Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,512 A→G 30.5% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5120AG30.5% 35.7 / 15.9 31S194S (TCA→TCGUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (8/13);  new base G (4/5);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT  >  CP000730/111372‑111644
                                                                                                                                            |                                                                                                                                    
tCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCAAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCa                                                                                                                                      >  1:131885/1‑141 (MQ=255)
  tACTGAGGAAGTTAAAGTAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                                    <  2:226946/141‑1 (MQ=255)
   aCTGAGGAAGTTAAGGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTGTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGAGCCGAGTTATTCGGCt                                                                                                                                   >  1:163142/1‑141 (MQ=21)
   aCTGAGGAAGGTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCt                                                                                                                                   <  1:79310/141‑1 (MQ=255)
   aCTGAGAAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCGGCt                                                                                                                                   >  2:156994/1‑141 (MQ=255)
       aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                               >  2:80448/1‑141 (MQ=255)
                aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAAAGGTGCCGAGTTATTCAGCTAAATATCAATTaa                                                                                                                      >  2:25091/1‑141 (MQ=255)
                    agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                  <  1:177008/141‑1 (MQ=255)
                     aaaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTATTTGCGCGATGTTGCAGTTTGAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                 <  2:212267/140‑1 (MQ=18)
                          cATAAAAAAAGAAATCGAAATCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                            <  1:133086/141‑1 (MQ=255)
                                 aaaGAAATCGAAAACTTTAAGTTTTTTCCGCAATATAGCAATTTTAAATATTTAATTAATTATAAAGATGGAGATATATCATATAATCCGGAGGTGCCGAGTAATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                     >  2:157457/1‑141 (MQ=17)
                                   aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                   <  1:40044/141‑1 (MQ=255)
                                    aaaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTa                                                                                                  <  2:156799/140‑1 (MQ=255)
                                     aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGGGGAATTCGGCTAAATATAGATTAACTAATGATGATGATAATGTaa                                                                                                 >  1:83140/1‑141 (MQ=18)
                                     aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                 <  1:111707/141‑1 (MQ=255)
                                     aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                 <  1:111718/141‑1 (MQ=255)
                                      aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                <  2:146506/141‑1 (MQ=255)
                                      aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                <  2:106297/141‑1 (MQ=255)
                                      aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATGTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                <  1:116287/141‑1 (MQ=255)
                                            aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                                          >  1:205994/1‑141 (MQ=255)
                                              aCTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                        <  2:43575/141‑1 (MQ=255)
                                               cTTTAAGTTTTTTGCGAAATATTGGAATTTAAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                       <  1:208672/141‑1 (MQ=17)
                                                     gTTTTTTGCGCAATATGGCAATTTTAAAGAGTTAATCAACTATAAAGATGGAGATATATCATATAATCCAGAGGTGGCGAGTTATTCTGCTAAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTCCGCaaacg                                                                                 <  2:221350/141‑3 (MQ=17)
                                                      ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                <  1:202479/141‑1 (MQ=255)
                                                               cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAGCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                       >  1:72262/1‑141 (MQ=255)
                                                                  tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGAGGATTATAATGTAAAACAATTACGTAAAAAATATGACATACca                                                                    >  2:83168/1‑141 (MQ=255)
                                                                                 aaTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCTATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                     <  1:245744/140‑1 (MQ=255)
                                                                                                                   ataATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                   <  1:73003/141‑1 (MQ=255)
                                                                                                                          agagGTGCCGAGTTATTCACCTCGCTTACAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAACAGGTtcatc            >  1:183734/1‑141 (MQ=17)
                                                                                                                            agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCCTCAg          >  2:168183/1‑141 (MQ=255)
                                                                                                                                 ccGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa     >  2:162457/1‑141 (MQ=255)
                                                                                                                                    ggtgatgcAGCTCAATATCAAGTGACTAATGATGATTATAATGTAAAACAATTACGTAAAAGGTATGACATACCAACAAATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat  <  1:174106/134‑1 (MQ=14)
                                                                                                                                            |                                                                                                                                    
TCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT  >  CP000730/111372‑111644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: