Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,227,320 T→G 23.1% I40L (ATT→CTT)  atpB ← F‑ATPase superfamily proton(H+)‑ or sodium (Na+)‑translocating F‑type ATPase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,227,3200TG23.1% 86.4 / 28.0 66I40L (ATT→CTT) atpBF‑ATPase superfamily proton(H+)‑ or sodium (Na+)‑translocating F‑type ATPase subunit A
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base G (7/8);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

CAGCGTTACTGCTAAGAAGTGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTAATTTA  >  CP000730/2227180‑2227460
                                                                                                                                            |                                                                                                                                            
cAGCGTTACTGCTAAGAAGTGGCATTGACCCCCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAt                                                                                                                                              <  2:734709/141‑1 (MQ=255)
cAGCGTTACTGCTAAGAAGTGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTGGCCGGTTGGTCTTTTTTTCCAACTACTCGAACAAAt                                                                                                                                              >  1:679816/1‑141 (MQ=255)
   cGTTACTGCTAAGAAGTGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTTCAAATGAt                                                                                                                                           >  1:523634/1‑141 (MQ=255)
   cGTTACTGCTAAGAAGTGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGAt                                                                                                                                           >  1:199140/1‑141 (MQ=255)
                   tGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTTTAATTACTCTTACAAATGATAGCAAGAGGAAAAACa                                                                                                                           >  2:164195/1‑141 (MQ=255)
                   tGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATTATAGCAAGTAGAAAAACa                                                                                                                           <  2:230244/141‑1 (MQ=255)
                   tGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACa                                                                                                                           <  1:650559/141‑1 (MQ=255)
                   gggAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACa                                                                                                                           <  1:214046/140‑1 (MQ=255)
                    ggAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACGAATGATAGCGAGTAgccaaataa                                                                                                                          >  1:568511/1‑133 (MQ=255)
                       aTTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTGGAAAAACAAGaa                                                                                                                       >  2:270892/1‑141 (MQ=255)
                       aTTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGAAAGCAAGTAGAAAAACAAGaa                                                                                                                       >  2:478120/1‑141 (MQ=255)
                         tGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAAc                                                                                                                     >  1:653526/1‑141 (MQ=255)
                           accaccTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTGGCCAGTTGGTATTTTTTACAAATGACGCTTAGAACTGATACGAAGTACAACAACAAAAAACGc                                                                                                                   >  1:694399/1‑141 (MQ=255)
                             ccccTTTTTGCCAAGCCATGTTACCTTCAATGATTCCACTCACGAAATCAAAAATCCATTCAACGATATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGACAGCAAGTAGAAAAACAAGAAACGCCg                                                                                                                 <  1:266147/139‑1 (MQ=255)
                              accTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGt                                                                                                                >  1:100886/1‑141 (MQ=255)
                              accTGTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCATACAAATGATAGCAAGTAGAAGAACAAGAAACGCCGt                                                                                                                >  1:555524/1‑141 (MQ=255)
                               ccTTTTTTCCACGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCGAATTACGCGTAGATTTGTTAGCGAGTGGACAAACatgtgagggcgta                                                                                                               >  1:294241/1‑129 (MQ=255)
                               ccTTTTTTCCAAGCCATGTTACCTTCGATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTa                                                                                                               <  2:40130/141‑1 (MQ=255)
                                  tttttCCAAGGTATTTTAGCTACAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGGTGGTCCTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACa                                                                                                            <  2:316726/141‑1 (MQ=255)
                                  tttttCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACa                                                                                                            <  1:67485/141‑1 (MQ=255)
                                  ttgttCCAAGCCATGTTACCTGCCATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTATTCAAATTCCGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACa                                                                                                            <  1:306486/138‑1 (MQ=255)
                                    tttCCAAGCCATGGTAGCTCCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCGAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAg                                                                                                          <  2:502581/141‑1 (MQ=255)
                                     ttCCAAGCCATGTTACCTTCAATGATCCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGt                                                                                                         <  2:598099/141‑1 (MQ=255)
                                      tCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAGAAACAAGAAACGCCGTAACAAGTa                                                                                                        >  2:265943/1‑141 (MQ=255)
                                        cAAGCCATGTTACCTTCAATGATTCCCCTCTCGAAATCAAAAATCCATTCAACGGAATTTTGTTTGCCAGTTGGTCTTTTTTTCAATTTTCCCGTACAAAGGATAGCAAGTAGAAAAACAAGAAGCGCCGTAACAAGTatc                                                                                                      >  1:21863/1‑141 (MQ=255)
                                         aaGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGGTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTatca                                                                                                     <  2:164755/141‑1 (MQ=255)
                                          aGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATACATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTatcat                                                                                                    <  1:195154/141‑1 (MQ=255)
                                           gCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTatcatc                                                                                                   <  1:701106/141‑1 (MQ=255)
                                                     cTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAGGAAGAAAATCAAGAAACGCCGTAACAAGTATCATCAATATACttt                                                                                         >  1:5940/1‑140 (MQ=255)
                                                        cAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATa                                                                                      <  2:776346/141‑1 (MQ=255)
                                                               tCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGaa                                                                               >  1:288326/1‑141 (MQ=255)
                                                               tCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGaa                                                                               >  2:375296/1‑141 (MQ=255)
                                                                ccccTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCCATATACTTGATAAATTGaaa                                                                              >  1:238017/1‑141 (MQ=255)
                                                                ccccTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGaaa                                                                              >  1:600852/1‑141 (MQ=255)
                                                                    tCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTATGCGTACAAATGATAGCAAGTAGCACAACAAGAAACGCCTTAACAAGTATCATCAATATACTTGGTAAATTGAAAACgc                                                                          >  2:2054/1‑140 (MQ=255)
                                                                      aCGAAATCAAAAATCCATTCAACTAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGata                                                                        <  2:535420/141‑1 (MQ=255)
                                                                      aCGAAATCAAAAATCCATCCAACGAAATTTTATTTGCCAGTTGGTCGTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGata                                                                        <  2:535527/141‑1 (MQ=255)
                                                                             cAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGAATCATCAATATACTTGATAAATTGAAAACGATATCAAAAc                                                                 >  1:553511/1‑141 (MQ=255)
                                                                               aaaaTCCATTCCACTACATTGTGTTAGCCCGTTGTCCTTTTTTTCAAATTACCCGTACAAATGATAGCAAATAGAAAAACAAGAAAAGCAGTAACAAGTATCATCATTATACTTGATAAATTGAAAACGATATCACAACCg                                                               <  2:633418/141‑1 (MQ=255)
                                                                                 aaTCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGGTAAATTGAAAACGATATAAAAACCGaa                                                             >  2:449000/1‑141 (MQ=255)
                                                                                 aaTCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGaa                                                             >  1:527710/1‑141 (MQ=255)
                                                                                  aTCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAAt                                                            <  2:160220/141‑1 (MQ=255)
                                                                                    ccATTCAACGAAAGTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATaa                                                          <  2:102835/141‑1 (MQ=255)
                                                                                       ttCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACTCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAAtt                                                       >  1:744203/1‑141 (MQ=255)
                                                                                                  tttgtttgCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATAAACTTGATAAATTGAAAAGGATATAAAAACCGCAAAAATTCAAACTCACGa                                            >  2:376271/1‑141 (MQ=255)
                                                                                                   ttgtttgCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAg                                           >  1:18138/1‑141 (MQ=255)
                                                                                                    tgtttgCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGc                                          <  1:657884/141‑1 (MQ=255)
                                                                                                    tgtttgCCAGTTGGTCTTTTTTTCAAATTACGCGTAAAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGc                                          <  1:588879/141‑1 (MQ=255)
                                                                                                     tttggCCAGTTGGTGTTTTTTTTAAATTACTCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCg                                         <  2:639523/137‑1 (MQ=255)
                                                                                                     gtttgCCAGTTAGTCGTGTTTTCAAATGACTCGTACAAACGATAGCAAGTAGAAAAACAAGAAACGTCGTAACAATTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCg                                         <  2:59652/141‑1 (MQ=255)
                                                                                                          ccAGTCGATCTCTTTTTCAAATAACGCGTACAAATGAAAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGAt                                    <  2:673625/141‑1 (MQ=255)
                                                                                                          ccAGATTGTCTTTTTTTCAAATTCCGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAAATCCAACTCACGAGCGGGGAt                                    <  1:477164/141‑1 (MQ=255)
                                                                                                           cAGTTGGTCTTTTTTTCAAATTACGCGTAAAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGAtt                                   <  2:725897/141‑1 (MQ=255)
                                                                                                                    tttttttCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATACATTGACTGCAATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGGTTTGTGATCCa                          >  1:739393/1‑141 (MQ=255)
                                                                                                                    tttttttCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCa                          >  1:34138/1‑141 (MQ=255)
                                                                                                                     ttttttCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATCTCAAAACCGAATAAATTCCAACTCACGAACGGGGATTTGTGATCCAt                         >  1:593674/1‑141 (MQ=255)
                                                                                                                        tttCAAATTACGGGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAAt                      <  1:74319/141‑1 (MQ=255)
                                                                                                                            acaTTACGCGTACAATGGATAGCAAGAAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAATGATATCAAAACCGTATAAATTCCAACTCACGAGCGGGAATTTGTGATCCATAATCTCa                  <  1:478120/139‑1 (MQ=255)
                                                                                                                            aaaTTACGCGTACAAATGCTAGCAAGTACAAAAAAAAGAAACGCCCTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCa                  <  1:19313/141‑1 (MQ=255)
                                                                                                                            aaaTTACGCGTACAAATGATAGCAAGCAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGTATTTGTGATCCATAATCTCa                  <  1:478149/141‑1 (MQ=255)
                                                                                                                            aaaTTACGCGTACAAAGGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCa                  <  2:653526/141‑1 (MQ=255)
                                                                                                                             aaTTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTAAc                 >  1:488643/1‑141 (MQ=255)
                                                                                                                             aaTTACGCGTACAAAAGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGGATCATCAATATACTTGATAATTTGAACTCGATATCTAAACCGAATAAATTCCAACTCACGAGCGTGGATTTGTGATCCATAAtgcgac                 >  2:102348/1‑136 (MQ=255)
                                                                                                                                 aCGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACctct             <  1:650971/141‑1 (MQ=255)
                                                                                                                                   gcgTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCtcttt           >  1:736835/1‑141 (MQ=255)
                                                                                                                                    cgTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCtctttc          <  1:120383/141‑1 (MQ=255)
                                                                                                                                        cAAAGGATAGCAAGTCGAACAAAAAGTAAAGCCGTAACAATTAGCAGCAAAATACTTGAGAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTaa      >  2:746792/1‑141 (MQ=255)
                                                                                                                                           aTGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTAattt   >  1:403700/1‑141 (MQ=255)
                                                                                                                                            tGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTAatttt  >  1:680903/1‑140 (MQ=255)
                                                                                                                                            tGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTAatttt  >  1:80851/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CAGCGTTACTGCTAAGAAGTGGAATTGACCACCTTTTTTCCAAGCCATGTTACCTTCAATGATTCCCCTCACGAAATCAAAAATCCATTCAACGAAATTTTGTTTGCCAGTTGGTCTTTTTTTCAAATTACGCGTACAAATGATAGCAAGTAGAAAAACAAGAAACGCCGTAACAAGTATCATCAATATACTTGATAAATTGAAAACGATATCAAAACCGAATAAATTCCAACTCACGAGCGGGGATTTGTGATCCATAATCTCACCTCTTTCTTAATTTA  >  CP000730/2227180‑2227460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: