Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,867 G→T 63.8% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8670GT63.8% 147.3 / 190.3 191intergenic (‑950/‑218)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base T (58/62);  total (94/97)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 192 total aligned reads displayed.

TGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929727‑1930005
                                                                                                                                                |                                                                                                                                            
tgtAGACGATTCAAGCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCTCTCCGGCGTCAATTTAGACGCAGAGGGGAGGTG‑TA‑TAA‑g‑g                                                                                                                                              >  1:504572/1‑141 (MQ=255)
          tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAt                                                                                                                                  <  2:820416/141‑1 (MQ=255)
           cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAtt                                                                                                                                 >  1:828033/1‑141 (MQ=255)
            aaTCTATCAGCTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAttt                                                                                                                                <  1:607812/141‑1 (MQ=255)
             aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGTGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAtttt                                                                                                                               <  1:582428/141‑1 (MQ=255)
                         gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCAcatcat                                                                                                                   <  1:16187/141‑1 (MQ=255)
                          aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTGTATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGGTCACATCATa                                                                                                                  >  1:196502/1‑141 (MQ=255)
                                  aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                                          <  2:283593/141‑1 (MQ=255)
                                        tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                                                                                    >  2:459763/1‑141 (MQ=255)
                                           atcggaattagatatgCATGTACATTCACAGATGCTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                <  1:458780/127‑1 (MQ=255)
                                           atcggaagtatatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTT‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                <  1:435092/127‑1 (MQ=255)
                                           atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                <  1:242005/127‑1 (MQ=255)
                                           atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                <  2:616381/127‑1 (MQ=255)
                                           atcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                <  1:240846/127‑1 (MQ=255)
                                            tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                               >  1:112339/14‑141 (MQ=255)
                                            tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                               >  2:567492/14‑141 (MQ=255)
                                            tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCGT‑TAC‑T‑T‑T‑CCTCATCGCTTTCGTTCACATCATAGCACCAGTCATCAGCGGCt                                                                                               >  1:616651/14‑141 (MQ=255)
                                            tcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCt                                                                                               >  2:199155/14‑141 (MQ=255)
                                              ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                             <  2:336666/130‑1 (MQ=255)
                                                aagtagatatgCTTGTTTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGc                                                                                           <  1:356595/132‑1 (MQ=255)
                                                aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                           <  2:638240/132‑1 (MQ=255)
                                                  gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGGCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GCTAATCATTTTCGTTCACATGATAGCACCAGTCATCATTGGCTGTGctg                                                                                         >  1:515352/8‑139 (MQ=255)
                                                    gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATATTACGAGGCATCAGTGGCTGTGCCAt                                                                                        <  2:646762/141‑1 (MQ=255)
                                                    gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                        <  1:691294/141‑1 (MQ=255)
                                                    agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                       >  1:559027/6‑137 (MQ=255)
                                                    agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                       <  2:245269/136‑5 (MQ=255)
                                                     gatatgCTTGTATTTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                      >  1:572585/5‑136 (MQ=255)
                                                     gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                      <  1:882442/137‑6 (MQ=255)
                                                       tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                     <  2:779727/141‑1 (MQ=255)
                                                       tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                     >  1:122779/1‑141 (MQ=255)
                                                       tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                    >  1:208309/3‑141 (MQ=255)
                                                       tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACAAGTCATCATTGGCTGTGCTGTTGCg                                                                                    >  2:226741/3‑141 (MQ=255)
                                                       gatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTGTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTCAGCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                    <  2:56404/139‑1 (MQ=255)
                                                        atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   <  1:637536/140‑1 (MQ=255)
                                                           cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                <  1:110514/141‑4 (MQ=255)
                                                            ttGTATATTCACAGAAGGTATAATATGAGAGTCGCCTAGCTCTCAGGCTTCAAGTT‑‑ACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGGCAGCAGTGGCTGTGCTGTTGCGtagta                                                                               <  2:166106/141‑5 (MQ=255)
                                                             tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGttttat                                                                              <  2:631480/141‑3 (MQ=255)
                                                               tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  1:194973/141‑8 (MQ=255)
                                                               tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  2:307152/141‑5 (MQ=255)
                                                                atatTTGCCGATGATATAATAGTAGAGTGGCATATCTCTCAGGCGTCAAGTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCCATTGCGTTTTTTTc                                                                            >  1:264965/1‑141 (MQ=255)
                                                                   ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCAATCACATCATAGCACCAGTCATCACTGTCAGTGCTGTTGCGTATTATACTTAt                                                                        >  2:145154/7‑141 (MQ=255)
                                                                     cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                      >  2:115255/5‑141 (MQ=255)
                                                                       cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                    <  2:104301/139‑1 (MQ=255)
                                                                       cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                    <  2:914863/139‑1 (MQ=255)
                                                                        cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                    <  1:723492/141‑1 (MQ=255)
                                                                        agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCCATT‑‑GACGAAGGGAGGAGGTG‑CA‑TTT‑CTT‑T‑GCTAGTCATTTTCGTTGACATCGTAGGACCGGTCGTCGGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                   <  2:667089/140‑1 (MQ=255)
                                                                         gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                  <  2:559027/141‑2 (MQ=255)
                                                                           tgatATAATAGTAGAGTCGCCTATCTCTCAGGAGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                 >  1:653723/1‑141 (MQ=255)
                                                                            ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:104876/3‑141 (MQ=255)
                                                                            ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:609994/3‑141 (MQ=255)
                                                                            ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  2:404668/3‑141 (MQ=255)
                                                                            ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATGATACTTATTGGCTTAGt                                                               <  1:219423/139‑1 (MQ=255)
                                                                            ggtacaataatAGAGTCGCCTATCTCTCAGCCGTCAATT‑‑GACGAAGAGAGGAGATG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAga                                                               >  2:554136/6‑140 (MQ=255)
                                                                              tataATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                              >  1:325535/1‑141 (MQ=255)
                                                                              tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                             >  2:133234/1‑140 (MQ=255)
                                                                              tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                             >  2:731600/1‑140 (MQ=255)
                                                                              tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTaa                                                             >  2:698714/1‑140 (MQ=255)
                                                                              tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                             >  1:255172/1‑140 (MQ=255)
                                                                               ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                            >  1:777417/1‑139 (MQ=255)
                                                                               ataataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGGTAAGtca                                                             >  2:578842/1‑139 (MQ=255)
                                                                                taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                            >  2:65728/1‑141 (MQ=255)
                                                                                  ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         >  1:291904/5‑141 (MQ=255)
                                                                                  ataataGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑TCGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         <  2:601457/141‑1 (MQ=255)
                                                                                   taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAAGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                        <  2:852428/138‑1 (MQ=255)
                                                                                    aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑GTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                       <  2:671873/139‑1 (MQ=255)
                                                                                       agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                     <  1:846460/141‑1 (MQ=255)
                                                                                       agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                    <  1:790465/141‑4 (MQ=255)
                                                                                        gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                    >  1:316406/1‑141 (MQ=255)
                                                                                         agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                   >  1:521234/1‑141 (MQ=255)
                                                                                         agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  <  1:677652/141‑6 (MQ=255)
                                                                                          gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCGTTTGCGTTTTATACTTATTGGCTTAGTAAACGCaacaaata                                                 >  1:189030/1‑135 (MQ=255)
                                                                                          gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑AATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACACaacaaata                                                 >  2:597656/1‑135 (MQ=255)
                                                                                             gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                               >  2:432488/1‑141 (MQ=255)
                                                                                             gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑AA‑TAA‑G‑G‑T‑GATGGTTATTGTCTTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                               <  2:277341/141‑1 (MQ=255)
                                                                                             gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              <  2:623208/141‑10 (MQ=255)
                                                                                             gCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              <  1:878105/141‑10 (MQ=255)
                                                                                                tATCTCTCAGGCTTCAATT‑‑GGCGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTTGTTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                           <  2:365251/141‑13 (MQ=255)
                                                                                                tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                           >  2:530364/1‑129 (MQ=255)
                                                                                                tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                           <  2:59483/141‑13 (MQ=255)
                                                                                                tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCGGTTGCGTATTATACTTATTGGCTGAGTAGACACaacaaataattaaa                                           >  1:141676/1‑129 (MQ=255)
                                                                                                tATCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                           <  1:23842/141‑13 (MQ=255)
                                                                                                 ttctctcAGGCGTCAATT‑‑GACGGAGAGAGGAGTTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          <  1:98123/140‑14 (MQ=255)
                                                                                                 aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          <  1:819367/141‑14 (MQ=255)
                                                                                                 aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          >  1:606306/1‑128 (MQ=255)
                                                                                                 aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaatcattaatg                                          >  1:59342/1‑128 (MQ=255)
                                                                                                  tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCTTTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTg                                          >  1:802774/1‑141 (MQ=255)
                                                                                                  tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattattag                                         <  2:790341/141‑15 (MQ=255)
                                                                                                  tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                         <  1:728110/141‑15 (MQ=255)
                                                                                                  tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                         >  2:808231/1‑127 (MQ=255)
                                                                                                  tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAATTATTGGCTGAGTAAACCCaacaactaattaatag                                         >  1:790687/1‑127 (MQ=255)
                                                                                                  tccctcggacGTCAGTTTAAGCGAAGAGAGGAGGCG‑TA‑TAA‑G‑G‑T‑GATGCGGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTGAGGGGTAAGGAGACGCAAGACAAAATAGGTg                                          >  2:873909/10‑141 (MQ=255)
                                                                                                   ctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGATCACGCGGGTTCACGTAATAGTCCCAGGCATCAGTGGCTGTGCCATTGCGTTTTTTGCTGATTTGCTAAGTAGACGCAATACAAAATAGGTGa                                         >  1:901647/1‑141 (MQ=255)
                                                                                                                         cAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa                   <  2:714883/141‑1 (MQ=255)
                                                                                                                         cAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa                   <  1:368091/141‑1 (MQ=255)
                                                                                                                                   gtg‑tA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt         <  1:753867/141‑1 (MQ=255)
                                                                                                                                    tg‑tA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAATTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa        >  2:736959/1‑141 (MQ=255)
                                                                                                                                       ta‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAcc      <  2:338221/141‑1 (MQ=255)
                                                                                                                                        a‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCg     >  1:84796/1‑141 (MQ=255)
                                                                                                                                            a‑G‑G‑T‑GATGCTTATTTACGTTCACATCTTAGCACCTGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGTCGCAATACAAAATGGGTGACATATAGCCGCACCAATATAAATCCCCTCACTACCGCaa  <  1:551202/141‑1 (MQ=255)
                                                                                                                                            a‑G‑G‑T‑CATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCCACTGCGTTTTTTTCTTATTGGCTACGTAGACGCAATACAACATCGGTCACATATAGCCGCACCAATACAAACCCCCTCACTACCGCaa  <  2:477213/141‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
TGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929727‑1930005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑