BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,444	C→T	52.6%	R36R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,444	0	C	T	52.6%	16.4 / inf	335	R36R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (77/81); new base T (86/89); total (165/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

Only 100 of 341 total aligned reads displayed.

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACT  >  CP000730/36305‑36582

  tttttGATAGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                         <  1:121549/140‑1 (MQ=11)

  aTTTTTATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTAGGGTATATTGTTGAACCCAACGGCAGTTAGTGCAATGATGAACGTTTACTGCACCTTCTCtt                                                                                                                                         <  2:757451/141‑1 (MQ=2)

   ttttTATGTAGATCCTATCAATACGCAATTTTTAATAAGCTTTTTTATTCTTTTTCTGCCAACTTTGATATATAACTGGAGAATATTCTTAATCCCAAGGGTAGACCGTTGAATGATGAACGTTTACCACACGTTCCCTTa                                                                                                                                        <  2:277345/141‑1 (MQ=2)

   ttttTATATACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                        <  2:786736/141‑1 (MQ=11)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                       <  2:316250/141‑1 (MQ=21)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                       <  2:450089/141‑1 (MQ=21)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                       <  1:665022/141‑1 (MQ=21)

    tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                       <  1:622828/141‑1 (MQ=255)

     ttgacttaagTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                                                      <  2:715030/132‑1 (MQ=14)

     ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                                                      <  1:418945/141‑1 (MQ=39)

      tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                                                     <  1:230854/141‑1 (MQ=21)

       gatatACGTCTCATCAATACGCCATTTGTAATACGTGATGGAAAGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATAGACGTTTACACCACGTTCTCTTAatat                                                                                                                                    >  1:778778/5‑141 (MQ=11)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                    >  1:730490/5‑141 (MQ=11)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                    >  1:711294/5‑141 (MQ=255)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                    >  2:125817/5‑141 (MQ=255)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                    >  2:293694/5‑141 (MQ=255)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGGTTACACCCCGTTCTCTTAatat                                                                                                                                    >  1:101883/5‑141 (MQ=11)

       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                    >  1:475201/1‑141 (MQ=40)

       gATATACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                    <  1:127110/141‑1 (MQ=11)

         tatACGTCTCATCAATTCGACATTTCTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                  <  1:212893/139‑1 (MQ=11)

          atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAGTAtttt                                                                                                                                 >  1:68947/2‑140 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                                >  2:62686/1‑141 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                <  1:603880/141‑1 (MQ=21)

            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                               >  1:536619/1‑141 (MQ=255)

            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                               >  2:394665/1‑141 (MQ=255)

            aCGTCTCATCAATACGCCAATTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                               >  1:433053/1‑141 (MQ=11)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                                              <  1:271097/141‑1 (MQ=18)

              ttctcAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                             <  1:370118/140‑1 (MQ=255)

              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                             <  2:605868/141‑1 (MQ=255)

                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACAGTAGACCGTTGAATGATGAACGTTTACACCACTTTCCCTTCATATTTCACataa                                                                                                                           >  2:2362/1‑140 (MQ=11)

                 tcatcaATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGGATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                                                          <  1:748994/141‑1 (MQ=255)

                 tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTCCTCTTAATATTTCAGatata                                                                                                                          >  1:638205/1‑141 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                         >  1:157679/1‑141 (MQ=255)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGTTGAACGTTTACACCAGGTTTTTTTAGTATTTCAGATATATc                                                                                                                        >  1:437433/1‑141 (MQ=11)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTCACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                        <  1:565006/141‑1 (MQ=11)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                                                        >  1:557806/1‑141 (MQ=25)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                                                        >  2:133580/1‑141 (MQ=21)

                   agcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                                                        <  1:217362/139‑1 (MQ=21)

                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                                                       >  1:644462/1‑141 (MQ=25)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTCAAACACACCGGTAGATCGTTGAATGATGCACGTTTCCACCACGTTTTCTTAATATTTCAGCTATATCag                                                                                                                      >  1:355155/1‑140 (MQ=11)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                      >  2:351828/1‑141 (MQ=255)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTGCTCTTAATATTTCAGATATATCAc                                                                                                                      >  2:116539/1‑141 (MQ=255)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                                                      >  2:769999/1‑141 (MQ=21)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                                                      >  1:91145/1‑141 (MQ=21)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                                                      <  1:287316/141‑1 (MQ=40)

                       aTACGCCTTTTTTAATAAGCTTTTTTATGCTTTTGCTTCCAAAATGGATATAAAATTGGAGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGTACGATTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                    <  2:245740/141‑1 (MQ=11)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                    <  2:105131/141‑1 (MQ=255)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                                                    <  2:379522/141‑1 (MQ=21)

                       aTACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                    >  1:506144/1‑141 (MQ=11)

                        tACGCCATTTGGTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                   <  2:309735/141‑1 (MQ=11)

                         accccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                  <  2:62144/138‑1 (MQ=255)

                         aCGCCATTTGTAATACGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATACCACGATa                                                                                                                  <  1:491481/141‑1 (MQ=11)

                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatatacgcata                                                                                                                  >  2:47576/1‑134 (MQ=11)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACTCCACGTTTCTTTAATTTTTTAGATATATCACGGTaa                                                                                                                 >  1:80076/1‑141 (MQ=11)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATTTTTCAGATATATCACGATag                                                                                                                 <  1:44753/141‑2 (MQ=11)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                 >  1:704896/1‑140 (MQ=255)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                 >  2:160180/1‑140 (MQ=255)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                 >  1:242193/1‑140 (MQ=255)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                 <  2:531112/141‑1 (MQ=21)

                          cGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                 <  2:482627/141‑2 (MQ=11)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATTACGCTAGCt                                                                                                               >  2:573776/1‑141 (MQ=11)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagct                                                                                                               >  2:718702/1‑138 (MQ=255)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACggtggctca                                                                                                             >  1:335842/1‑133 (MQ=11)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCAACGGTAGATCGATGAATGATGAAAGTTTACACAACGCTCTCTTAATAATTCagatagatgcagatagatca                                                                                                             >  2:22381/1‑129 (MQ=11)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                            <  2:633660/141‑1 (MQ=21)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                            <  1:257465/141‑1 (MQ=21)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                           >  1:225857/1‑141 (MQ=21)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTCCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                           <  2:55673/141‑1 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTTTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                          >  2:167028/1‑141 (MQ=255)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                          >  1:144420/1‑141 (MQ=25)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                          <  2:690831/141‑1 (MQ=18)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                          <  1:29944/141‑1 (MQ=39)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                          <  2:253603/141‑1 (MQ=40)

                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                         >  2:642231/1‑140 (MQ=18)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATAATCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                        >  1:389432/1‑139 (MQ=255)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGCTAGCTCAATgca                                                                                                        >  2:65944/1‑139 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                        >  1:198885/1‑139 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                        <  1:544595/141‑3 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                        >  2:423638/1‑139 (MQ=11)

                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                        >  2:206619/1‑139 (MQ=255)

                                    aataaGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTTAGATATATCACGATAACTCAAttcat                                                                                                       >  2:549609/1‑137 (MQ=11)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCATTGtaa                                                                                                       >  2:268896/1‑140 (MQ=255)

                                     ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACCCCACGCTCTCTTAATATTTCAGATATATCACGATagatatatgcata                                                                                                      >  1:410062/1‑129 (MQ=11)

                                               ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACAGCACGTTCACTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                            >  1:582588/1‑141 (MQ=25)

                                               ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                            >  2:337639/1‑141 (MQ=35)

                                                tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                           >  1:467483/1‑141 (MQ=35)

                                                 aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                          >  2:332236/1‑141 (MQ=35)

                                                             ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTTTATCTTAGATAGTAGCCAACGGCTAc                                                                              >  1:599114/1‑141 (MQ=21)

                                                              ttttttttATACAAAATTGGGGCATAATCTTGTACCCAACGGTAGACCGTTGAATGGTGAACGTTTACACCACTTTTTTTTATTATTTCAGATATATGATGATAACTCAATTTATATTTTAGATAGTAGCCAACgggtgaa                                                                             >  2:495916/5‑136 (MQ=11)

                                                                     gATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAAGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAAGGGCTACAGTgctca                                                                      >  1:521911/1‑137 (MQ=255)

                                                                                 gggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                          >  1:320265/1‑141 (MQ=32)

                                                                                  ggCCTATTCTTGAACCCAACGGTAGCCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATACCACGATAACTCAATGTATGTCTTAGATAGTAGCCACCGGCCACAGTGATAACCTCCCTGTTaca                                                         >  2:92202/1‑139 (MQ=11)

                                                                                              aaCCCATCCGTAGATTGTTTAATGATGAACGTTTACACGACGTCCTCCTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGTCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                             <  2:537633/141‑1 (MQ=11)

                                                                                                  cAACCGTAGACCCTTGAATAATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                         <  2:468732/141‑1 (MQ=12)

                                                                                                          gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAt                                 >  1:303520/1‑141 (MQ=32)

                                                                                                                              ttACACCACGTTCCTTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt             <  2:610387/141‑1 (MQ=17)

                                                                                                                                  cccccGTTCTCTTAGTATTTCAAATATACCACGATAGCTCAATGAATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaaa         <  1:266322/137‑1 (MQ=1)

                                                                                                                                   ccacGTTCCCTTAATATTGCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt        <  1:23867/141‑1 (MQ=17)

                                                                                                                                      cGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATtat     >  2:18857/1‑141 (MQ=32)

                                                                                                                                         tCCCTTAACATATCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACt  <  2:507431/141‑1 (MQ=17)

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACT  >  CP000730/36305‑36582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`