Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,704,755 C→T 11.9% G363S (GGT→AGT)  USA300HOU_2558 ← glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,704,7550CT11.9% 193.5 / 13.4 83G363S (GGT→AGT) USA300HOU_2558glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (39/32);  new base T (4/6);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TCTTCATGATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTGTAGTATAGCTCA  >  CP000730/2704620‑2704892
                                                                                                                                       |                                                                                                                                         
tcttcATGATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAAc                                                                                                                                      <  2:160752/141‑1 (MQ=255)
 cttcATGATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACg                                                                                                                                     >  1:44482/1‑141 (MQ=255)
  ttcATGATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGa                                                                                                                                    >  1:544470/1‑141 (MQ=255)
       gATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACTATAACGATGCgt                                                                                                                               >  1:583860/1‑141 (MQ=255)
            tATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCTTTACCCTGATTTATTGTTCTTTAGATATAGTATATTTACGACCTTTCCATTCAACTACACCATAACGATGCgtttgtt                                                                                                                          >  1:61936/1‑141 (MQ=255)
            tATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCgtttgtt                                                                                                                          <  2:365573/141‑1 (MQ=255)
             ttGACAATATTTAAAATTCATATCCTTTATTGTCATCCTAGTCCTCCAATAACGAACAGCCATATCCTTGATTTATTGTTCTGTAAATATATAAGATGGACTATCTTTCCATTCAACTACACCATAACGATGCgtttgttt                                                                                                                         <  2:458678/140‑1 (MQ=255)
               gACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTcc                                                                                                                       >  1:427698/1‑141 (MQ=255)
                ataaTATTAAAAATCCACATCCATCATTGTCATACTAGTCCGCCTATATTTAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGATCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCa                                                                                                                      <  1:540788/139‑1 (MQ=255)
                aCAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAGTGCCATTACCTTGATTTATTGTTCTTTAGCTATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTcaa                                                                                                                      >  2:451317/1‑139 (MQ=255)
                aCAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCa                                                                                                                      >  2:783624/1‑141 (MQ=255)
                aCAATATTTAAAATTCATATCCATCATTGCCATACAATTTCTCCTATATTGAAATGACATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAAACCTGCCTTGGTTTCCa                                                                                                                      >  1:30469/1‑141 (MQ=255)
                 aaaTATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCaa                                                                                                                     <  2:474669/140‑1 (MQ=255)
                  aaTATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACATTTCCATTCAACTACACCATGACGATGCGTTTGTTTACAAg                                                                                                                    >  1:525448/1‑141 (MQ=255)
                      tttAAAATTCATATCCATCATTGTCATACTAGGCCTCCTATATTGAAATGCCATTCCCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTg                                                                                                                <  2:730435/141‑1 (MQ=255)
                        tAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAAATTggt                                                                                                              >  2:573414/1‑140 (MQ=255)
                           aaTTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGa                                                                                                           >  2:392762/1‑141 (MQ=255)
                           aaTGCATATCCATCTTTGTCACACTAGTCCTCCTATATGGAAATGTCATTACCTTGATTTAGTGATCTTGTGATATAGAATATTGGCTACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGa                                                                                                           <  1:777257/141‑1 (MQ=255)
                                atatCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAAtt                                                                                                      <  2:602355/141‑1 (MQ=255)
                                aaatCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTGGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAAtt                                                                                                      <  2:470681/139‑1 (MQ=255)
                                   tCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTGCCAAGATTGGTTGaaaaggttg                                                                                                   >  1:178372/1‑136 (MQ=255)
                                             tCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACa                                                                                         >  2:474037/1‑141 (MQ=255)
                                               aTACTTGTCCGCCTATATTGGAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACata                                                                                       <  1:197494/141‑1 (MQ=255)
                                                 aCTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTGTAGTAAAAAACataaa                                                                                     <  1:393195/141‑1 (MQ=255)
                                                   tAGTCCTCCTATATGGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACataaata                                                                                   <  1:131992/141‑1 (MQ=255)
                                                     gTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTCGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGAGGAAAATTTTAGTAAAAAACATAAATAAc                                                                                 >  1:446121/1‑141 (MQ=255)
                                                     gTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAAc                                                                                 <  1:354519/141‑1 (MQ=255)
                                                     gTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAAc                                                                                 <  1:526649/141‑1 (MQ=255)
                                                      tcctccTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAcacacaggtaaac                                                                                >  1:731526/1‑132 (MQ=255)
                                                      tcctccTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACa                                                                                <  2:454934/141‑1 (MQ=255)
                                                         tcctATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATg                                                                             >  2:733598/1‑141 (MQ=255)
                                                          cctATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATGGAATATTGACGACCTTTCCATTCAACTACCCAATAAAGATGCGTTGGTTTCCAAGATTGGATGAAGATTTTAGAAAAAAACATAAATAACAATgg                                                                            >  2:417997/1‑141 (MQ=255)
                                                          cctATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGACAAAGAATATTGATCAAATTTTCATTTAAATTTATATTAAAGATGAGATTATTTCAAAATTATTAATATAATTTTAGTAAAAAACATAAATAACAATgg                                                                            >  2:458135/1‑141 (MQ=255)
                                                            tataTTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAACTTTTAGTAAAAAACATAAATAACAATGGAt                                                                          >  2:91526/1‑141 (MQ=255)
                                                            tataTTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGAt                                                                          <  1:199584/141‑1 (MQ=255)
                                                            tataTTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGAt                                                                          <  1:290733/141‑1 (MQ=255)
                                                            tataTTGAAATGCCATTACCTTGAGTTATTGTTCTTTAGACATAGAAGATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGAt                                                                          <  1:608998/141‑1 (MQ=255)
                                                              tatTGAAATGCCTTTACCTTGATTTCTTGTTCTTTACATAGAGAAGATTGACGACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGa                                                                        <  1:409770/141‑1 (MQ=255)
                                                              tatTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCAAAGATTGGATAAAAATTTTAGTAAAAAACATAAATAAAAATGGagta                                                                        >  2:207777/1‑138 (MQ=255)
                                                                    aaTGCCATTACCTTGTCTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATAcc                                                                  >  1:394767/1‑141 (MQ=255)
                                                                        cgaTTACCTTGGTTTATTGTTCTTTGGATATAGAATATTGAGGACCTTTCTATTCAACTTGCGCGTAACGATGCGTTTGTTTCCAAGTTTGGATGATAATTTTGGTAAAAAACTTTAATAACAGTGGTTGACATACCAttt                                                              >  2:639538/3‑140 (MQ=255)
                                                                        ccATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATta                                                              >  1:591042/1‑141 (MQ=255)
                                                                         cATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATtaa                                                             >  2:245796/1‑141 (MQ=255)
                                                                          aTTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATaaaa                                                            >  1:292664/1‑140 (MQ=255)
                                                                             aCCTTGATTTATTGTTCCTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATaaa                                                         <  1:386492/141‑1 (MQ=255)
                                                                              ccTTGAATTATTGTTCTTTAGATATAGAATATTGACGACCATTCCCTTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGAAATACCATTAATAAAt                                                        >  2:584728/1‑141 (MQ=255)
                                                                                      tATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAAt                                                <  2:713930/141‑1 (MQ=255)
                                                                                         tctgctatAGATATATAAGATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTgg                                             <  2:195400/134‑1 (MQ=255)
                                                                                         tGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAAAAAATTgg                                             >  2:129540/1‑141 (MQ=255)
                                                                                         tGTTCGTTAGATATAGAATATTGACGACCTTTCCATTCAACTAGGCCTAAACGATTCGTCTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTgg                                             <  2:44482/141‑1 (MQ=255)
                                                                                           ttCTTTAGATATAGAATATTGACGACCTTTCCATTCCACTACACTATAACGATGCGTTTGTTTCCAAGATAGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGcc                                           >  1:708610/1‑141 (MQ=255)
                                                                                           ttCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGAAATACCATTAATAAATTAGAAAATTGGcc                                           >  2:647841/1‑141 (MQ=255)
                                                                                             cTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAACACCCGATGTAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGa                                         >  2:706662/1‑141 (MQ=255)
                                                                                              tttAGATATAGAATATTGACGACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGAc                                        >  1:700360/1‑141 (MQ=255)
                                                                                              tttAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGAc                                        <  2:385584/141‑1 (MQ=255)
                                                                                               ttAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGAcc                                       <  1:370536/141‑1 (MQ=255)
                                                                                                tAGATATAGAATATTGACGACCTTTCCATTCAACTACGCCATAACGATGCGTTTGTTTACAAGATTGGATGAAAATTTTAGAAAAAAACATAAATAACAATGAATGACGTACCATTAATAAATTAGAAAATTGGCCGAcct                                      >  2:358025/1‑140 (MQ=255)
                                                                                                 aGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCt                                     <  1:575181/141‑1 (MQ=255)
                                                                                                  gATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCGGACCCtt                                    >  2:256468/1‑141 (MQ=255)
                                                                                                   atatAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTc                                   >  2:352116/1‑141 (MQ=255)
                                                                                                          ataaTGACGACCTATCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAg                            <  1:307294/141‑1 (MQ=255)
                                                                                                           tatTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGa                           >  1:98301/1‑141 (MQ=255)
                                                                                                              tGACGACCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataa                        <  1:217578/141‑1 (MQ=255)
                                                                                                              tGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataa                        >  1:548626/1‑141 (MQ=255)
                                                                                                              tGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataa                        >  1:220738/1‑141 (MQ=255)
                                                                                                               gacgacCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaa                       <  2:583860/141‑1 (MQ=255)
                                                                                                               gacgacCTTTCCATTCAACTACACTATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCAATGCAGataaa                       >  1:666126/1‑141 (MQ=255)
                                                                                                               gacgacCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaa                       >  1:173984/1‑141 (MQ=255)
                                                                                                               gacgacCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaa                       <  1:745965/141‑1 (MQ=255)
                                                                                                                acgacCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaat                      <  1:184264/141‑1 (MQ=255)
                                                                                                                acgacCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCAACCCTTCGATGCAGataaat                      >  2:325948/1‑141 (MQ=255)
                                                                                                                 cgacCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACACTTCGATGCAGataaata                     >  1:659568/1‑141 (MQ=255)
                                                                                                                 cgacCTTTCCATTCAACTAAACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaata                     <  2:244146/141‑1 (MQ=255)
                                                                                                                 cgacCTTTCCAATCAACTACACCATAACGATGTGTTTGTTTCCATGATCTTATTAAAATAATAGAAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGataaata                     <  2:464830/141‑1 (MQ=255)
                                                                                                                        tCCATTCAACTACACCATAACGGAGCGTTTGTTTCCAAGATTGGATGAAAATTTTGGTAAAAAACATAAATAACAATGGGTGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATtgtg              >  2:317125/1‑141 (MQ=255)
                                                                                                                          cATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTgta            <  2:61936/141‑1 (MQ=255)
                                                                                                                          cATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAAGGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTgta            <  2:178372/141‑1 (MQ=255)
                                                                                                                            ttCAACTACACCATAACGATGCGGTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATAACATTAATAAAGAAGAAAATTGGCCGACCCTTCGATGCAGATAACAAAATAGTgtatt          >  2:180586/1‑139 (MQ=255)
                                                                                                                              cAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTGTAGtat        <  2:544470/141‑1 (MQ=255)
                                                                                                                              cAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTGTAGtat        >  2:339297/1‑141 (MQ=255)
                                                                                                                                 cTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATGAATTGTGTAGTATAGc     <  1:392762/141‑1 (MQ=255)
                                                                                                                                 atACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTGTAGTATAGc     <  2:525448/140‑1 (MQ=255)
                                                                                                                                    cacCATAACGATGCGTTTGTTTCCAAGATTGGTGGAAAAATTTAGGAAAAAACATAAATAACAATGGATAACATACCAGTAATCAATTAGAAAAATGGCCGACCCTTCGATCCAGATAAATACATGGTGAAGtatatccca  >  2:278660/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TCTTCATGATTTTATGACAATATTTAAAATTCATATCCATCATTGTCATACTAGTCCTCCTATATTGAAATGCCATTACCTTGATTTATTGTTCTTTAGATATAGAATATTGACGACCTTTCCATTCAACTACACCATAACGATGCGTTTGTTTCCAAGATTGGATGAAAATTTTAGTAAAAAACATAAATAACAATGGATGACATACCATTAATAAATTAGAAAATTGGCCGACCCTTCGATGCAGATAAATAAATTGTGTAGTATAGCTCA  >  CP000730/2704620‑2704892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑