Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 56.6% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC56.6% 222.3 / inf 706intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (191/115);  new base C (186/210);  total (380/326)
Fisher's exact test for biased strand distribution p-value = 5.15e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 708 total aligned reads displayed.

AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATG  >  CP000730/76158‑76431
                                                                                                                                         |                                                                                                                                        
aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                                                                                                                                       >  2:785234/1‑141 (MQ=0)
 ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                                                                                                                                      >  1:335508/1‑141 (MQ=35)
   ttCTGTTGGAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                                                                                                                                    <  2:336042/141‑1 (MQ=25)
                     tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                                                  >  1:282971/3‑141 (MQ=14)
                          aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                                             <  2:425/139‑4 (MQ=255)
                          aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                             <  1:249946/141‑1 (MQ=17)
                               aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                        <  1:300977/141‑1 (MQ=12)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                       >  2:658656/1‑141 (MQ=17)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                       <  1:714934/141‑1 (MQ=12)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                       <  2:736066/141‑1 (MQ=12)
                                 ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggg                                                                                                      >  2:587760/1‑140 (MQ=17)
                                    tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                   >  1:113676/1‑141 (MQ=17)
                                     aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                  <  1:752690/141‑1 (MQ=17)
                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaag                                                                                                >  2:307445/1‑140 (MQ=17)
                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACCTGGTGTAAAc                                                                                                <  1:570979/141‑1 (MQ=255)
                                        aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTGAGAGAACGTGGTGTAAGCg                                                                                               >  1:503901/1‑141 (MQ=11)
                                          aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGtt                                                                                             >  2:176050/1‑141 (MQ=12)
                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                            >  1:160180/1‑141 (MQ=17)
                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                            >  1:723536/1‑141 (MQ=17)
                                              gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAATGATGTTATCACTGTATCCGTTGGCTACTATCTAAGATATGCATTGGGCTATCGTGATCTATCTGAAATATTAAGAGAACGTGGTGGAAACGTtcatc                                                                                         >  1:147530/1‑141 (MQ=11)
                                              gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                         >  1:155218/1‑141 (MQ=17)
                                                gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGAGATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                                                       <  1:740177/141‑1 (MQ=17)
                                                 tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTAAGAGAACGGGGGGTAAAGATACATCAtt                                                                                      >  2:346494/1‑141 (MQ=11)
                                                  cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                                                     >  2:684038/1‑141 (MQ=17)
                                                  cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                                                     >  1:65325/1‑141 (MQ=17)
                                                    tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa                                                                                   >  2:630153/1‑141 (MQ=17)
                                                    tatGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTCGTCCTGTGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                                                   <  1:375075/139‑1 (MQ=11)
                                                       gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATCTATCTGAAATATTAAGAGAACGTCGTGTAAACGTTCATCATTCAACga                                                                                <  2:268303/141‑2 (MQ=255)
                                                          tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                             <  1:531910/141‑1 (MQ=255)
                                                            aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCTACGGTCTAc                                                                           >  2:15816/1‑141 (MQ=17)
                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGATATCACTGTAGCTAGTGGCTACTATATAAGATATGAATTGAGTTATCGTGAGATATATGAAAGATTAAGGGAACGTGGTGTAAAAGTTACTCATTCAACGGTCTAcc                                                                          >  2:384178/1‑141 (MQ=11)
                                                             aCTATTTCAGATAGAAACAATTTAACAAGGATGTTATCACGGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATGCAACGGTCTAcc                                                                          <  1:557607/141‑1 (MQ=255)
                                                                ttttCAGATATAAACAATTTAACAAGGACGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                       <  2:516176/140‑1 (MQ=255)
                                                                  ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGAAGTTGGCTAATATCTAAGATATGAATAGAAAACACGTGATATATATGAAATATTAAGAGAAAGCGGTGTAAACGTTCATAATTCAACGATCTACTGtgag                                                                     >  2:219575/1‑138 (MQ=2)
                                                                   tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCATCATTCAACGGTCTACCGTTggg                                                                    >  1:101615/1‑141 (MQ=17)
                                                                   tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTgggg                                                                    >  2:207964/1‑141 (MQ=255)
                                                                   tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                    >  1:237906/1‑141 (MQ=12)
                                                                   tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                    >  1:380551/1‑141 (MQ=12)
                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                  >  1:758979/1‑141 (MQ=17)
                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                  >  1:358211/1‑141 (MQ=17)
                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                  >  2:143000/1‑141 (MQ=255)
                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                  <  1:681254/141‑1 (MQ=255)
                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGAAATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTAATCATTCAACGATCTACCGTTGGGtt                                                                  >  2:196388/1‑141 (MQ=11)
                                                                     aGAAATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTCGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                  <  1:324949/141‑1 (MQ=11)
                                                                        tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCaa                                                               >  2:127456/1‑141 (MQ=12)
                                                                          taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                             <  1:206836/141‑1 (MQ=255)
                                                                          taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                             >  1:459970/1‑141 (MQ=255)
                                                                            aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                           >  1:187013/1‑141 (MQ=12)
                                                                            aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                           <  2:752708/141‑1 (MQ=12)
                                                                             aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                          <  1:785745/141‑1 (MQ=255)
                                                                               aaTTTAACATGGATTTTATCACTGTCGCCGTTGGCTACTATCTAAGATAGGCATTGAGCTAGCGTGATATATCTGAAATATTAAGAGAACGCGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                        <  1:26298/141‑1 (MQ=11)
                                                                                   tAACAAGGATGTTAACACTGTAGCTGTTGGCTACTATCTAAGATGTGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                    >  1:433820/1‑141 (MQ=12)
                                                                                     aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                  >  1:610400/1‑141 (MQ=17)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                  >  2:293595/1‑141 (MQ=255)
                                                                                       aaGGATGTTATCACTGTAGCTGTTGGCTACTCTCTAAGATATGAATTGAGTTATCGTGATATATCGGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGCTTGGATCAAGAATATGCCCCAAtt                                                >  2:6016/1‑141 (MQ=11)
                                                                                        aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                               <  2:562507/141‑1 (MQ=12)
                                                                                         ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCAtttt                                              >  1:342/1‑141 (MQ=17)
                                                                                          gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCGCCAATTTta                                             <  1:579097/141‑1 (MQ=255)
                                                                                          gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                             <  1:635766/141‑1 (MQ=255)
                                                                                          gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                             <  2:722119/141‑1 (MQ=12)
                                                                                           aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                            >  2:240445/1‑141 (MQ=255)
                                                                                           aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                            <  2:411384/141‑1 (MQ=16)
                                                                                            tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                           >  1:498063/1‑141 (MQ=255)
                                                                                            tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                           <  1:407825/141‑1 (MQ=255)
                                                                                               tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                        >  2:577968/1‑141 (MQ=25)
                                                                                               tATCACTGTAGCCTTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                        <  1:390422/141‑1 (MQ=11)
                                                                                               tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                        <  1:310558/141‑1 (MQ=255)
                                                                                                aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTATACCGTTGGGTTCAAGAATATGCACCCATTTTATATCaa                                       >  2:763559/1‑141 (MQ=255)
                                                                                                aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                       >  1:598751/1‑141 (MQ=35)
                                                                                                      ttAGCCGTTGGCTAATATATAAGAAATGCATTGAGCTATCTTGATATATCTTAAATATTAAGAGATCGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                 <  2:505126/140‑1 (MQ=11)
                                                                                                       tAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                <  2:551670/141‑1 (MQ=25)
                                                                                                        aGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                               <  2:372100/141‑1 (MQ=25)
                                                                                                         gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                              <  2:539637/138‑1 (MQ=25)
                                                                                                         gCCGTTGGCTACTAATGAAGATATGCATTTAGCTATCGTGATATACCTGAAATATTAAGAGAACGTGTTGTAAACGTTCATCATTCACCGATTTACCGTTGGGTTCAATAATATGCCCCAAGTTTATATCAAATTTGgaag                              <  2:275767/141‑1 (MQ=11)
                                                                                                          ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATAATTCACCGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTCTATATCAAATTTGgaaga                             <  2:71066/139‑1 (MQ=255)
                                                                                                          ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                             >  2:45866/1‑141 (MQ=255)
                                                                                                          ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                             >  2:43892/1‑141 (MQ=255)
                                                                                                           cGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                            >  2:100925/1‑141 (MQ=35)
                                                                                                           cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgacgaa                            >  1:51414/1‑141 (MQ=255)
                                                                                                           cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaataa                            >  2:167615/1‑141 (MQ=255)
                                                                                                            gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGGTCAAGATTATGCCCCAATTTTATATCAAATTTGGAAGaaa                           >  2:450630/1‑141 (MQ=11)
                                                                                                                gctaacaTCTAAGATATGCACTGAGTTAACGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTTGAAGAAAAAGc                       <  2:399516/135‑1 (MQ=11)
                                                                                                                gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGCGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAGTTTATATCAAATTTGGAAGAAAAAGc                       >  1:481942/1‑141 (MQ=25)
                                                                                                                 ctagtaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                      <  2:528712/141‑1 (MQ=11)
                                                                                                                 ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                      <  2:90328/141‑1 (MQ=35)
                                                                                                                  tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATTTGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                     >  2:653841/1‑141 (MQ=25)
                                                                                                                  tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                     <  1:76849/141‑1 (MQ=255)
                                                                                                                  tactaTCTAAGATATGCATGTGGCTATCTTGATATACCTGAAATATAAAGAGAACGTCGTGCAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                     <  2:475520/141‑1 (MQ=11)
                                                                                                                   actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                    <  1:169813/141‑1 (MQ=255)
                                                                                                                    ctaTCTAAGATATGCGTTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                   <  1:658266/141‑1 (MQ=255)
                                                                                                                       tCTAAGATATGCATTGAGCTATCGTTATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                <  1:552501/141‑1 (MQ=255)
                                                                                                                        cTAAGATATGCATTGAGCTATCGTGAGATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa               >  2:488840/1‑140 (MQ=11)
                                                                                                                           aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct            >  2:315750/1‑137 (MQ=17)
                                                                                                                           aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATAAAATTTGTAAGAAAAACCATaaaaaagct            >  1:8760/1‑137 (MQ=255)
                                                                                                                           aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGAAACGTCGTTTAAACGTTCATCATTCAACGCACTGCCGTTGGGTTAAACGCGATAAACCGATTGTATAACAATTTTAAAACAAAAAGCATaaaaaaact            >  1:694637/1‑137 (MQ=2)
                                                                                                                            gATATGCATTGAGCTCTCGTGATATATCTGAAATATTAAGAGCACGTGGTGTAAACTTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctt           <  1:22029/141‑6 (MQ=11)
                                                                                                                             atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAGGAAAAAGCCTaaaacagctgt          >  1:624481/1‑134 (MQ=11)
                                                                                                                              tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat         >  1:125058/1‑134 (MQ=11)
                                                                                                                                tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTTTATATCCCATTTGGAAGAAAACGCATAAAAAGCTAATTAc       <  2:694516/141‑1 (MQ=255)
                                                                                                                                     tGAGTTATCGTGATGGATCTGAAATATTGAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTTTTTATTGAATGTGGTGGAAAAAGCATAAAAAGCTAATTACAAATg  >  2:357693/1‑141 (MQ=11)
                                                                                                                                         |                                                                                                                                        
AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATG  >  CP000730/76158‑76431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: