Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,002,200 T→G 100% M173R (ATG→AGG)  mecA2 → competence negative regulator MecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,002,2000TG97.9% 74.8 / ‑6.2 25M173R (ATG→AGG) mecA2competence negative regulator MecA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (12/12);  minor base C (1/0);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCT  >  CP000730/1002064‑1002336
                                                                                                                                        |                                                                                                                                        
ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                      >  2:13148/1‑141 (MQ=255)
ggTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATATGTTATCTAGGGtt                                                                                                                                      >  2:160680/1‑141 (MQ=255)
       aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTCATTATGCATATCATATCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                                                                               <  2:244937/141‑1 (MQ=255)
         cGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGTTACt                                                                                                                             >  2:249752/1‑141 (MQ=255)
                aGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatt                                                                                                                      >  2:182302/1‑141 (MQ=255)
                            gaaaaaaGACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                                                                          <  1:78863/139‑1 (MQ=255)
                            gTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                                                                          <  1:13148/141‑1 (MQ=255)
                             tAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATCCTGTATAttt                                                                                                         >  2:161181/1‑141 (MQ=255)
                               aaaaTACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTg                                                                                                       <  1:124157/141‑1 (MQ=255)
                                                  tgtgAAATTTAACGATTAAGTGGAAGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAGCaaga                                                                                    >  2:124905/1‑141 (MQ=255)
                                                     gAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGATATCATCGTTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                                                                                 >  1:264500/1‑141 (MQ=255)
                                                           tAATGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGTTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTaa                                                                           <  1:231746/141‑1 (MQ=255)
                                                                             tattaATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGGTGATCAAGAAGTCATTAATGCTATTTACAGTCAAtt                                                         >  2:179834/1‑141 (MQ=255)
                                                                                taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                      <  2:90570/141‑1 (MQ=255)
                                                                                taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                      <  2:232638/141‑1 (MQ=255)
                                                                                        cATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGTGATTATGTGACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAGTTGTTTGAAtttt                                              >  2:56997/1‑140 (MQ=255)
                                                                                              aTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATc                                        >  2:83524/1‑141 (MQ=255)
                                                                                                               aCAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGAcagaacagaa                       >  2:215108/1‑141 (MQ=255)
                                                                                                                cAGAGTTTGAAGATTTGTTATATAGGGTTGATGGGACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACACAcaaaacataaa                      >  2:88070/1‑140 (MQ=255)
                                                                                                                 agagTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGt                     >  2:73268/1‑141 (MQ=255)
                                                                                                                    gTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGttta                  <  1:152539/141‑1 (MQ=255)
                                                                                                                           gATCTGTTATATAGGGTTGATGGTACTTATTATTACGTTGTATATGTGGATCTTCATGATGATCCAGCGGTTCTTCAAGTGCCATCCTTTCATTTGCTTGAATGTTCTTATCCAAGAGACGGAATAGATGTTTACTTAAAt           <  2:67429/141‑1 (MQ=255)
                                                                                                                              ttGTTATATAGGGTTGATTGTACTTATTATTATGCTTTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGAc        <  1:151692/141‑1 (MQ=255)
                                                                                                                              ttGTGATATAGGGTTGATGGTACTTATTATTATGCTGTATCATTTGCTAGTCATGTTGATCAAGCAGTTCGTCATGATAGTTACTTCGACCTGCGTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGAc        <  1:63369/141‑1 (MQ=255)
                                                                                                                                  tatataGGGTTGATGGTACTTATGATTATGTTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTCGCTTATCCAACAGAATTTACAGAAGTTTATTTAAATGACTATg    <  2:124224/141‑1 (MQ=255)
                                                                                                                                    tatagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCt  <  2:264500/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCT  >  CP000730/1002064‑1002336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: