Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 45.0 / NA 16I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/5);  total (11/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416346‑1416594
                                                                                                                |                                                                                                                                        
ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGGTTAATATTACCTCGCTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGaat                                                                                                              >  1:83226/1‑139 (MQ=255)
ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                              >  1:30849/1‑141 (MQ=255)
             cTATATTTAATTAGACGTTCAGTCTACGGATGCAAATGCTCATTCTTTAAATGATTAGTATTACCACGTTCAATGATTTGACCTTCTTTTAAAACATATTTTTACTAACAATATTTCAATACATGACTTAAGtagtgtcaa                                                                                                 >  2:211226/1‑133 (MQ=255)
                  tttAATTAGACGTTCAGTAAACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataat                                                                                            >  1:150759/1‑141 (MQ=255)
                               tCAGTATACGGATGAATATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATCTTTCAATACAGGACGTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                               <  2:15315/141‑1 (MQ=255)
                                    ataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                          >  1:76696/1‑141 (MQ=255)
                                                                 gATTAATATTACCTCGTTCAATGATTTGACATTCTTATAAAACATAATTGTACCGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACAATGTTCTAATACAATATGCTGTAATAAATCCa                                             >  1:105037/1‑141 (MQ=255)
                                                                   ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                           >  1:56103/1‑141 (MQ=255)
                                                                              tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                                >  1:75401/1‑141 (MQ=255)
                                                                                gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGt                              <  2:211384/141‑1 (MQ=255)
                                                                                    aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATCCTGTAATAAATCCATCACTTGATTACCGTTCaa                          >  2:112927/1‑141 (MQ=255)
                                                                                                ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga              <  2:243107/141‑1 (MQ=255)
                                                                                                ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatga              <  2:243077/141‑1 (MQ=255)
                                                                                                   ttttAAAAAATAATTATAATGACAATATCTAAATACATGACTCAAGTTATGCGAGATAATAAATAATGTTTGACCACGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGc           <  1:11469/141‑1 (MQ=255)
                                                                                                      tAAAACATAATTGTACTGACAATATTTTAATACATGACTTAAGTTATGTGTGATAATCAATAATGTTTGACCATGTTCTTATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCacc        >  2:102364/1‑139 (MQ=255)
                                                                                                            aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTATAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  >  2:80466/1‑141 (MQ=255)
                                                                                                                |                                                                                                                                        
CTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416346‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: