Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 32.0% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT32.0% 544.5 / inf 601intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (198/207);  new base T (128/63);  total (329/272)
Fisher's exact test for biased strand distribution p-value = 3.33e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 620 total aligned reads displayed.

AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405
                                                                                                                                            |                                                                                                                                            
  ggCAATTAATTTAATAATTATAGCAAAATATAGGTACTGTTGCAAAGTTGAATCTATAGTATAATATTAACAAAAAGGAGTCTGCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                                                                                                            >  2:228296/1‑141 (MQ=255)
                         cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTCTCACTGTAACTGTTGGCCACTATCTAAGATATGCAt                                                                                                                     >  1:530289/9‑141 (MQ=1)
                         cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                                                                                                     >  1:156821/9‑141 (MQ=11)
                             atatAGGTTCTGGTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                 <  1:9816/141‑1 (MQ=255)
                             aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                 <  1:17344/137‑1 (MQ=9)
                              aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                                                                                                                >  1:206619/4‑140 (MQ=2)
                               tgaGGTTCTGATGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                                                                                                               <  2:550254/139‑1 (MQ=2)
                                gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                                                                                                              >  2:267132/2‑141 (MQ=14)
                                  ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                                                                                                            <  2:76154/141‑1 (MQ=35)
                                    ttCTGTTGCAAAGTTGAATCTCTAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                                                                                                          <  1:253568/141‑1 (MQ=25)
                                           gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat                                                                                                   >  2:250451/1‑141 (MQ=34)
                                                      tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                        >  1:306534/3‑141 (MQ=11)
                                                      tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                        >  1:282971/3‑141 (MQ=14)
                                                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  1:70438/140‑1 (MQ=12)
                                                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  1:714771/140‑1 (MQ=12)
                                                       ttataatataaTTTTAACAAAAAGGAGGTTTCTGTATGAACTAGTTCGGATATAAACCATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  2:358064/140‑1 (MQ=11)
                                                       ttataatataaTTGTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  2:317191/140‑1 (MQ=12)
                                                        tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                      >  1:618780/1‑141 (MQ=17)
                                                         ttaatataATTTTAACAAAAAGGAGTCTTCTTTATGAACTATTTTAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                     <  2:512904/137‑2 (MQ=11)
                                                           aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                   <  1:558478/139‑4 (MQ=12)
                                                               taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                               <  1:549999/141‑1 (MQ=17)
                                                                aactttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                              <  1:318366/138‑1 (MQ=255)
                                                                  ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggg                                                                            >  2:587760/1‑140 (MQ=17)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                           <  1:727694/141‑1 (MQ=17)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                           <  1:645841/141‑1 (MQ=12)
                                                                    ttAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                          <  1:74340/141‑1 (MQ=17)
                                                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                         >  1:113676/1‑141 (MQ=17)
                                                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                         >  2:471990/1‑141 (MQ=17)
                                                                      ttcAAAAAGGCGTCTTCTTTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTTTCGTGATTTTTCTGATATATTAAGAGAACGTGGTGTaa                                                                        >  2:172876/3‑141 (MQ=11)
                                                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                        >  2:421750/1‑141 (MQ=17)
                                                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAc                                                                      <  1:459776/141‑1 (MQ=12)
                                                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTGAGAGAACGTGGTGTAAGCg                                                                     >  1:503901/1‑141 (MQ=11)
                                                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                     >  2:601043/1‑141 (MQ=17)
                                                                           aaaGGAGTCTTCTGTATGAACTATTTTAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTCTCGTGATATATCTGACATATTAAGGGAACGGGGTGTAAAAGtt                                                                   >  2:489928/1‑141 (MQ=11)
                                                                           aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGtt                                                                   >  2:92769/1‑141 (MQ=17)
                                                                           aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGtt                                                                   >  1:151735/1‑141 (MQ=17)
                                                                           aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                   >  1:230552/1‑141 (MQ=17)
                                                                             aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGATGTTATAACTGTACACGTTGGATCATACAAAACACATGCATTGAGGTATCGTGCTATATCTGAACTATGAAGAGAACGTGGTGTAAACGttaa                                                                 >  2:656987/1‑139 (MQ=11)
                                                                              ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                <  1:755926/141‑1 (MQ=17)
                                                                              ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcat                                                                >  1:384319/1‑141 (MQ=17)
                                                                               gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                               <  2:204502/141‑1 (MQ=17)
                                                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                              >  1:394759/1‑141 (MQ=17)
                                                                                aGTCCTCTGTACTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGCACGTGGTGTAAACGTtcatca                                                              <  2:538207/141‑1 (MQ=11)
                                                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTtaatctt                                                             >  1:298726/1‑139 (MQ=12)
                                                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTACGAAAAAGTGGTGTAAACGTTCAGCAtt                                                            >  2:752560/1‑141 (MQ=11)
                                                                                    gggtgttggAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTAATCATTCa                                                          >  2:398997/9‑141 (MQ=11)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCGTTCAACga                                                      >  2:721479/1‑140 (MQ=255)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                      <  2:62912/141‑2 (MQ=12)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                      <  2:578903/141‑2 (MQ=17)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATGTATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                 >  2:433524/1‑141 (MQ=255)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                 >  1:147466/1‑141 (MQ=12)
                                                                                              aCTATTTCAGATATAAACAATTTAACAAGGATGATATCACTGTAGCTAGTGGCTACTATATAAGATATGAATTGAGTTATCGTGAGATATATGAAAGATTAAGGGAACGTGGTGTAAAAGTTACTCATTCAACGGTCTAcc                                                >  2:384178/1‑141 (MQ=11)
                                                                                                tATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                              <  2:292759/141‑1 (MQ=255)
                                                                                                tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                              <  1:416145/141‑1 (MQ=255)
                                                                                                caTTTCAGATATAAACAATTTAACAAGGATGTTATCACCGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                              <  1:217382/140‑1 (MQ=11)
                                                                                                 aTTTCAGATATAAACAATATAACCAGGTTGTTATTACTGTAGCTGTTCGCTACAATGTGGAGGGTGCATGGTGTTAGCGGGATATGTCTTAATTAGTTACTTGACGTGGTGGAAACGTTCATCATTCAACGGTCTACCGtt                                             <  2:458365/141‑1 (MQ=2)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                           >  2:339524/1‑141 (MQ=17)
                                                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCATCATTCAACGGTCTACCGTTggg                                          >  1:101615/1‑141 (MQ=17)
                                                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                          <  1:761238/141‑1 (MQ=12)
                                                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                          >  1:241369/1‑141 (MQ=12)
                                                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGt                                         >  2:301284/1‑141 (MQ=255)
                                                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                        <  1:681254/141‑1 (MQ=255)
                                                                                                          ataAACCACTTAACAAGGATGTTATCACTGTATCAGTGTGGAACTATCGAAGATATGCATTGAGCTATCGTGATATATCCGAAACATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                    <  1:18819/141‑1 (MQ=11)
                                                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGCTGGGTTCAAg                                    <  1:571419/141‑1 (MQ=11)
                                                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGa                                   >  1:164243/1‑141 (MQ=17)
                                                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                   <  2:67332/141‑1 (MQ=12)
                                                                                                            aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                  >  2:777717/1‑141 (MQ=17)
                                                                                                            aaaCAATGTAACCAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                  <  2:194917/141‑1 (MQ=12)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTCAGCTACTATCTAAGATATGCCTTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGGAAACGCTCATGATTCAGGCATCTACCGTTggggacaaaaga                                 >  2:135804/1‑132 (MQ=11)
                                                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                >  2:408893/1‑141 (MQ=12)
                                                                                                                aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                              >  2:447522/1‑141 (MQ=255)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCACTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                            <  1:123422/141‑1 (MQ=255)
                                                                                                                   ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                           >  1:264713/1‑141 (MQ=17)
                                                                                                                   ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                           <  2:211573/141‑1 (MQ=32)
                                                                                                                    tAACAAGGATGTTAACACTGTAGCTGTTGGCTACTATCTAAGATGTGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                          >  1:433820/1‑141 (MQ=12)
                                                                                                                     aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                         >  1:48080/1‑141 (MQ=17)
                                                                                                                     aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                         >  1:37730/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGACAATGCCCCaa                        >  1:353196/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTAAAGAATATGCCCCaa                        >  2:327455/1‑141 (MQ=17)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAAATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATACCCCaa                        >  2:147328/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGAGTAAACGTTCATCATACAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:32986/1‑141 (MQ=11)
                                                                                                                          ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCAtttt                    >  1:342/1‑141 (MQ=17)
                                                                                                                           gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCGCCAATTTta                   <  1:579097/141‑1 (MQ=255)
                                                                                                                             tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                 >  1:498063/1‑141 (MQ=255)
                                                                                                                              gTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACCTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                >  1:773460/1‑141 (MQ=25)
                                                                                                                               ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc               <  2:734400/141‑1 (MQ=25)
                                                                                                                                tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGCTCAAGAATATGCCCCAATTTTATATCa              >  1:515559/1‑141 (MQ=21)
                                                                                                                                 attaCTGTAGCTGTTGGTTACAATCTAAGAAATGCATTGTGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGGTCATCATTCTACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTGATATCaa             >  2:377069/4‑141 (MQ=11)
                                                                                                                                 aTCACTGTAGCTGTTGGATACGGTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATcca             >  2:560735/1‑139 (MQ=255)
                                                                                                                                 aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  1:598751/1‑141 (MQ=35)
                                                                                                                                   cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt           >  2:112966/1‑141 (MQ=25)
                                                                                                                                    aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt          >  2:689701/1‑141 (MQ=25)
                                                                                                                                    aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt          >  1:787755/1‑141 (MQ=255)
                                                                                                                                      tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttgg        >  2:519718/1‑139 (MQ=25)
                                                                                                                                          gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag    >  1:62686/1‑141 (MQ=255)
                                                                                                                                          gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag    >  1:656195/1‑141 (MQ=255)
                                                                                                                                          gCCGTTGGCTACTAATGAAGATATGCATTTAGCTATCGTGATATACCTGAAATATTAAGAGAACGTGTTGTAAACGTTCATCATTCACCGATTTACCGTTGGGTTCAATAATATGCCCCAAGTTTATATCAAATTTGgaag    <  2:275767/141‑1 (MQ=11)
                                                                                                                                           ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga   >  2:704268/1‑141 (MQ=255)
                                                                                                                                           ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga   >  2:639295/1‑141 (MQ=255)
                                                                                                                                            cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaataa  >  2:167615/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: