Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 55.5% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA55.5% 96.9 / inf 516intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (114/113);  new base A (149/132);  total (266/250)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-02

Only 100 of 520 total aligned reads displayed.

GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTG  >  CP000730/76213‑76493
                                                                                                                                            |                                                                                                                                             
   tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                                                            <  1:49419/141‑1 (MQ=255)
     aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                                                          >  1:289995/1‑141 (MQ=12)
     aaCTATTTCAGATATAAACAATTTAACAAGGATGTCATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                                                          <  1:28420/141‑1 (MQ=17)
      aCTATTTCAGATGTAAACAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAGCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                         <  2:171507/141‑1 (MQ=11)
       cgagttCAGATATAAACAATTTAACAAGGCTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCg                                                                                                                                        <  2:74007/137‑1 (MQ=11)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGAACTACCGTTg                                                                                                                                     >  2:127769/1‑141 (MQ=255)
          tctCAGACACAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                     <  1:75639/139‑1 (MQ=11)
           ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTCAGGGACCTTATTGGAAGCGTTCATCATTCAACGGTCTAGCGTTgg                                                                                                                                    >  1:702575/1‑141 (MQ=11)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                   <  1:887617/141‑1 (MQ=255)
             cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCTATGGGt                                                                                                                                  >  2:89018/1‑141 (MQ=11)
             cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTAGCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                                                  >  1:128700/1‑141 (MQ=11)
                atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                                                                               <  2:67415/141‑1 (MQ=12)
                  ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                             <  1:42570/141‑1 (MQ=17)
                  ataAACAATCTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                             <  1:149696/141‑1 (MQ=11)
                   taAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                            <  1:352440/141‑1 (MQ=255)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                            >  1:839552/1‑141 (MQ=12)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTAGGTTCAAGa                                                                                                                            >  1:595185/1‑141 (MQ=255)
                   aaaaCAATTTAAAAAGGGCGTTATCGCTGGAGCTGGTGGCTCCTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                                                            <  2:269755/140‑1 (MQ=11)
                    aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                                                                                           >  1:726540/1‑141 (MQ=12)
                        aaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                                                       >  1:312540/1‑141 (MQ=17)
                        aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTACCGTTGGGTTAAAGAatatt                                                                                                                       >  1:589041/1‑140 (MQ=11)
                           ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                                                    >  2:290905/1‑141 (MQ=17)
                           ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                                                                                    >  2:610407/1‑141 (MQ=17)
                           ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                                                                                    >  2:864113/1‑141 (MQ=12)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccccca                                                                                                                 >  2:772400/1‑139 (MQ=17)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                 >  2:901111/1‑141 (MQ=17)
                              aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                 >  2:638203/1‑141 (MQ=17)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                 >  1:527751/1‑141 (MQ=12)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                 >  1:909257/1‑141 (MQ=255)
                               cAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                                                >  1:695715/1‑141 (MQ=17)
                                   gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                            >  1:376758/1‑141 (MQ=255)
                                    aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTTTGTTGATATATCTGAACTCTTAGGTGAACGTGGTGTTTTCTTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                           <  1:435773/141‑1 (MQ=11)
                                    aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAGTTtat                                                                                                           >  2:662988/1‑141 (MQ=255)
                                     tGTTATCACCGTAGCCGTTGGCTACCATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAGTCtata                                                                                                          <  2:788946/141‑1 (MQ=11)
                                      tttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                         <  2:711420/140‑1 (MQ=25)
                                      tttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                         <  2:182926/140‑1 (MQ=255)
                                      gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                         >  1:189132/1‑141 (MQ=255)
                                      gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                         <  1:782915/141‑1 (MQ=255)
                                      gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                         >  2:841748/1‑141 (MQ=255)
                                       ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATCACAGTTTTTGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                                                        <  1:663042/141‑1 (MQ=11)
                                        tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAGGAATATGCCCCAATTTTATATCa                                                                                                       >  1:343759/1‑141 (MQ=25)
                                        tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                       >  1:628876/1‑141 (MQ=25)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                       <  2:114229/141‑1 (MQ=255)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                       <  2:751333/141‑1 (MQ=255)
                                        gaTCACTGCAGCAGTGGGCTAGTATCTAAGATATGCATTCAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                       <  1:866322/140‑1 (MQ=11)
                                         aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTGGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                      >  1:389382/1‑141 (MQ=255)
                                          tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                     >  1:111334/1‑141 (MQ=255)
                                           cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTCCACCGCTGAACGAGCTACCGTGGGGTTCAAGAATATGCCCCAACTTTATATCAAAt                                                                                                    >  2:877000/1‑141 (MQ=11)
                                            aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt                                                                                                   <  1:135819/141‑1 (MQ=255)
                                              tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                                 <  1:306307/141‑1 (MQ=25)
                                                tAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                               <  2:312091/141‑1 (MQ=25)
                                                tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGTTATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                               <  1:852659/141‑1 (MQ=255)
                                                  tcgagtGGCTACTATCTAGTATATTGAGTGTTCTAGCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCAGCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATAGCAAATTTGgaag                                                                                             <  2:227211/136‑1 (MQ=11)
                                                  gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTCGgaag                                                                                             >  1:404980/4‑141 (MQ=25)
                                                  gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                             >  2:810823/1‑141 (MQ=255)
                                                   ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                                                            >  1:832423/1‑141 (MQ=255)
                                                    cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                           >  1:173216/1‑141 (MQ=255)
                                                     gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATGTTATATCAAATTTGGAAGaaa                                                                                          >  1:207636/1‑141 (MQ=11)
                                                     aacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                                                          >  2:339001/4‑141 (MQ=255)
                                                       tGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                                                        <  2:459500/141‑1 (MQ=255)
                                                        ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAATAAACTGTCTACCGTTGGGTTCAAGAATATGTCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                       <  1:857155/141‑1 (MQ=255)
                                                        ggCTACTATCTAAGATATGAATTGAGCAATCGTGATGTTGCAGAAATAATAAGAGAACGTGATGTAAACGTTCATCGTTCAACGATCTACCGATGGGTTCAAGAATATGCCCCAATTTTATATGCAATTTGGAAGAAAAAg                                                                                       >  2:197515/1‑141 (MQ=11)
                                                         gccactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                      <  1:554228/138‑1 (MQ=25)
                                                         gCTACTATCTAAGATATGCCTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGTAGaaaagac                                                                                      >  2:504655/1‑138 (MQ=255)
                                                          ctactaTGTAAGAGATGCATTGAGCGATCGTGATATATCTGAAATATTAAGAGATCGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                     <  2:516851/141‑1 (MQ=11)
                                                          ctactaTCTAAGATAAGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGTAAAAGCa                                                                                     <  1:567772/141‑1 (MQ=11)
                                                           tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                    <  2:331155/141‑1 (MQ=35)
                                                           tactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                    <  2:497560/141‑1 (MQ=35)
                                                           tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                    >  2:256188/1‑141 (MQ=255)
                                                           tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGAGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTAAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCaa                                                                                    >  2:47677/1‑140 (MQ=11)
                                                            actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGCAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                  <  1:469355/141‑1 (MQ=255)
                                                             ccaTCTAAGATATGCATTGAGTTATCGTGATATATCCGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                 <  1:1588/139‑1 (MQ=21)
                                                               aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                               >  1:679852/1‑141 (MQ=35)
                                                               aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                               <  2:532801/141‑1 (MQ=255)
                                                               aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                               >  1:323143/1‑141 (MQ=255)
                                                               aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGCAAGAAAAAGCATaaaa                                                                               <  1:453592/141‑1 (MQ=255)
                                                                tCTAAGACATGCCTCGCGCTATCGTGATATATCTGAAATATTAAGCGACCGTGGTGCAAACGTTCATCATTCAACGCTCTACCGGTGGGTTCAAGAAAATGCCCCAATTTTATATCAAATGTGGAAGAAAAAGCATaaaaa                                                                              <  1:235452/141‑1 (MQ=11)
                                                                  tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGc                                                                            >  1:271531/1‑141 (MQ=37)
                                                                  tAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAgg                                                                            >  1:489989/1‑140 (MQ=255)
                                                                   aaGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc                                                                           <  2:393060/141‑4 (MQ=17)
                                                                   aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGTAAGAAAAAGCATaaaaaagc                                                                           >  2:560718/1‑138 (MQ=17)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          <  1:102322/141‑5 (MQ=11)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          >  2:427007/1‑137 (MQ=11)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          <  2:148993/141‑5 (MQ=255)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTGGGGTTCAAAAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          >  2:780100/1‑137 (MQ=11)
                                                                    aGATATGCATTGAGATATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAACGTTGGGTTCAATAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATGAAAAAGCt                                                                           >  2:624288/1‑141 (MQ=11)
                                                                    aGATATGACTTGAGTTATCGTGATATATATGAAATATTAAGGGAACGTGGTGTAAAAGTTAATAATTCCAAGGTATACCGTTGGGTGAAAGATTATGCACCACTTTTATATCTAATTTGGAACAAAAAGCAAAACAAGCTa                                                                          >  2:247762/1‑141 (MQ=2)
                                                                      atatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                                                        <  1:93010/141‑7 (MQ=1)
                                                                      atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                                                        >  2:911442/1‑135 (MQ=11)
                                                                      agaggcgtttggTTAACGTTATATATTTGAAATATTAAGGGAACGTGGTGAAAACGTTCATCATTCAACGGTCTACCGTTGGGGTGAACCATATGCCCGCATTTTATCTCAAATTTGGAAGAAAAAGCATAAAAAGATAAt                                                                        >  2:382655/12‑141 (MQ=11)
                                                                       tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                       >  1:820202/1‑134 (MQ=11)
                                                                               aagctATCGTGATATATCTGAAATATTAAGAGAACGTGGAGTAAACGTTCATCAATCAACGATCTACCGAAGGGTTCAAGAATATGCCCCAATTCAATACCAAATCTGGAAGAAAAAGCATAAAAAAACTAATTACAAAta                                                                >  1:41474/5‑140 (MQ=1)
                                                                                   tATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTa                                                           <  2:212114/141‑1 (MQ=40)
                                                                                     tCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAtt                                                         >  2:461817/1‑141 (MQ=39)
                                                                                                cTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                 <  1:760939/106‑1 (MQ=255)
                                                                                                                aCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGCAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGa                              >  2:363894/1‑141 (MQ=38)
                                                                                                                 cGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGaa                             >  1:715582/1‑141 (MQ=39)
                                                                                                                           aCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTa                   >  1:262597/1‑141 (MQ=38)
                                                                                                                              ttCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTAttt                <  2:571625/141‑1 (MQ=40)
                                                                                                                                          cGGTGTACCGTTGGATTCAATAAAATGCCCCAATTTTATATCAAATGTGTAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAt    <  2:444243/141‑1 (MQ=14)
                                                                                                                                            |                                                                                                                                             
GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTG  >  CP000730/76213‑76493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: