                                                           tCCTCGATGTATATTCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTc                   <  1:796101/141‑1 (MQ=255)
                                                                                                                           tCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTc                   <  1:186165/141‑1 (MQ=255)
                                                                                                                           tCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTc                   <  1:1028373/141‑1 (MQ=255)
                                                                                                                           tCCTCGATGTATATGCATGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTc                   <  1:665152/141‑1 (MQ=255)
                                                                                                                                 aTGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTCTACATa             >  2:480374/1‑141 (MQ=255)
                                                                                                                                   gTATATTCAAGTTACGTTGTTCTATCCATGACACTATTTCCGGCCTAAATAAGATTTGTTTTTGTGTTAAGTCGTCAATATATTTAGCATCTAACATCGTCATTATTTATGTCATGTGTTCAATAAATGATTCTCCATGAg           >  2:31803/1‑141 (MQ=255)
                                                                                                                                      tcttCAATTTTCGTTTTTCTTTCCATTACAATATTTCCGACCTTAATACGATTTGTTTTTGTGCTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTCTACATAAGCTa        <  2:46107/139‑1 (MQ=255)
                                                                                                                                      tatTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTCTACATAAGCTa        <  1:450270/141‑1 (MQ=255)
                                                                                                                                            aGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAACGCTTCTGTATAACATACTGTAt  >  2:929984/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TATTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGGTTTAGTTAAACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCCTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTCTATCCATGACAATATTTCCGGACTAAATACGATTTGTTTTTGTGTTAAGTCGTCAATATTTTTAGCATCTAACATCGTCATTATTGATTTCATGTGTTCAATAAATGATTCTACATAAGCTACTGTAT  >  CP000730/2464632‑2464912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: