Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 68,674 (AAT)7→6 5.4% coding (790‑792/852 nt) USA300HOU_0065 → transposase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 68659 =57 (0.750)3 (0.050) 3/224 NT 5.4% coding (775/852 nt) USA300HOU_0065 transposase
?CP000730 = 68655 57 (0.870)coding (771/852 nt) USA300HOU_0065 transposase

AATTACCTTTATAAACAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/68816‑68659
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attattattattattattGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGACTTTAGCGAGGAATGGAAACTTTCTATCGGGGCGTTATCGGCAGGTGTTCCC  <  CP000730/68655‑68523
                                                                                                                                                                                                                                                                                                   
AATTACCTTTATAAACAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGA                                                                                                                                                        >  1:865647/1‑141
  TTAACTTTATAAACAAAAGAGCCTATAAGCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATT                                                                                                                                                      <  1:405052/141‑1
               CAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAACCACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGTTAAGTGGCCTAGGTTTTGTTGACTTCGATTTTTCTTATTATTA                                                                                                                                         >  1:40525/1‑141
               CAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGACTTCGATTATTATTATTATTA                                                                                                                                         >  1:282856/1‑141
               CAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTA                                                                                                                                         >  1:846538/1‑141
               CAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTA                                                                                                                                         >  1:278539/1‑141
                aaaatgGCCTATAAGCCCAGGGCACTGAACCCTTAAAACGGGAACTTAATAAACACGCCATGTTCTAGGCTATTATTTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATACGATTATGATTATTATTAT                                                                                                                                        <  2:786672/135‑1
                AAAAGAGCGTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTATTTTTTGAATTCGATTATTATTATTATTAT                                                                                                                                        <  2:602254/141‑1
                                CTAGGGCACTTAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTATTTTTTCTAATGTACTT                                                                                                                        <  2:618117/141‑1
                                                                          CATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTATTGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTC                                                                              <  1:238835/141‑1
                                                                                                   TATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTATTGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGAC                                                     >  2:896026/1‑141
                                                                                                                                            ATTATTATTATTATTATTGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGACTTTAGCGAGGAATGGAAACTTTCTATCGGGGCGTTATCGGC            >  2:375269/1‑141
                                                                                                                                                 TATTATTATTATTGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGACTTTAGCGAGGAATGGAAACTTTCTATCGGGGCGTTATCGGCAGGTG       >  1:158120/1‑141
                                                                                                                                                      TTATTATTGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGACTTTAGCGAGGAATGGAAACTTTCTATCGGGGCGTTATCGGCAGGTGTTCCC  <  2:452619/141‑1
                                                                                                                                                                                                                                                                                                   
AATTACCTTTATAAACAAAAGAGCCTATAATCCTAGGGCACTGAACCCTTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTAATTCTCTGTATTTTACAGGGGATAAGTAGCCTAGTTTTTGTTGAATTCGATTATTATTATTATTATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/68816‑68659
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attattattattattattGTTTTTAATGTACTTTTCGACAATATCTATTACAATGTGATTAGAGTGATTAAGCTCATTATTGATGTAAAAAGTTTCAGACTTTAGCGAGGAATGGAAACTTTCTATCGGGGCGTTATCGGCAGGTGTTCCC  <  CP000730/68655‑68523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.