Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,196,508 C→A 100% intergenic (+2563/‑1229) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,196,5080CA98.8% 253.4 / ‑5.5 84intergenic (+2563/‑1229)ftsQ/USA300HOU_1125cell division protein FtsQ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (33/48);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAG  >  CP000730/1196371‑1196643
                                                                                                                                          |                                                                                                                                       
gATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAg                                                                                                                                      <  2:394756/141‑1 (MQ=255)
  ttttCGGTCCAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAATTATTGCAACTGGTTTTGATGACAAACCAACATCAGATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCa                                                                                                                                    >  2:569642/1‑141 (MQ=255)
  ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTTGAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCa                                                                                                                                    >  2:3657/1‑141 (MQ=255)
  ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAgaa                                                                                                                                    >  2:223089/1‑139 (MQ=255)
  ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCa                                                                                                                                    >  2:35220/1‑141 (MQ=255)
  ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCa                                                                                                                                    <  2:24574/141‑1 (MQ=255)
  ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCa                                                                                                                                    >  2:694621/1‑141 (MQ=255)
   tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCCCTGGATTCGGAACAAGCGTAAATACTTATAGCaa                                                                                                                                   <  1:33016/141‑1 (MQ=255)
      cGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGc                                                                                                                                <  2:488857/141‑1 (MQ=255)
        gTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACGTATAGCAATGGaa                                                                                                                              >  1:217721/1‑141 (MQ=255)
        gTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATAATTATAGCaacggtc                                                                                                                              >  2:574895/1‑136 (MQ=255)
         tACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAAc                                                                                                                             <  2:26841/141‑1 (MQ=255)
              ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACGTCTa                                                                                                                        <  1:528074/141‑1 (MQ=255)
                attaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAAAAAGAGTAAAAACGTATAGCAATGCAAATTCTaaa                                                                                                                      >  2:45121/1‑141 (MQ=255)
                 ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg                                                                                                                     >  1:745876/1‑141 (MQ=255)
                 ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg                                                                                                                     >  1:79864/1‑141 (MQ=255)
                 ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGAAACTTCTAAAg                                                                                                                     >  1:745864/1‑141 (MQ=255)
                    aTCCTGCATTACAAGATGAGATTATAGTAACAGATATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATg                                                                                                                  >  2:452242/1‑141 (MQ=255)
                           aTTACAAGATTAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATTACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                           <  1:401911/141‑1 (MQ=255)
                           aTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                           <  1:200047/141‑1 (MQ=255)
                            ttACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                          >  2:359011/1‑141 (MQ=255)
                            ttACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc                                                                                                          <  2:248450/141‑1 (MQ=255)
                                aaGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt                                                                                                      >  1:75789/1‑141 (MQ=255)
                                aaGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt                                                                                                      <  1:747522/141‑1 (MQ=255)
                                 caaTGAGATTGTTGTAACAGTCATTGCAACTTGTTTCGATGACAACCCAACATCACATGGTCGTACATCTGGTAGCACTTGATTCGGAACAAGCGTACACACTTATAGCAATGCAACTTCTAAAGACGAATCATTCACTTc                                                                                                     <  2:624854/139‑1 (MQ=255)
                                 aGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGTCAAACAACCATCACACGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTc                                                                                                     <  1:213760/141‑1 (MQ=255)
                                 aGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTc                                                                                                     <  1:710020/141‑1 (MQ=255)
                                        aTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTGGCACTGGATTCGGAACAAGCGGAAATACTTATAGCAATGCAACTTCCAAAGAAGAAGCAttagatgcaaagtca                                                                                              >  1:558097/1‑128 (MQ=255)
                                        aTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtca                                                                                              >  1:776016/1‑141 (MQ=255)
                                           gttgtAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGCTGACTCATTCACTACAAATtcatca                                                                                           >  2:697524/1‑141 (MQ=255)
                                               tAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAGAGTCGAATCATTCACTTCTGATTCATCAAATg                                                                                       <  2:130025/141‑1 (MQ=255)
                                                aaCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGc                                                                                      >  1:442600/1‑141 (MQ=255)
                                                aaCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGc                                                                                      >  1:442632/1‑141 (MQ=255)
                                                   aGTTATTGCAACTGGTTTTGATTACAAACCAACATCACATGGTCGCAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTAGAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca                                                                                   <  2:42591/141‑1 (MQ=255)
                                                   aGTTATTGCAACTGGTTTTGATGACAAACCAATATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca                                                                                   <  1:344127/141‑1 (MQ=255)
                                                   aGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca                                                                                   <  1:666243/141‑1 (MQ=255)
                                                   aGTTATTGCAACTGGCTTTGATGACAAACCAACATCATATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTTATCAAATTcaca                                                                                   <  1:546161/141‑1 (MQ=255)
                                                   aGTTATTGCAACTGGATTTGATGACAAACCAACATCATATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATCCAACTTCTAAAGATGAATCATTCACTTCAAATTTATCAAATTcaca                                                                                   <  1:546097/141‑1 (MQ=255)
                                                    gTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAAACTGGTAGCACTGGACTCGGAATAAGCGTAAATACTTCTGGCAATGCAACTTCTAAAAATCAATCAAGCTCTTCCAATTCTTCCAATGTACaa                                                                                  >  2:313842/1‑141 (MQ=255)
                                                     ttATTGCCACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACATGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAg                                                                                 <  2:26655/141‑1 (MQ=255)
                                                      tATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGc                                                                                >  2:573660/1‑141 (MQ=255)
                                                       aTTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCa                                                                               <  1:563439/141‑1 (MQ=255)
                                                        ttGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCaa                                                                              <  1:450285/141‑1 (MQ=255)
                                                         tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc                                                                             <  1:704765/141‑1 (MQ=255)
                                                         tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc                                                                             <  1:654487/141‑1 (MQ=255)
                                                           cAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACACGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTg                                                                           <  2:370532/141‑1 (MQ=255)
                                                              cTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATCGCACTACAGCTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGTTa                                                                        >  1:177489/1‑141 (MQ=255)
                                                                 ggtttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGCCACGGGACTAGTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg                                                                     <  1:367527/139‑1 (MQ=255)
                                                                 gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAATCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCACATGCACAAGCAACTGATAgtg                                                                     <  1:399160/141‑1 (MQ=255)
                                                                    ttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTaa                                                                  <  1:649177/141‑1 (MQ=255)
                                                                      gatgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGt                                                                >  1:769950/1‑141 (MQ=255)
                                                                       atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg                                                               >  1:636924/1‑141 (MQ=255)
                                                                       atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg                                                               >  1:438992/1‑141 (MQ=255)
                                                                         gaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGCATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTTTAAGTGaa                                                             >  2:508190/1‑141 (MQ=255)
                                                                          aCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaaa                                                            >  1:2718/1‑141 (MQ=255)
                                                                           cAAACCAACATCACATGGTCGTAAATCTGGTAGCCCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAg                                                           <  2:718907/141‑1 (MQ=255)
                                                                           cAAAACAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATTAAATGCACAAGCAACTGATAGTGTAAGTGAAAg                                                           >  1:525677/1‑141 (MQ=255)
                                                                             aaCCAACATCACATGGTCCTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGaa                                                         <  2:375361/141‑1 (MQ=255)
                                                                               ccAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAaca                                                       >  2:62313/1‑141 (MQ=255)
                                                                                   cATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATa                                                   <  1:45025/141‑1 (MQ=255)
                                                                                   aaTCACAGGGTCATAAATCTGGTAGCACGGGATTCGTAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATa                                                   <  1:748714/140‑1 (MQ=255)
                                                                                     tCACGTGGTCGTAAATATAGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGACGAAGCAAAGGTTAGTGTAAGTGAAAGAAAACATACa                                                 >  2:159174/1‑141 (MQ=255)
                                                                                     tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGACAGAAAACATACa                                                 >  2:33633/1‑141 (MQ=255)
                                                                                     tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa                                                 <  1:213186/141‑1 (MQ=255)
                                                                                     tCACATGATCGTAAATCTGGCAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa                                                 >  1:485696/1‑141 (MQ=255)
                                                                                          tGGTCGTAAATCTGGTAGGACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTaa                                            <  2:182508/141‑1 (MQ=255)
                                                                                            gTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaag                                          <  1:306940/141‑1 (MQ=255)
                                                                                               gTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaagaag                                       <  1:515111/141‑1 (MQ=255)
                                                                                                tAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAagaaga                                      <  1:44529/141‑1 (MQ=255)
                                                                                                   aTCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga                                   >  1:701647/1‑141 (MQ=255)
                                                                                                   aTCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga                                   <  2:324969/141‑1 (MQ=255)
                                                                                                        gTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc                              <  1:723624/141‑1 (MQ=255)
                                                                                                        gTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc                              <  1:723636/141‑1 (MQ=255)
                                                                                                         tAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTcc                             <  1:604272/141‑1 (MQ=255)
                                                                                                          aGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAACGAAAACATACAACTAAAGAAGATGATATGCCt                            >  1:330762/1‑141 (MQ=255)
                                                                                                              cTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAAATGATATTCCTAGCt                        <  1:573660/141‑1 (MQ=255)
                                                                                                                ggATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTACAGATGAATCATTCACTTCAAACTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAACGAACCCATAAAACTAAAGCAGATGATATTCCTAGCTTc                      <  2:102138/141‑1 (MQ=255)
                                                                                                                            aaGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATag           <  1:359011/141‑1 (MQ=255)
                                                                                                                              gCGTAAATACTTTCAGCCATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATagag         <  2:558097/141‑1 (MQ=255)
                                                                                                                              gCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATagag         <  2:776016/141‑1 (MQ=255)
                                                                                                                                  aaaTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGagaaga     >  1:647641/1‑141 (MQ=255)
                                                                                                                                     tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag  <  2:657084/141‑1 (MQ=255)
                                                                                                                                     tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag  <  1:379673/141‑1 (MQ=255)
                                                                                                                                     aaCTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag  <  2:660223/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAG  >  CP000730/1196371‑1196643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: