Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 55.4% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA55.4% 132.4 / inf 657intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (187/105);  new base A (181/183);  total (368/289)
Fisher's exact test for biased strand distribution p-value = 2.69e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-02

Only 100 of 661 total aligned reads displayed.

ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAA  >  CP000730/76182‑76462
                                                                                                                                            |                                                                                                                                             
   gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGaa                                                                                                                                            >  1:111865/1‑141 (MQ=32)
     ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                                                          >  2:198885/1‑141 (MQ=17)
      taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGACATATTAAGAGAACgc                                                                                                                                         >  2:719881/1‑140 (MQ=12)
      taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                                                         <  1:549999/141‑1 (MQ=17)
      taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgg                                                                                                                                         >  2:240233/1‑140 (MQ=12)
       aactttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                                                        <  1:318366/138‑1 (MQ=255)
        aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                                                       >  2:658656/1‑141 (MQ=17)
        aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGTTATGCATTGAGCTATCGTGAGATATCTGAAATATTAATAGAACgtgg                                                                                                                                       <  2:507532/141‑1 (MQ=11)
          tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                                                                                     <  1:210329/141‑1 (MQ=17)
           ttAACAAAAAGGAGTCTTCTGCATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATCTGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                                                                                    >  2:542335/1‑141 (MQ=12)
            tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                                                                                   >  2:48108/1‑141 (MQ=17)
             aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATCTAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGTTATGCATTGAGTTATCGTGATATAGATGACATATTAAGGGCACGTGgtgtga                                                                                                                                  >  1:23895/1‑139 (MQ=11)
             aaCAAAAAGGAGTCTTCTGTATGAACGATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                                                  >  2:491032/1‑141 (MQ=12)
              aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAATATTAAGGGAACGTGGTGTaaa                                                                                                                                 >  2:699537/1‑141 (MQ=17)
               cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaag                                                                                                                                >  2:307445/1‑140 (MQ=17)
                aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                                                               >  2:459713/1‑141 (MQ=17)
                 aaaaGGCGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                                              <  1:623809/141‑1 (MQ=12)
                 aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                                              >  1:283332/1‑141 (MQ=17)
                  aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaaaggat                                                                                                                             >  2:372503/1‑137 (MQ=17)
                   aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtc                                                                                                                            <  1:738905/141‑1 (MQ=17)
                     ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcat                                                                                                                          >  1:384319/1‑141 (MQ=17)
                     ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTGATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                                                          <  1:327640/141‑1 (MQ=17)
                      gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAATGATGTTATCACTGTATCCGTTGGCTACTATCTAAGATATGCATTGGGCTATCGTGATCTATCTGAAATATTAAGAGAACGTGGTGGAAACGTtcatc                                                                                                                         >  1:147530/1‑141 (MQ=11)
                      gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                                                         >  1:155218/1‑141 (MQ=17)
                      gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                                                         >  1:758431/1‑141 (MQ=17)
                       aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                                                        >  1:394759/1‑141 (MQ=17)
                        gTCTTCTGTAGGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                                                                                       >  1:571024/1‑141 (MQ=17)
                         tcttctGTATGAACTATTTCAGATATAAACAATTTAAGGAGGATTTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGAGGTGTAAACGTtcatcaat                                                                                                                      >  1:604800/1‑139 (MQ=11)
                         tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTCTCAATGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTTAAATATTAATATAACGTGGTGTAAACGTTCATCAtt                                                                                                                      >  2:68947/1‑141 (MQ=11)
                           ttctGTACGAACCATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCa                                                                                                                    <  2:416256/141‑1 (MQ=255)
                              tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTGAGAGAACGTGGTGTAAACGTTCATCATTCAGCg                                                                                                                 >  1:348949/1‑141 (MQ=255)
                               gTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                                                <  1:160613/141‑2 (MQ=255)
                               gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATCTATCTGAAATATTAAGAGAACGTCGTGTAAACGTTCATCATTCAACga                                                                                                                <  2:268303/141‑2 (MQ=255)
                                 aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                              <  2:726277/141‑1 (MQ=17)
                                  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                             <  1:531910/141‑1 (MQ=255)
                                    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                           >  1:443583/1‑141 (MQ=12)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                          >  1:714303/1‑141 (MQ=17)
                                     aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAACGAGAACAAGCATAAAAAAGAAGGCAACGTGGTGTAAACGTTCATCATTCAACGGTCTAcc                                                                                                          >  2:359597/1‑141 (MQ=11)
                                       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                        <  1:785447/141‑1 (MQ=17)
                                       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGGAACCGTTCATCCTTCAACGATcacacgt                                                                                                        >  1:252617/1‑135 (MQ=11)
                                         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                      <  1:674357/141‑1 (MQ=12)
                                          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGAAGTTGGCTAATATCTAAGATATGAATAGAAAACACGTGATATATATGAAATATTAAGAGAAAGCGGTGTAAACGTTCATAATTCAACGATCTACTGtgag                                                                                                     >  2:219575/1‑138 (MQ=2)
                                           tCAGATATAAACAGTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                    <  1:643931/141‑1 (MQ=255)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCATCATTCAACGGTCTACCGTTggg                                                                                                    >  1:101615/1‑141 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCATTggg                                                                                                    >  2:128963/1‑141 (MQ=17)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                    <  1:225327/141‑1 (MQ=32)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTgggg                                                                                                    >  2:207964/1‑141 (MQ=255)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                    >  1:59428/1‑141 (MQ=12)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                    >  1:304812/1‑141 (MQ=12)
                                           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                    >  2:400429/1‑141 (MQ=12)
                                           tCAGATATAAACAAGTTAACAATGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                    <  1:191528/141‑1 (MQ=11)
                                            cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                                                                   >  2:557578/1‑141 (MQ=17)
                                                 ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaaa                                                                                              >  2:275954/1‑140 (MQ=17)
                                                  taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                             >  1:72304/1‑141 (MQ=17)
                                                  taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                             <  2:566722/141‑1 (MQ=32)
                                                   aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                                                            <  1:767083/141‑1 (MQ=255)
                                                   aaaCAATATAACACGGACTTTATCACTGTAGCCGTTCGCTACTAACTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGATAACGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGTTCAAGaa                                                                                            <  2:613061/141‑1 (MQ=11)
                                                   aaaCAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                                                            <  2:387153/141‑1 (MQ=255)
                                                    aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                                                           >  1:177122/1‑141 (MQ=17)
                                                     aCAATTTAACAAGGATGTTATTACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                                                          <  2:336477/141‑1 (MQ=255)
                                                      cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTACCGTTAGGTGCAAGAatat                                                                                         >  2:432003/1‑141 (MQ=12)
                                                       aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                        <  1:692118/141‑1 (MQ=12)
                                                         tttAACAAGGATGTTATCACTGTAGGCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                      <  2:178595/141‑1 (MQ=11)
                                                          ttAACCAGGATGTTATCACTGGAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                     <  2:639006/141‑1 (MQ=11)
                                                          ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                     >  1:264713/1‑141 (MQ=17)
                                                          ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAACGTTGGGTTCACGAATATGccc                                                                                     >  2:34931/1‑141 (MQ=12)
                                                            aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                   >  2:58301/1‑141 (MQ=17)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTGGGTTCTAGAATATGCCCCaa                                                                                  >  1:422348/1‑141 (MQ=12)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATACCCCaa                                                                                  >  2:147328/1‑141 (MQ=12)
                                                             aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAATGAGTTATCGTGATATATCTGAAATATTCAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                  >  1:366096/1‑141 (MQ=12)
                                                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                  >  1:788972/1‑141 (MQ=255)
                                                              cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATACCTGAAATCTTAAGGGAACGTGGTGGAAACGCTCATCATTCAACGGTCTACCGTTGGGTTCCAGAATATGCCCCAAt                                                                                 >  2:296728/1‑141 (MQ=11)
                                                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                >  2:537074/1‑141 (MQ=12)
                                                                   aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                            >  1:748408/1‑141 (MQ=255)
                                                                   aTGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACAGTAAATCCCAAGTATATGCCCCAATTTtat                                                                            <  2:760928/141‑1 (MQ=11)
                                                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                           >  1:498063/1‑141 (MQ=255)
                                                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGAGCTGCCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                          >  1:50535/1‑141 (MQ=11)
                                                                             tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttgg                                                                  >  2:519718/1‑139 (MQ=25)
                                                                              gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                 <  2:110951/141‑1 (MQ=25)
                                                                              gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                 <  2:454350/141‑1 (MQ=255)
                                                                                tgCCGTTGGCTACTATCTAAGATATGCATTGATCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                               <  2:595024/140‑1 (MQ=255)
                                                                                  ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                             >  2:442065/3‑141 (MQ=25)
                                                                                  ccGTTGGCTACTATCTAAGATATGCATTGGGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATAAGAACCAAGTTTATATCAAATTTGgaaga                                                             <  2:169276/141‑1 (MQ=11)
                                                                                   tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                            >  2:741460/2‑141 (MQ=25)
                                                                                   cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgacgaa                                                            >  1:51414/1‑141 (MQ=255)
                                                                                        gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGCGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAGTTTATATCAAATTTGGAAGAAAAAGc                                                       >  1:481942/1‑141 (MQ=25)
                                                                                        gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                       <  1:341602/141‑1 (MQ=255)
                                                                                         ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                      <  2:90328/141‑1 (MQ=35)
                                                                                          tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                     <  1:556211/141‑1 (MQ=35)
                                                                                          tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                     <  2:162432/141‑1 (MQ=34)
                                                                                           actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                    <  1:89635/141‑1 (MQ=35)
                                                                                              aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAGATTTGGAAGAAAAAGCATaaaa                                                >  1:377685/1‑141 (MQ=25)
                                                                                                  aaGATATGCATTGAGTCATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCt                                            <  1:656268/141‑1 (MQ=38)
                                                                                                   aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                           >  2:315750/1‑137 (MQ=17)
                                                                                                   aGATATGCATTAAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                           >  2:781126/1‑137 (MQ=14)
                                                                                                    gATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcta                                          >  1:460218/1‑136 (MQ=11)
                                                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                        >  1:125058/1‑134 (MQ=11)
                                                                                                                          tatatCTGAGATATTAAGGGAACGTGGTGTAACCGATCATCATTCCACGGTCTACCGGTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATgag                    >  1:577824/1‑141 (MQ=14)
                                                                                                                                       ttAAGGAAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATAACAAAGCTAATAACAAATGGCGTATTGATGATACATACATCaaa        >  2:653886/1‑141 (MQ=11)
                                                                                                                                          aGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATaa    >  1:634021/1‑141 (MQ=39)
                                                                                                                                            |                                                                                                                                             
ATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAA  >  CP000730/76182‑76462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: