Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6980CA90.0% 211.1 / 19.9 70coding (1216/1260 nt)USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (35/27);  minor base . (5/2);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*CP0007302,455,6990AT100.0% 208.4 / ‑6.5 72H406L (CAT→CTT) USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (39/29);  total (40/32)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC  >  CP000730/2455568‑2455831
                                                                                                                                   ||                                                                                                                                    
aGTTAACAAAGCACCACAAAGTGACGATTTAACAAACGATGCAAATGAAGATGAAATAGAGCACTCAATCAATGAGCATGCGTTTGAAGAATAACACAGTCGTTGCGAAAGTGTAATTGATGCAAAAAAATTATCGataaa                                                                                                                            <  2:743978/141‑1 (MQ=255)
     aaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGCCGTTCATCAGTTGTAATTGATGCAAAAAAATTATCCAAAAAACTga                                                                                                                       >  2:21789/1‑141 (MQ=255)
         aGCACCAAAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTACAAAGAAGACAGACGTTCATCAGTTGTAATCGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                                   <  1:555262/141‑1 (MQ=255)
          gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaaa                                                                                                                  >  1:383499/1‑140 (MQ=255)
          gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAAc                                                                                                                  >  2:587125/1‑141 (MQ=255)
          gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGAAAGACCGACAGCAGTTGTAATTGATGCAGAAAAATTATCGAAAAAACTGAAGAAc                                                                                                                  >  1:175028/1‑141 (MQ=255)
             ccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATGATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTGAAGAACAAt                                                                                                               <  2:709651/141‑1 (MQ=255)
              cacaAAGTAACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATc                                                                                                              <  2:514938/141‑1 (MQ=255)
               acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                             <  2:71951/141‑1 (MQ=255)
                 aaaGAGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTc                                                                                                           >  1:526628/1‑141 (MQ=255)
                   aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCaa                                                                                                         >  1:346906/1‑140 (MQ=255)
                      gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATa                                                                                                      >  2:534433/1‑141 (MQ=255)
                       aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAGTATCGAAAACACTGAAGAACAATCTTCAGATaa                                                                                                     >  1:274004/1‑141 (MQ=255)
                        cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                    <  1:65576/141‑1 (MQ=255)
                        cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                    >  1:505645/1‑141 (MQ=255)
                         gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                   >  1:206970/1‑141 (MQ=255)
                                   aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata                                                                                         >  2:739394/1‑141 (MQ=255)
                                     gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTAAAGAAAAATCTTCAGATAAAAACAAATAATAAc                                                                                       >  1:262771/1‑141 (MQ=255)
                                     gATTCAAAGGAAGATGAAATAGAGCAAGCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc                                                                                       <  2:133120/141‑1 (MQ=255)
                                      aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa                                                                                      <  2:639484/141‑1 (MQ=255)
                                        tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaa                                                                                    >  2:750263/1‑141 (MQ=255)
                                         cAAATGAAGATTAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                                   <  1:505909/141‑1 (MQ=255)
                                         cAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                                   >  1:99912/1‑141 (MQ=255)
                                         cAAAAGAAGATGAAAGAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                                   <  2:381049/141‑1 (MQ=255)
                                            aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcgc                                                                                <  1:357924/141‑1 (MQ=255)
                                                    gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                        >  2:576516/1‑141 (MQ=255)
                                                    gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                        >  2:576507/1‑141 (MQ=255)
                                                    gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                        >  2:245158/1‑141 (MQ=255)
                                                    gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                        >  2:576138/1‑141 (MQ=255)
                                                      aaaagagAAATCAACAAAAGAAACGGCGACCTAACAAAAAGACAGACGTTCATTAGTTGTAATTGATGCAAAAAAATTTTCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATCACAAAGCGCTTGCTAGTAc                                                                      <  1:312134/138‑1 (MQ=255)
                                                        tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGCCAAACTTGATGATCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct                                                                    <  2:162554/141‑1 (MQ=255)
                                                        tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCGAGAAccc                                                                    >  1:168030/1‑140 (MQ=255)
                                                          gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGCTAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                                  >  1:70573/1‑141 (MQ=255)
                                                          gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACctct                                                                  >  2:215780/1‑141 (MQ=255)
                                                           agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCGCtt                                                                 >  2:673295/1‑141 (MQ=255)
                                                           agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCGTGCTAGTACCTCtt                                                                 >  1:47117/1‑141 (MQ=255)
                                                            acgagcagtAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAGCGCTTGCTAGTACCTCTTa                                                                <  2:189002/133‑1 (MQ=255)
                                                              aagcagtAAAACGACCCGCGACTTATAAAGAAGAAAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                              <  2:212116/135‑1 (MQ=255)
                                                               aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                             <  2:646416/141‑1 (MQ=255)
                                                                 cgtaaaaaCAACCGGCGACTTACAAAGAAAACAAACGGTCATCATAAATAATTGATGCAAAAACAATACCGAAAAGACAGAATCACAACCTTCAGATAAAAACAAGTAATAACAAAGCTCTTGCTAGTACCTCTTaaaaat                                                           <  2:274004/137‑2 (MQ=255)
                                                                 cATTAAAAGAACCTGAGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                           >  2:138325/1‑141 (MQ=255)
                                                                    tAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAACCTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCACGTGCTAGTACCTCTCAAAAAcata                                                        >  2:365039/1‑140 (MQ=255)
                                                                     aaaaGAACCTGCGACTAATAAAGAAAACAGACGTTCATCAGTAGAAATAGAAAAAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                       <  2:429024/141‑1 (MQ=255)
                                                                         aaaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                                   <  1:158598/140‑1 (MQ=255)
                                                                            ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAAAAATAATAACAAAGCGCTAGCACGTACCTCTTAAAAAGAGAATGCTAGc                                                >  2:359595/1‑141 (MQ=255)
                                                                               gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                             <  2:557410/141‑1 (MQ=255)
                                                                               gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTCGCAAg                                             >  2:586966/1‑141 (MQ=255)
                                                                               gCGACCTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAGAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAg                                             <  2:538099/141‑1 (MQ=255)
                                                                                cGACTGATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTGAAAAAGATCATGCTAGCAAgc                                            <  1:400622/141‑1 (MQ=255)
                                                                                   ttaataAAGAAGACAGACGTTCATCAGTTGCAATCGATGCAAAAAAACTATCGAAAAAACTGAAGAACAAAAATAAGATAAAAACAAATAATAACAAAGCGCTTTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                         <  2:583345/138‑1 (MQ=255)
                                                                                   cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                         <  1:321379/141‑1 (MQ=255)
                                                                                    ttATAAATAAGACAGACTTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt                                        <  2:612835/141‑1 (MQ=255)
                                                                                    ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTACTAGTACCACTTAAAAAGATGATGCTAGAGAGGGCtt                                        >  1:4894/1‑141 (MQ=255)
                                                                                            aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAAGATGCCAGCAAGCGCTTTTctatac                                <  1:570221/141‑1 (MQ=255)
                                                                                             agacagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAACATGATGCTAGCAAGCGCTTTTctatact                               >  1:537871/1‑141 (MQ=255)
                                                                                               acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAGGCGCTTTTctatactat                             >  1:613049/1‑141 (MQ=255)
                                                                                               acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                             >  1:148551/1‑141 (MQ=255)
                                                                                               acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                             >  1:368960/1‑141 (MQ=255)
                                                                                               acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAAACTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTCAAAAGATGATGCTAGCCAGGGCATTTctatcccat                             >  1:238008/1‑136 (MQ=255)
                                                                                                 agacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAATACCTCTTAAAAAGATGATGCTAGCAAGCCCTTTTCTATACtatat                           >  2:96330/1‑141 (MQ=255)
                                                                                                   acGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCCGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATActatctat                         >  2:520043/1‑141 (MQ=255)
                                                                                                    cGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt                        >  1:391133/1‑141 (MQ=255)
                                                                                                       tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                     <  2:99912/141‑1 (MQ=255)
                                                                                                          atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt                   <  1:476499/141‑1 (MQ=255)
                                                                                                          atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt                   <  2:505645/141‑1 (MQ=255)
                                                                                                           tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt                  >  1:265571/1‑141 (MQ=255)
                                                                                                           tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTCGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCCTATATATTAttttt                  >  2:98962/1‑141 (MQ=255)
                                                                                                            caGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc                 >  1:21740/1‑141 (MQ=255)
                                                                                                                   aaTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt          >  2:683375/1‑141 (MQ=255)
                                                                                                                     ttGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt        <  2:563503/141‑1 (MQ=255)
                                                                                                                          tgcaaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca    <  1:252175/137‑1 (MQ=255)
                                                                                                                            caaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATCAATATTTTGTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAcc  <  1:177625/139‑1 (MQ=255)
                                                                                                                                   ||                                                                                                                                    
AGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACC  >  CP000730/2455568‑2455831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: