Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,279,7050GA100.0% 87.2 / NA 32intergenic (+1373/+6659)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTT  >  CP000730/2279575‑2279836
                                                                                                                                  |                                                                                                                                   
gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGTACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                           <  2:163192/141‑1 (MQ=255)
           aTTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg                                                                                                                >  1:229168/1‑141 (MQ=255)
              cTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTTCTTATGTTACTGTTGTTTCAACTGTTGCCGCAt                                                                                                             >  1:54098/1‑141 (MQ=255)
                aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                           >  1:147810/1‑141 (MQ=255)
                     tGGTTTACTAGTTACATCGGTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatt                                                                                                      >  1:162812/1‑141 (MQ=255)
                       ggttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGGTGTTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtattat                                                                                                    <  1:46114/139‑1 (MQ=255)
                        tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTCGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTattatt                                                                                                   >  2:40332/1‑141 (MQ=255)
                             tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtt                                                                                              >  2:14564/1‑140 (MQ=255)
                                   cATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCGAACCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTATTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGCGTTGTCg                                                                                        <  1:8790/141‑1 (MQ=255)
                                            ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                               <  1:200807/141‑1 (MQ=255)
                                                    ggACTGTTTGTTGATGCATCTACACTAGAATTGTTACTAGGTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTGTTTGGTGGTGTCGGAGAAGCGTGTGCTTTg                                                                       >  2:98961/1‑141 (MQ=255)
                                                      accgtttgttGATGCATTTACACTAGAAATGAAATCATATGACAGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                     <  1:232536/138‑1 (MQ=255)
                                                      aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                     <  1:86279/141‑1 (MQ=255)
                                                        tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGGTTTGCCAt                                                                   >  2:18209/1‑141 (MQ=255)
                                                              ttgATGCATCTACACTGGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTg                                                             >  1:124872/1‑141 (MQ=255)
                                                                    gatttACACTATAATGGTTCTTAGCTTGCGGTTTATCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                       <  1:23723/137‑1 (MQ=255)
                                                                    gaTCTACACTAGGATTGTTGTTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                       <  1:202227/140‑1 (MQ=255)
                                                                           acTCGAAGTGTTATGTGCGTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGGGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATAc                                                <  2:154288/141‑1 (MQ=255)
                                                                           acTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATAc                                                <  2:18574/141‑1 (MQ=255)
                                                                             tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTGGTCTCACCTGTTGCCGCATCACTATGATTTGGTGTTGTCGGCGCAGCGTCTGCTTTGCCCTTAGCGGTCGTCTAAGATACGt                                              >  2:56637/1‑141 (MQ=255)
                                                                              aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                             >  2:32965/1‑141 (MQ=255)
                                                                                 aTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                          <  1:192170/141‑1 (MQ=255)
                                                                                      tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                     >  2:117445/1‑141 (MQ=255)
                                                                                         taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                  <  2:78095/141‑1 (MQ=255)
                                                                                          aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCTGATACGTTAGGTTGTCCAGt                                 <  1:152342/141‑1 (MQ=255)
                                                                                              tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGGTGTCGAGTAttt                             >  1:50547/1‑141 (MQ=255)
                                                                                               tcGGTTTATCTTTTGTATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                            <  2:182342/140‑1 (MQ=255)
                                                                                               gCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                            <  2:184429/141‑1 (MQ=255)
                                                                                                              catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAg             >  1:90790/1‑141 (MQ=255)
                                                                                                                   tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTGCTGGTGTTGCATTAGCAttt        >  1:165181/1‑141 (MQ=255)
                                                                                                                      tttGCTGATGTTACTGTTGTTTCACCTTTTTCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa     <  2:162812/140‑1 (MQ=255)
                                                                                                                         gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAttt  <  1:84541/141‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTT  >  CP000730/2279575‑2279836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: