Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,411,914 G→T 7.5% P13Q (CCA→CAA)  USA300HOU_2277 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,411,9140GT 7.5% 294.2 / 2.0 102P13Q (CCA→CAA) USA300HOU_2277hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/55);  new base T (7/3);  total (44/58)
Fisher's exact test for biased strand distribution p-value = 9.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TCTATAATAAAATTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTATTCCCTTTTCA  >  CP000730/2411775‑2412051
                                                                                                                                           |                                                                                                                                         
tCTATAATAAAATTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTgg                                                                                                                                          <  1:735399/141‑1 (MQ=255)
tCTATAATAAAATTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTgg                                                                                                                                          >  1:676997/1‑141 (MQ=255)
        aaaaTTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGaaataaat                                                                                                                                  >  2:609561/1‑141 (MQ=255)
           aTTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCTGTTTGATATAAACCAt                                                                                                                               >  2:128247/1‑141 (MQ=255)
               gCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTgg                                                                                                                           >  2:744859/1‑141 (MQ=255)
               gCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTgg                                                                                                                           >  2:290239/1‑141 (MQ=255)
                 cATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGAt                                                                                                                         >  1:721872/1‑141 (MQ=255)
                  aTTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGAtt                                                                                                                        <  2:722220/141‑1 (MQ=255)
                  aTTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGAtt                                                                                                                        <  1:106887/141‑1 (MQ=255)
                   ttCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTc                                                                                                                       <  1:506115/141‑1 (MQ=255)
                   ttCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTc                                                                                                                       <  1:749788/141‑1 (MQ=255)
                   ttCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTc                                                                                                                       <  1:254224/141‑1 (MQ=255)
                   ttCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTc                                                                                                                       <  2:294877/141‑1 (MQ=255)
                       tatCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGTACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACAt                                                                                                                   <  1:860162/141‑1 (MQ=255)
                       tatCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACAt                                                                                                                   <  2:359903/141‑1 (MQ=255)
                         tCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCTTTTTGTCATAAATTATTCCGCTTCACATTa                                                                                                                 >  2:175309/1‑141 (MQ=255)
                         tCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTa                                                                                                                 <  1:845902/141‑1 (MQ=255)
                          cTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTAt                                                                                                                <  2:372273/141‑1 (MQ=255)
                           tACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATc                                                                                                               >  1:826699/1‑141 (MQ=255)
                           tACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATc                                                                                                               >  1:915697/1‑141 (MQ=255)
                           tACATGCATGATTAATCGTTCATTTGTTGCCAGAAATGCCCCTTCAAATTCATTCTCACCTTTAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTTAGTTGGAATTTAATCATTTGGATTCACATTAtt                                                                                                               >  2:803595/1‑140 (MQ=255)
                              aTTCATAATGAATCTGTCATTTGTTGCTAGAAATGCCACTTCAAAGTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTaa                                                                                                            <  1:729438/141‑1 (MQ=255)
                                tCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCTCCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCCTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTaaaa                                                                                                          <  2:355029/141‑1 (MQ=255)
                                tCATAAGTAATCTTTCATTAGTTGGTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTAGTCCTAGCACTGACGGACTCTTTTTTGCAGTTGGAAATAAATCATTTGGATTCACATTATCTaaaa                                                                                                          <  2:568964/141‑1 (MQ=255)
                                      ttaaaCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCGTTGTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCataa                                                                                                    >  1:620766/1‑141 (MQ=255)
                                       taatCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCataat                                                                                                   >  1:151245/1‑141 (MQ=255)
                                            tttCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATAtccc                                                                                              <  2:489761/141‑1 (MQ=255)
                                                ttttGTTGCTATAAATGCCCCTTCAAATTCCTTCTCCCCTTGAACTTGATACTCATTTATTCCTAGCACTTACGGACCCTTTTTTTCATTTTGAAATAAATCAGTTGTATTCACATTATCTAAAATCATAATAtccctccc                                                                                          <  2:902959/140‑1 (MQ=255)
                                                aTTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATAtccctccc                                                                                          <  2:888349/141‑1 (MQ=255)
                                                 tttGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCa                                                                                         <  2:329490/141‑1 (MQ=255)
                                                 tttGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAACCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCa                                                                                         <  1:371948/141‑1 (MQ=255)
                                                  ttgttgCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTTAGACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCAc                                                                                        >  1:480005/1‑141 (MQ=255)
                                                     ttgCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTa                                                                                     >  2:593394/1‑141 (MQ=255)
                                                      tgCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTaa                                                                                    <  2:538098/141‑1 (MQ=255)
                                                       tcTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTaaa                                                                                   <  1:286824/140‑1 (MQ=255)
                                                       gCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGCACATATTCTTGGTGTGTCACATTATCTAAAATCATAATATCCCTCCCACTTaaa                                                                                   >  1:295975/1‑141 (MQ=255)
                                                       gCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTaaa                                                                                   <  1:61407/141‑1 (MQ=255)
                                                            aaaTGCCCCTTTAAATTCATTCTCACCTTGAACTTTATACTCAATTATTCCTAGCCCCGACGGACCCTTTTTTTCAGTTTGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactat                                                                              <  2:513104/141‑1 (MQ=255)
                                                            aaaTGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactat                                                                              <  1:609561/141‑1 (MQ=255)
                                                              aTGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactataa                                                                            <  1:855289/141‑1 (MQ=255)
                                                              aTGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactataa                                                                            >  1:882673/1‑141 (MQ=255)
                                                               tGCCCCTTTAAATTCATGCTCGCCTTGAACTTGATACTGAATTATTCCTATCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCAATTGGATTCACATTATCTAAAATCATAATAGCCCTCCCACTTAaaactataaa                                                                           <  2:450090/141‑1 (MQ=255)
                                                               tGCCCCTTCAATTCCATTCTCTCCTTGAACTTGATACTCTATTATTCCTAGCACTGACTGACCCCTTTTTTCAGTTGGAAATAAATCATTTTGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAcaactataaa                                                                           <  2:258092/141‑1 (MQ=255)
                                                               tGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCAATTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactataaa                                                                           <  2:178974/141‑1 (MQ=255)
                                                                gCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactataaac                                                                          <  2:589255/141‑1 (MQ=255)
                                                                  cccTTTAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGTACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAaaactataaacta                                                                        <  2:321614/141‑1 (MQ=255)
                                                                  cccTTCAAATTCATCCTCACCTTGAACTTGATACTCAATTATTCCGAGCACTGACAGACCCCTTTTTACAGTTGGAAATAAACCATTTGGATTCACATTATCTAAAATCATACTATCCCTCCCACTTAatactataaacta                                                                        <  2:424100/141‑1 (MQ=255)
                                                                     ttCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTATCACTGACGGACCCTTTTTTTTTTTTTTTTTTTAATCATATGGATTCACATTATTTAAGATCATAATATCCGTCCCCCTTTAAACTATAAACttttt                                                                     >  2:485095/1‑140 (MQ=255)
                                                                      tCAATTTCATTCTCACCTTGAACTTTATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGTATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTAttct                                                                    <  2:490111/141‑1 (MQ=255)
                                                                      tCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCCAAAATCCTACTATCCCTCCCCCGGAAAACTACAAACAActct                                                                    >  1:427498/1‑141 (MQ=255)
                                                                       cAAATTCATTTTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTAttctt                                                                   <  2:530752/141‑1 (MQ=255)
                                                                       cAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTTTTTTTTTAATAAATCATTTGGATTCACATTATCTAAAATCATAATATGCCTCCCACTTAAAACTATAACCTAtcctt                                                                   >  2:148933/1‑141 (MQ=255)
                                                                       cAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTAttctt                                                                   >  2:103689/1‑141 (MQ=255)
                                                                       cAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTAttctt                                                                   >  2:103680/1‑141 (MQ=255)
                                                                        aaaTTCATTCTCACCTGGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCCCCCACATAAAACTATAAACTAatcttc                                                                  >  2:154117/1‑141 (MQ=255)
                                                                         aaTTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTAttcttta                                                                 >  1:355944/1‑139 (MQ=255)
                                                                             cattcTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAgg                                                             <  2:681728/141‑1 (MQ=255)
                                                                                tctcACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGtat                                                          >  1:536908/1‑141 (MQ=255)
                                                                                 ctcACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGtata                                                         >  1:825707/1‑141 (MQ=255)
                                                                                   cACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATg                                                       <  1:67636/141‑1 (MQ=255)
                                                                                    cccTTGAACTTGTTACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTACAACTATAAACCCTTCTTCATAGGTATATGa                                                      <  2:82184/140‑1 (MQ=255)
                                                                                      cTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGaaa                                                    <  2:289949/141‑1 (MQ=255)
                                                                                        tGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCGTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATa                                                  <  2:485099/141‑1 (MQ=255)
                                                                                        tGAACTTGATACCCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATa                                                  <  2:316479/141‑1 (MQ=255)
                                                                                        tGAACTTGAAACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATa                                                  <  2:835734/141‑1 (MQ=255)
                                                                                           aCTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACaa                                               <  2:730152/141‑1 (MQ=255)
                                                                                            cTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAg                                              <  1:697358/141‑1 (MQ=255)
                                                                                            cTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAg                                              >  2:126156/1‑141 (MQ=255)
                                                                                               gATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTTTTTTTTTTTTAAATAATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCTCTTAAAACTATAAACTTTTCTTCATAGTTATATGAAATACAAGTgt                                           >  1:350643/1‑140 (MQ=255)
                                                                                               gATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTTTTTTTTAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATAAAAttaaattgt                                           >  2:224188/1‑133 (MQ=255)
                                                                                               gATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTgt                                           >  1:329855/1‑140 (MQ=255)
                                                                                               gATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGa                                           >  1:637414/1‑141 (MQ=255)
                                                                                               gATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTATTAGGTTTATGAAATACAAGTgt                                           >  2:341071/1‑140 (MQ=255)
                                                                                                      cattattCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACta                                    <  1:192311/140‑1 (MQ=255)
                                                                                                      aaTTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACta                                    >  1:107388/1‑141 (MQ=255)
                                                                                                         tattCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACtattt                                 >  1:820712/1‑141 (MQ=255)
                                                                                                             ccTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATttatta                             >  1:909418/1‑141 (MQ=255)
                                                                                                               tAGCACTGACGGAACAAATCTCACAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCACTCCCCCTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAATGATTAACTATttattatt                           >  2:883427/1‑141 (MQ=255)
                                                                                                                 gCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTaa                         <  1:103680/141‑1 (MQ=255)
                                                                                                                 gCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTaa                         <  1:148933/141‑1 (MQ=255)
                                                                                                                 gCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTaa                         <  1:103689/141‑1 (MQ=255)
                                                                                                                  cACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAg                        >  1:187215/1‑141 (MQ=255)
                                                                                                                  cACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAg                        <  2:151245/141‑1 (MQ=255)
                                                                                                                   aCTGACGGACCCTTTTTTTTTTTTTTTTTTAACCCATTTGGATTCACATTATCTAAAATAATAATATCCCTCCCAACTAAAACTATACCCTATTCTTCTTAGGTATATTAAATCCACTTGATTATCTTTTTATTATTAAGa                       >  2:394421/1‑141 (MQ=255)
                                                                                                                   aCTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGa                       >  1:359127/1‑141 (MQ=255)
                                                                                                                   aCTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGa                       >  1:232397/1‑141 (MQ=255)
                                                                                                                       accgACCCTTTTTTTCAGTTTGAAAGAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTaa                   <  2:826699/138‑1 (MQ=255)
                                                                                                                        ccggcgcTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAATCTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAAc                  <  2:676997/135‑1 (MQ=255)
                                                                                                                         ggACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACt                 <  2:695627/141‑1 (MQ=255)
                                                                                                                          gACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACtt                >  1:899388/1‑141 (MQ=255)
                                                                                                                          gACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACtt                >  2:185086/1‑141 (MQ=255)
                                                                                                                          gACCCTTTTTTTCAGTTGGAAATAAATCATGTGGATTCACATTATCTAAAATCAGAATATCCCTCCCACTTAAAACTATAAACTATTCTTAATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAATATTAACtt                <  2:670942/141‑1 (MQ=255)
                                                                                                                          gACCCTTTTTGTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACtt                <  2:113265/141‑1 (MQ=255)
                                                                                                                            cccTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATGAACTATTTATTATTAAGATTAACTTGt              >  1:21448/1‑141 (MQ=255)
                                                                                                                               tttttttCAGTTGGAAATAAAGCATTTGGATGCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTAtt           <  1:535655/141‑1 (MQ=255)
                                                                                                                                   tttCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTATTCCCt       <  1:525917/141‑1 (MQ=255)
                                                                                                                                   tttCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTATTCCCt       <  1:75887/141‑1 (MQ=255)
                                                                                                                                       aGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTACATATTTATTATTAAGATTAACTTGTATTCCCTTTTc   <  1:216713/141‑1 (MQ=255)
                                                                                                                                        gTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATCCAAGTGATTAACTATTTATTATTAAGATTAAGTTCTATTCCCTTTTCa  >  2:330195/1‑141 (MQ=255)
                                                                                                                                        gTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTATTCCCTTTTCa  >  1:693706/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TCTATAATAAAATTGGCCATTCATATCTACATTCATAATTAATCTTTCATTTGTTGCTATAAATGCCCCTTCAAATTCATTCTCACCTTGAACTTGATACTCAATTATTCCTAGCACTGACGGACCCTTTTTTTCAGTTGGAAATAAATCATTTGGATTCACATTATCTAAAATCATAATATCCCTCCCACTTAAAACTATAAACTATTCTTCATAGGTATATGAAATACAAGTGATTAACTATTTATTATTAAGATTAACTTGTATTCCCTTTTCA  >  CP000730/2411775‑2412051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: