Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 54.2% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT54.2% 48.6 / 192.5 157intergenic (+107/+1)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (39/33);  new base T (31/53);  total (71/86)
Fisher's exact test for biased strand distribution p-value = 3.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-02

Only 100 of 172 total aligned reads displayed.

AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016
                                                                                                                                            |                                                                                                                                            
atcTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              <  2:410365/139‑1 (MQ=255)
aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAACATAGAAGGTTCTGAAAAAAAAAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              <  2:448134/141‑1 (MQ=38)
aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACCTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAACACAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              <  2:55759/141‑1 (MQ=255)
  cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                                                            >  2:417709/1‑141 (MQ=255)
       taGTATAAAGTTTTTGGCGAAAAATGATTACTTGAATAAGATTTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                                                       <  2:80172/140‑1 (MQ=255)
       cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                                                       >  2:718520/1‑141 (MQ=255)
         gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                                                     >  1:546589/1‑141 (MQ=255)
            taAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTCACTTGCTAg                                                                                                                                  >  1:772572/1‑141 (MQ=255)
                   tttGGCGAAAAATGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                                           <  2:281577/141‑1 (MQ=255)
                   tgtGGCGAAAAATAAATACTTGAAAAAGAGCTAAAATACAATTTTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGGAAAAAAAGATAGCTACCCACTATTTTACCATGACATTGTTCGCTTAACTTGCTAGCatgatg                                                                                                                           <  1:188553/139‑1 (MQ=21)
                      ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                                                        >  2:687987/1‑141 (MQ=255)
                       ttttttttttaTTACTTGAACAAGAGCTAAAATACAATTGGGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATATCTAACCACTAATTTATCATCTCAGTGTTCACTTAACTTGCTACCATGATGCTaa                                                                                                                       >  2:518972/11‑141 (MQ=18)
                              aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                                                                <  1:543815/141‑1 (MQ=255)
                                         aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                                                                                     >  2:164649/1‑141 (MQ=255)
                                                      acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTCATTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                      >  1:570813/15‑141 (MQ=11)
                                                      acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                      >  1:67117/15‑141 (MQ=11)
                                                       caaaagagtatcttaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     <  1:690303/128‑1 (MQ=11)
                                                       aaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGGTAGTCACTAATATATCCTGTCAGTGTGCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAag                                                                                     <  2:398604/128‑1 (MQ=11)
                                                         aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                   >  1:473655/12‑141 (MQ=11)
                                                          aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAgaga                                                                                  >  1:477332/11‑141 (MQ=11)
                                                           agagtattttaataaAAATTGTGGTTCTGTTGCCAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:10018/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:419643/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:150579/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:11685/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:780424/132‑1 (MQ=11)
                                                            gattattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  1:251949/133‑1 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  1:365719/133‑1 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:93793/133‑1 (MQ=11)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:122288/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 >  1:398352/1‑141 (MQ=255)
                                                             tgtgAATTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:712039/141‑1 (MQ=255)
                                                             ggtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGCAAAAACATATAGCTAACCACTAATTTATCATGTCAGTCTTCGCTTAACTTGCTAGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTGAATCTGCAGCGAc                                                                                 >  2:588483/2‑141 (MQ=14)
                                                             agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTTAGGAGACCt                                                                               >  2:509622/8‑141 (MQ=11)
                                                             agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:572279/134‑1 (MQ=11)
                                                             agtattttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:143336/134‑1 (MQ=11)
                                                             agtattttaataaAAATGATAGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               >  1:476811/8‑141 (MQ=11)
                                                              gtattttgataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  2:751393/133‑1 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCAGTTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTg                                                                              >  1:380189/7‑141 (MQ=11)
                                                               tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:244772/141‑1 (MQ=255)
                                                               tattttaagaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:224420/136‑1 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTGACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAtgaggactgagg                                                                           >  1:58427/4‑133 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  1:687879/138‑1 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTCCTAGCATGATGCTAATTTCGTGGCATGGGGAAAACCCGTACATCTGAAGAGAACTGAgg                                                                           >  2:195721/4‑141 (MQ=11)
                                                                  tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  1:85120/139‑1 (MQ=11)
                                                                  tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:770750/139‑1 (MQ=11)
                                                                  tttaataaAAATGATGGTGCTGTTGCAAAGTAAAAAATATAGCTCATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:687952/139‑1 (MQ=11)
                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                         <  2:627242/140‑1 (MQ=11)
                                                                   cTAATAAAAATAGAAGGTTCTGTAACAAAAAAAAAAAATATAGCTAACCACTATTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  2:435088/141‑1 (MQ=14)
                                                                      ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  1:322063/1‑141 (MQ=11)
                                                                         aaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  2:307313/141‑1 (MQ=35)
                                                                               gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTCTTTTtatat                                                               >  1:329383/1‑141 (MQ=14)
                                                                                                                aatcACTAATATATCATGTCCGTATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTCGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATAGATTCAATACCTTCTAAAGTa                              <  1:497147/138‑1 (MQ=1)
                                                                                                                            tacacgTCAGTGCTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTa                  <  1:291518/136‑1 (MQ=0)
                                                                                                                                   ccgtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCCTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           <  2:31408/139‑1 (MQ=11)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           >  2:735552/1‑141 (MQ=2)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAACTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           >  1:215644/1‑141 (MQ=2)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:424798/141‑1 (MQ=11)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           >  1:605658/1‑141 (MQ=11)
                                                                                                                                   cAGTGCTGGTTTAACTTTCTAGGCTGATGCTAATTTCGTGGCATGGTGAAAATTCGTAGAGCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  2:598215/141‑1 (MQ=11)
                                                                                                                                    agcgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  1:218284/138‑1 (MQ=9)
                                                                                                                                    aGTGTTCGTTTATCTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  2:182266/141‑1 (MQ=9)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCCATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  2:377108/1‑141 (MQ=255)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:635328/1‑141 (MQ=9)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  1:251425/141‑1 (MQ=9)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGGGTAAACACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         >  2:357935/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         <  1:172779/141‑1 (MQ=255)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         <  2:467067/141‑1 (MQ=17)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATAAGTAGATTTGAAGAAACCTGCTGTTCTTTTTATATAGAGCGTAAAGACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTTATACtt         >  2:716532/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:324663/1‑141 (MQ=17)
                                                                                                                                     gtggTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  2:401053/1‑141 (MQ=12)
                                                                                                                                     gtgaTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATCCCTTTTCACGTATTCTTTGCTGTATTGATACtt         >  2:53368/1‑141 (MQ=11)
                                                                                                                                     ggtttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         <  1:126548/138‑1 (MQ=12)
                                                                                                                                     acgagcacTTAACTTGCTAGCATGATGCTAATCCCGAGGCATGCCGAAAATCCGTAGATCTGAAGAGACCAGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTAAGCTGAATTGATACtt         >  2:683354/8‑141 (MQ=11)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGGTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  2:214935/1‑141 (MQ=11)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCGGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTGTTCTTTTCTATTTTGATACttt        >  2:317199/1‑141 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTCATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATACAGCGTACATACATTACATACCTTTTACAGTATTATTTGCCGCATTGATACTTTg       >  1:161214/1‑141 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       >  2:729820/1‑141 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  1:169088/141‑1 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACTTTg       <  1:575974/141‑1 (MQ=255)
                                                                                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      <  2:618002/141‑1 (MQ=255)
                                                                                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      >  2:393151/1‑141 (MQ=255)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:265078/141‑1 (MQ=32)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      >  2:57287/1‑141 (MQ=17)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:565382/141‑1 (MQ=17)
                                                                                                                                         tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCCGCGGTGCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  2:475428/141‑1 (MQ=11)
                                                                                                                                         tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  1:344342/141‑1 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAACCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATATAGCGTAAATACATTTAATACCTTTTAAAGTAGGCTTTGCTGTATTGATACTTTGATa    <  2:3718/141‑1 (MQ=11)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:635540/1‑141 (MQ=32)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:27881/1‑141 (MQ=32)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGTTa    >  2:504920/1‑141 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:50485/1‑141 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTATTTTTATATAGAGCGTAAATACCTTCAATACCTTTTAAAGTATTATTTGCTGTATTGAAACTTTGATa    >  2:667402/1‑141 (MQ=255)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  2:544905/1‑141 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:463792/141‑1 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   >  2:484936/1‑141 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:463598/141‑1 (MQ=17)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  2:286607/141‑1 (MQ=32)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:424160/141‑1 (MQ=17)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  >  1:434180/1‑141 (MQ=17)
                                                                                                                                            cTTAACTCGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTTGATAcc  <  1:790835/141‑1 (MQ=12)
                                                                                                                                            |                                                                                                                                            
AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: